f nucleatum atcc 51191  (ATCC)


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    ATCC f nucleatum atcc 51191
    F Nucleatum Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum subsp animalis atcc 51191  (ATCC)


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    ATCC f nucleatum subsp animalis atcc 51191
    A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. <t>nucleatum</t> subspecies and F. periodonticum . A DNA ladder is shown on the left.
    F Nucleatum Subsp Animalis Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The prevalence of Fusobacterium nucleatum subspecies in the oral cavity stratifies by local health status"

    Article Title: The prevalence of Fusobacterium nucleatum subspecies in the oral cavity stratifies by local health status

    Journal: bioRxiv

    doi: 10.1101/2023.10.25.563997

    A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. nucleatum subspecies and F. periodonticum . A DNA ladder is shown on the left.
    Figure Legend Snippet: A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. nucleatum subspecies and F. periodonticum . A DNA ladder is shown on the left.

    Techniques Used: Labeling

    f nucleatum subsp animalis atcc 51191  (ATCC)


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    ATCC f nucleatum subsp animalis atcc 51191
    <t>Fusobacterium</t> <t>nucleatum</t> tends to adhere to colorectal cancer cells and promote their proliferation. (A) A schematic diagram of bacterial adherence detection by SPRi. HCT 116 cells were taken as the example. Scale bar: 20 μm. (B) The bacterial adherence to CCD 841 CoN and HCT 116 cells at the single‐bacteria‐cell level. The adherence and deviation of F. nucleatum are shown, respectively. (C) The electrochemical response of Caco‐2 cells after in situ lysis. The cells were preincubated with F. nucleatum or mFadA protein for 24 h. Scan rate: 0.1 V/s. Temperature: 37 ± 0.5°C. (D) Wound‐healing assay and the quantification of wound area in CCD 841 CoN cells after treatment with F. nucleatum or mFadA. (E) Wound‐healing assay and the quantification of wound area in Caco‐2 cells after treatment with F. nucleatum or mFadA. (F) Cell cycle analysis of Caco‐2 cells detected by flow cytometry. The cells underwent a 24 h preincubation with F. nucleatum or mFadA. Data are expressed as mean with SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. The F. nucleatum , mFadA, and control groups are respectively marked in blue, yellow, and gray.
    F Nucleatum Subsp Animalis Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Disease‐associated gut microbiome and critical metabolomic alterations in patients with colorectal cancer"

    Article Title: Disease‐associated gut microbiome and critical metabolomic alterations in patients with colorectal cancer

    Journal: Cancer Medicine

    doi: 10.1002/cam4.6194

    Fusobacterium nucleatum tends to adhere to colorectal cancer cells and promote their proliferation. (A) A schematic diagram of bacterial adherence detection by SPRi. HCT 116 cells were taken as the example. Scale bar: 20 μm. (B) The bacterial adherence to CCD 841 CoN and HCT 116 cells at the single‐bacteria‐cell level. The adherence and deviation of F. nucleatum are shown, respectively. (C) The electrochemical response of Caco‐2 cells after in situ lysis. The cells were preincubated with F. nucleatum or mFadA protein for 24 h. Scan rate: 0.1 V/s. Temperature: 37 ± 0.5°C. (D) Wound‐healing assay and the quantification of wound area in CCD 841 CoN cells after treatment with F. nucleatum or mFadA. (E) Wound‐healing assay and the quantification of wound area in Caco‐2 cells after treatment with F. nucleatum or mFadA. (F) Cell cycle analysis of Caco‐2 cells detected by flow cytometry. The cells underwent a 24 h preincubation with F. nucleatum or mFadA. Data are expressed as mean with SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. The F. nucleatum , mFadA, and control groups are respectively marked in blue, yellow, and gray.
    Figure Legend Snippet: Fusobacterium nucleatum tends to adhere to colorectal cancer cells and promote their proliferation. (A) A schematic diagram of bacterial adherence detection by SPRi. HCT 116 cells were taken as the example. Scale bar: 20 μm. (B) The bacterial adherence to CCD 841 CoN and HCT 116 cells at the single‐bacteria‐cell level. The adherence and deviation of F. nucleatum are shown, respectively. (C) The electrochemical response of Caco‐2 cells after in situ lysis. The cells were preincubated with F. nucleatum or mFadA protein for 24 h. Scan rate: 0.1 V/s. Temperature: 37 ± 0.5°C. (D) Wound‐healing assay and the quantification of wound area in CCD 841 CoN cells after treatment with F. nucleatum or mFadA. (E) Wound‐healing assay and the quantification of wound area in Caco‐2 cells after treatment with F. nucleatum or mFadA. (F) Cell cycle analysis of Caco‐2 cells detected by flow cytometry. The cells underwent a 24 h preincubation with F. nucleatum or mFadA. Data are expressed as mean with SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. The F. nucleatum , mFadA, and control groups are respectively marked in blue, yellow, and gray.

