Structured Review

Medicago faba bean
Frequency histograms showing the distribution of number of <t>contigs</t> versus function of read length (A, B)/no. of reads (C, D) in field pea and <t>faba</t> bean, respectively .
Faba Bean, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers"

Article Title: Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers

Journal: BMC Genomics

doi: 10.1186/1471-2164-13-104

Frequency histograms showing the distribution of number of contigs versus function of read length (A, B)/no. of reads (C, D) in field pea and faba bean, respectively .
Figure Legend Snippet: Frequency histograms showing the distribution of number of contigs versus function of read length (A, B)/no. of reads (C, D) in field pea and faba bean, respectively .

Techniques Used:

2) Product Images from "Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)"

Article Title: Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)

Journal: Breeding Science

doi: 10.1270/jsbbs.64.252

Graphical view of syntenic relationship between A) faba bean and M. truncatula , and B) faba bean and L. japonicus . Syntenic regions between the two species are connected by colored lines. The colors of the lines represent LGs on the ‘Nubaria 2’ × ‘Misr 3’ map. Scales represent genetic position on LGs (cM, faba bean) and physical position on chromosomes (Mbp, M. truncatula and L. japonicus ).
Figure Legend Snippet: Graphical view of syntenic relationship between A) faba bean and M. truncatula , and B) faba bean and L. japonicus . Syntenic regions between the two species are connected by colored lines. The colors of the lines represent LGs on the ‘Nubaria 2’ × ‘Misr 3’ map. Scales represent genetic position on LGs (cM, faba bean) and physical position on chromosomes (Mbp, M. truncatula and L. japonicus ).

Techniques Used:

3) Product Images from "The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies"

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Journal: Journal of Virology

doi: 10.1128/JVI.00561-17

Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.
Figure Legend Snippet: Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.

Techniques Used: Amplification

Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
Figure Legend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

Techniques Used: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

4) Product Images from "The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies"

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Journal: Journal of Virology

doi: 10.1128/JVI.00561-17

Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.
Figure Legend Snippet: Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.

Techniques Used: Amplification

Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
Figure Legend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

Techniques Used: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

5) Product Images from "Traits affecting early season nitrogen uptake in nine legume species"

Article Title: Traits affecting early season nitrogen uptake in nine legume species

Journal: Heliyon

doi: 10.1016/j.heliyon.2017.e00244

(a) Factor loadings for the variables measured on nine legume species grown under high soil N availability for the first two axes of the principal component (PC) analysis. The percentage of the total variance explained by the first two principal components is shown in parentheses. (b) Projection of the different species on the first two axes of the principal component analysis. Variables: Shoot dry matter (Sdm), root dry matter (Rdm), total dry matter (Tdm), root:shoot ratio (R.S), initial seed dry matter (isdm), initial seed N (isN), N content in seeds (%sN), seed reserve depletion (srd), root lateral expansion rate (Rlr), root depth penetration rate (Rdr), nodule number (Nnb), nodule dry matter (Ndm), plant N (N), percentage of plant N derived from seeds (%Ns), Soil N uptake (Nso), N content in shoot dry matter (%NSdm), N content in root dry matter (%Nrdm), remaining N-NO 3 − in soil (NO 3 − ). Species: Alfalfa (Al), Fenugreek (Fe), Lentil (Le), Common vetch (Cv), Soybean (So), Pea (Pe), Chickpea (Cp), Peanut (Pn) and Faba bean (Fb).
Figure Legend Snippet: (a) Factor loadings for the variables measured on nine legume species grown under high soil N availability for the first two axes of the principal component (PC) analysis. The percentage of the total variance explained by the first two principal components is shown in parentheses. (b) Projection of the different species on the first two axes of the principal component analysis. Variables: Shoot dry matter (Sdm), root dry matter (Rdm), total dry matter (Tdm), root:shoot ratio (R.S), initial seed dry matter (isdm), initial seed N (isN), N content in seeds (%sN), seed reserve depletion (srd), root lateral expansion rate (Rlr), root depth penetration rate (Rdr), nodule number (Nnb), nodule dry matter (Ndm), plant N (N), percentage of plant N derived from seeds (%Ns), Soil N uptake (Nso), N content in shoot dry matter (%NSdm), N content in root dry matter (%Nrdm), remaining N-NO 3 − in soil (NO 3 − ). Species: Alfalfa (Al), Fenugreek (Fe), Lentil (Le), Common vetch (Cv), Soybean (So), Pea (Pe), Chickpea (Cp), Peanut (Pn) and Faba bean (Fb).

