microspotter (SCIENION)
95
|
Name:
sciFLEXARRAYER S3
Description:
Picoliter precsion liquid handler
Catalog Number:
F003
Price:
None
Category:
Arrayer
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Structured Review
SCIENION
microspotter
Picoliter precsion liquid handler
https://www.bioz.com/result/microspotter/product/SCIENION
Average 95 stars, based on 3 article reviews
Price from $9.99 to $1999.99
Picoliter precsion liquid handler
https://www.bioz.com/result/microspotter/product/SCIENION
Average 95 stars, based on 3 article reviews
Price from $9.99 to $1999.99
microspotter - by Bioz Stars,
2019-05
95/100 stars
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Diagnostic Assay:Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics Article Snippet: Typically, biomarker analytes are vastly outnumbered by endogenous proteins and macromolecules found in unprocessed patient samples, ensuring that the diagnostic assay can specifically and sensitively detect the target within a complex solution, which is imperative. .. The array printed by the Blocking Assay:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: Paragraph title: Slide Fabrication and Blocking ... Slides were printed using a Scienion Microarray:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: 2.1 Corning epoxy coated glass slides were used as the DNA microarray substrate. .. Slides were printed using a Scienion Article Title: Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development Article Snippet: Paragraph title: 2.5. Microarray experiments ... In the study of the immobilization conditions, an array of rabbit immunoglobulin G, carbonic anhydrase, bovine serum albumin, ovalbumin, β-lactoglobulin B, α-lactalbumin and lysozyme at 1 mg/mL concentration was patterned using an Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes Article Snippet: For hybridoma screening studies, cholesterol (chol) was made up at a molar weight five times that of all other glycolipids. .. A Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels Article Snippet: Paragraph title: Protein Preparation and Microarray Printing ... Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: Paragraph title: Microarray preparation ... The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Article Title: Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray Article Snippet: Paragraph title: EW microarray construction ... Microarrays were printed with a Incubation:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: Slides were printed using a Scienion Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance Article Snippet: The samples were printed with a non-contact piezo spotter, Article Title: Digital Detection of Exosomes by Interferometric Imaging Article Snippet: Capture antibodies against human CD63, CD81, CD9, CD171 together with negative controls IgG (Goat or Rabbit, pure whole molecules) were arrayed on copoly-(DMA-NAS-MAPS)-coated SP-IRIS patterned silicon chips, with 100 nm oxide layer thickness, using a Article Title: Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus Article Snippet: 3-Aminopropyltriethoxysilane–Glu modified GMR sensors were robotically printed with 500 μg/mL influenza A capture antibody (MAB8800; EMD Millipore Corporation, Temecula, CA, USA, specific to IAV NP) in a volume of 1.2 nL per sensor using the Article Title: Evolving serodiagnostics by rationally designed peptide arrays: the Burkholderia paradigm in Cystic Fibrosis Article Snippet: Microarrays were prepared using a Article Title: Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors Article Snippet: The chip was then cured at 120 °C on a hot plate for 1 h, followed by treatment with 2% poly(ethylene-alt-maleic anhydride) for 5 min. After the chip was rinsed with distilled water, a 1:1 mixture of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) in distilled water was added to the well, and incubated with the chip for 1 h. The chip was then rinsed again, and fully dried. .. Each capture antibody (anti-IL-6: MAB406, anti-GCSF: MAB414, anti-Eotaxin: AF-420-NA, anti-TNF-α: AF-410-NA, anti-FLT3LG: AF427, and anti-VEGF: AF-493-NA from R & D Systems) was spotted on different magneto-nanosensors in quadruplicate using a Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics Article Snippet: The array printed by the Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Article Title: Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses Article Snippet: The antibody microarrays are robotically printed using the Modification:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: Sense and antisense oligonucleotides (Table ) were designed to capture antisense and sense strands of probes generated by the dendrimer and the modified PCR based labelling methods respectively. .. Slides were printed using a Scienion Article Title: Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus Article Snippet: The terminal aldehyde groups generated on the sensor surface allow subsequent covalent bonding of biomolecules containing amino groups onto GMR sensor ( , , ). .. 