f nucleatum atcc 23726  (ATCC)


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    Name:
    Fusobacterium nucleatum subsp nucleatum VPI 4351 1210
    Description:

    Catalog Number:
    23726
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    Structured Review

    ATCC f nucleatum atcc 23726
    Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. <t>nucleatum</t> <t>ATCC</t> 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.

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    Images

    1) Product Images from " Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents"

    Article Title: Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents

    Journal: The Open Dentistry Journal

    doi: 10.2174/1874210601509010125

    Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.
    Figure Legend Snippet: Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.

    Techniques Used: Staining, IA

    CLSM images of biofilms on DBR disc surfaces. Biofilm formation of F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) on DBR discs. For each set, the image on the left was taken through a 20x objective (Scale bar, 50 μm); while the image on the right was taken through a 63x objective (Scale bar, 20 μm). Four random fields of view were examined for each sample and representative images are shown.
    Figure Legend Snippet: CLSM images of biofilms on DBR disc surfaces. Biofilm formation of F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) on DBR discs. For each set, the image on the left was taken through a 20x objective (Scale bar, 50 μm); while the image on the right was taken through a 63x objective (Scale bar, 20 μm). Four random fields of view were examined for each sample and representative images are shown.

    Techniques Used: Confocal Laser Scanning Microscopy, IA

    2) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Self-restriction of Fsp25586 is not efficient. Zymogram analysis of cell culture supernatant prepared from F. nucleatum ATCC 23726 carrying the pHS30 vector (A), or the pHSPROT plasmid expressing Fsp25586 (B).
    Figure Legend Snippet: Self-restriction of Fsp25586 is not efficient. Zymogram analysis of cell culture supernatant prepared from F. nucleatum ATCC 23726 carrying the pHS30 vector (A), or the pHSPROT plasmid expressing Fsp25586 (B).

    Techniques Used: Cell Culture, Plasmid Preparation, Expressing

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    3) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    4) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    5) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    6) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    7) Product Images from "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System"

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.12.6957-6962.2004

    pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.
    Figure Legend Snippet: pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.

    Techniques Used: Sequencing

    Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.
    Figure Legend Snippet: Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.

    Techniques Used: Transformation Assay, Plasmid Preparation, Isolation, Countercurrent Chromatography

    8) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    9) Product Images from " Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents"

    Article Title: Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents

    Journal: The Open Dentistry Journal

    doi: 10.2174/1874210601509010125

    Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.
    Figure Legend Snippet: Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.

    Techniques Used: Staining, IA

    CLSM images of biofilms on DBR disc surfaces. Biofilm formation of F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) on DBR discs. For each set, the image on the left was taken through a 20x objective (Scale bar, 50 μm); while the image on the right was taken through a 63x objective (Scale bar, 20 μm). Four random fields of view were examined for each sample and representative images are shown.
    Figure Legend Snippet: CLSM images of biofilms on DBR disc surfaces. Biofilm formation of F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) on DBR discs. For each set, the image on the left was taken through a 20x objective (Scale bar, 50 μm); while the image on the right was taken through a 63x objective (Scale bar, 20 μm). Four random fields of view were examined for each sample and representative images are shown.

    Techniques Used: Confocal Laser Scanning Microscopy, IA

    10) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Self-restriction of Fsp25586 is not efficient. Zymogram analysis of cell culture supernatant prepared from F. nucleatum ATCC 23726 carrying the pHS30 vector (A), or the pHSPROT plasmid expressing Fsp25586 (B).
    Figure Legend Snippet: Self-restriction of Fsp25586 is not efficient. Zymogram analysis of cell culture supernatant prepared from F. nucleatum ATCC 23726 carrying the pHS30 vector (A), or the pHSPROT plasmid expressing Fsp25586 (B).