    Techniques Used: In Situ, Lysis, Wound Healing Assay, Cell Cycle Assay, Flow Cytometry

    Fusobacterium nucleatum regulates the expression of the CHK2 gene in Caco‐2 cells. (A) Quantitative real‐time RT‐PCR assay of CHK2 gene expression in Caco‐2 cells. Based on the comparison with respective control samples, data are expressed as mean with SD. ** p < 0.01; *** p < 0.001; **** p < 0.0001. (B) Western blotting results showing the protein expression of Caco‐2 cells. (C) The detection of gene expression changes in Caco‐2 cells by flow cytometry. The cells were preincubated with F. nucleatum or mFadA for 24 h prior to the experiments.
    Figure Legend Snippet: Fusobacterium nucleatum regulates the expression of the CHK2 gene in Caco‐2 cells. (A) Quantitative real‐time RT‐PCR assay of CHK2 gene expression in Caco‐2 cells. Based on the comparison with respective control samples, data are expressed as mean with SD. ** p < 0.01; *** p < 0.001; **** p < 0.0001. (B) Western blotting results showing the protein expression of Caco‐2 cells. (C) The detection of gene expression changes in Caco‐2 cells by flow cytometry. The cells were preincubated with F. nucleatum or mFadA for 24 h prior to the experiments.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry

    f nucleatum subsp animalis atcc 51191  (ATCC)


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    ATCC f nucleatum subsp animalis atcc 51191
    F Nucleatum Subsp Animalis Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum atcc 51191  (ATCC)


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    ATCC f nucleatum atcc 51191
    F Nucleatum Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum ssp animalis atcc 51191  (ATCC)


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    ATCC f nucleatum ssp animalis atcc 51191
    F Nucleatum Ssp Animalis Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum ssp animalis atcc 51191 fna  (ATCC)


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    ATCC f nucleatum ssp animalis atcc 51191 fna
    Biofilm thickness of F. <t>nucleatum</t> subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.
    F Nucleatum Ssp Animalis Atcc 51191 Fna, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro"

    Article Title: Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro

    Journal: Frontiers in Oral Health

    doi: 10.3389/froh.2022.853618

    Biofilm thickness of F. nucleatum subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.
    Figure Legend Snippet: Biofilm thickness of F. nucleatum subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.

    Techniques Used:

    Micrographs of single-subspecies F. nucleatum biofilms grown on poly-L-lysine coated Thermanox coverslips. (A) Micrographs showing differences in biofilm architecture. White arrows indicate bacterial aggregates within the biofilm. 1000X magnification, scale bar 20 μm. (B) Micrographs showing cell-to-cell cohesion. White arrow heads indicate presumed water channels. 5000X magnification, scale bar 5 μm. Biofilms from two independent experiments grown in duplicates were imaged and representative micrographs are shown.
    Figure Legend Snippet: Micrographs of single-subspecies F. nucleatum biofilms grown on poly-L-lysine coated Thermanox coverslips. (A) Micrographs showing differences in biofilm architecture. White arrows indicate bacterial aggregates within the biofilm. 1000X magnification, scale bar 20 μm. (B) Micrographs showing cell-to-cell cohesion. White arrow heads indicate presumed water channels. 5000X magnification, scale bar 5 μm. Biofilms from two independent experiments grown in duplicates were imaged and representative micrographs are shown.

