microspotter  (SCIENION)

 
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    Name:
    sciFLEXARRAYER S3
    Description:
    Picoliter precsion liquid handler
    Catalog Number:
    F003
    Price:
    None
    Category:
    Arrayer
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    Structured Review

    SCIENION microspotter
    Picoliter precsion liquid handler
    https://www.bioz.com/result/microspotter/product/SCIENION
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    microspotter - by Bioz Stars, 2019-07
    95/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: Typically, biomarker analytes are vastly outnumbered by endogenous proteins and macromolecules found in unprocessed patient samples, ensuring that the diagnostic assay can specifically and sensitively detect the target within a complex solution, which is imperative. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Blocking Assay:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: Paragraph title: Slide Fabrication and Blocking ... Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Microarray:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: 2.1 Corning epoxy coated glass slides were used as the DNA microarray substrate. .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Article Title: Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development
    Article Snippet: Paragraph title: 2.5. Microarray experiments ... In the study of the immobilization conditions, an array of rabbit immunoglobulin G, carbonic anhydrase, bovine serum albumin, ovalbumin, β-lactoglobulin B, α-lactalbumin and lysozyme at 1 mg/mL concentration was patterned using an SciFlexArrayer S5 spotter from Scienion.

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: For hybridoma screening studies, cholesterol (chol) was made up at a molar weight five times that of all other glycolipids. .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA). .. Slides were then blocked with 2% BSA/PBS at room temperature for 1 h. Serum samples (100 μl, 1:50 dilution in 1% BSA/PBS), purified anti-sulfatide antibody (10 μg/ml of IgM or 1 μg/ml of IgG) or neat hybridoma supernatants were applied to the slides for 1 h at 4 °C, then washed twice for 15 min in 1% BSA/PBS.

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Paragraph title: Protein Preparation and Microarray Printing ... Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer.

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: Paragraph title: Microarray preparation ... The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany).

    Article Title: Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
    Article Snippet: Paragraph title: EW microarray construction ... Microarrays were printed with a SciFLEXARRAYER S3 (Scienion) piezoelectric printer equipped with a 90 μm glass nozzle with a hydrophobic coating.

    Incubation:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment. .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance
    Article Snippet: The samples were printed with a non-contact piezo spotter, sciFlexxarrayer S5 (Scienion, Berlin, Germany), in four replicate spots per sample and subarray, and two subarrays per slide onto nitrocellulose coated ONCYTE-slides (Grace Bio Labs, Bend, USA). .. The samples were printed with a non-contact piezo spotter, sciFlexxarrayer S5 (Scienion, Berlin, Germany), in four replicate spots per sample and subarray, and two subarrays per slide onto nitrocellulose coated ONCYTE-slides (Grace Bio Labs, Bend, USA).

    Article Title: Digital Detection of Exosomes by Interferometric Imaging
    Article Snippet: Capture antibodies against human CD63, CD81, CD9, CD171 together with negative controls IgG (Goat or Rabbit, pure whole molecules) were arrayed on copoly-(DMA-NAS-MAPS)-coated SP-IRIS patterned silicon chips, with 100 nm oxide layer thickness, using a SciFlexArrayer S5 spotter from Scienion (Berlin, Germany). .. Capture antibodies against human CD63, CD81, CD9, CD171 together with negative controls IgG (Goat or Rabbit, pure whole molecules) were arrayed on copoly-(DMA-NAS-MAPS)-coated SP-IRIS patterned silicon chips, with 100 nm oxide layer thickness, using a SciFlexArrayer S5 spotter from Scienion (Berlin, Germany).

    Article Title: Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus
    Article Snippet: 3-Aminopropyltriethoxysilane–Glu modified GMR sensors were robotically printed with 500 μg/mL influenza A capture antibody (MAB8800; EMD Millipore Corporation, Temecula, CA, USA, specific to IAV NP) in a volume of 1.2 nL per sensor using the sci-FLEXARRAYER S5 (Scienion, Germany; Supplementary Figure ). .. 3-Aminopropyltriethoxysilane–Glu modified GMR sensors were robotically printed with 500 μg/mL influenza A capture antibody (MAB8800; EMD Millipore Corporation, Temecula, CA, USA, specific to IAV NP) in a volume of 1.2 nL per sensor using the sci-FLEXARRAYER S5 (Scienion, Germany; Supplementary Figure ).

