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SCIENION liquid dispenser
Liquid Dispenser, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SCIENION s3 non contact spotter
S3 Non Contact Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SCIENION sciflexarrayer s3
Sciflexarrayer S3, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sciflexarrayer s3/product/SCIENION
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Structured Review

SCIENION sciflexarrayer robot arrayer
The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with <t>sciFlexarrayer</t> robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
Sciflexarrayer Robot Arrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sciflexarrayer robot arrayer/product/SCIENION
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sciflexarrayer robot arrayer - by Bioz Stars, 2023-02
95/100 stars

Images

1) Product Images from "Toxicity Assays in Nanodrops Combining Bioassay and Morphometric Endpoints"

Article Title: Toxicity Assays in Nanodrops Combining Bioassay and Morphometric Endpoints

Journal: PLoS ONE

doi: 10.1371/journal.pone.0000163

The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with sciFlexarrayer robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
Figure Legend Snippet: The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with sciFlexarrayer robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.

Techniques Used: Cell Based Assay, Clone Assay, Staining


Structured Review

SCIENION inc s3 flexarrayer
Inc S3 Flexarrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inc s3 flexarrayer/product/SCIENION
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inc s3 flexarrayer - by Bioz Stars, 2023-02
95/100 stars

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SCIENION sciflexarrayer s3
Sciflexarrayer S3, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sciflexarrayer s3/product/SCIENION
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SCIENION flex arrayer s3
Flex Arrayer S3, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flex arrayer s3/product/SCIENION
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flex arrayer s3 - by Bioz Stars, 2023-02
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Structured Review

SCIENION s3 sciflexarrayer
S3 Sciflexarrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s3 sciflexarrayer/product/SCIENION
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sciflexarrayer s3 piezoelectric robotic arrayer  (SCIENION)

 
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    Structured Review

    SCIENION sciflexarrayer s3 piezoelectric robotic arrayer
    Sciflexarrayer S3 Piezoelectric Robotic Arrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sciflexarrayer s3 piezoelectric robotic arrayer/product/SCIENION
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sciflexarrayer s3 piezoelectric robotic arrayer - by Bioz Stars, 2023-02
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    Structured Review

    SCIENION sciflexarrayer s3 non contact microarray
    (A) Summary of mAb binding to GPCs by BLI (raw data in Fig. S6). The binding efficiency is based on the relative on-rate of IgG to immobilized GPC and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; -, minimal to no binding. Proposed IgG occupancy per GPC is estimated based on relative R max values under the assumption the highest R max indicates full occupancy and 37.7H has a preferred occupancy of 3 Fabs per trimer . (B) mAb neutralization of pseudoviruses derived from LASV strains of diverse lineages. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates (37.7H neutralization assay comparisons shown in Fig. S7A). (C) Thermostability of LASV LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the melting temperature ( T m ) of each complex. Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (D) Synthetic matriglycan binding competition <t>microarray</t> of StrepTagged GPC-A binding to matriglycan with and without pre-treatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB antibody (Fig. S7E). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP1 at a pH of 5 (Fig. S7F).
    Sciflexarrayer S3 Non Contact Microarray, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sciflexarrayer s3 non contact microarray/product/SCIENION
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    Images

    1) Product Images from "Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies"

    Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies

    Journal: bioRxiv

    doi: 10.1101/2022.09.26.509601

    (A) Summary of mAb binding to GPCs by BLI (raw data in Fig. S6). The binding efficiency is based on the relative on-rate of IgG to immobilized GPC and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; -, minimal to no binding. Proposed IgG occupancy per GPC is estimated based on relative R max values under the assumption the highest R max indicates full occupancy and 37.7H has a preferred occupancy of 3 Fabs per trimer . (B) mAb neutralization of pseudoviruses derived from LASV strains of diverse lineages. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates (37.7H neutralization assay comparisons shown in Fig. S7A). (C) Thermostability of LASV LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the melting temperature ( T m ) of each complex. Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (D) Synthetic matriglycan binding competition microarray of StrepTagged GPC-A binding to matriglycan with and without pre-treatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB antibody (Fig. S7E). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP1 at a pH of 5 (Fig. S7F).
    Figure Legend Snippet: (A) Summary of mAb binding to GPCs by BLI (raw data in Fig. S6). The binding efficiency is based on the relative on-rate of IgG to immobilized GPC and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; -, minimal to no binding. Proposed IgG occupancy per GPC is estimated based on relative R max values under the assumption the highest R max indicates full occupancy and 37.7H has a preferred occupancy of 3 Fabs per trimer . (B) mAb neutralization of pseudoviruses derived from LASV strains of diverse lineages. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates (37.7H neutralization assay comparisons shown in Fig. S7A). (C) Thermostability of LASV LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the melting temperature ( T m ) of each complex. Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (D) Synthetic matriglycan binding competition microarray of StrepTagged GPC-A binding to matriglycan with and without pre-treatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB antibody (Fig. S7E). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP1 at a pH of 5 (Fig. S7F).

    Techniques Used: Binding Assay, Neutralization, Derivative Assay, Nano Differential Scanning Fluorimetry, Microarray, Recombinant

    (A) BLI sensorgrams depicting immobilized GPC-I53-50A binding to S370.7 IgG in a dose-dependent manner. IgG concentrations used were 400, 200, 100, 50, 25, and 12.5 nM. K D value determined using a 1:1 binding profile and assuming partial dissociation. (B) LIV LASV pseudovirus nutralization of LASV by S370.7. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) BLI sensorgram comparing binding of S370.7 IgG to Fab to immobilized GPC. IgG and Fab were added at an equimolar concentration of 400 nM. (D) Thermostability of LIV GPC in complex with S370.7 assessed by nanoDSF. Points represent the melting temperature (T m ). Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (E) Synthetic matriglycan binding microarray of StrepTagged GPC-I53-50A bound to S370.7 IgG and detected using StrepMAB antibody. (F) BLI analysis of immobilized GPC bound to S370.7 or 25.10C IgG and then exposed to recombinant LAMP-1 at a pH of 5.
    Figure Legend Snippet: (A) BLI sensorgrams depicting immobilized GPC-I53-50A binding to S370.7 IgG in a dose-dependent manner. IgG concentrations used were 400, 200, 100, 50, 25, and 12.5 nM. K D value determined using a 1:1 binding profile and assuming partial dissociation. (B) LIV LASV pseudovirus nutralization of LASV by S370.7. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) BLI sensorgram comparing binding of S370.7 IgG to Fab to immobilized GPC. IgG and Fab were added at an equimolar concentration of 400 nM. (D) Thermostability of LIV GPC in complex with S370.7 assessed by nanoDSF. Points represent the melting temperature (T m ). Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (E) Synthetic matriglycan binding microarray of StrepTagged GPC-I53-50A bound to S370.7 IgG and detected using StrepMAB antibody. (F) BLI analysis of immobilized GPC bound to S370.7 or 25.10C IgG and then exposed to recombinant LAMP-1 at a pH of 5.

    Techniques Used: Binding Assay, Neutralization, Concentration Assay, Nano Differential Scanning Fluorimetry, Microarray, Recombinant

