sciflexarrayer s3 piezo electric dispenser  (SCIENION)

 
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    Name:
    sciFLEXARRAYER S3
    Description:
    Picoliter precsion liquid handler
    Catalog Number:
    f003
    Price:
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    Category:
    Arrayer
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    SCIENION sciflexarrayer s3 piezo electric dispenser
    Picoliter precsion liquid handler
    https://www.bioz.com/result/sciflexarrayer s3 piezo electric dispenser/product/SCIENION
    Average 95 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    sciflexarrayer s3 piezo electric dispenser - by Bioz Stars, 2020-02
    95/100 stars

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    Diagnostic Assay:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: Typically, biomarker analytes are vastly outnumbered by endogenous proteins and macromolecules found in unprocessed patient samples, ensuring that the diagnostic assay can specifically and sensitively detect the target within a complex solution, which is imperative. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Blocking Assay:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody. .. After an overnight incubation, the surface was first treated with 50-mM ethanolamine (pH 8), then washed with phosphate buffered saline (PBS) containing 0.1% tween-20 to remove any remaining unbound material followed by blocking for 1 h in a petri dish with 1% bovine serum albumin with PBS.

    Microarray:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: Paragraph title: Microarray preparation ... The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany).

    Article Title: Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
    Article Snippet: .. The mucins were printed onto Nexterion® slide H microarray slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany). ..

    Article Title: Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Article Snippet: .. Before the printing of the protein microarray with a sciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) the print media need to be prepared. ..

    Article Title: Microarray screening of Guillain-Barré syndrome sera for antibodies to glycolipid complexes
    Article Snippet: .. Glycolipids microarray slides were produced using a Sciflexarrayer S3 microarray printer (Scienion; Berlin, Germany). ..

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: .. The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion). .. Two rows, with five spots each, were printed with 20 nL per spot of 10 × 10−3 m MMP fluorogenic peptide substrate solution, and the other two rows, also printed with five spots each, were modified with 20 nL per spot of 1 × 10−3 m SrtA fluorogenic peptide substrate solution.

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Paragraph title: Protein Preparation and Microarray Printing ... Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer.

    Article Title: Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
    Article Snippet: Paragraph title: EW microarray construction ... Microarrays were printed with a SciFLEXARRAYER S3 (Scienion) piezoelectric printer equipped with a 90 μm glass nozzle with a hydrophobic coating.

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: .. A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ). .. Microarray incubations were carried out essentially as previously described ( ) and all procedures were carried out in the dark.

    Incubation:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. The chips were then incubated with 0, 200, 400, and 800 nM of purified gonococcal Fur protein , , , in 2 mL of binding buffer (20 mM Tris-Cl pH 7.9, 5 mM MgCl2 , 40 mM KCl, 0.125 mM MnCl2 , 2 mM DTT, 10% Glycerol, 0.19 µg/µL poly dIdC), respectively at RT for 2 hr.

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody. .. After an overnight incubation, the surface was first treated with 50-mM ethanolamine (pH 8), then washed with phosphate buffered saline (PBS) containing 0.1% tween-20 to remove any remaining unbound material followed by blocking for 1 h in a petri dish with 1% bovine serum albumin with PBS.

    Article Title: Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
    Article Snippet: The mucins were printed onto Nexterion® slide H microarray slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany). .. Slides were incubated in a humid atmosphere overnight and active functional groups were deactivated by incubated in 100 mM ethanolamine in 50 mM sodium borate, pH 8.0, for 1 hr.

    Article Title: Ultrasensitive interferometric on-chip microscopy of transparent objects
    Article Snippet: An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a sciFLEXARRAYER S3 spotter (Scienion; see ). .. After incubation with anti-BSA immunoglobulin G (IgG), which specifically binds to BSA, and rinsing, the BSA monolayer spots were converted to BSA + IgG bilayer spots.

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion). .. This array was incubated with human chronic wound fluid and bacterial culture medium before imaging using a confocal microscope.

    Article Title: Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays
    Article Snippet: Amino-functionalized capture probe DNA (5′-NH2-TTCAAAATTGCGAAGTTGGG-3′) was mixed with aldehyde dextran in 3X saline-sodium citrate buffer (SSC, pH 7.0, Sigma Aldrich, St. Louis, MO, USA) and incubated for 30 min at 30 °C. .. This solution was spotted on the APTES coated slide using a sciFLEXARRAYER S3 (Scienion, Berlin, Germany), a piezo-driven non-contact dispensing system, forming a line of five spots.