    Article Title: Evolving serodiagnostics by rationally designed peptide arrays: the Burkholderia paradigm in Cystic Fibrosis
    Article Snippet: Microarrays were prepared using a non-contact sciFLEXARRAYER S3 (Scienion Co., Berlin, Germany) spotter. .. All the peptide samples were printed in triplicates, each sub-array has positional controls (Cy3-streptavidin) and positive controls (human IgG at the concentration of 0.2 mg/mL).

    Article Title: Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors
    Article Snippet: The chip was then cured at 120 °C on a hot plate for 1 h, followed by treatment with 2% poly(ethylene-alt-maleic anhydride) for 5 min. After the chip was rinsed with distilled water, a 1:1 mixture of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) in distilled water was added to the well, and incubated with the chip for 1 h. The chip was then rinsed again, and fully dried. .. Each capture antibody (anti-IL-6: MAB406, anti-GCSF: MAB414, anti-Eotaxin: AF-420-NA, anti-TNF-α: AF-410-NA, anti-FLT3LG: AF427, and anti-VEGF: AF-493-NA from R & D Systems) was spotted on different magneto-nanosensors in quadruplicate using a non-contact arrayer (sciFLEXARRAYER S5, Scienion).

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. IRIS images were taken for each chip to determine the initial dsDNA densities corresponding to each spot in the microarray.

    Article Title: Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses
    Article Snippet: The antibody microarrays are robotically printed using the S3 Flexarrayer (Scienion AG). .. Each antibody probe is spotted at high concentration ( > 2.0 milligrams/mL) in PBS.

    Modification:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: Sense and antisense oligonucleotides (Table ) were designed to capture antisense and sense strands of probes generated by the dendrimer and the modified PCR based labelling methods respectively. .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Article Title: Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus
    Article Snippet: The terminal aldehyde groups generated on the sensor surface allow subsequent covalent bonding of biomolecules containing amino groups onto GMR sensor ( , , ). .. 3-Aminopropyltriethoxysilane–Glu modified GMR sensors were robotically printed with 500 μg/mL influenza A capture antibody (MAB8800; EMD Millipore Corporation, Temecula, CA, USA, specific to IAV NP) in a volume of 1.2 nL per sensor using the sci-FLEXARRAYER S5 (Scienion, Germany; Supplementary Figure ). .. For the control reactions bovine serum albumin (BSA; 1 mg/mL) and biotinylated bovine serum albumin (biotin-BSA; 1 mg/mL) were similarly printed onto GMR sensors.

    Western Blot:

    Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance
    Article Snippet: Cell lysates were prepared as for Western Blotting. .. The samples were printed with a non-contact piezo spotter, sciFlexxarrayer S5 (Scienion, Berlin, Germany), in four replicate spots per sample and subarray, and two subarrays per slide onto nitrocellulose coated ONCYTE-slides (Grace Bio Labs, Bend, USA).

    Protein Array:

    Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance
    Article Snippet: The samples were printed with a non-contact piezo spotter, sciFlexxarrayer S5 (Scienion, Berlin, Germany), in four replicate spots per sample and subarray, and two subarrays per slide onto nitrocellulose coated ONCYTE-slides (Grace Bio Labs, Bend, USA). .. The samples were printed with a non-contact piezo spotter, sciFlexxarrayer S5 (Scienion, Berlin, Germany), in four replicate spots per sample and subarray, and two subarrays per slide onto nitrocellulose coated ONCYTE-slides (Grace Bio Labs, Bend, USA).

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer. .. Microarray slides coated with ultrathin nitrocellulose (PATH, Gentel Biosciences) were pre-gridded into 52 identical sub-arrays by using a wax imprinter (Gel Company).

    High Performance Liquid Chromatography:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: HPLC purified oligonucleotides were purchased from MWG Eurofins (Germany). .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Flow Cytometry:

    Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity
    Article Snippet: We flow reagents through the microfluidic chip over the sensor similar to the binding and adhesion experiments. .. Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Flexarrayer S5.

    Biomarker Assay:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: Typically, biomarker analytes are vastly outnumbered by endogenous proteins and macromolecules found in unprocessed patient samples, ensuring that the diagnostic assay can specifically and sensitively detect the target within a complex solution, which is imperative. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Hemagglutination Assay:

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer. .. 30 autoantigens and 4 control proteins were spotted onto each subarray in triplicate, resulting in a 10×10 protein array within each subarray.