    Techniques Used: Cell Culture, Plasmid Preparation, Expressing

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    11) Product Images from "Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease"

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111329

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.
    Figure Legend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Techniques Used: Activity Assay, Molecular Weight

    Self-restriction of Fsp25586 is not efficient. Zymogram analysis of cell culture supernatant prepared from F. nucleatum ATCC 23726 carrying the pHS30 vector (A), or the pHSPROT plasmid expressing Fsp25586 (B).
    Figure Legend Snippet: Self-restriction of Fsp25586 is not efficient. Zymogram analysis of cell culture supernatant prepared from F. nucleatum ATCC 23726 carrying the pHS30 vector (A), or the pHSPROT plasmid expressing Fsp25586 (B).

    Techniques Used: Cell Culture, Plasmid Preparation, Expressing

    Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.
    Figure Legend Snippet: Protease profiles of F. nucleatum growth medium supernatants on fibrinogen containing zymograms. M, Molecular weight markers. A, F. nucleatum ATCC 49256. B, F. nucleatum FDC 364. C, F. nucleatum ATCC 10953. D, F. nucleatum ATCC 25586. E, F. nucleatum ATCC 23726. F, F. nucleatum 12230. Arrows indicate proteolytic bands. Presented data are of representative zymograms.

    Techniques Used: Molecular Weight

    12) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    13) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    14) Product Images from "Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii"

    Article Title: Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii. Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

    Journal: MicrobiologyOpen

    doi: 10.1002/mbo3.444

    Quantitative coaggregation of (a) wild‐type ( WT ) Streptococcus gordonii strain V288 and the WT Fusobacterium nucleatum strain ATCC 23726, or the radD mutant derivative, at different phases of growth. (b) Quantitative coaggregation of WT S. gordonii strains ( DL 1, V288, ATCC 10558, and ATCC 51656) with WT F. nucleatum strain ATCC 23726, or the radD mutant derivative at exponential growth. Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p
    Figure Legend Snippet: Quantitative coaggregation of (a) wild‐type ( WT ) Streptococcus gordonii strain V288 and the WT Fusobacterium nucleatum strain ATCC 23726, or the radD mutant derivative, at different phases of growth. (b) Quantitative coaggregation of WT S. gordonii strains ( DL 1, V288, ATCC 10558, and ATCC 51656) with WT F. nucleatum strain ATCC 23726, or the radD mutant derivative at exponential growth. Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative coaggregation of wild‐type ( WT ) Streptococcus gordonii strain V288 with Fusobacterium nucleatum ATCC 23726 WT strain and OMP mutant derivatives ( radD , Fn254, cmpA , Fn1893, Fn2047, and aim1 ). Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p
    Figure Legend Snippet: Quantitative coaggregation of wild‐type ( WT ) Streptococcus gordonii strain V288 with Fusobacterium nucleatum ATCC 23726 WT strain and OMP mutant derivatives ( radD , Fn254, cmpA , Fn1893, Fn2047, and aim1 ). Data represent means and standard deviation of percent coaggregation of at least three independent experiments. * p

    Techniques Used: Mutagenesis, Standard Deviation

    15) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    16) Product Images from "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System"

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.12.6957-6962.2004

    pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.
    Figure Legend Snippet: pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.

    Techniques Used: Sequencing

    Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.
    Figure Legend Snippet: Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.

    Techniques Used: Transformation Assay, Plasmid Preparation, Isolation, Countercurrent Chromatography

    17) Product Images from "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System"

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.12.6957-6962.2004

    pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.
    Figure Legend Snippet: pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.

    Techniques Used: Sequencing

    Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.
    Figure Legend Snippet: Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.

    Techniques Used: Transformation Assay, Plasmid Preparation, Isolation, Countercurrent Chromatography

    18) Product Images from "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System"

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.12.6957-6962.2004

    pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.
    Figure Legend Snippet: pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.

    Techniques Used: Sequencing

    Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.
    Figure Legend Snippet: Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.

