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f nucleatum strain atcc 23726  (ATCC)


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    ATCC f nucleatum strain atcc 23726
    F Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f nucleatum strain atcc 23726 - by Bioz Stars, 2024-11
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    ATCC tractable strain f nucleatum ssp nucleatum atcc 23726
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    ATCC f nucleatum subsp nucleatum strain atcc 23726
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Subsp Nucleatum Strain Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum subsp nucleatum strain atcc 23726/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f nucleatum subsp nucleatum strain atcc 23726 - by Bioz Stars, 2024-11
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    86
    ATCC f nucleatum subsp nucleatum strains atcc 23726
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Subsp Nucleatum Strains Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum subsp nucleatum strains atcc 23726/product/ATCC
    Average 86 stars, based on 1 article reviews
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    f nucleatum subsp nucleatum strains atcc 23726 - by Bioz Stars, 2024-11
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    ATCC f nucleatum strain atcc 23726 genome
    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum <t>ATCC</t> <t>23726</t> ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.
    F Nucleatum Strain Atcc 23726 Genome, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f nucleatum strain atcc 23726 genome/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f nucleatum strain atcc 23726 genome - by Bioz Stars, 2024-11
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    Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Binding Assay, Labeling, Incubation, Staining

    F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: F. nucleatum subsp. nucleatum confers Siglec-7-dependent protection against NK cell-mediated cancer cell killing (A) Schematic representation of target cell killing by YTS EV or YTS Siglec-7 cells. YTS empty vector (EV) or YTS Siglec-7 cells are activated almost exclusively by CD48-expressing target cells via their activating receptor 2B4. In the presence of bacteria, killing of target cells is inhibited via Siglec-7. (B) Staining of YTS EV or YTS Siglec-7 cells with Siglec-7 (upper histograms) and 2B4 (lower histograms) antibodies. Filled gray histograms represent staining with isotype control and secondary antibody only, respectively. (C) Staining of 721.221 or (D) BCBL-1 cells for Siglec-7 ligands with Siglec-7-Ig or of CD48 expression using anti-CD48 antibodies. (E) YTS EV or YTS Siglec-7 cells were precincubated or not with F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) at a ratio of 1:20 for 30 min at 37°C. The cells were then incubated with Calcein AM-labeled 721.221 or (F) BCBL-1 cells at an E:T ratio of 10:1 for 4 h at 37°C and the Calcein release into the supernatant was quantified. Each graph shows data from three independent experiments and each independent experiment is colored differently (white, blue, and black). Each experiment was performed in triplicates. Significance was tested using mixed-effects analysis with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Plasmid Preparation, Expressing, Bacteria, Staining, Control, Incubation, Labeling

    RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet: RadD of F. nucleatum subsp. nucleatum is the bacterial ligand for Siglec-7 Lysates prepared from (A) F. nucleatum subsp. nucleatum ATCC 23726 WT ( Fnn 23726 WT) or (B) F. nucleatum subsp. nucleatum ATCC 23726 ΔRadD ( Fnn 23726 ΔRadD) were immunoprecipitated using Siglec-7-Ig, CEACAM1-Ig, or CEACAM1 ΔN-Ig. Immunoprecipitates were visualized by Coomassie blue and immunoprecipitated bands were analyzed by mass spectrometry. RadD levels were quantified relative to the negative control. RQ, relative quantification. (C) FITC-labeled Fnn 23726 WT or its ΔRadD mutant was stained with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of eight is shown. (D) Quantification of Siglec-7-Ig binding to Fnn 23726 WT and the ΔRadD mutant shown as fold change in median fluorescent intensity (MFI) relative to Fnn 23726 WT. Bars represent means of eight independent experiments. Statistical significance was assessed using a two-tailed unpaired t test (∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Immunoprecipitation, Mass Spectrometry, Negative Control, Labeling, Mutagenesis, Staining, Binding Assay, Two Tailed Test

    Journal: iScience

    Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

    doi: 10.1016/j.isci.2024.110157

    Figure Lengend Snippet:

    Article Snippet: F. nucleatum subp. nucleatum is known to bind to several immune receptors such as CEACAM1 and TIGIT to circumvent the immune response., , , Here, we assessed binding of FITC-labeled F. nucleatum subsp. nucleatum strain ATCC 23726 (subsequently abbreviated as Fnn 23726) to several different NK cell receptor fusion proteins in which the extracellular domain of the respective immune receptor is fused to the Fc portion of human IgG1 (containing the N297A mutation to abrogate Fc receptor binding).

    Techniques: Purification, Virus, Recombinant, Transfection, Protease Inhibitor, Staining, Software