f4 80 staining required proteinase k  (Millipore)


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    Structured Review

    Millipore f4 80 staining required proteinase k
    F4 80 Staining Required Proteinase K, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f4 80 staining required proteinase k/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f4 80 staining required proteinase k - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    pronase
    ucp1 staining

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: For immunohistochemistry, sections were deparaffinized and antigen retrieval was done by incubation with hot citrate buffer. .. In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C.

    Avidin-Biotin Assay:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C. .. The avidin-biotin complex was detected with a 3-amino-9-ethylcarbazole (AEC) peroxidase substrate kit (Vector Laboratories).

    Incubation:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: .. In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C. .. Sections were permeabilized with 0.4% Triton X-100 and blocked with serum from an ABC kit (Vector Laboratories, Peterborough, United Kingdom).

    Inhibition:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: Inhibition of intrinsic peroxidase activity was performed with 0.5% H2 O2 treatment. .. In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C.

    Activity Assay:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: Inhibition of intrinsic peroxidase activity was performed with 0.5% H2 O2 treatment. .. In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C.

    Staining:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: .. In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C. .. Sections were permeabilized with 0.4% Triton X-100 and blocked with serum from an ABC kit (Vector Laboratories, Peterborough, United Kingdom).

    Plasmid Preparation:

    Article Title: Adipocyte-Specific Hypoxia-Inducible Factor 2α Deficiency Exacerbates Obesity-Induced Brown Adipose Tissue Dysfunction and Metabolic Dysregulation
    Article Snippet: In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C. .. In addition, F4/80 staining required proteinase K (Sigma-Aldrich) treatment at 37°C, whereas for UCP1 staining, sections were also incubated with pronase (Sigma-Aldrich) at 37°C.

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  • 99
    Millipore anti f4 80
    Hepatic LRP1 deficiency promotes monocyte-macrophage infiltration into the liver. The hLrp1 +/+ and hLrp1 −/− mice were fed the HFHC diet for 16 weeks. Liver sections were prepared from these animals for immunofluorescence detection of <t>F4/80</t> ( top panels ) and CD68 + cells ( middle panels ). The number of F4/80 + and CD68 + cells in the livers of hLrp1 +/+ ( filled bars ) and hLrp1 −/− ( open bars ) mice were also quantified by flow cytometry. The data represent mean ± S.E. *, significant difference from hLrp1 +/+ mice at p
    Anti F4 80, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti f4 80/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti f4 80 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    97
    Millipore f4 80
    Effect of exendin-4 on myocardial inflammation after MI. Representative LV sections stained for a – e CD45, with positive spleen control, and g – j <t>F4/80,</t> to assess leukocyte and macrophage infiltration, respectively. f , k Quantification data ( n = 5–6). White columns sham; black columns MI; mean ± SEM. *** P
    F4 80, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f4 80/product/Millipore
    Average 97 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    f4 80 - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    85
    Millipore anti f4 80 antigen
    The differential degeneration of DA neurons is not attributable to different inflammatory reactions. A , LPS-induced activation of microglia in the mouse SN. One week after the injection, brain sections were immunostained with an antibody against <t>F4/80</t> to determine the activation status of the microglia. In the NS-injected SN, microglia exhibited the typical ramified morphology of resting microglia. In the LPS-injected SN, microglia were highly activated with the characteristics of larger size, irregular shape, and dramatically increased expression of the F4/80 antigen. Note that the activation status of microglia appeared similar in all genotypes. B , Western blot analysis showed strikingly increased expression of F4/80 in the mouse midbrain 1 week after LPS injection. The levels of F4/80 expression were not different among different mouse genotypes. C , Midbrain neuron-enriched cultures from SYNKO or M7KO mice were supplemented with 5 × 10 4 microglia per well prepared from either SYNKO or M7KO mice. After 24 h, the cultures were treated with NS or 10 ng/ml LPS, and [ 3 H]DAuptakewasdetermined7dafterthetreatment. p
    Anti F4 80 Antigen, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti f4 80 antigen/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti f4 80 antigen - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Hepatic LRP1 deficiency promotes monocyte-macrophage infiltration into the liver. The hLrp1 +/+ and hLrp1 −/− mice were fed the HFHC diet for 16 weeks. Liver sections were prepared from these animals for immunofluorescence detection of F4/80 ( top panels ) and CD68 + cells ( middle panels ). The number of F4/80 + and CD68 + cells in the livers of hLrp1 +/+ ( filled bars ) and hLrp1 −/− ( open bars ) mice were also quantified by flow cytometry. The data represent mean ± S.E. *, significant difference from hLrp1 +/+ mice at p