Techniques Used: Derivative Assay

(a) Factor loadings for the variables measured on nine legume species grown under low soil N availability, for the first two axes of the principal component (PC) analysis. The percentage of the total variance explained by the first two principal components is shown in parentheses. (b) Projections of the different species on the first two axes of the principal component analysis. Variables: Shoot dry matter (Sdm), root dry matter (Rdm), total dry matter (Tdm), root:shoot ratio (R.S), initial seed dry matter (isdm), initial seed N (isN), N content in seeds (%sN), seed reserve depletion (srd), root lateral expansion rate (Rlr), root depth penetration rate (Rdr), nodule number (Nnb), nodule dry matter (Ndm), plant N (N), percentage of plant N derived from seeds (%Ns), N 2 fixed (N 2 ), percentage of exogenous N derived from air (%Ndfa), soil N uptake (Nso), N content in shoot dry matter (%NSdm), N content in root dry matter (%Nrdm), remaining N-NO 3 − in soil (NO 3 − ). Species: Alfalfa (Al), Fenugreek (Fe), Lentil (Le), Common vetch (Cv), Soybean (So), Pea (Pe), Chickpea (Cp), Peanut (Pn) and Faba bean (Fb).
Figure Legend Snippet: (a) Factor loadings for the variables measured on nine legume species grown under low soil N availability, for the first two axes of the principal component (PC) analysis. The percentage of the total variance explained by the first two principal components is shown in parentheses. (b) Projections of the different species on the first two axes of the principal component analysis. Variables: Shoot dry matter (Sdm), root dry matter (Rdm), total dry matter (Tdm), root:shoot ratio (R.S), initial seed dry matter (isdm), initial seed N (isN), N content in seeds (%sN), seed reserve depletion (srd), root lateral expansion rate (Rlr), root depth penetration rate (Rdr), nodule number (Nnb), nodule dry matter (Ndm), plant N (N), percentage of plant N derived from seeds (%Ns), N 2 fixed (N 2 ), percentage of exogenous N derived from air (%Ndfa), soil N uptake (Nso), N content in shoot dry matter (%NSdm), N content in root dry matter (%Nrdm), remaining N-NO 3 − in soil (NO 3 − ). Species: Alfalfa (Al), Fenugreek (Fe), Lentil (Le), Common vetch (Cv), Soybean (So), Pea (Pe), Chickpea (Cp), Peanut (Pn) and Faba bean (Fb).

Techniques Used: Derivative Assay

Dry matter (a) and root:shoot ratio (b), root depth penetration (c) and lateral root expansion (d), nodule number (e) nodule dry matter (f), plant N (g), soil N uptake (h) with (N) and without N supply (0N) for nine legume species: Alfalfa (Al), Fenugreek (Fe), Lentil (Le), Common vetch (Cv), Soybean (So), Pea (Pe), Chickpea (Cp), Peanut (Pn) and Faba bean (Fb). ***, **, * indicate significant differences between the two levels of soil N availability at p
Figure Legend Snippet: Dry matter (a) and root:shoot ratio (b), root depth penetration (c) and lateral root expansion (d), nodule number (e) nodule dry matter (f), plant N (g), soil N uptake (h) with (N) and without N supply (0N) for nine legume species: Alfalfa (Al), Fenugreek (Fe), Lentil (Le), Common vetch (Cv), Soybean (So), Pea (Pe), Chickpea (Cp), Peanut (Pn) and Faba bean (Fb). ***, **, * indicate significant differences between the two levels of soil N availability at p

Techniques Used:

6) Product Images from "The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies"

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Journal: Journal of Virology

doi: 10.1128/JVI.00561-17

Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.
Figure Legend Snippet: Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.

