f nucleatum strains  (ATCC)


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    ATCC f nucleatum strains
    F Nucleatum Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum subsp polymorphum strains atcc 10953  (ATCC)


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    ATCC f nucleatum subsp polymorphum strains atcc 10953
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. <t>polymorphum</t> ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Subsp Polymorphum Strains Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing"

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    Journal: iScience

    doi: 10.1016/j.isci.2024.110157

    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    Figure Legend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Techniques Used: Binding Assay, Labeling, Incubation, Staining


    Figure Legend Snippet:

    Techniques Used: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software

    f nucleatum strains a fitc  (ATCC)


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    ATCC f nucleatum strains a fitc
    Siglec-7 binding to F. <t>nucleatum</t> strains (A) <t>FITC-labeled</t> F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Strains A Fitc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing"

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    Journal: iScience

    doi: 10.1016/j.isci.2024.110157

    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    Figure Legend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Techniques Used: Binding Assay, Labeling, Incubation, Staining

    F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).
    Figure Legend Snippet: F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Techniques Used: Plasmid Preparation, Expressing, Bacteria, Staining, Control, Incubation, Labeling

    RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).
    Figure Legend Snippet: RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Techniques Used: Immunoprecipitation, Mass Spectrometry, Negative Control, Labeling, Mutagenesis, Staining, Binding Assay, Two Tailed Test

    IgA does not block binding of Siglec-7-Ig to F. nucleatum (A) FITC-labeled Fnn 23726 WT or (B) Fnn 23726 ΔRadD were stained with increasing amounts of human serum IgA (0.5 μg, 1 μg, 2 μg, and 4 μg). Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. Quantification of the MFI of three independent stainings of (C) Fnn 23726 WT and (D) Fnn 23726 ΔRadD with human serum IgA is shown. (E) Staining of F. nucleatum 23726 WT with Siglec-7-Ig after preincubation of bacteria with increasing amounts of human serum IgA. Filled gray histograms represent staining with secondary antibody only. MFI values are indicated left of each histogram. (F) Quantification of three independent experiments showing the fold increase in MFI in the presence of 4 μg IgA relative to no blocking. Significance was tested using Student’s t test (two-tailed and unpaired).
    Figure Legend Snippet: IgA does not block binding of Siglec-7-Ig to F. nucleatum (A) FITC-labeled Fnn 23726 WT or (B) Fnn 23726 ΔRadD were stained with increasing amounts of human serum IgA (0.5 μg, 1 μg, 2 μg, and 4 μg). Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. Quantification of the MFI of three independent stainings of (C) Fnn 23726 WT and (D) Fnn 23726 ΔRadD with human serum IgA is shown. (E) Staining of F. nucleatum 23726 WT with Siglec-7-Ig after preincubation of bacteria with increasing amounts of human serum IgA. Filled gray histograms represent staining with secondary antibody only. MFI values are indicated left of each histogram. (F) Quantification of three independent experiments showing the fold increase in MFI in the presence of 4 μg IgA relative to no blocking. Significance was tested using Student’s t test (two-tailed and unpaired).

    Techniques Used: Blocking Assay, Binding Assay, Labeling, Staining, Bacteria, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software

    f nucleatum subsp nucleatum strain atcc 23726  (ATCC)


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    ATCC f nucleatum subsp nucleatum strain atcc 23726
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Subsp Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing"

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    Journal: iScience

    doi: 10.1016/j.isci.2024.110157

    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    Figure Legend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Techniques Used: Binding Assay, Labeling, Incubation, Staining

    F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).
    Figure Legend Snippet: F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Techniques Used: Plasmid Preparation, Expressing, Bacteria, Staining, Control, Incubation, Labeling

    RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).
    Figure Legend Snippet: RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Techniques Used: Immunoprecipitation, Mass Spectrometry, Negative Control, Labeling, Mutagenesis, Staining, Binding Assay, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software

    growth condition f nucleatum strain atcc10953  (ATCC)


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    ATCC growth condition f nucleatum strain atcc10953
    Growth Condition F Nucleatum Strain Atcc10953, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum strain vpi 4355  (ATCC)


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    ATCC f nucleatum strain vpi 4355
    F Nucleatum Strain Vpi 4355, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum strain vpi 4355  (ATCC)


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    ATCC f nucleatum strain vpi 4355
    F Nucleatum Strain Vpi 4355, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum strain atcc 23726  (ATCC)


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    ATCC f nucleatum strain atcc 23726
    F Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum strain 25586  (ATCC)


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    ATCC f nucleatum strain 25586
    F Nucleatum Strain 25586, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    f nucleatum strain atcc 23726  (ATCC)