    Techniques Used: Transformation Assay, Plasmid Preparation, Isolation, Countercurrent Chromatography

    19) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    20) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    21) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    22) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    23) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    24) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    25) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    26) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    27) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    28) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    29) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    30) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA coding for the adhesin FadA ( 6 ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    31) Product Images from "Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein"

    Article Title: Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein

    Journal: Journal of dental research

    doi:

    Identification of Fap2 Homologs in F. nucleatum ATCC 23726
    Figure Legend Snippet: Identification of Fap2 Homologs in F. nucleatum ATCC 23726

    Techniques Used:

    Structural and transcriptional analyses of wild-type and aim less1 mutant strains. (A) Schematic illustration of mutagenesis: organization of aim 1 and flanking genes on the F. nucleatum ATCC 23726 wild-type and aim less1 mutant chromosome. Arrows indicate
    Figure Legend Snippet: Structural and transcriptional analyses of wild-type and aim less1 mutant strains. (A) Schematic illustration of mutagenesis: organization of aim 1 and flanking genes on the F. nucleatum ATCC 23726 wild-type and aim less1 mutant chromosome. Arrows indicate

    Techniques Used: Mutagenesis

    32) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    33) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    34) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    35) Product Images from "Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis"

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    Journal: International Journal of Oral Science

    doi: 10.1038/ijos.2016.27

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.
    Figure Legend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Techniques Used: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P
    Figure Legend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Techniques Used: Inhibition, Standard Deviation

    36) Product Images from "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System"

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.12.6957-6962.2004

    pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.
    Figure Legend Snippet: pKH9 axf-txf toxin-antitoxin system. (A) Stability of a pKH9 derivative containing axf-txf (pKH90) (triangles) versus that of a derivative lacking axf-txf (pORI9) (squares) in F. nucleatum ATCC 23726. Open symbols represent results of the first experiment, and filled symbols represent those of a second independent experiment. (B) Region of overlapping sequence between the axf and txf genes.

    Techniques Used: Sequencing

    Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.
    Figure Legend Snippet: Compatibility of pHS30 and pORI91. F. nucleatum ATCC 23726 harboring pHS30 was transformed with pORI91. Transformants were selected on media with clindamycin. Plasmid DNA isolated from representative transformants (T-1 and T-2) was examined by restriction enzyme digestion and compared with similarly digested pHS30 and pORI91 isolated from E. coli . DNA in lanes 1 was digested with KpnI to linearize pORI91. DNA in lanes 2 was digested with EcoRV to linearize pHS30. The open circular (OC), linear (L), and covalently closed circular (CCC) forms of pHS30 and pORI91 are indicated on the left and right, respectively.

    Techniques Used: Transformation Assay, Plasmid Preparation, Isolation, Countercurrent Chromatography

    37) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    38) Product Images from "Fusobacterium nucleatum Outer Membrane Proteins Fap2 and RadD Induce Cell Death in Human Lymphocytes ▿"

    Article Title: Fusobacterium nucleatum Outer Membrane Proteins Fap2 and RadD Induce Cell Death in Human Lymphocytes ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00567-10

    Jurkat cell death induction by F. nucleatum . Cell death rates were quantified based on the percentage of annexin V-FITC-positive Jurkat cells in samples incubated with F. nucleatum whole cells or membranes. F. nucleatum ATCC 23726 cells and membranes induced cell death in ∼75% and 41% of Jurkat cells, respectively, in the annexin V assay, and these numbers serve as the basis for the relative calculations presented. n ≥ 3. P ≤ 0.01 for all conditions compared to the wild-type control ( wt ).
    Figure Legend Snippet: Jurkat cell death induction by F. nucleatum . Cell death rates were quantified based on the percentage of annexin V-FITC-positive Jurkat cells in samples incubated with F. nucleatum whole cells or membranes. F. nucleatum ATCC 23726 cells and membranes induced cell death in ∼75% and 41% of Jurkat cells, respectively, in the annexin V assay, and these numbers serve as the basis for the relative calculations presented. n ≥ 3. P ≤ 0.01 for all conditions compared to the wild-type control ( wt ).