Techniques Used: Amplification

Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
Figure Legend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

Techniques Used: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

7) Product Images from "The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies"

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Journal: Journal of Virology

doi: 10.1128/JVI.00561-17

Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.
Figure Legend Snippet: Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.

Techniques Used: Amplification

Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
Figure Legend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

Techniques Used: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

8) Product Images from "The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies"

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Journal: Journal of Virology

doi: 10.1128/JVI.00561-17

Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.
Figure Legend Snippet: Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.

Techniques Used: Amplification

Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
Figure Legend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

Techniques Used: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

9) Product Images from "The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies"

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

Journal: Journal of Virology

doi: 10.1128/JVI.00561-17

Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.
Figure Legend Snippet: Rolling-circle amplification rates for the different segments of FBNSV with DNA samples from faba bean (A) or Medicago (B) plants. Box plots show the distribution of RCA rates observed for 20 independent plants. Letters on top of the box plots determine groups of values not statistically different after a Tukey HSD test was performed on these data.

Techniques Used: Amplification

Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
Figure Legend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

Techniques Used: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

Related Articles

Acetylene Reduction Assay:

Article Title: The Unique Biosynthetic Route from Lupinus ?-Conglutin Gene to Blad
Article Snippet: .. This sequence, published under the NTrEMBL accession number Q6EBC1/GenBank accession number AY500372, was compared with the vicilin sequences of Vicia faba (vicilin precursor, CAA68525), Lens culinaris (allergen Len c, CAD87731), Pisum sativum (vicilin, CAA32239), Vicia narbonensis (vicilin precursor, CAA96514), Glycine max (alpha' subunit of beta-conglycinin, BAE02726), Phaseolus vulgaris (phaseolin, AAC04316), Canavalia gladiata (canavalin, CAA33172), Medicago truncatula (cupin, ABD28364), Juglans nigra (vicilin, AAM54366) and Arachis hypogaea (allergen Ara h 1, P43237). ..

Amplification:

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies
Article Snippet: .. More surprisingly, it also shows that the genome formula was distorted during RCA amplification (segment*RCA factor F value = 67.6; P < 2.2e−16 ) and that the effect of RCA was different in faba bean and Medicago (segment*plant*RCA factor F value = 11.2; P = 2.04e−13 ). .. While segment U4 experienced the highest relative proportion increase (26.5% before and 47% after RCA, i.e., a 1.77-fold increase, in faba bean, and 12.4% before and 17.8% after RCA, i.e., a 1.43-fold increase, in Medicago ), segment U2 suffered the highest relative proportion decrease (18.4% before and 4.6% after RCA, i.e., a 4-fold decrease, in faba bean, and 21.8% before and 12.3% after RCA, i.e., a 1.77-fold decrease, in Medicago ).

Next-Generation Sequencing:

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies
Article Snippet: .. It also shows that the genome formulae estimated by qPCR and by HiSeq are significantly different (segment*NGS factor F value = 14.4; P < 2.2e−16 ) but that the distortions induced by the NGS estimates are not statistically different in samples from faba beans and Medicago (segment*plant*RCA factor F value = 1.1; P = 0.37). .. Comparison of the average genome formulae in RCA products estimated by qPCR and by HiSeq ( and ) confirmed a quantitative difference with the HiSeq method.