    Generated:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: Sense and antisense oligonucleotides (Table ) were designed to capture antisense and sense strands of probes generated by the dendrimer and the modified PCR based labelling methods respectively. .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    other:

    Article Title: Picoliter‐volume inkjet printing into planar microdevice reservoirs for low‐waste, high‐capacity drug loading
    Article Snippet: 2.4 Drug printing was performed using the sciFLEXARRAYER S3 (Scienion AG, Johannisthal, Germany) (Figure ).

    Imaging:

    Article Title: Ultrasensitive interferometric on-chip microscopy of transparent objects
    Article Snippet: The corresponding OPDs recovered from LIM imaging using the PSI method agreed with the AFM measurements. .. An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a sciFLEXARRAYER S3 spotter (Scienion; see ).

    Protein Concentration:

    Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance
    Article Snippet: Cell lysates were mixed 1:2 with 2× Protein Arraying Buffer (Whatman, Brentfort, UK) to yield a final protein concentration of 1.5 μg/μL. .. The samples were printed with a non-contact piezo spotter, sciFlexxarrayer S5 (Scienion, Berlin, Germany), in four replicate spots per sample and subarray, and two subarrays per slide onto nitrocellulose coated ONCYTE-slides (Grace Bio Labs, Bend, USA).

    Polymerase Chain Reaction:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: Sense and antisense oligonucleotides (Table ) were designed to capture antisense and sense strands of probes generated by the dendrimer and the modified PCR based labelling methods respectively. .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Injection:

    Article Title: A fast intraoperative PTH point-of-care assay on the Philips handheld magnotech system
    Article Snippet: The optical part is the plastic bottom part of the integrated cartridge, comprising the detection surface and was formed by the injection molding of high binding microtiter plate substrate material. .. The detection spot was functionalized with anti-C-terminal PTH antibody (2 nL; 175 μg/mL), using an inkjet printer (sciFLEXARRAYER S3, Scienion AG), as described by Dittmer et.al.

    Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity
    Article Snippet: Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Flexarrayer S5. .. A syringe tube is loaded with 30 uL CRP (concentrations ranging from 10−3 ng/mL to 103 ng/mL), 30 uL biotinylated anti-CRP detection antibody (2 ug/mL) and 15 uL streptavidin-coated superparamagnetic nanoparticles (depending on the assay either Miltenyi Biotec MACS at 2 · 1012 particles/mL, or RnD MagCellect at 6 · 1010 particles/mL, details in ), each separated by an air bubble.

    Binding Assay:

    Article Title: A fast intraoperative PTH point-of-care assay on the Philips handheld magnotech system
    Article Snippet: The optical part is the plastic bottom part of the integrated cartridge, comprising the detection surface and was formed by the injection molding of high binding microtiter plate substrate material. .. The detection spot was functionalized with anti-C-terminal PTH antibody (2 nL; 175 μg/mL), using an inkjet printer (sciFLEXARRAYER S3, Scienion AG), as described by Dittmer et.al.

    Article Title: Real-Time Capture and Visualization of Individual Viruses in Complex Media
    Article Snippet: The sensor surface was coated in an antifouling copolymer with NHS groups to promote binding antibody probes. .. A Scienion S3 SciFlexArrayer was used to create an array of antibody probes.

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: The presence of other macro-molecules can lead to nonspecific binding which decreases the test confidence. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. IRIS images were taken for each chip to determine the initial dsDNA densities corresponding to each spot in the microarray.

    Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity
    Article Snippet: We flow reagents through the microfluidic chip over the sensor similar to the binding and adhesion experiments. .. Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Flexarrayer S5.

    Transmission Electron Microscopy:

    Article Title: Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles
    Article Snippet: The Scienion SciFLEXARRAYER S3 (See ) is a commercial, fully programmable, computer controlled liquid handling system that transfers specific picoliter sample volumes utilizing Piezo Dispense Capillaries (PDCs). .. The Scienion SciFLEXARRAYER S3 (See ) is a commercial, fully programmable, computer controlled liquid handling system that transfers specific picoliter sample volumes utilizing Piezo Dispense Capillaries (PDCs).