    Journal: The Journal of Biological Chemistry

    Article Title: Low-density lipoprotein receptor–related protein-1 dysfunction synergizes with dietary cholesterol to accelerate steatohepatitis progression

    doi: 10.1074/jbc.RA118.001952

    Figure Lengend Snippet: Hepatic LRP1 deficiency promotes monocyte-macrophage infiltration into the liver. The hLrp1 +/+ and hLrp1 −/− mice were fed the HFHC diet for 16 weeks. Liver sections were prepared from these animals for immunofluorescence detection of F4/80 ( top panels ) and CD68 + cells ( middle panels ). The number of F4/80 + and CD68 + cells in the livers of hLrp1 +/+ ( filled bars ) and hLrp1 −/− ( open bars ) mice were also quantified by flow cytometry. The data represent mean ± S.E. *, significant difference from hLrp1 +/+ mice at p

    Article Snippet: The cells were incubated with conjugated anti-F4/80 or anti-CD68 with secondary antibodies conjugated to Alexa Fluor 488 and then analyzed using the Guava EasyCyteTM 8HT flow cytometry system (Millipore).

    Techniques: Mouse Assay, Immunofluorescence, Flow Cytometry, Cytometry

    Effect of exendin-4 on myocardial inflammation after MI. Representative LV sections stained for a – e CD45, with positive spleen control, and g – j F4/80, to assess leukocyte and macrophage infiltration, respectively. f , k Quantification data ( n = 5–6). White columns sham; black columns MI; mean ± SEM. *** P

    Journal: Basic Research in Cardiology

    Article Title: Exendin-4 protects against post-myocardial infarction remodelling via specific actions on inflammation and the extracellular matrix

    doi: 10.1007/s00395-015-0476-7

    Figure Lengend Snippet: Effect of exendin-4 on myocardial inflammation after MI. Representative LV sections stained for a – e CD45, with positive spleen control, and g – j F4/80, to assess leukocyte and macrophage infiltration, respectively. f , k Quantification data ( n = 5–6). White columns sham; black columns MI; mean ± SEM. *** P

    Article Snippet: Immunohistochemistry for CD45 and F4/80 was performed with rabbit polyclonal (Millipore), rat polyclonal (BD Biosciences), and rat monoclonal (Abcam) antibodies (1:1000), respectively, using diaminobenzidine as the chromogen and nuclear counterstaining with haematoxylin.

    Techniques: Staining

    Mice lacking Nrp1 in microglia/macrophages and mice treated with an Nrp1 inhibitor exhibit impaired glioma growth A. Representative images of the GL261-eGFP- gliomas and CD31 + tumor vasculature in wild-type (wt) mice (top), Nrp1 MgKO mice (middle), and wt mice administered the Nrp1 inhibitor EG00229 (75μM) via mini-osmotic pump/cannula (bottom) at 15 days post-glioma induction (PGI). B. Representative staining of Csf1R in tumors from wt and Nrp1 MgKO mice. C. Representative staining of Nrp1 by F4/80+ GAMs in wt and Nrp1 MgKO mice. D. Quantification of tumor areas, and E. quantification of vessel luminal areas bordered by CD31 + staining in gliomas in wt, Nrp1 MgKO , and EG00229-treated mice at 15 and 20 days PGI. Mice were administered EG00229 either throughout the disease course or after day 8 (D8, **= p