3-Aminopropyltriethoxysilane–Glu modified GMR sensors were robotically printed with 500 μg/mL influenza A capture antibody (MAB8800; EMD Millipore Corporation, Temecula, CA, USA, specific to IAV NP) in a volume of 1.2 nL per sensor using the Western Blot:Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance Article Snippet: Cell lysates were prepared as for Western Blotting. .. The samples were printed with a non-contact piezo spotter, Protein Array:Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance Article Snippet: The samples were printed with a non-contact piezo spotter, Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels Article Snippet: Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion High Performance Liquid Chromatography:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: HPLC purified oligonucleotides were purchased from MWG Eurofins (Germany). .. Slides were printed using a Scienion Flow Cytometry:Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity Article Snippet: We flow reagents through the microfluidic chip over the sensor similar to the binding and adhesion experiments. .. Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Biomarker Assay:Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics Article Snippet: Typically, biomarker analytes are vastly outnumbered by endogenous proteins and macromolecules found in unprocessed patient samples, ensuring that the diagnostic assay can specifically and sensitively detect the target within a complex solution, which is imperative. .. The array printed by the Hemagglutination Assay:Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels Article Snippet: Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion Generated:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: Sense and antisense oligonucleotides (Table ) were designed to capture antisense and sense strands of probes generated by the dendrimer and the modified PCR based labelling methods respectively. .. Slides were printed using a Scienion other:Article Title: Picoliter‐volume inkjet printing into planar microdevice reservoirs for low‐waste, high‐capacity drug loading Article Snippet: 2.4 Drug printing was performed using the Imaging:Article Title: Ultrasensitive interferometric on-chip microscopy of transparent objects Article Snippet: The corresponding OPDs recovered from LIM imaging using the PSI method agreed with the AFM measurements. .. An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a Protein Concentration:Article Title: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance Article Snippet: Cell lysates were mixed 1:2 with 2× Protein Arraying Buffer (Whatman, Brentfort, UK) to yield a final protein concentration of 1.5 μg/μL. .. The samples were printed with a non-contact piezo spotter, Polymerase Chain Reaction:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: Sense and antisense oligonucleotides (Table ) were designed to capture antisense and sense strands of probes generated by the dendrimer and the modified PCR based labelling methods respectively. .. Slides were printed using a Scienion Injection:Article Title: A fast intraoperative PTH point-of-care assay on the Philips handheld magnotech system Article Snippet: The optical part is the plastic bottom part of the integrated cartridge, comprising the detection surface and was formed by the injection molding of high binding microtiter plate substrate material. .. The detection spot was functionalized with anti-C-terminal PTH antibody (2 nL; 175 μg/mL), using an Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity Article Snippet: Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Binding Assay:Article Title: A fast intraoperative PTH point-of-care assay on the Philips handheld magnotech system Article Snippet: The optical part is the plastic bottom part of the integrated cartridge, comprising the detection surface and was formed by the injection molding of high binding microtiter plate substrate material. .. The detection spot was functionalized with anti-C-terminal PTH antibody (2 nL; 175 μg/mL), using an Article Title: Real-Time Capture and Visualization of Individual Viruses in Complex Media Article Snippet: The sensor surface was coated in an antifouling copolymer with NHS groups to promote binding antibody probes. .. A Scienion Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics Article Snippet: The presence of other macro-molecules can lead to nonspecific binding which decreases the test confidence. .. The array printed by the Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity Article Snippet: We flow reagents through the microfluidic chip over the sensor similar to the binding and adhesion experiments. .. Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Transmission Electron Microscopy:Article Title: Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles Article Snippet: The Scienion Fluorescence:Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes Article Snippet: For hybridoma screening studies, cholesterol (chol) was made up at a molar weight five times that of all other glycolipids. .. A Size-exclusion Chromatography:Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Purification:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: HPLC purified oligonucleotides were purchased from MWG Eurofins (Germany). .. Slides were printed using a Scienion Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes Article Snippet: Serum samples, hybridoma supernatants and purified antibodies were screened against single glycolipids and glycolipid complexes printed using a glycolipid microarray ( ). .. A Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels Article Snippet: Recombinant protein generation was outsourced to Origene (Rockville, MD), where protein production was performed in CHO cells and the proteins were purified to 80-90% purity. .. Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Construct:Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics Article Snippet: The array printed by the sciFlexarrayer S3 spotter (Scienion AG, Berlin, Germany) consisted of five replicates of each antibody. .. The array printed by the Chromatin Immunoprecipitation:Article Title: Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors Article Snippet: The chip was then cured at 120 °C on a hot plate for 1 h, followed by treatment with 2% poly(ethylene-alt-maleic anhydride) for 5 min. After the chip was rinsed with distilled water, a 1:1 mixture of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) in distilled water was added to the well, and incubated with the chip for 1 h. The chip was then rinsed again, and fully dried. .. Each capture antibody (anti-IL-6: MAB406, anti-GCSF: MAB414, anti-Eotaxin: AF-420-NA, anti-TNF-α: AF-410-NA, anti-FLT3LG: AF427, and anti-VEGF: AF-493-NA from R & D Systems) was spotted on different magneto-nanosensors in quadruplicate using a Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Article Title: High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity Article Snippet: We flow reagents through the microfluidic chip over the sensor similar to the binding and adhesion experiments. .. Capture antibodies are immobilized along with Biotin-BSA and Biotin controls on different individual sensors using a Scienion Article Title: Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses Article Snippet: The SP-IRIS chip consists of a silicon substrate with a thin silicon dioxide top layer. .. The antibody microarrays are robotically printed using the Software:Article Title: Quantification of DNA and Protein Adsorption by Optical Phase Shift Article Snippet: Protein and DNA were serially diluted with milli-Q water using high precision pipettes from Gilson (Middleton, WI) down to the final concentrations reported in . .. The solutions were spotted using a Scienion Hybridization:Article Title: A High-Throughput Method to Examine Protein-Nucleotide Interactions Identifies Targets of the Bacterial Transcriptional Regulatory Protein Fur Article Snippet: Amino-labeled forward DNA strands (50 µM) ( ) were mixed with excess complementary strands to create dsDNA through standard hybridization methods: mixed DNA solutions were heated to 80°C for 10 min and then allowed to cool to room temperature in a slow, controlled manner ( < 1°C/min). .. The dsDNA solutions were spotted onto the pre-functionalized sensor chips using a non-contact piezo-driven spotter, the Negative Control:Article Title: Real-Time Capture and Visualization of Individual Viruses in Complex Media Article Snippet: For a negative control antibody, an antibody against native VSV was used (8G5). .. A Scienion Article Title: An Interferometric Reflectance Imaging Sensor for Point of Care Viral Diagnostics Article Snippet: An antibody against the wtVSV surface glycoprotein (8G5 monoclonal antibody) and a negative control monoclonal antibody (specific for the Marburg virus glycoprotein) were arrayed onto the sensor described in Section B-I, which is functionalized with the MCP-2 co-polymer from Lucidant Polymers LLC (Sunnyvale, CA, USA). .. The array printed by the Recombinant:Article Title: Autoantibody biomarkers identified by proteomics methods distinguish ovarian cancer from non ovarian cancer with various CA-125 levels Article Snippet: Recombinant protein generation was outsourced to Origene (Rockville, MD), where protein production was performed in CHO cells and the proteins were purified to 80-90% purity. .. Proteins were diluted in PBS pH 7.4 at 0.1mg/mL in a 384 well source plate (Genetix), and loaded onto a Scienion Sample Prep:Article Title: Quantification of DNA and Protein Adsorption by Optical Phase Shift Article Snippet: Paragraph title: Sample preparation and spotting of biomolecules ... The solutions were spotted using a Scienion Evaporation:Article Title: Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles Article Snippet: The Scienion Produced:Article Title: Real-Time Capture and Visualization of Individual Viruses in Complex Media Article Snippet: A Scienion Concentration Assay:Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods Article Snippet: Each capture oligonucleotide was diluted in 0.15 M NaH2 PO4 spotting buffer (pH 8.5) to a final concentration of 25µM and transferred to a 96 well polypropylene plate (Greiner, UK). .. Slides were printed using a Scienion Article Title: Quantification of DNA and Protein Adsorption by Optical Phase Shift Article Snippet: After dialysis, the protein solutions were lyophilized and the resulting protein powders were re-dissolved by adding milli-Q water up to a final concentration of 2 mg/mL. .. The solutions were spotted using a Scienion Article Title: Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development Article Snippet: Slides were washed with water and dried under vacuum at 80 °C for 15 min. Copoly(DMA–NAS–MAPS) has been chosen for the easiness and reproducibility of the coating procedure and for its ability to functionalize silicon without altering its optical properties. .. In the study of the immobilization conditions, an array of rabbit immunoglobulin G, carbonic anhydrase, bovine serum albumin, ovalbumin, β-lactoglobulin B, α-lactalbumin and lysozyme at 1 mg/mL concentration was patterned using an Article Title: Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses Article Snippet: The antibody microarrays are robotically printed using the Molecular Weight:Article Title: Ultrasensitive interferometric on-chip microscopy of transparent objects Article Snippet: An array of monolayer spots of bovine serum albumin (BSA) was deposited on epoxysilane-coated glass ( ) using a |