    Fluorescence:

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: For hybridoma screening studies, cholesterol (chol) was made up at a molar weight five times that of all other glycolipids. .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA). .. Slides were then blocked with 2% BSA/PBS at room temperature for 1 h. Serum samples (100 μl, 1:50 dilution in 1% BSA/PBS), purified anti-sulfatide antibody (10 μg/ml of IgM or 1 μg/ml of IgG) or neat hybridoma supernatants were applied to the slides for 1 h at 4 °C, then washed twice for 15 min in 1% BSA/PBS.

    Size-exclusion Chromatography:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. The chips were then incubated with 0, 200, 400, and 800 nM of purified gonococcal Fur protein , , , in 2 mL of binding buffer (20 mM Tris-Cl pH 7.9, 5 mM MgCl2 , 40 mM KCl, 0.125 mM MnCl2 , 2 mM DTT, 10% Glycerol, 0.19 µg/µL poly dIdC), respectively at RT for 2 hr.

    Purification:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: HPLC purified oligonucleotides were purchased from MWG Eurofins (Germany). .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: Serum samples, hybridoma supernatants and purified antibodies were screened against single glycolipids and glycolipid complexes printed using a glycolipid microarray ( ). .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA).

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Recombinant protein generation was outsourced to Origene (Rockville, MD), where protein production was performed in CHO cells and the proteins were purified to 80-90% purity. .. Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer.

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. IRIS images were taken for each chip to determine the initial dsDNA densities corresponding to each spot in the microarray.

    Construct:

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody. .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Chromatin Immunoprecipitation:

    Article Title: Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors
    Article Snippet: The chip was then cured at 120 °C on a hot plate for 1 h, followed by treatment with 2% poly(ethylene-alt-maleic anhydride) for 5 min. After the chip was rinsed with distilled water, a 1:1 mixture of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) in distilled water was added to the well, and incubated with the chip for 1 h. The chip was then rinsed again, and fully dried. .. Each capture antibody (anti-IL-6: MAB406, anti-GCSF: MAB414, anti-Eotaxin: AF-420-NA, anti-TNF-α: AF-410-NA, anti-FLT3LG: AF427, and anti-VEGF: AF-493-NA from R & D Systems) was spotted on different magneto-nanosensors in quadruplicate using a non-contact arrayer (sciFLEXARRAYER S5, Scienion).

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany). .. After a 12 hr immobilization period, spotted chips were washed with Saline Sodium Citrate ( SSC ) buffer in decreasing concentrations (2x, 0.2x, and 0.1x) to produce a microarray of different covalently-attached dsDNA probes.

    Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity
    Article Snippet: We flow reagents through the microfluidic chip over the sensor similar to the binding and adhesion experiments. .. Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Flexarrayer S5.

    Article Title: Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses
    Article Snippet: The SP-IRIS chip consists of a silicon substrate with a thin silicon dioxide top layer. .. The antibody microarrays are robotically printed using the S3 Flexarrayer (Scienion AG).

    Software:

    Article Title: Quantification of DNA and Protein Adsorption by Optical Phase Shift
    Article Snippet: Protein and DNA were serially diluted with milli-Q water using high precision pipettes from Gilson (Middleton, WI) down to the final concentrations reported in . .. The solutions were spotted using a Scienion SciFlexarrayer S5 (Berlin, Germany) piezoelectric arrayer equipped with the sciDropVOLUME software, a tool to measure the absolute volume of the droplets dispensed by a SciFlexarrayer piezo nozzle. .. For each spot, a photograph of the actual droplet in mid air is used to calculate the spotted volume.

    Hybridization:

    Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur
    Article Snippet: Amino-labeled forward DNA strands (50 µM) ( ) were mixed with excess complementary strands to create dsDNA through standard hybridization methods: mixed DNA solutions were heated to 80°C for 10 min and then allowed to cool to room temperature in a slow, controlled manner ( < 1°C/min). .. The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the sciFLEXARRAYER S3 (Scienion, Inc, Berlin, Germany).

    Negative Control:

    Article Title: Real-Time Capture and Visualization of Individual Viruses in Complex Media
    Article Snippet: For a negative control antibody, an antibody against native VSV was used (8G5). .. A Scienion S3 SciFlexArrayer was used to create an array of antibody probes.

    Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics
    Article Snippet: An antibody against the wtVSV surface glycoprotein (8G5 monoclonal antibody) and a negative control monoclonal antibody (specific for the Marburg virus glycoprotein) were arrayed onto the sensor described in Section B-I, which is functionalized with the MCP-2 co-polymer from Lucidant Polymers LLC (Sunnyvale, CA, USA). .. The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody.

    Recombinant:

    Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels
    Article Snippet: Recombinant protein generation was outsourced to Origene (Rockville, MD), where protein production was performed in CHO cells and the proteins were purified to 80-90% purity. .. Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion sciFLEXARRAYER S3 ultra low volume non- contact microarrayer.

    Sample Prep:

    Article Title: Quantification of DNA and Protein Adsorption by Optical Phase Shift
    Article Snippet: Paragraph title: Sample preparation and spotting of biomolecules ... The solutions were spotted using a Scienion SciFlexarrayer S5 (Berlin, Germany) piezoelectric arrayer equipped with the sciDropVOLUME software, a tool to measure the absolute volume of the droplets dispensed by a SciFlexarrayer piezo nozzle.

    Evaporation:

    Article Title: Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles
    Article Snippet: The Scienion SciFLEXARRAYER S3 (See ) is a commercial, fully programmable, computer controlled liquid handling system that transfers specific picoliter sample volumes utilizing Piezo Dispense Capillaries (PDCs). .. The Scienion SciFLEXARRAYER S3 (See ) is a commercial, fully programmable, computer controlled liquid handling system that transfers specific picoliter sample volumes utilizing Piezo Dispense Capillaries (PDCs).

    Produced:

    Article Title: Real-Time Capture and Visualization of Individual Viruses in Complex Media
    Article Snippet: A Scienion S3 SciFlexArrayer was used to create an array of antibody probes. .. A Scienion S3 SciFlexArrayer was used to create an array of antibody probes.

    Concentration Assay:

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods
    Article Snippet: Each capture oligonucleotide was diluted in 0.15 M NaH2 PO4 spotting buffer (pH 8.5) to a final concentration of 25µM and transferred to a 96 well polypropylene plate (Greiner, UK). .. Slides were printed using a Scienion SciFlexarrayer S3 piezoelectric arrayer (Germany) according to the manufacturer’s protocol in a dust free environment.

    Article Title: Quantification of DNA and Protein Adsorption by Optical Phase Shift
    Article Snippet: After dialysis, the protein solutions were lyophilized and the resulting protein powders were re-dissolved by adding milli-Q water up to a final concentration of 2 mg/mL. .. The solutions were spotted using a Scienion SciFlexarrayer S5 (Berlin, Germany) piezoelectric arrayer equipped with the sciDropVOLUME software, a tool to measure the absolute volume of the droplets dispensed by a SciFlexarrayer piezo nozzle.

    Article Title: Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development
    Article Snippet: Slides were washed with water and dried under vacuum at 80 °C for 15 min. Copoly(DMA–NAS–MAPS) has been chosen for the easiness and reproducibility of the coating procedure and for its ability to functionalize silicon without altering its optical properties. .. In the study of the immobilization conditions, an array of rabbit immunoglobulin G, carbonic anhydrase, bovine serum albumin, ovalbumin, β-lactoglobulin B, α-lactalbumin and lysozyme at 1 mg/mL concentration was patterned using an SciFlexArrayer S5 spotter from Scienion. .. Every protein was spotted in three different buffers: PBS (pH 7.2), borate (borate/NaOH 50 mM pH 9) and acetate (6-EACA/acetate 20 mM pH 4.4).

    Article Title: Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses
    Article Snippet: The antibody microarrays are robotically printed using the S3 Flexarrayer (Scienion AG). .. The antibody microarrays are robotically printed using the S3 Flexarrayer (Scienion AG).

    Molecular Weight:

    Article Title: Ultrasensitive interferometric on-chip microscopy of transparent objects
    Article Snippet: An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a sciFLEXARRAYER S3 spotter (Scienion; see ). .. An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a sciFLEXARRAYER S3 spotter (Scienion; see ).

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    SCIENION microarray printer
    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid <t>microarray.</t> Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Microarray Printer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Journal: Journal of Neuroimmunology

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    doi: 10.1016/j.jneuroim.2018.07.004

    Figure Lengend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Article Snippet: A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA).

    Techniques: Microarray, Generated, Binding Assay, Mouse Assay