f nucleatum atcc 25586  (ATCC)


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    Name:
    Fusobacterium nucleatum subsp nucleatum VPI 4355 1612A
    Description:

    Catalog Number:
    25586
    Price:
    None
    Applications:
    Media testingQuality control
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    Structured Review

    ATCC f nucleatum atcc 25586
    Effects of exogenous indole on planktonic and biofilm cells of F. <t>nucleatum</t> ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

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    Images

    1) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    2) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    3) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    4) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    5) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    6) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).
    Figure Legend Snippet: Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).

    Techniques Used: Mass Spectrometry

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    7) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).
    Figure Legend Snippet: Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).

    Techniques Used: Mass Spectrometry

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    8) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    9) Product Images from "Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria"

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.187.15.5330-5340.2005

    Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are
    Figure Legend Snippet: Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are

    Techniques Used: Sequencing

    10) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    11) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    12) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    13) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    14) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    15) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    16) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    17) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).
    Figure Legend Snippet: Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).

    Techniques Used: Mass Spectrometry

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    18) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).
    Figure Legend Snippet: Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).

    Techniques Used: Mass Spectrometry

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    19) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    20) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    21) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    22) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    23) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).
    Figure Legend Snippet: Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).

    Techniques Used: Mass Spectrometry

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    24) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    25) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    26) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    27) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    28) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    29) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    30) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).
    Figure Legend Snippet: Identification of the fusobacterial serine protease. Amino acid sequences of the putative serine protease open reading frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Red highlight indicates sequences identified by mass spectrometry of the 99 kDa serine protease of F. nucleatum ATCC 25586 (A), and of the 55 kDa serine protease of F. nucleatum ATCC 49256 (B).

    Techniques Used: Mass Spectrometry

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    31) Product Images from "Two mechanisms of oral malodor inhibition by zinc ions"

    Article Title: Two mechanisms of oral malodor inhibition by zinc ions

    Journal: Journal of Applied Oral Science

    doi: 10.1590/1678-7757-2017-0161

    Inhibitory effects of zinc ions on the growth of oral bacteria (mean ± SD). Streptococcus mutans JCM5705 (A), Streptococcus sobrinus (B), Streptococcus salivarius GTC0215 (C), Streptococcus anginosus FW73 (D), Porphyromonas gingivalis W83 (E), ATCC 33277 (F), FDC 381 (G), Prevotella intermedia ATCC 25611 (H), and Fusobacterium nucleatum ATCC 25586 (I)
    Figure Legend Snippet: Inhibitory effects of zinc ions on the growth of oral bacteria (mean ± SD). Streptococcus mutans JCM5705 (A), Streptococcus sobrinus (B), Streptococcus salivarius GTC0215 (C), Streptococcus anginosus FW73 (D), Porphyromonas gingivalis W83 (E), ATCC 33277 (F), FDC 381 (G), Prevotella intermedia ATCC 25611 (H), and Fusobacterium nucleatum ATCC 25586 (I)

    Techniques Used:

    32) Product Images from "Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria"

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.187.15.5330-5340.2005

    Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are
    Figure Legend Snippet: Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are

    Techniques Used: Sequencing

    33) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    34) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    35) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    36) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    37) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    38) Product Images from "Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria"

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.187.15.5330-5340.2005

    Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are
    Figure Legend Snippet: Amino acid sequence alignment of FadA and its paralogues. Highlighted in gray are the identical residues shared among FadA proteins. The sequences of two FadA paralogues, FN1529 from F. nucleatum ATCC 25586 and FNV2159 from F. nucleatum ATCC 49256, are

    Techniques Used: Sequencing

    39) Product Images from "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿"

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00166-10

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC
    Figure Legend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Techniques Used: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested
    Figure Legend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Techniques Used: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from
    Figure Legend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Techniques Used: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative
    Figure Legend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Techniques Used: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown
    Figure Legend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Techniques Used:

    40) Product Images from "Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells"

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/425421

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Techniques Used:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P
    Figure Legend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Techniques Used: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P
    Figure Legend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Techniques Used:

    Related Articles

    Produced:

    Article Title: Fusobacterium Genomics Using MinION and Illumina Sequencing Enables Genome Completion and Correction
    Article Snippet: .. In an attempt to create consistency in genomic builds, we rotated the start site of each chromosome to represent the MreC gene (gene FN1496) as it was originally the first gene of the F. nucleatum subsp. nucleatum ATCC 25586 genome build (NCBI GCA_000007325.1 ). highlights how long reads produced by MinION sequences are able to scaffold and effectively cover a bacterial genome. ..