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    SCIENION liquid dispenser
    Liquid Dispenser, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SCIENION s3 non contact spotter
    S3 Non Contact Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SCIENION sciflexarrayer s3
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    SCIENION sciflexarrayer robot arrayer
    The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with <t>sciFlexarrayer</t> robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
    Sciflexarrayer Robot Arrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SCIENION inc s3 flexarrayer
    The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with <t>sciFlexarrayer</t> robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
    Inc S3 Flexarrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SCIENION flex arrayer s3
    The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with <t>sciFlexarrayer</t> robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
    Flex Arrayer S3, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flex arrayer s3/product/SCIENION
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    SCIENION s3 sciflexarrayer
    The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with <t>sciFlexarrayer</t> robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
    S3 Sciflexarrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SCIENION sciflexarrayer s3 piezoelectric robotic arrayer
    The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with <t>sciFlexarrayer</t> robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.
    Sciflexarrayer S3 Piezoelectric Robotic Arrayer, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sciflexarrayer s3 piezoelectric robotic arrayer/product/SCIENION
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    SCIENION sciflexarrayer s3 non contact microarray
    (A) Summary of mAb binding to GPCs by BLI (raw data in Fig. S6). The binding efficiency is based on the relative on-rate of IgG to immobilized GPC and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; -, minimal to no binding. Proposed IgG occupancy per GPC is estimated based on relative R max values under the assumption the highest R max indicates full occupancy and 37.7H has a preferred occupancy of 3 Fabs per trimer . (B) mAb neutralization of pseudoviruses derived from LASV strains of diverse lineages. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates (37.7H neutralization assay comparisons shown in Fig. S7A). (C) Thermostability of LASV LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the melting temperature ( T m ) of each complex. Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (D) Synthetic matriglycan binding competition <t>microarray</t> of StrepTagged GPC-A binding to matriglycan with and without pre-treatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB antibody (Fig. S7E). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP1 at a pH of 5 (Fig. S7F).
    Sciflexarrayer S3 Non Contact Microarray, supplied by SCIENION, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with sciFlexarrayer robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.

    Journal: PLoS ONE

    Article Title: Toxicity Assays in Nanodrops Combining Bioassay and Morphometric Endpoints

    doi: 10.1371/journal.pone.0000163

    Figure Lengend Snippet: The ‘Cell-on-Chip’ device was used to obtain four hundred independent HepG2 stress inducible fluorescent cell based assay measurements using two hsp promoter containing clones and four toxic at ten doses. The experiments were performed in quintuplicate measurements. 1a. Cell dispensing with sciFlexarrayer robot arrayer. 1b Zoom on the assembled mosaic of images corresponding to A10 clone after 6 h exposure to ten doses of Arsenate in quintuplicate (columns); the Hsp induction is monitored by the green EGFP signal, cell nucleus is stained in blue by Hoechst and cell cytoplasm is stained in red by Phalloïdin. 1c: Heterogeneity in cell response is illustrated by an example of Hsp response to 5 10 −5 M Arsenate exposure. Scale bar represents 500 µm. Fully automated image capture with a 10× objective and dedicated image analysis were performed using the same detection protocols by IMSTAR Pathfinder™ Cellscan system. All cells were individually segmented (contour highlighted in white) to extract information (signal intensity, morphology) on every single cell within each drop.

    Article Snippet: Cells were spotted using the sciFLEXARRAYER robot arrayer from Scienion AG (Berlin, Germany) as described previously .

    Techniques: Cell Based Assay, Clone Assay, Staining

    (A) Summary of mAb binding to GPCs by BLI (raw data in Fig. S6). The binding efficiency is based on the relative on-rate of IgG to immobilized GPC and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; -, minimal to no binding. Proposed IgG occupancy per GPC is estimated based on relative R max values under the assumption the highest R max indicates full occupancy and 37.7H has a preferred occupancy of 3 Fabs per trimer . (B) mAb neutralization of pseudoviruses derived from LASV strains of diverse lineages. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates (37.7H neutralization assay comparisons shown in Fig. S7A). (C) Thermostability of LASV LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the melting temperature ( T m ) of each complex. Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (D) Synthetic matriglycan binding competition microarray of StrepTagged GPC-A binding to matriglycan with and without pre-treatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB antibody (Fig. S7E). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP1 at a pH of 5 (Fig. S7F).