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ). .. In brief, FhTeg-555 was diluted in Tris-buffered saline supplemented with Ca2+ and Mg2+ ions (TBS; 20 mm Tris-HCl, 100 mm NaCl, 1 mm CaCl2, 1 mm MgCl2 , pH 7.2) and with 0.05% Tween 20 (TBS-T) for incubation on the microarrays using an eight-well gasket (Agilent Technologies, Cork, Ireland) at room temperature for 1 h with gentle rotation (4 rpm) in the dark.

    Modification:

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion). .. Two rows, with five spots each, were printed with 20 nL per spot of 10 × 10−3 m MMP fluorogenic peptide substrate solution, and the other two rows, also printed with five spots each, were modified with 20 nL per spot of 1 × 10−3 m SrtA fluorogenic peptide substrate solution.

    Protein Array:

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer. .. 30 autoantigens and 4 control proteins were spotted onto each subarray in triplicate, resulting in a 10×10 protein array within each subarray.

    High Performance Liquid Chromatography:

    Article Title: Unique Reporter-Based Sensor Platforms to Monitor Signalling in Cells
    Article Snippet: Microarrays Unmodified HPLC-purified oligonucleotide captures ( ) were diluted to 25 µM in 0.15 M NaH2 PO4 (pH 8.5) and 280 ρL used per spot. .. 8 subarrays were generated on each epoxy-coated glass slide using the Scienion SciFlexarrayer S3; each subarray included 5 replicates of each UR and Rluc.

    Conjugation Assay:

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: Profiling of FhTeg Oligosaccharides by Lectin Microarray FhTeg was fluorescently labeled with Alexa Fluor 555 via carboxylic acid succinimidyl ester conjugation strategy according to manufacturer instructions (ThermoFischer Scientific. .. A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ).

    Flow Cytometry:

    Article Title: Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays
    Article Snippet: The silanization was carried out by immersion in a 3% (v /v ) solution of 3-amino-propyl-triethoxy-silane (APTES) in 95% ethanol for 2 h. The substrates were rinsed with ethanol and water, dried under nitrogen flow, and cured for 1 h in an oven at 80 °C. .. This solution was spotted on the APTES coated slide using a sciFLEXARRAYER S3 (Scienion, Berlin, Germany), a piezo-driven non-contact dispensing system, forming a line of five spots.

    Biomarker Assay:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: Typically, biomarker analytes are vastly outnumbered by endogenous proteins and macromolecules found in unprocessed patient samples, ensuring that the diagnostic assay can specifically and sensitively detect the target within a complex solution, which is imperative. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Generated:

    Article Title: Unique Reporter-Based Sensor Platforms to Monitor Signalling in Cells
    Article Snippet: .. 8 subarrays were generated on each epoxy-coated glass slide using the Scienion SciFlexarrayer S3; each subarray included 5 replicates of each UR and Rluc. .. The fabricated slides were incubated overnight at ambient temperature in 70% relative humidity, pre-hybridised and washed using standard conditions.

    other:

    Article Title: Picoliter‐volume inkjet printing into planar microdevice reservoirs for low‐waste, high‐capacity drug loading
    Article Snippet: 2.4 Drug loading into microdevices Drug printing was performed using the sciFLEXARRAYER S3 (Scienion AG, Johannisthal, Germany) (Figure ).

    Article Title: Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection
    Article Snippet: Printing of Test and Control Lines The membrane was printed with a streptavidin test line and an anti-mouse antibody control line using a Scienion sciFLEXARRAYER S3 with a type 4 piezo.

    Imaging:

    Article Title: Ultrasensitive interferometric on-chip microscopy of transparent objects
    Article Snippet: The corresponding OPDs recovered from LIM imaging using the PSI method agreed with the AFM measurements. .. An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a sciFLEXARRAYER S3 spotter (Scienion; see ).

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion). .. This array was incubated with human chronic wound fluid and bacterial culture medium before imaging using a confocal microscope.

    Protein Concentration:

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: Final protein concentration and labeling efficiency was calculated according to the manufacturer's instructions by measuring sample absorbance at 280 and 550 nm and using the arbitrary values for molecular mass (100,000) and extinction coefficient (E of 10). .. A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ).