    Expressing:

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells
    Article Snippet: .. F. nucleatum ATCC 25586 tended to increase the RANKL expression at 1 day but not at 3 days, and significantly (P < 0.05), despite discrete, upregulated the OPG expression after both 1 day and 3 days (Figures – ). .. Whereas CTSL had no effects on RANKL and OPG expressions (data not shown), CTSH caused a significant (P < 0.05) upregulation of the F. nucleatum -induced RANKL expression at 3 days and a significant (P < 0.05) downregulation of the F. nucleatum -induced OPG expression at 1 day and 3 days (Figures – ).

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    ATCC f nucleatum atcc 25586
    AT3 mammary tumor acceleration by F. <t>nucleatum</t> is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.
    F Nucleatum Atcc 25586, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    ATCC serine protease inhibitor pmsf
    <t>PMSF</t> inhibits growth of F. <t>nucleatum</t> but not of E. coli . (A) Growth of F. nucleatum 12230 (black line) is inhibited by PMSF (solid green line), but this inhibition is relieved by P. gingivalis supernatant (SN Pg) containing PMSF-resistant cysteine proteases (broken green line). (B) Growth of E. coli is not affected by PMSF, ruling out PMSF toxicity. *P
    Serine Protease Inhibitor Pmsf, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Article Snippet: Echoing this general patterns, a gel zymography assay with gelatin as substrate showed unaltered secretion of MMP-2 by cell lines AT3 (murine mammary tumor) or MDA-MB-231 (human breast cancer) incubated with F. nucleatum ATCC 23726 or with F. nucleatum ATCC 25586; by contrast, the secretion of MMP-9 was significantly and invariably increased when both cell lines where incubated with each of the two F. nucleatum strains (Fig. ).

    Techniques: Injection, Mouse Assay, Flow Cytometry, Two Tailed Test, MANN-WHITNEY, Expressing, Infection, Zymography, Multiple Displacement Amplification, Cell Culture

    Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Effects of exogenous indole on planktonic and biofilm cells of F. nucleatum ATCC 25586. (A) Relative amounts of planktonic and biofilm cells grown in the absence or presence of exogenous indole are shown. Bacteria were incubated for 48 h in 96-well PVC

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Incubation

    Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Characterization of purified protein encoded by fn1943 of F. nucleatum ATCC 25586. (A) SDS-PAGE analysis of recombinant Fn1943 of F. nucleatum ATCC 25586. Proteins were purified by affinity chromatography on glutathione-Sepharose 4B resin and digested

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Purification, SDS Page, Recombinant, Affinity Chromatography

    Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Genetic organization and transcriptional analyses of the tnaA region of F. nucleatum ATCC 25586. (A) Gene arrangements of the tnaA region in F. nucleatum ATCC 25586, E. coli K-12, and P. gingivalis W83. The DNA sequence of each region was obtained from

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Sequencing

    Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Expression levels of tnaA in several cell growth phases of F. nucleatum ATCC 25586. The OD 595 of cells incubated for 4, 8, 12, or 24 h for RNA extraction is indicated as an inset. The amounts of tnaA cDNA in each sample were analyzed by real-time quantitative

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques: Expressing, Incubation, RNA Extraction

    Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586 ▿

    doi: 10.1128/AEM.00166-10

    Figure Lengend Snippet: Effects of tryptophan on indole production and biofilm formation by F. nucleatum ATCC 25586. (A) Indole production by F. nucleatum ATCC 25586 cells grown in the absence or presence of tryptophan; (B) relative amounts of planktonic and biofilm cells grown

    Article Snippet: However, the transcription start site of the tna operon in F. nucleatum ATCC 25586 was 67 or 152 bp upstream from the initiation codon of tnaA (Fig. ), revealing that the region corresponding to tnaL in F. nucleatum was much shorter than that in E. coli.