Real-time Polymerase Chain Reaction:

Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies
Article Snippet: .. It also shows that the genome formulae estimated by qPCR and by HiSeq are significantly different (segment*NGS factor F value = 14.4; P < 2.2e−16 ) but that the distortions induced by the NGS estimates are not statistically different in samples from faba beans and Medicago (segment*plant*RCA factor F value = 1.1; P = 0.37). .. Comparison of the average genome formulae in RCA products estimated by qPCR and by HiSeq ( and ) confirmed a quantitative difference with the HiSeq method.

other:

Article Title: Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers
Article Snippet: Gene annotation Since M. truncatula is the model legume species that is most closely related to field pea and faba bean, consensus sequences from all contigs and singletons were preferentially compared to Medicago coding sequences.

Sequencing:

Article Title: Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)
Article Snippet: .. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula . ..

Article Title: The Unique Biosynthetic Route from Lupinus ?-Conglutin Gene to Blad
Article Snippet: .. This sequence, published under the NTrEMBL accession number Q6EBC1/GenBank accession number AY500372, was compared with the vicilin sequences of Vicia faba (vicilin precursor, CAA68525), Lens culinaris (allergen Len c, CAD87731), Pisum sativum (vicilin, CAA32239), Vicia narbonensis (vicilin precursor, CAA96514), Glycine max (alpha' subunit of beta-conglycinin, BAE02726), Phaseolus vulgaris (phaseolin, AAC04316), Canavalia gladiata (canavalin, CAA33172), Medicago truncatula (cupin, ABD28364), Juglans nigra (vicilin, AAM54366) and Arachis hypogaea (allergen Ara h 1, P43237). ..

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    Medicago faba bean plants
    Overview of the protocol for experiment 1. (A) <t>Faba</t> bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. <t>pisum</t> aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.
    Faba Bean Plants, supplied by Medicago, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/faba bean plants/product/Medicago
    Average 91 stars, based on 3 article reviews
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    Medicago vicia faba vf
    Protein alignment of forisomes proteins and distribution of tryparedoxin-like (TryX-like) family domain. (A) Amino acid alignment of forisomes proteins (For1) from Cannavalia gladiata (Cg), Medicago truncatula ( Mt ), <t>Vicia</t> <t>faba</t> (Vf) with sieve element
    Vicia Faba Vf, supplied by Medicago, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

    Journal: Journal of Virology

    Article Title: The Number of Target Molecules of the Amplification Step Limits Accuracy and Sensitivity in Ultradeep-Sequencing Viral Population Studies

    doi: 10.1128/JVI.00561-17

    Figure Lengend Snippet: Overview of the protocol for experiment 1. (A) Faba bean plants ( V. faba ) were agroinoculated with a mix of A. tumefaciens cultures carrying plasmids containing the FBNSV segments. Ten A. pisum aphids were used as vectors to transmit FBNSV from symptomatic faba bean plants to Medicago truncatula plants. Total DNA was collected from faba bean and Medicago plants. This procedure was performed on 20 independent lines. Vf1, Vicia faba ; Mt, Medicago truncatula . (B) Rolling-circle amplifications were performed on each of the 40 DNA samples collected from faba bean and Medicago plants (from the 20 lines described above for panel A). DNA purification was performed after RCA in order to remove the RCA buffer from the samples. Cleaned DNA samples were sent for sequencing. Genome formulae were measured by qPCR on pre- and post-RCA samples and estimated by counting the number of reads per segment after NGS analysis.

    Article Snippet: Aphid transmission of the virus was performed by caging 10 A. pisum aphids on infected faba bean plants for 3 days and then transferring them to 15-day-old Medicago plants ( Medicago truncatula ) for three more days.

    Techniques: DNA Purification, Sequencing, Real-time Polymerase Chain Reaction, Next-Generation Sequencing

    Dot-plots for synteny and collinearity comparisons between the faba bean consensus map (cM) and the genomes (bp) of ( A ) Pisum sativum ; ( B ) Medicago truncatula ; ( C ) Cicer arietinum ; ( D ) Vigna unguiculata ( E ) Phaseolus vulgaris; ( F ) Glycine max and ( G ) Lotus japonicas .