    Techniques Used:

    Bioinformatic analysis of adhesion proteins in F. nucleatum subspecies. (A) Conservation of adhesion proteins in ATCC strains of F. nucleatum . The heatmap shows the percentage of identity with the FNN25 adhesion proteins Aid1, CmpA, FadA, Fap2, FomA, RadD, YadA. Protein length and domain organisation are indicated on the right for each of FNN25 proteins. The domains are indicated and coloured differently: glycine zipper (orange); autotransporter (dark blue); adhesion protein (yellow); pectin lyase-like (pink); collagen binding domain (light blue); head domain of trimeric autotransporter adhesin (dark orange); coiled stalk of trimeric autotransporter adhesin (black); YadA-like membrane anchor domain (white). (B) CmpA and Fap2 phylogenetic tree. The CmpA branch is highlighted in yellow, the Fap2 branch is highlighted in pink. Black circles represent bootstrap values > 95. FNN25 proteins are coloured in orange; FNN23 proteins in green; FNA proteins in dark orange; FNV in pink; FNP in light blue. Protein structures and lengths are outlined on the right of the tree. Autotransporter domains are coloured in blue, pectin lyase-like domains in pink. Figures were drawn with seaborn (version 0.11.2) and R (version 4.1.2).
    Figure Legend Snippet: Bioinformatic analysis of adhesion proteins in F. nucleatum subspecies. (A) Conservation of adhesion proteins in ATCC strains of F. nucleatum . The heatmap shows the percentage of identity with the FNN25 adhesion proteins Aid1, CmpA, FadA, Fap2, FomA, RadD, YadA. Protein length and domain organisation are indicated on the right for each of FNN25 proteins. The domains are indicated and coloured differently: glycine zipper (orange); autotransporter (dark blue); adhesion protein (yellow); pectin lyase-like (pink); collagen binding domain (light blue); head domain of trimeric autotransporter adhesin (dark orange); coiled stalk of trimeric autotransporter adhesin (black); YadA-like membrane anchor domain (white). (B) CmpA and Fap2 phylogenetic tree. The CmpA branch is highlighted in yellow, the Fap2 branch is highlighted in pink. Black circles represent bootstrap values > 95. FNN25 proteins are coloured in orange; FNN23 proteins in green; FNA proteins in dark orange; FNV in pink; FNP in light blue. Protein structures and lengths are outlined on the right of the tree. Autotransporter domains are coloured in blue, pectin lyase-like domains in pink. Figures were drawn with seaborn (version 0.11.2) and R (version 4.1.2).

    Techniques Used: Binding Assay

    f nucleatum atcc 51191  (ATCC)


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    ATCC f nucleatum atcc 51191
    Binding of F. nucleatum <t>ATCC</t> <t>51191</t> to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    F Nucleatum Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis"

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.744184

    Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .

    Techniques Used: Binding Assay, Flow Cytometry, Recombinant, Incubation

    Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay

    Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Flow Cytometry, Derivative Assay

    Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Derivative Assay, Marker, Expressing

    Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Derivative Assay

    f nucleatum atcc 51191  (ATCC)


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    ATCC f nucleatum atcc 51191
    Binding of F. nucleatum <t>ATCC</t> <t>51191</t> to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    F Nucleatum Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum atcc 51191 - by Bioz Stars, 2024-02
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    1) Product Images from "Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis"

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.744184

    Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .

    Techniques Used: Binding Assay, Flow Cytometry, Recombinant, Incubation

    Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay

    Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Flow Cytometry, Derivative Assay

    Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Derivative Assay, Marker, Expressing

    Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Derivative Assay

    f nucleatum atcc 51191  (ATCC)


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    Structured Review

    ATCC f nucleatum atcc 51191
    Binding of F. nucleatum <t>ATCC</t> <t>51191</t> to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    F Nucleatum Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum atcc 51191/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f nucleatum atcc 51191 - by Bioz Stars, 2024-02
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    Images

    1) Product Images from "Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis"

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.744184

    Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .

    Techniques Used: Binding Assay, Flow Cytometry, Recombinant, Incubation

    Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay

    Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Flow Cytometry, Derivative Assay

    Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Derivative Assay, Marker, Expressing

    Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Derivative Assay

    f nucleatum atcc 51191 stimulation  (ATCC)


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    Structured Review

    ATCC f nucleatum atcc 51191 stimulation
    Binding of F. nucleatum <t>ATCC</t> <t>51191</t> to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    F Nucleatum Atcc 51191 Stimulation, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum atcc 51191 stimulation/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f nucleatum atcc 51191 stimulation - by Bioz Stars, 2024-02
    99/100 stars

    Images

    1) Product Images from "Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis"

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.744184

    Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .

    Techniques Used: Binding Assay, Flow Cytometry, Recombinant, Incubation

    Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay

    Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Flow Cytometry, Derivative Assay

    Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Derivative Assay, Marker, Expressing

    Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.
    Figure Legend Snippet: Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Techniques Used: Marker, Expressing, Derivative Assay

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    ATCC f nucleatum atcc 51191
    F Nucleatum Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum atcc 51191/product/ATCC
    Average 99 stars, based on 1 article reviews
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    86
    ATCC f nucleatum subsp animalis atcc 51191
    A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. <t>nucleatum</t> subspecies and F. periodonticum . A DNA ladder is shown on the left.
    F Nucleatum Subsp Animalis Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum subsp animalis atcc 51191/product/ATCC
    Average 86 stars, based on 1 article reviews
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    ATCC f nucleatum ssp animalis atcc 51191
    A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. <t>nucleatum</t> subspecies and F. periodonticum . A DNA ladder is shown on the left.
    F Nucleatum Ssp Animalis Atcc 51191, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum ssp animalis atcc 51191/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f nucleatum ssp animalis atcc 51191 - by Bioz Stars, 2024-02
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    99
    ATCC f nucleatum ssp animalis atcc 51191 fna
    Biofilm thickness of F. <t>nucleatum</t> subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.
    F Nucleatum Ssp Animalis Atcc 51191 Fna, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC f nucleatum atcc 51191 stimulation
    Binding of F. nucleatum <t>ATCC</t> <t>51191</t> to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .
    F Nucleatum Atcc 51191 Stimulation, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    Image Search Results


    A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. nucleatum subspecies and F. periodonticum . A DNA ladder is shown on the left.

    Journal: bioRxiv

    Article Title: The prevalence of Fusobacterium nucleatum subspecies in the oral cavity stratifies by local health status

    doi: 10.1101/2023.10.25.563997

    Figure Lengend Snippet: A) Location of subspecies-/species-specific primer sets in each Fusobacterium genome. Genes are labeled with their annotated function if available, while hypothetical proteins are unlabeled. Forward and reverse primer locations are illustrated with red arrows. Genes and primers are not drawn to scale. B) Representative example of subspecies/species primer specificity. All reactions were performed using a mixture of gDNA from the four F. nucleatum subspecies and F. periodonticum . A DNA ladder is shown on the left.

    Article Snippet: The znpA and 16S V3V4 rDNA gene sequences were obtained from the following strains: F. nucleatum subsp. nucleatum ATCC 25586, F. nucleatum subsp. animalis ATCC 51191, F. nucleatum subsp. polymorphum ATCC 10953, and F. nucleatum subsp. fusiforme strain ATCC 51190.

    Techniques: Labeling

    Biofilm thickness of F. nucleatum subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.

    Journal: Frontiers in Oral Health

    Article Title: Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro

    doi: 10.3389/froh.2022.853618

    Figure Lengend Snippet: Biofilm thickness of F. nucleatum subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.

    Article Snippet: The following type strains of F. nucleatum were obtained from the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) and were originally purchased from the American Tissue Culture Collection (ATCC): F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FNN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques:

    Micrographs of single-subspecies F. nucleatum biofilms grown on poly-L-lysine coated Thermanox coverslips. (A) Micrographs showing differences in biofilm architecture. White arrows indicate bacterial aggregates within the biofilm. 1000X magnification, scale bar 20 μm. (B) Micrographs showing cell-to-cell cohesion. White arrow heads indicate presumed water channels. 5000X magnification, scale bar 5 μm. Biofilms from two independent experiments grown in duplicates were imaged and representative micrographs are shown.

    Journal: Frontiers in Oral Health

    Article Title: Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro

    doi: 10.3389/froh.2022.853618

    Figure Lengend Snippet: Micrographs of single-subspecies F. nucleatum biofilms grown on poly-L-lysine coated Thermanox coverslips. (A) Micrographs showing differences in biofilm architecture. White arrows indicate bacterial aggregates within the biofilm. 1000X magnification, scale bar 20 μm. (B) Micrographs showing cell-to-cell cohesion. White arrow heads indicate presumed water channels. 5000X magnification, scale bar 5 μm. Biofilms from two independent experiments grown in duplicates were imaged and representative micrographs are shown.