    Techniques:

    (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Journal: Mediators of Inflammation

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    doi: 10.1155/2014/425421

    Figure Lengend Snippet: (a and b) RANKL expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG mRNA ratio in PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Article Snippet: F. nucleatum ATCC 25586 tended to increase the RANKL expression at 1 day but not at 3 days, and significantly (P < 0.05), despite discrete, upregulated the OPG expression after both 1 day and 3 days (Figures – ).

    Techniques: Expressing

    (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Journal: Mediators of Inflammation

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    doi: 10.1155/2014/425421

    Figure Lengend Snippet: (a and b) Synthesis of RANKL protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (a) and 3 days (b). (c and d) Synthesis of OPG protein in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (c) and 3 days (d). (e and f) RANKL/OPG protein ratio in supernatants of PDL cells stimulated by F. nucleatum ATCC 25586 and/or CTSH at 1 day (e) and 3 days (f). *Significant difference compared to other groups ( P

    Article Snippet: F. nucleatum ATCC 25586 tended to increase the RANKL expression at 1 day but not at 3 days, and significantly (P < 0.05), despite discrete, upregulated the OPG expression after both 1 day and 3 days (Figures – ).

    Techniques:

    (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Journal: Mediators of Inflammation

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    doi: 10.1155/2014/425421

    Figure Lengend Snippet: (a) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (b) COX2 expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (c) OPG expression in PDL cells stimulated by F. nucleatum ATCC 25586 over time. (d) OPG expression in PDL cells stimulated by various concentrations of F. nucleatum ATCC 25586 at 1 day. (e) COX2 expression in PDL cells stimulated by F. nucleatum ATCC 25586 (OD 0.025) in the presence or in the absence of anti-TLR2 or anti-TLR4 antibodies at 1 day. *Significant difference between groups ( P

    Article Snippet: F. nucleatum ATCC 25586 tended to increase the RANKL expression at 1 day but not at 3 days, and significantly (P < 0.05), despite discrete, upregulated the OPG expression after both 1 day and 3 days (Figures – ).

    Techniques: Expressing

    (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Journal: Mediators of Inflammation

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells

    doi: 10.1155/2014/425421

    Figure Lengend Snippet: (a) Synthesis of COX2 in lysates of PDL cells treated with F. nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL, 3%) and high (CTSH, 20%) magnitudes at 36 hours. (b and c) Production of PGE2 in supernatants of PDL cells treated with F. nucleatum ATCC 25586 and/or CTSL and CTSH at 1 day (b) and 3 days (c). *Significant difference compared to other groups ( P

    Article Snippet: F. nucleatum ATCC 25586 tended to increase the RANKL expression at 1 day but not at 3 days, and significantly (P < 0.05), despite discrete, upregulated the OPG expression after both 1 day and 3 days (Figures – ).

    Techniques:

    PMSF inhibits growth of F. nucleatum but not of E. coli . (A) Growth of F. nucleatum 12230 (black line) is inhibited by PMSF (solid green line), but this inhibition is relieved by P. gingivalis supernatant (SN Pg) containing PMSF-resistant cysteine proteases (broken green line). (B) Growth of E. coli is not affected by PMSF, ruling out PMSF toxicity. *P

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    doi: 10.1371/journal.pone.0111329

    Figure Lengend Snippet: PMSF inhibits growth of F. nucleatum but not of E. coli . (A) Growth of F. nucleatum 12230 (black line) is inhibited by PMSF (solid green line), but this inhibition is relieved by P. gingivalis supernatant (SN Pg) containing PMSF-resistant cysteine proteases (broken green line). (B) Growth of E. coli is not affected by PMSF, ruling out PMSF toxicity. *P

    Article Snippet: All the detected proteases were inhibited by the serine protease inhibitor PMSF (presented for F. nucleatum strains FDC 364, ATCC 25586, 12230 and ATCC 23726 in ).

    Techniques: Inhibition

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    doi: 10.1371/journal.pone.0111329

    Figure Lengend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Article Snippet: All the detected proteases were inhibited by the serine protease inhibitor PMSF (presented for F. nucleatum strains FDC 364, ATCC 25586, 12230 and ATCC 23726 in ).

    Techniques: Activity Assay, Molecular Weight