    Journal: bioRxiv

    Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies

    doi: 10.1101/2022.09.26.509601

    Figure Lengend Snippet: (A) Summary of mAb binding to GPCs by BLI (raw data in Fig. S6). The binding efficiency is based on the relative on-rate of IgG to immobilized GPC and is indicated as follows: +++, very strong binding; ++ strong binding; +, moderate binding; -, minimal to no binding. Proposed IgG occupancy per GPC is estimated based on relative R max values under the assumption the highest R max indicates full occupancy and 37.7H has a preferred occupancy of 3 Fabs per trimer . (B) mAb neutralization of pseudoviruses derived from LASV strains of diverse lineages. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates (37.7H neutralization assay comparisons shown in Fig. S7A). (C) Thermostability of LASV LIV GPC-I53-50A in complex with indicated Fabs assessed by nanoDSF. Points represent the melting temperature ( T m ) of each complex. Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (D) Synthetic matriglycan binding competition microarray of StrepTagged GPC-A binding to matriglycan with and without pre-treatment with 12.1F and 19.7E IgG. GPC-I53-50A bound to matriglycan was detected using StrepMAB antibody (Fig. S7E). (E) BLI competition analysis of immobilized GPC bound to indicated IgG and then exposed to recombinant LAMP1 at a pH of 5 (Fig. S7F).

    Article Snippet: All compounds were printed on NHS-ester activated glass slides (NEXTERION® Slide H, Schott Inc.) using a Scienion sciFLEXARRAYER S3 non-contact microarray equipped with a Scienion PDC80 nozzle (Scienion Inc.).

    Techniques: Binding Assay, Neutralization, Derivative Assay, Nano Differential Scanning Fluorimetry, Microarray, Recombinant

    (A) BLI sensorgrams depicting immobilized GPC-I53-50A binding to S370.7 IgG in a dose-dependent manner. IgG concentrations used were 400, 200, 100, 50, 25, and 12.5 nM. K D value determined using a 1:1 binding profile and assuming partial dissociation. (B) LIV LASV pseudovirus nutralization of LASV by S370.7. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) BLI sensorgram comparing binding of S370.7 IgG to Fab to immobilized GPC. IgG and Fab were added at an equimolar concentration of 400 nM. (D) Thermostability of LIV GPC in complex with S370.7 assessed by nanoDSF. Points represent the melting temperature (T m ). Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (E) Synthetic matriglycan binding microarray of StrepTagged GPC-I53-50A bound to S370.7 IgG and detected using StrepMAB antibody. (F) BLI analysis of immobilized GPC bound to S370.7 or 25.10C IgG and then exposed to recombinant LAMP-1 at a pH of 5.

    Journal: bioRxiv

    Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies

    doi: 10.1101/2022.09.26.509601

    Figure Lengend Snippet: (A) BLI sensorgrams depicting immobilized GPC-I53-50A binding to S370.7 IgG in a dose-dependent manner. IgG concentrations used were 400, 200, 100, 50, 25, and 12.5 nM. K D value determined using a 1:1 binding profile and assuming partial dissociation. (B) LIV LASV pseudovirus nutralization of LASV by S370.7. The dotted line indicates 50% neutralization. Data points represent the mean with error bars indicating the SEM of three technical replicates. (C) BLI sensorgram comparing binding of S370.7 IgG to Fab to immobilized GPC. IgG and Fab were added at an equimolar concentration of 400 nM. (D) Thermostability of LIV GPC in complex with S370.7 assessed by nanoDSF. Points represent the melting temperature (T m ). Each melting curve is a representative of triplicate curves with melting temperatures within ±0.1°C. (E) Synthetic matriglycan binding microarray of StrepTagged GPC-I53-50A bound to S370.7 IgG and detected using StrepMAB antibody. (F) BLI analysis of immobilized GPC bound to S370.7 or 25.10C IgG and then exposed to recombinant LAMP-1 at a pH of 5.

    Article Snippet: All compounds were printed on NHS-ester activated glass slides (NEXTERION® Slide H, Schott Inc.) using a Scienion sciFLEXARRAYER S3 non-contact microarray equipped with a Scienion PDC80 nozzle (Scienion Inc.).

    Techniques: Binding Assay, Neutralization, Concentration Assay, Nano Differential Scanning Fluorimetry, Microarray, Recombinant