    Journal: Oncotarget

    Article Title: Ablation of Neuropilin 1 from glioma-associated microglia and macrophages slows tumor progression

    doi: 10.18632/oncotarget.6877

    Figure Lengend Snippet: Mice lacking Nrp1 in microglia/macrophages and mice treated with an Nrp1 inhibitor exhibit impaired glioma growth A. Representative images of the GL261-eGFP- gliomas and CD31 + tumor vasculature in wild-type (wt) mice (top), Nrp1 MgKO mice (middle), and wt mice administered the Nrp1 inhibitor EG00229 (75μM) via mini-osmotic pump/cannula (bottom) at 15 days post-glioma induction (PGI). B. Representative staining of Csf1R in tumors from wt and Nrp1 MgKO mice. C. Representative staining of Nrp1 by F4/80+ GAMs in wt and Nrp1 MgKO mice. D. Quantification of tumor areas, and E. quantification of vessel luminal areas bordered by CD31 + staining in gliomas in wt, Nrp1 MgKO , and EG00229-treated mice at 15 and 20 days PGI. Mice were administered EG00229 either throughout the disease course or after day 8 (D8, **= p

    Article Snippet: Co-staining with iba1 or F4/80 and CD86 (1:50) (Millipore), 1:50 CD206 (R & D Systems Inc.), and 1:500 TSPO (Abcam) was used to evaluate GAM polarization.

    Techniques: Mouse Assay, Staining

    The differential degeneration of DA neurons is not attributable to different inflammatory reactions. A , LPS-induced activation of microglia in the mouse SN. One week after the injection, brain sections were immunostained with an antibody against F4/80 to determine the activation status of the microglia. In the NS-injected SN, microglia exhibited the typical ramified morphology of resting microglia. In the LPS-injected SN, microglia were highly activated with the characteristics of larger size, irregular shape, and dramatically increased expression of the F4/80 antigen. Note that the activation status of microglia appeared similar in all genotypes. B , Western blot analysis showed strikingly increased expression of F4/80 in the mouse midbrain 1 week after LPS injection. The levels of F4/80 expression were not different among different mouse genotypes. C , Midbrain neuron-enriched cultures from SYNKO or M7KO mice were supplemented with 5 × 10 4 microglia per well prepared from either SYNKO or M7KO mice. After 24 h, the cultures were treated with NS or 10 ng/ml LPS, and [ 3 H]DAuptakewasdetermined7dafterthetreatment. p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Neuroinflammation and Oxidation/Nitration of ?-Synuclein Linked to Dopaminergic Neurodegeneration

    doi: 10.1523/JNEUROSCI.0143-07.2008

    Figure Lengend Snippet: The differential degeneration of DA neurons is not attributable to different inflammatory reactions. A , LPS-induced activation of microglia in the mouse SN. One week after the injection, brain sections were immunostained with an antibody against F4/80 to determine the activation status of the microglia. In the NS-injected SN, microglia exhibited the typical ramified morphology of resting microglia. In the LPS-injected SN, microglia were highly activated with the characteristics of larger size, irregular shape, and dramatically increased expression of the F4/80 antigen. Note that the activation status of microglia appeared similar in all genotypes. B , Western blot analysis showed strikingly increased expression of F4/80 in the mouse midbrain 1 week after LPS injection. The levels of F4/80 expression were not different among different mouse genotypes. C , Midbrain neuron-enriched cultures from SYNKO or M7KO mice were supplemented with 5 × 10 4 microglia per well prepared from either SYNKO or M7KO mice. After 24 h, the cultures were treated with NS or 10 ng/ml LPS, and [ 3 H]DAuptakewasdetermined7dafterthetreatment. p

    Article Snippet: Immunostaining was performed with the following primary antibodies: anti-F4/80 antigen, anti-glial fibrillary acidic protein antiserum, antibodies against a neuron-specific nuclear protein (NeuN) and tyrosine hydroxylase (TH) , anti-synaptophysin antibody (1:5000; Millipore), LB509, SYN211 and SYN505 (1:500; specific to human SYN), affinity-purified SNL1 (1:1200; recognizing both human and mouse SYN), nSYN14 (specific to nitrated α- and β-synuclein) , and anti-SYN phosphorylated at Ser129 ( ).

    Techniques: Activation Assay, Injection, Expressing, Western Blot, Mouse Assay