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( A ) Layout of the biomarker <t>microarray</t> (BMA) slide, showing the arrangement of capture antibodies and controls within each well. ( B ) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.
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Schematic of the workflow of the device, including: 1. microarray printing, 2. sample channel attachment, 3. sample addition, incubation and removal, 4. probe channel attachment, 5. probe addition, incubation and removal.

Journal: Lab on a Chip

Article Title: Sample-sparing multiplexed antibody Fc biomarker discovery using a reconfigurable integrated microfluidic platform

doi: 10.1039/d5lc00042d

Figure Lengend Snippet: Schematic of the workflow of the device, including: 1. microarray printing, 2. sample channel attachment, 3. sample addition, incubation and removal, 4. probe channel attachment, 5. probe addition, incubation and removal.

Article Snippet: Proteins were diluted in 150 mM PBS with various concentrations of glycerol, then printed on slides by a sciFLEXARRAYER S3 microarray printer (Scienion, German).

Techniques: Microarray, Incubation

Physical design of the device. (A) 2D layout of alignment marks and microarrays on the glass slide. (B) Optical image of an alignment mark used by PDMS attachment. (C) Optical image of an alignment mark used by microarray printing. (D) Optical image of a 5 × 5 array after printing. (E) PDMS middle layer for sample. (F) PDMS middle layer for probes. (G) Sample middle layer rectified on 3D printed alignment block. (H) Optical image of the device after full assembly. The sample version was used as the middle layer. (I) Demonstration of the assembled device with colored dye filling the channels as samples.

Journal: Lab on a Chip

Article Title: Sample-sparing multiplexed antibody Fc biomarker discovery using a reconfigurable integrated microfluidic platform

doi: 10.1039/d5lc00042d

Figure Lengend Snippet: Physical design of the device. (A) 2D layout of alignment marks and microarrays on the glass slide. (B) Optical image of an alignment mark used by PDMS attachment. (C) Optical image of an alignment mark used by microarray printing. (D) Optical image of a 5 × 5 array after printing. (E) PDMS middle layer for sample. (F) PDMS middle layer for probes. (G) Sample middle layer rectified on 3D printed alignment block. (H) Optical image of the device after full assembly. The sample version was used as the middle layer. (I) Demonstration of the assembled device with colored dye filling the channels as samples.

Article Snippet: Proteins were diluted in 150 mM PBS with various concentrations of glycerol, then printed on slides by a sciFLEXARRAYER S3 microarray printer (Scienion, German).

Techniques: Microarray, Blocking Assay

( A ) Layout of the biomarker microarray (BMA) slide, showing the arrangement of capture antibodies and controls within each well. ( B ) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.

Journal: Micromachines

Article Title: Portable Fluorescence Microarray Reader-Enabled Biomarker Panel Detection System for Point-of-Care Diagnosis of Lupus Nephritis

doi: 10.3390/mi16020156

Figure Lengend Snippet: ( A ) Layout of the biomarker microarray (BMA) slide, showing the arrangement of capture antibodies and controls within each well. ( B ) Illustration of the BMA slide design with 16 wells and its alignment within the slide holder for analysis. The numbers along the side correspond to the row numbers of each well, while the numbers along the bottom represent the column positions of the slide.

Article Snippet: The slides were prepared using a non-contact microarray printing robot (sciFLEXARRAYER S3; Scienion GmbH, Berlin, Germany), which printed capture antibodies for each biomarker in triplicate at a controlled drop volume of 450 ± 20 pL.

Techniques: Biomarker Assay, Microarray