    Article Snippet: The following type strains of F. nucleatum were obtained from the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) and were originally purchased from the American Tissue Culture Collection (ATCC): F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FNN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques:

    Bioinformatic analysis of adhesion proteins in F. nucleatum subspecies. (A) Conservation of adhesion proteins in ATCC strains of F. nucleatum . The heatmap shows the percentage of identity with the FNN25 adhesion proteins Aid1, CmpA, FadA, Fap2, FomA, RadD, YadA. Protein length and domain organisation are indicated on the right for each of FNN25 proteins. The domains are indicated and coloured differently: glycine zipper (orange); autotransporter (dark blue); adhesion protein (yellow); pectin lyase-like (pink); collagen binding domain (light blue); head domain of trimeric autotransporter adhesin (dark orange); coiled stalk of trimeric autotransporter adhesin (black); YadA-like membrane anchor domain (white). (B) CmpA and Fap2 phylogenetic tree. The CmpA branch is highlighted in yellow, the Fap2 branch is highlighted in pink. Black circles represent bootstrap values > 95. FNN25 proteins are coloured in orange; FNN23 proteins in green; FNA proteins in dark orange; FNV in pink; FNP in light blue. Protein structures and lengths are outlined on the right of the tree. Autotransporter domains are coloured in blue, pectin lyase-like domains in pink. Figures were drawn with seaborn (version 0.11.2) and R (version 4.1.2).

    Journal: Frontiers in Oral Health

    Article Title: Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro

    doi: 10.3389/froh.2022.853618

    Figure Lengend Snippet: Bioinformatic analysis of adhesion proteins in F. nucleatum subspecies. (A) Conservation of adhesion proteins in ATCC strains of F. nucleatum . The heatmap shows the percentage of identity with the FNN25 adhesion proteins Aid1, CmpA, FadA, Fap2, FomA, RadD, YadA. Protein length and domain organisation are indicated on the right for each of FNN25 proteins. The domains are indicated and coloured differently: glycine zipper (orange); autotransporter (dark blue); adhesion protein (yellow); pectin lyase-like (pink); collagen binding domain (light blue); head domain of trimeric autotransporter adhesin (dark orange); coiled stalk of trimeric autotransporter adhesin (black); YadA-like membrane anchor domain (white). (B) CmpA and Fap2 phylogenetic tree. The CmpA branch is highlighted in yellow, the Fap2 branch is highlighted in pink. Black circles represent bootstrap values > 95. FNN25 proteins are coloured in orange; FNN23 proteins in green; FNA proteins in dark orange; FNV in pink; FNP in light blue. Protein structures and lengths are outlined on the right of the tree. Autotransporter domains are coloured in blue, pectin lyase-like domains in pink. Figures were drawn with seaborn (version 0.11.2) and R (version 4.1.2).

    Article Snippet: The following type strains of F. nucleatum were obtained from the Periodontal Research Group culture collection (School of Dentistry, University of Birmingham, UK) and were originally purchased from the American Tissue Culture Collection (ATCC): F. nucleatum ssp. animalis ATCC 51191 (FNA), F. nucleatum ssp. fusiforme ATCC 51190 (FNF), F. nucleatum ssp. nucleatum ATCC 25586 (FNN25), F. nucleatum ssp. polymorphum ATCC 10593 (FNP), and F. nucleatum ssp. vincentii ATCC 49256 (FNV).

    Techniques: Binding Assay

    Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .

    Journal: Frontiers in Immunology

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    doi: 10.3389/fimmu.2021.744184

    Figure Lengend Snippet: Binding of F. nucleatum ATCC 51191 to Siglecs using flow cytometry. (A) Binding of F. nucleatum to recombinant Siglec-Fc proteins (in blue). (B) Binding of F. nucleatum to Siglec-7 in the presence of GD3 inhibitor (in green) or untreated cells (in blue). (C) Binding of F. nucleatum to WT or Siglec-7 -/- U-937 cells. Bacteria incubated with α-Fc-PE Ab only was used as a control (in red). Fn, F. nucleatum .

    Article Snippet: This acquired moMϕ phenotype was also observed using the macrophage like cell line U-937 after F. nucleatum ATCC 51191 stimulation, leading to high IL-10 and low TNFα levels ( ).

