microarray printer  (SCIENION)

 
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    Name:
    sciFLEXARRAYER S3
    Description:
    Picoliter precsion liquid handler
    Catalog Number:
    F003
    Price:
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    Category:
    Arrayer
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    SCIENION microarray printer
    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid <t>microarray.</t> Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Picoliter precsion liquid handler
    https://www.bioz.com/result/microarray printer/product/SCIENION
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microarray printer - by Bioz Stars, 2021-05
    96/100 stars

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    1) Product Images from "Differential binding patterns of anti-sulfatide antibodies to glial membranes"

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    Journal: Journal of Neuroimmunology

    doi: 10.1016/j.jneuroim.2018.07.004

    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Figure Legend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Techniques Used: Microarray, Generated, Binding Assay, Mouse Assay

    2) Product Images from "Differential binding patterns of anti-sulfatide antibodies to glial membranes"

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    Journal: Journal of Neuroimmunology

    doi: 10.1016/j.jneuroim.2018.07.004

    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Figure Legend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Techniques Used: Microarray, Generated, Binding Assay, Mouse Assay

    Related Articles

    other:

    Article Title: Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection
    Article Snippet: Printing of Test and Control Lines The membrane was printed with a streptavidin test line and an anti-mouse antibody control line using a Scienion sciFLEXARRAYER S3 with a type 4 piezo.

    Microarray:

    Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors
    Article Snippet: For mammalian lectin (or pathogen recognition receptor) microarrays, a panel of 16 recombinant and Ig-fusion mammalian lectins were reconstituted in phosphate buffered saline, pH 7.4 with 0.05% periodate-treated human serum albumin (HSA) ( ). .. Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described . .. For all microarrays, six replicate features per probe were printed, with approximately 1 nL per feature, using an uncoated 90 μm glass dispenser capillary and eight replicate subarrays were printed per microarray slide.

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA). .. Slides were then blocked with 2% BSA/PBS at room temperature for 1 h. Serum samples (100 μl, 1:50 dilution in 1% BSA/PBS), purified anti-sulfatide antibody (10 μg/ml of IgM or 1 μg/ml of IgG) or neat hybridoma supernatants were applied to the slides for 1 h at 4 °C, then washed twice for 15 min in 1% BSA/PBS.

    Fluorescence:

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes
    Article Snippet: .. A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA). .. Slides were then blocked with 2% BSA/PBS at room temperature for 1 h. Serum samples (100 μl, 1:50 dilution in 1% BSA/PBS), purified anti-sulfatide antibody (10 μg/ml of IgM or 1 μg/ml of IgG) or neat hybridoma supernatants were applied to the slides for 1 h at 4 °C, then washed twice for 15 min in 1% BSA/PBS.

    Amplification:

    Article Title: Clonal Decomposition and DNA Replication States Defined by Scaled Single-Cell Genome Sequencing
    Article Snippet: A Resource of Diverse, Annotated, Single-Cell Genomes Generated with Scalable Open Array Transposon-Mediated DNA Sequencing .. Scalable Single-Cell Library Preparation in Open Nanoliter Wells To scale amplification-free transposon-based single-cell genome sequencing to thousands of cells per library, we implemented a new platform called DLP+ by using commodity off the shelf components, principally comprised of a contactless piezoelectric dispenser (sciFLEXARRAYER S3 and cellenONE, Scienion) and open nanowell arrays (TakaraBio SmartChip; , A). .. Key elements include a large number of freely programmable reagent steps, real-time imaging, and object recognition allowing arrayed dispensing and doublet removal, bypassing limitations of Poisson loading.

    Sequencing:

    Article Title: Clonal Decomposition and DNA Replication States Defined by Scaled Single-Cell Genome Sequencing
    Article Snippet: A Resource of Diverse, Annotated, Single-Cell Genomes Generated with Scalable Open Array Transposon-Mediated DNA Sequencing .. Scalable Single-Cell Library Preparation in Open Nanoliter Wells To scale amplification-free transposon-based single-cell genome sequencing to thousands of cells per library, we implemented a new platform called DLP+ by using commodity off the shelf components, principally comprised of a contactless piezoelectric dispenser (sciFLEXARRAYER S3 and cellenONE, Scienion) and open nanowell arrays (TakaraBio SmartChip; , A). .. Key elements include a large number of freely programmable reagent steps, real-time imaging, and object recognition allowing arrayed dispensing and doublet removal, bypassing limitations of Poisson loading.

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    • microarray printer  (SCIENION)
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    SCIENION microarray printer
    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid <t>microarray.</t> Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p
    Microarray Printer, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray printer/product/SCIENION
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microarray printer - by Bioz Stars, 2021-05
    96/100 stars
    		  Buy from Supplier

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    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Journal: Journal of Neuroimmunology

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    doi: 10.1016/j.jneuroim.2018.07.004

    Figure Lengend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Article Snippet: A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA).

    Techniques: Microarray, Generated, Binding Assay, Mouse Assay

    Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Journal: Journal of Neuroimmunology

    Article Title: Differential binding patterns of anti-sulfatide antibodies to glial membranes

    doi: 10.1016/j.jneuroim.2018.07.004

    Figure Lengend Snippet: Monoclonal anti-sulfatide antibodies bind to sulfatide and associated complexes on lipid microarray. Newly generated monoclonal anti-sulfatide antibodies were screened against sulfatide antigen printed on a lipid microarray. The IgM antibodies were screened at 10 μg/ml and the IgG antibodies were screened at 1 μg/ml. Their binding patterns fell into two broad categories. (A) Group A comprises GAME-M5 and GAME-G3 antibodies that bind exclusively to sulfatide and sulfatide complexes that do not contain complex gangliosides at the concentrations used. (B) Group B comprises GAME-M2, GAME-M6 and GAME-M7 antibodies that bind strongly to sulfatide and are not affected by associated complexes. (C) O4 was screened for comparison purposes, and showed binding to sulfatide and associated complexes in a similar pattern as the Group B antibodies. (D) CST −/− (n = 6) and CST +/+ (n = 6) mice were passively immunised with 250 μg of GAME-G3 antibody. Anti-sulfatide antibody levels were significantly higher following immunisation on day 1 compared to day −1 (baseline) for both genotypes. Clearance from the circulation does not differ between the two genotypes, implying that clearance is not due to receptor-dependent uptake as has been seen for antibodies against gangliosides. Results represent two independent experiments each with 3 mice per group. * Significance at day 1 versus day −1, two-way ANOVA with Sidak's multiple comparison test, p

    Article Snippet: A microarray printer (sciFLEXARRAYER S3, Scienion, Berlin, Germany) was used to print glycolipid spots in a predefined pattern onto low fluorescence PVDF membrane-covered slides (Millipore, Billerica, MA, USA).

    Techniques: Microarray, Generated, Binding Assay, Mouse Assay