Facsaria Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Regulation of Epstein-Barr Virus Life Cycle and Cell Proliferation by Histone H3K27 Methyltransferase EZH2 in Akata Cells"
Article Title: Regulation of Epstein-Barr Virus Life Cycle and Cell Proliferation by Histone H3K27 Methyltransferase EZH2 in Akata Cells
Figure Legend Snippet: Induction of the EZH2 gene by Epstein-Barr virus (EBV) infection in primary B cells. (A) B cells isolated from peripheral blood mononuclear cells from a healthy donor were sorted using FACSAria II and infected or mock infected with WT EBV at a multiplicity of infection of ∼1. RNA was collected from the infected and mock-infected cells after 2 days. The mRNA was enriched, reverse transcribed, and subjected to RNA sequencing. Relative mRNA levels were calculated according to the frequency per kilobase of exon per million read values after normalization by the values of mock-infected sample. KMT, lysine methyltransferase; KDM, lysine demethylase. The RNA-seq data are available at the DDBJ Sequence Read Archive (accession ID DRA006767 ). (B and C) Peripheral B cells from different donors were infected with EBV as in panel A and analyzed by qRT-PCR. Relative EZH2 mRNA levels are shown after normalization with beta-2 microglobulin (B2M). Average and SD from three independent infections are shown. Student’s t test was performed. (D and E) Akata(−) cells were infected with EBV as in panel A and analyzed by qRT-PCR. Relative EZH1 and EZH2 mRNA levels are shown after normalization with beta-2 microglobulin (B2M). Average and SD from three independent infections are shown. Student’s t test was performed. *, P
Techniques Used: Infection, Isolation, RNA Sequencing Assay, Sequencing, Quantitative RT-PCR
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