    Journal: Scientific Reports

    Article Title: Development of new genetic resources for faba bean (Vicia faba L.) breeding through the discovery of gene-based SNP markers and the construction of a high-density consensus map

    doi: 10.1038/s41598-020-63664-7

    Figure Lengend Snippet: Dot-plots for synteny and collinearity comparisons between the faba bean consensus map (cM) and the genomes (bp) of ( A ) Pisum sativum ; ( B ) Medicago truncatula ; ( C ) Cicer arietinum ; ( D ) Vigna unguiculata ( E ) Phaseolus vulgaris; ( F ) Glycine max and ( G ) Lotus japonicas .

    Article Snippet: In fact, the SNPs used to construct the consensus map of Webb et al . were designed based on orthologous sequences in M. truncatula with the objective of physically anchoring the faba bean consensus map to the Medicago genome.

    Techniques:

    Protein alignment of forisomes proteins and distribution of tryparedoxin-like (TryX-like) family domain. (A) Amino acid alignment of forisomes proteins (For1) from Cannavalia gladiata (Cg), Medicago truncatula ( Mt ), Vicia faba (Vf) with sieve element

    Journal: Plant Signaling & Behavior

    Article Title: Conserved thioredoxin fold is present in Pisum sativum L. sieve element occlusion-1 protein

    doi:

    Figure Lengend Snippet: Protein alignment of forisomes proteins and distribution of tryparedoxin-like (TryX-like) family domain. (A) Amino acid alignment of forisomes proteins (For1) from Cannavalia gladiata (Cg), Medicago truncatula ( Mt ), Vicia faba (Vf) with sieve element

    Article Snippet: The amino acid sequences of forisomes proteins (For1) from Cannavalia gladiata (Cg), Medicago truncatula ( Mt ), Vicia faba (Vf), and the sieve element occlusion (SEO) proteins (Mt.SEO1, 2 and 3) of Medicago truncatula (Mt), Pisum sativum ( Ps.SEO1 ) were downloaded from NCBI web server ( ).

    Techniques:

    lncRNA interspecies conservation and genome alignment analysis: the graph shows the total number of genomes versus the total number of transcripts aligned to Fabaceae genomes ( Vicia faba , Glycine max , Medicago truncatula , Phaseolus vulgaris , Lotus japonica , Vigna unguiculata and Cicer reticulatum ). The blue line represents the lncRNA gathered from the ARF samples and the red line represents the lncRNA gathered from the CER sample, their alignment profile is very similar as expected, around 1800 transcripts aligned to at least one of the genomes used.

    Journal: Non-Coding RNA

    Article Title: Copaifera langsdorffii Novel Putative Long Non-Coding RNAs: Interspecies Conservation Analysis in Adaptive Response to Different Biomes

    doi: 10.3390/ncrna4040027

    Figure Lengend Snippet: lncRNA interspecies conservation and genome alignment analysis: the graph shows the total number of genomes versus the total number of transcripts aligned to Fabaceae genomes ( Vicia faba , Glycine max , Medicago truncatula , Phaseolus vulgaris , Lotus japonica , Vigna unguiculata and Cicer reticulatum ). The blue line represents the lncRNA gathered from the ARF samples and the red line represents the lncRNA gathered from the CER sample, their alignment profile is very similar as expected, around 1800 transcripts aligned to at least one of the genomes used.

    Article Snippet: Since there is no reference genome for C . langsdorffii , we performed a similarity search using Bowtie2 (v2.3.4.1) [ ] against the genomes of the following related species: (i) Vicia faba , (ii) Glycine max [ ], (iii) Medicago truncatula [ ], (iv) Phaseolus vulgaris [ ], (v) Lotus japonica [ ], (vi) Vigna unguiculata [ ] and (vii) Cicer reticulatum [ ].

    Techniques: