microarray printer  (SCIENION)

 
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    Name:
    sciFLEXARRAYER S3
    Description:
    Picoliter precsion liquid handler
    Catalog Number:
    f003
    Price:
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    Category:
    Arrayer
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    Structured Review

    SCIENION microarray printer
    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid <t>microarray.</t> Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Picoliter precsion liquid handler
    https://www.bioz.com/result/microarray printer/product/SCIENION
    Average 96 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    microarray printer - by Bioz Stars, 2020-08
    96/100 stars

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    Images

    1) Product Images from "Differential binding patterns of anti-sulfatide antibodies to glial membranes"

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    Journal: Journal of Neuroimmunology

    doi: 10.1016/j.jneuroim.2018.07.004

    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Figure Legend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Techniques Used: Microarray, Generated, Binding Assay, Mouse Assay

    2) Product Images from "Differential binding patterns of anti-sulfatide antibodies to glial membranes"

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    Journal: Journal of Neuroimmunology

    doi: 10.1016/j.jneuroim.2018.07.004

    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Figure Legend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Techniques Used: Microarray, Generated, Binding Assay, Mouse Assay

    Related Articles

    Microarray:

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA). .. Slides were then blocked with 2% BSA/PBS at room temperature for 1 h. Serum samples (100 μl, 1:50 dilution in 1% BSA/PBS), purified anti-sulfatide antibody (10 μg/ml of IgM or 1 μg/ml of IgG) or neat hybridoma supernatants were applied to the slides for 1 h at 4 °C, then washed twice for 15 min in 1% BSA/PBS.

    Article Title: Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Article Snippet: .. Before the printing of the protein microarray with a sciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) the print media need to be prepared. ..

    Article Title: Differential Glycosylation Expression in Injured Rat Spinal Cord Treated with Immunosuppressive Drug Cyclosporin-A
    Article Snippet: .. The lectins were printed at approximately 1 nL per feature on Nexterion Slide H microarray slides using a sciFLEXARRAYER S3 piezoelectric printer (Scienion AG, Berlin, Germany) as previously described. .. Each microarray slide contained eight replicate subarrays, with each lectin spotted in replicates of six per subarray.

    Selection:

    Article Title: Aleuria Aurantia Lectin (AAL)-Reactive Immunoglobulin G Rapidly Appears in Sera of Animals following Antigen Exposure
    Article Snippet: .. For the selection of an optimal capture antibody for AAL-reactive IgG detection, different anti-mouse, or rabbit antibodies or F(ab′)2 fragments were printed onto each of the 52 identical subbarrays on each PATH slide by using a Scienion FLEX ARRAYER S3 ultra low volume piezo microarrayer. ..

    Fluorescence:

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA). .. Slides were then blocked with 2% BSA/PBS at room temperature for 1 h. Serum samples (100 μl, 1:50 dilution in 1% BSA/PBS), purified anti-sulfatide antibody (10 μg/ml of IgM or 1 μg/ml of IgG) or neat hybridoma supernatants were applied to the slides for 1 h at 4 °C, then washed twice for 15 min in 1% BSA/PBS.

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    • microarray printer  (SCIENION)
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    SCIENION microarray printer
    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid <t>microarray.</t> Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Microarray Printer, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray printer/product/SCIENION
    Average 96 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    microarray printer - by Bioz Stars, 2020-08
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Journal: Journal of Neuroimmunology

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    doi: 10.1016/j.jneuroim.2018.07.004

    Figure Lengend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Article Snippet: A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA).

    Techniques: Microarray, Generated, Binding Assay, Mouse Assay

    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Journal: Journal of Neuroimmunology

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    doi: 10.1016/j.jneuroim.2018.07.004

    Figure Lengend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Article Snippet: A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA).

    Techniques: Microarray, Generated, Binding Assay, Mouse Assay