    Polymerase Chain Reaction:

    Article Title: Unique Reporter-Based Sensor Platforms to Monitor Signalling in Cells
    Article Snippet: 8 subarrays were generated on each epoxy-coated glass slide using the Scienion SciFlexarrayer S3; each subarray included 5 replicates of each UR and Rluc. .. Duplicate UR and Rluc PCR reactions were performed for each cDNA sample (3 µL) using Cy3/Cy5-labeled sense and unlabelled antisense primers (2 µM UR primers and 1 µM Rluc primers, ) for 25 cycles in a Mastercycler epgradient PCR machine (Eppendorf) using HotStarTaq DNA polymerase (Qiagen).

    Recombinant:

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Recombinant protein generation was outsourced to Origene (Rockville, MD), where protein production was performed in CHO cells and the proteins were purified to 80-90% purity. .. Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer.

    Molecular Weight:

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: Excess dye was removed from FhTeg-555 using a desalting column (7 kDa molecular weight cut off (MWCO) (Invitrogen, Paisley, UK)). .. A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ).

    Fluorescence:

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: The surface was illuminated with light of a wavelength of 495 nm to collect the fluorescence micrograph with an emission range of 510–560 nm. pSiRM Microarray : We also prepared a pSiRM array to immobilize two different fluorogenic peptide substrates. .. The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion).

    Negative Control:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: An antibody against the wtVSV surface glycoprotein (8G5 monoclonal antibody) and a negative control monoclonal antibody (specific for the Marburg virus glycoprotein) were arrayed onto the sensor described in Section B-I, which is functionalized with the MCP-2 co-polymer from Lucidant Polymers LLC (Sunnyvale, CA, USA). .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Article Title: Microarray screening of Guillain-Barré syndrome sera for antibodies to glycolipid complexes
    Article Snippet: Glycolipids microarray slides were produced using a Sciflexarrayer S3 microarray printer (Scienion; Berlin, Germany). .. All glycolipid targets were printed in duplicate on each array and included methanol solvent, which was printed as a negative control.

    Microscopy:

    Article Title: Microarray screening of Guillain-Barré syndrome sera for antibodies to glycolipid complexes
    Article Snippet: In brief, array platforms were produced in-house from polyvinylidene fluoride membrane adhered to glass microscope slides. .. Glycolipids microarray slides were produced using a Sciflexarrayer S3 microarray printer (Scienion; Berlin, Germany).

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: The surface was then observed using a Carl Zeiss LSM 710 confocal microscope with an EC Plan‐Neofluar 10×/0.30 M27 objective. .. The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion).

    Purification:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. The chips were then incubated with 0, 200, 400, and 800 nM of purified gonococcal Fur protein , , , in 2 mL of binding buffer (20 mM Tris-Cl pH 7.9, 5 mM MgCl2 , 40 mM KCl, 0.125 mM MnCl2 , 2 mM DTT, 10% Glycerol, 0.19 µg/µL poly dIdC), respectively at RT for 2 hr.

    Article Title: Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
    Article Snippet: Purified human gastric and colonic mucins ( ) were printed onto separate microarray slides as described previously [ ]. .. The mucins were printed onto Nexterion® slide H microarray slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany).

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Recombinant protein generation was outsourced to Origene (Rockville, MD), where protein production was performed in CHO cells and the proteins were purified to 80-90% purity. .. Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer.

    Article Title: Unique Reporter-Based Sensor Platforms to Monitor Signalling in Cells
    Article Snippet: 8 subarrays were generated on each epoxy-coated glass slide using the Scienion SciFlexarrayer S3; each subarray included 5 replicates of each UR and Rluc. .. The fluorescently-labelled amplicons were purified using the QIAquick PCR purification kit (Qiagen), diluted 4 fold with hybridization buffer, denatured for 5 minutes at 95°C, and 4 µL of the diluted amplicons were placed on two successive subarrays which were incubated overnight in a humidified chamber at 53°C.

    Labeling:

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: Final protein concentration and labeling efficiency was calculated according to the manufacturer's instructions by measuring sample absorbance at 280 and 550 nm and using the arbitrary values for molecular mass (100,000) and extinction coefficient (E of 10). .. A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ).

    Confocal Microscopy:

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: Biosensor Characterization Using Confocal Microscopy : Confocal microscopy was used to image the fluorescence emission of the pSiRM surface. .. The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion).

    Chromatin Immunoprecipitation:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. IRIS images were taken for each chip to determine the initial dsDNA densities corresponding to each spot in the microarray.

    Hybridization:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: Microarray preparation Amino-labeled forward DNA strands (50 µM) ( ) were mixed with excess complementary strands to create dsDNA through standard hybridization methods: mixed DNA solutions were heated to 80°C for 10 min and then allowed to cool to room temperature in a slow, controlled manner ( < 1°C/min). .. The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany).