    Techniques Used: Incubation, Annexin V Assay

    39) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    40) Product Images from "Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC"

    Article Title: Forward Genetic Dissection of Biofilm Development by Fusobacterium nucleatum: Novel Functions of Cell Division Proteins FtsX and EnvC

    Journal: mBio

    doi: 10.1128/mBio.00360-18

    Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.
    Figure Legend Snippet: Roles of fusobacterial ftsX and envC in the development of monospecies and multispecies biofilms. (A) ftsX locus in the chromosome of F. nucleatum ATCC 23726, with envC adjacent to ppnK , which encodes an NAD+ kinase. Upstream of ftsX is fadA ); expression of genes in the ftsX locus and fadA appears to be controlled by individual promoters. (B) Map of the nonreplicative vector pCWU8 used to generate unmarked, in-frame gene deletion mutants in F. nucleatum . The kanamycin resistance cassette ( kan ), chloramphenicol/thiamphenicol resistance gene ( catP ), and galK are indicated. The multiple cloning sites (MCS) contain EcoRI, SacI, KpnI, BamHI, and SalI. (C) Fusobacterial strains were evaluated for their ability to form monospecies biofilm using crystal violet staining. (D) Interaction of fusobacteria and S. oralis So34 was determined by a standard coaggregation assay, with a radD mutant used as a negative control. (E) Three-species biofilms were analyzed by confocal laser scanning microscopy at a magnification of ×20, using 16S rRNA-oligonucleotide probes specific for F. nucleatum (red), A. oris (green), and S. oralis (blue); side and top views are presented. The results are representative of three independent experiments performed in triplicate.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Staining, Mutagenesis, Negative Control, Confocal Laser Scanning Microscopy

    Related Articles

    Electroporation:

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System
    Article Snippet: .. The 4,584-bp fragment released by digestion of pKH9 with XbaI was PCR amplified and cloned into the XbaI site of pBK-erm (Fig. ; Table ) to create pKH90, which confers clindamycin resistance on F. nucleatum ATCC 23726 transformants isolated after electroporation (Fig. ). .. A 2-kb fragment containing the pKH9 putative rep gene was amplified and used to construct the pORI9 plasmid (Fig. ).

    Amplification:

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis
    Article Snippet: .. For F. nucleatum ATCC 23726 and its mutant derivatives, a portion of the Fusobacterium -specific fomA gene was amplified with Fn-F (forward) 5′-AGTTGCTCCAGCTTGGAGACCAAAT-3′ and Fn-R (reverse) 5′-AAGTTTACTTTTGTTAAAGTTTGTAATCTTCC-3′ primers. ..

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System
    Article Snippet: .. The 4,584-bp fragment released by digestion of pKH9 with XbaI was PCR amplified and cloned into the XbaI site of pBK-erm (Fig. ; Table ) to create pKH90, which confers clindamycin resistance on F. nucleatum ATCC 23726 transformants isolated after electroporation (Fig. ). .. A 2-kb fragment containing the pKH9 putative rep gene was amplified and used to construct the pORI9 plasmid (Fig. ).

    Clone Assay:

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System
    Article Snippet: .. The 4,584-bp fragment released by digestion of pKH9 with XbaI was PCR amplified and cloned into the XbaI site of pBK-erm (Fig. ; Table ) to create pKH90, which confers clindamycin resistance on F. nucleatum ATCC 23726 transformants isolated after electroporation (Fig. ). .. A 2-kb fragment containing the pKH9 putative rep gene was amplified and used to construct the pORI9 plasmid (Fig. ).

    Mutagenesis:

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis
    Article Snippet: .. For F. nucleatum ATCC 23726 and its mutant derivatives, a portion of the Fusobacterium -specific fomA gene was amplified with Fn-F (forward) 5′-AGTTGCTCCAGCTTGGAGACCAAAT-3′ and Fn-R (reverse) 5′-AAGTTTACTTTTGTTAAAGTTTGTAATCTTCC-3′ primers. ..

    Isolation:

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System
    Article Snippet: .. The 4,584-bp fragment released by digestion of pKH9 with XbaI was PCR amplified and cloned into the XbaI site of pBK-erm (Fig. ; Table ) to create pKH90, which confers clindamycin resistance on F. nucleatum ATCC 23726 transformants isolated after electroporation (Fig. ). .. A 2-kb fragment containing the pKH9 putative rep gene was amplified and used to construct the pORI9 plasmid (Fig. ).

    other:

    Article Title: Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont, Desulfobulbus oralis
    Article Snippet: CFS from a type strain of F. nucleatum (ATCC 23726) also supported the growth of D. oralis , indicating its physiological dependence is not restricted to the strain it coenriched with, although we have not extended that analysis to additional strains or species.