    Techniques: Binding Assay, Flow Cytometry, Recombinant, Incubation

    Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Journal: Frontiers in Immunology

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    doi: 10.3389/fimmu.2021.744184

    Figure Lengend Snippet: Binding of F. nucleatum ATCC 51191 cells, LPS or OMVs to Siglec-7-Fc. Immobilised F. nucleatum 51191 cells were tested for binding to (A) Siglec-7-Fc and the extracted LPS to (B) Siglec-7-Fc or Siglec-9-Fc by ELISA. (C) Immobilised OMVs extracted from F. nucleatum 51191 were tested for binding to Siglec-7-Fc by ELISA. PBS was used as control. Data shown are the mean of duplicates ± SD derived from one representative experiment reproduced in three independent experiments. Fn, F. nucleatum . (D) STD NMR analysis of the binding between Siglec-7 and partially depolymerised OPS from F. nucleatum ATCC 51191. The panel shows the superimposition of the reference 1 H NMR spectrum (in black) and STD NMR spectrum (in green), the 1 H- 13 C HSQC spectrum (blue/red) and the chemical structure of F. nucleatum OPS repeating units. Statistical analyses were performed by t-test (for panels 2Aand 2C) or one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Article Snippet: This acquired moMϕ phenotype was also observed using the macrophage like cell line U-937 after F. nucleatum ATCC 51191 stimulation, leading to high IL-10 and low TNFα levels ( ).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay

    Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.

    Journal: Frontiers in Immunology

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    doi: 10.3389/fimmu.2021.744184

    Figure Lengend Snippet: Effect of F. nucleatum ATCC 51191 on human myeloid cells. Analysis of (A) cytokine and (B) cell surface marker expression in moDCs or moMϕs by flow cytometry. Human cells were stimulated with F. nucleatum ATCC 51191 (in orange). Unstained cells (in red) and unstimulated (un/ted) cells (in blue) were used as controls. (C) Internalisation of F. nucleatum ATCC 51191 into moDCs or moMϕs. Images were taken with a 40X objective. For the cytokine quantification, data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ****p < 0.0001, n.s., not statistically difference.

    Article Snippet: This acquired moMϕ phenotype was also observed using the macrophage like cell line U-937 after F. nucleatum ATCC 51191 stimulation, leading to high IL-10 and low TNFα levels ( ).

    Techniques: Marker, Expressing, Flow Cytometry, Derivative Assay

    Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.

    Journal: Frontiers in Immunology

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    doi: 10.3389/fimmu.2021.744184

    Figure Lengend Snippet: Effect of F. nucleatum ATCC 51191-derived LPS or OMVs on human myeloid cells. Analysis of (A) F. nucleatum LPS or (B) F. nucleatum OMVs on cytokine production and (C) F. nucleatum LPS or (D) F. nucleatum OMVs on cell surface marker expression. Unstimulated (un/ted) cells (in blue) and unstained cells (in red) were used as controls. Data shown are the mean of triplicates ± SD derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. **p < 0.01, ****p < 0.0001, n.s., not statistically difference.

    Article Snippet: This acquired moMϕ phenotype was also observed using the macrophage like cell line U-937 after F. nucleatum ATCC 51191 stimulation, leading to high IL-10 and low TNFα levels ( ).

    Techniques: Derivative Assay, Marker, Expressing

    Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Journal: Frontiers in Immunology

    Article Title: Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis

    doi: 10.3389/fimmu.2021.744184

    Figure Lengend Snippet: Effect of Siglec-7 on F. nucleatum ATCC 51191 interaction with human immune cells. (A) Cytokine production of U-937-PMA (WT or Siglec-7 -/- ) stimulated with F. nucleatum ATCC 51191. Bars represent the median values from 3 technical replicates. (B) Cytokine production and (C) cell surface marker expression of Siglec-7 silenced moDCs (in orange) or scramble control cells (in blue) stimulated with F. nucleatum . Unstimulated (un/ted) cells (in green) and unstained cells (in red) were used as controls. For the cytokine and internalisation analyses, data shown are the mean of triplicates ± SD and duplicated ± SD, respectively, derived from one representative experiment reproduced in three independent experiments. Statistical analyses were performed by two-way ANOVA followed by Tukey’s test. P < 0.05 was considered as statistically significant. ***p < 0.001, ****p < 0.0001, n.s., not statistically difference.

    Article Snippet: This acquired moMϕ phenotype was also observed using the macrophage like cell line U-937 after F. nucleatum ATCC 51191 stimulation, leading to high IL-10 and low TNFα levels ( ).

    Techniques: Marker, Expressing, Derivative Assay