    Article Title: Unique Reporter-Based Sensor Platforms to Monitor Signalling in Cells
    Article Snippet: 8 subarrays were generated on each epoxy-coated glass slide using the Scienion SciFlexarrayer S3; each subarray included 5 replicates of each UR and Rluc. .. The fluorescently-labelled amplicons were purified using the QIAquick PCR purification kit (Qiagen), diluted 4 fold with hybridization buffer, denatured for 5 minutes at 95°C, and 4 µL of the diluted amplicons were placed on two successive subarrays which were incubated overnight in a humidified chamber at 53°C.

    Functional Assay:

    Article Title: Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
    Article Snippet: The mucins were printed onto Nexterion® slide H microarray slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany). .. Slides were incubated in a humid atmosphere overnight and active functional groups were deactivated by incubated in 100 mM ethanolamine in 50 mM sodium borate, pH 8.0, for 1 hr.

    Binding Assay:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. The chips were then incubated with 0, 200, 400, and 800 nM of purified gonococcal Fur protein , , , in 2 mL of binding buffer (20 mM Tris-Cl pH 7.9, 5 mM MgCl2 , 40 mM KCl, 0.125 mM MnCl2 , 2 mM DTT, 10% Glycerol, 0.19 µg/µL poly dIdC), respectively at RT for 2 hr.

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: The presence of other macro-molecules can lead to nonspecific binding which decreases the test confidence. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Article Title: Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities). Toward Multiplexing Detection of Wound Healing Biomarkers on Porous Silicon Resonant Microcavities
    Article Snippet: A PDMS mask was used to protect half of the hydrosilylated pSiRM surface during covalent binding of the SrtA peptide substrate. .. The activated pSiRM surface was printed using a SciFlexarrayer – S3 Microarray (Scienion).

    Article Title: Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays
    Article Snippet: Paragraph title: 2.2. Binding Assay ... This solution was spotted on the APTES coated slide using a sciFLEXARRAYER S3 (Scienion, Berlin, Germany), a piezo-driven non-contact dispensing system, forming a line of five spots.

    Produced:

    Article Title: Microarray screening of Guillain-Barré syndrome sera for antibodies to glycolipid complexes
    Article Snippet: .. Glycolipids microarray slides were produced using a Sciflexarrayer S3 microarray printer (Scienion; Berlin, Germany). ..

    Concentration Assay:

    Article Title: Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
    Article Snippet: Briefly, the mucins were dissolved in phosphate buffered saline, pH 7.4, (PBS) to a final concentration of 0.5 mg/mL in PBS with or without a range of concentrations of detergent ( ). .. The mucins were printed onto Nexterion® slide H microarray slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany).

    Article Title: Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Article Snippet: Before the printing of the protein microarray with a sciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) the print media need to be prepared. .. Therefore, the lyophilised copolymer was dissolved in deionized water at a concentration of 10 mg/ml.

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ). .. Initially several concentrations (2.9–14.4 μg/ml) were titrated to determine the best concentration for optimal signal to noise ratio.

    Construct:

    Article Title: Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
    Article Snippet: Construction of Animal and Human Gastrointestinal Tract (GIT) Mucin Microarrays Microarrays containing 38 purified mucins from the GITs of eight different animal species and two mucins from colonic cell lines, E12 [ ] and LS174T [ ], ( ) were constructed as previously described [ ]. .. The mucins were printed onto Nexterion® slide H microarray slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany).

    Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *
    Article Snippet: .. A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ). .. Microarray incubations were carried out essentially as previously described ( ) and all procedures were carried out in the dark.

    Transmission Electron Microscopy:

    Article Title: Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles
    Article Snippet: The Scienion SciFLEXARRAYER S3 (See ) is a commercial, fully programmable, computer controlled liquid handling system that transfers specific picoliter sample volumes utilizing Piezo Dispense Capillaries (PDCs). .. A computer controlled carriage moves a platform holding from 1 to 8 PDCs between targets, with a positional accuracy of < 5 μ m. Both the 40 μ m and the 60 μ m bore tips (100-300 pL range for single droplets) were used for spotting on TEM grids as described in this paper.

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    SCIENION sciflexarrayer s3 piezo electric dispenser
    Sciflexarrayer S3 Piezo Electric Dispenser, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sciflexarrayer s3 piezo electric dispenser/product/SCIENION
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    sciflexarrayer s3 piezo electric dispenser - by Bioz Stars, 2020-02
    95/100 stars
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