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    ATCC f nucleatum strain atcc 23726
    Role of <t>Fusobacterium</t> <t>nucleatum</t> (F. nucleatum) in adverse pregnancy outcomes (APO). ( A ) Interaction between Fusobacterium adhesion A (FadA), with vascular endothelial cadherin (VE-Cadherin) to internalize F. nucleatum in endothelial cells for bacterial dissemination, leading to increased inflammatory cytokines causing chorioamnionitis and preterm birth. ( B ) Fusobacterium apoptosis-inducing protein 2 (Fap2) binds with D-galactose-β (1–3)-N-acetyl-D-galactosamine (Gal-GalNAc) on endothelial cells to localize into placenta by suppressing TIGIT mediated activation of T cells and natural killer (NK) cells. ( C ) Lipopolysaccharide (LPS) interacts with TLR 4 on endothelial cells to activate the NF-κB pathway, leading to an inflammatory cytokine storm causing placental inflammation.
    F Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Fusobacterium nucleatum : An Overview of Evidence, Demi-Decadal Trends, and Its Role in Adverse Pregnancy Outcomes and Various Gynecological Diseases, including Cancers"

    Article Title: Fusobacterium nucleatum : An Overview of Evidence, Demi-Decadal Trends, and Its Role in Adverse Pregnancy Outcomes and Various Gynecological Diseases, including Cancers

    Journal: Cells

    doi: 10.3390/cells13080717

    Role of Fusobacterium nucleatum (F. nucleatum) in adverse pregnancy outcomes (APO). ( A ) Interaction between Fusobacterium adhesion A (FadA), with vascular endothelial cadherin (VE-Cadherin) to internalize F. nucleatum in endothelial cells for bacterial dissemination, leading to increased inflammatory cytokines causing chorioamnionitis and preterm birth. ( B ) Fusobacterium apoptosis-inducing protein 2 (Fap2) binds with D-galactose-β (1–3)-N-acetyl-D-galactosamine (Gal-GalNAc) on endothelial cells to localize into placenta by suppressing TIGIT mediated activation of T cells and natural killer (NK) cells. ( C ) Lipopolysaccharide (LPS) interacts with TLR 4 on endothelial cells to activate the NF-κB pathway, leading to an inflammatory cytokine storm causing placental inflammation.
    Figure Legend Snippet: Role of Fusobacterium nucleatum (F. nucleatum) in adverse pregnancy outcomes (APO). ( A ) Interaction between Fusobacterium adhesion A (FadA), with vascular endothelial cadherin (VE-Cadherin) to internalize F. nucleatum in endothelial cells for bacterial dissemination, leading to increased inflammatory cytokines causing chorioamnionitis and preterm birth. ( B ) Fusobacterium apoptosis-inducing protein 2 (Fap2) binds with D-galactose-β (1–3)-N-acetyl-D-galactosamine (Gal-GalNAc) on endothelial cells to localize into placenta by suppressing TIGIT mediated activation of T cells and natural killer (NK) cells. ( C ) Lipopolysaccharide (LPS) interacts with TLR 4 on endothelial cells to activate the NF-κB pathway, leading to an inflammatory cytokine storm causing placental inflammation.

    Techniques Used: Activation Assay

    Role of Fusobacterium nucleatum (F. nucleatum) in gynecological diseases (GD). ( A ) F. nucleatum increases TAGLN expression in endometrial fibroblasts to convert them into endometriosis lesion-forming myofibroblast cells. ( B ) Schematic representation displaying the interdependent beneficial relationship between F. nucleatum and vaginal bacteria, ultimately leading to bacterial vaginosis.
    Figure Legend Snippet: Role of Fusobacterium nucleatum (F. nucleatum) in gynecological diseases (GD). ( A ) F. nucleatum increases TAGLN expression in endometrial fibroblasts to convert them into endometriosis lesion-forming myofibroblast cells. ( B ) Schematic representation displaying the interdependent beneficial relationship between F. nucleatum and vaginal bacteria, ultimately leading to bacterial vaginosis.

    Techniques Used: Expressing, Bacteria

    Role of Fusobacterium nucleatum ( F. nucleatum ) in gynecological cancers (GC). ( A ) Fusobacterium apoptosis-inducing protein 2 (Fap2) binds with D-galactose-β (1–3)-N-acetyl-D-galactosamine (Gal-GalNAc) on cancer cells to induce metastasis from primary tumor sites to other organs. ( B ) Lipopolysaccharide (LPS) on F. nucleatum activated TLR4/MyD88 pathway to induce chemoresistance in cancer cells. ( C ) Interaction between Fusobacterium adhesion A (FadA), with epithelial cadherin (E Cadherin) on cancer cells to increase b-catenin expression and induce cancer cell proliferation. ( D ) Reactive oxygen species (ROS) and extracellular vesicles secreted from F. nucleatum induce DNA damage in cancer cells to acquire further mutation and genomic instability.
    Figure Legend Snippet: Role of Fusobacterium nucleatum ( F. nucleatum ) in gynecological cancers (GC). ( A ) Fusobacterium apoptosis-inducing protein 2 (Fap2) binds with D-galactose-β (1–3)-N-acetyl-D-galactosamine (Gal-GalNAc) on cancer cells to induce metastasis from primary tumor sites to other organs. ( B ) Lipopolysaccharide (LPS) on F. nucleatum activated TLR4/MyD88 pathway to induce chemoresistance in cancer cells. ( C ) Interaction between Fusobacterium adhesion A (FadA), with epithelial cadherin (E Cadherin) on cancer cells to increase b-catenin expression and induce cancer cell proliferation. ( D ) Reactive oxygen species (ROS) and extracellular vesicles secreted from F. nucleatum induce DNA damage in cancer cells to acquire further mutation and genomic instability.

    Techniques Used: Expressing, Mutagenesis

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    ATCC f nucleatum strains
    F Nucleatum Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum strains/product/ATCC
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    86
    ATCC f nucleatum subsp polymorphum strains atcc 10953
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. <t>polymorphum</t> ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Subsp Polymorphum Strains Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC f nucleatum strains a fitc
    Siglec-7 binding to F. <t>nucleatum</t> strains (A) <t>FITC-labeled</t> F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Strains A Fitc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum strains a fitc/product/ATCC
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    86
    ATCC f nucleatum subsp nucleatum strain atcc 23726
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Subsp Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum subsp nucleatum strain atcc 23726/product/ATCC
    Average 86 stars, based on 1 article reviews
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    86
    ATCC growth condition f nucleatum strain atcc10953
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    Growth Condition F Nucleatum Strain Atcc10953, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/growth condition f nucleatum strain atcc10953/product/ATCC
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    86
    ATCC f nucleatum strain vpi 4355
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Strain Vpi 4355, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum strain vpi 4355/product/ATCC
    Average 86 stars, based on 1 article reviews
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    ATCC f nucleatum strain atcc 23726
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum strain atcc 23726/product/ATCC
    Average 86 stars, based on 1 article reviews
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    ATCC f nucleatum strain 25586
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Strain 25586, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum strain 25586/product/ATCC
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    Image Search Results


    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Article Snippet: While Fnn 23726 and Fnn 25586 both bound to Siglec-7-Ig, F. nucleatum subsp. polymorphum strains ATCC 10953 and 12230 (subsequently abbreviated as Fnp 10953 and Fnp 12230) showed little to no staining.

    Techniques: Binding Assay, Labeling, Incubation, Staining

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet:

    Article Snippet: While Fnn 23726 and Fnn 25586 both bound to Siglec-7-Ig, F. nucleatum subsp. polymorphum strains ATCC 10953 and 12230 (subsequently abbreviated as Fnp 10953 and Fnp 12230) showed little to no staining.

    Techniques: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software

    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Article Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies.

    Techniques: Binding Assay, Labeling, Incubation, Staining

    F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies.

    Techniques: Plasmid Preparation, Expressing, Bacteria, Staining, Control, Incubation, Labeling

    RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies.

    Techniques: Immunoprecipitation, Mass Spectrometry, Negative Control, Labeling, Mutagenesis, Staining, Binding Assay, Two Tailed Test

    IgA does not block binding of Siglec-7-Ig to F. nucleatum (A) FITC-labeled Fnn 23726 WT or (B) Fnn 23726 ΔRadD were stained with increasing amounts of human serum IgA (0.5 μg, 1 μg, 2 μg, and 4 μg). Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. Quantification of the MFI of three independent stainings of (C) Fnn 23726 WT and (D) Fnn 23726 ΔRadD with human serum IgA is shown. (E) Staining of F. nucleatum 23726 WT with Siglec-7-Ig after preincubation of bacteria with increasing amounts of human serum IgA. Filled gray histograms represent staining with secondary antibody only. MFI values are indicated left of each histogram. (F) Quantification of three independent experiments showing the fold increase in MFI in the presence of 4 μg IgA relative to no blocking. Significance was tested using Student’s t test (two-tailed and unpaired).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: IgA does not block binding of Siglec-7-Ig to F. nucleatum (A) FITC-labeled Fnn 23726 WT or (B) Fnn 23726 ΔRadD were stained with increasing amounts of human serum IgA (0.5 μg, 1 μg, 2 μg, and 4 μg). Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. Quantification of the MFI of three independent stainings of (C) Fnn 23726 WT and (D) Fnn 23726 ΔRadD with human serum IgA is shown. (E) Staining of F. nucleatum 23726 WT with Siglec-7-Ig after preincubation of bacteria with increasing amounts of human serum IgA. Filled gray histograms represent staining with secondary antibody only. MFI values are indicated left of each histogram. (F) Quantification of three independent experiments showing the fold increase in MFI in the presence of 4 μg IgA relative to no blocking. Significance was tested using Student’s t test (two-tailed and unpaired).

    Article Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies.

    Techniques: Blocking Assay, Binding Assay, Labeling, Staining, Bacteria, Two Tailed Test

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet:

    Article Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies.

    Techniques: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software

    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Binding Assay, Labeling, Incubation, Staining

    F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Plasmid Preparation, Expressing, Bacteria, Staining, Control, Incubation, Labeling

    RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Immunoprecipitation, Mass Spectrometry, Negative Control, Labeling, Mutagenesis, Staining, Binding Assay, Two Tailed Test

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet:

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software