    Polymerase Chain Reaction:

    Article Title: Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System
    Article Snippet: .. The 4,584-bp fragment released by digestion of pKH9 with XbaI was PCR amplified and cloned into the XbaI site of pBK-erm (Fig. ; Table ) to create pKH90, which confers clindamycin resistance on F. nucleatum ATCC 23726 transformants isolated after electroporation (Fig. ). .. A 2-kb fragment containing the pKH9 putative rep gene was amplified and used to construct the pORI9 plasmid (Fig. ).

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    ATCC f nucleatum
    Desulfobulbus sp. strain HOT041 ( D. oralis ) in coculture with Fusobacterium <t>nucleatum</t> and in pure culture. (A) FISH using fluorescent oligonucleotide probes specific for Deltaproteobacteria (green) and universal Bacteria (red). (B). Growth of Desulfobulbus sp. strain HOT041 and F. nucleatum in coculture monitored by species-specific qPCR (with error bars based on three replicates). (C and D) Scanning electron micrographs of the D. oralis isolate. The arrowheads point to membrane vesicles.
    F Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC serine protease inhibitor pmsf
    <t>PMSF</t> inhibits growth of F. <t>nucleatum</t> but not of E. coli . (A) Growth of F. nucleatum 12230 (black line) is inhibited by PMSF (solid green line), but this inhibition is relieved by P. gingivalis supernatant (SN Pg) containing PMSF-resistant cysteine proteases (broken green line). (B) Growth of E. coli is not affected by PMSF, ruling out PMSF toxicity. *P
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    illumina inc f nucleatum atcc 23726 subsp
    The bloodstream is an efficient route of oral F. <t>nucleatum</t> for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p
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    Desulfobulbus sp. strain HOT041 ( D. oralis ) in coculture with Fusobacterium nucleatum and in pure culture. (A) FISH using fluorescent oligonucleotide probes specific for Deltaproteobacteria (green) and universal Bacteria (red). (B). Growth of Desulfobulbus sp. strain HOT041 and F. nucleatum in coculture monitored by species-specific qPCR (with error bars based on three replicates). (C and D) Scanning electron micrographs of the D. oralis isolate. The arrowheads point to membrane vesicles.

    Journal: mBio

    Article Title: Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont, Desulfobulbus oralis

    doi: 10.1128/mBio.02061-17

    Figure Lengend Snippet: Desulfobulbus sp. strain HOT041 ( D. oralis ) in coculture with Fusobacterium nucleatum and in pure culture. (A) FISH using fluorescent oligonucleotide probes specific for Deltaproteobacteria (green) and universal Bacteria (red). (B). Growth of Desulfobulbus sp. strain HOT041 and F. nucleatum in coculture monitored by species-specific qPCR (with error bars based on three replicates). (C and D) Scanning electron micrographs of the D. oralis isolate. The arrowheads point to membrane vesicles.

    Article Snippet: CFS from a type strain of F. nucleatum (ATCC 23726) also supported the growth of D. oralis , indicating its physiological dependence is not restricted to the strain it coenriched with, although we have not extended that analysis to additional strains or species.

    Techniques: Fluorescence In Situ Hybridization, Real-time Polymerase Chain Reaction

    Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.

    Journal: The Open Dentistry Journal

    Article Title: Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents

    doi: 10.2174/1874210601509010125

    Figure Lengend Snippet: Biofilm formation on DBR disc surfaces. Representative images after crystal violet staining of biofilms formed on DBR discs by F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) For each set, the image on the left was a control DBR disc without biofilms growing on the surface, the image on the right was a DBR disc with biofilms growing on the surface. The experiment was performed in triplicate.

    Article Snippet: T. forsythia ATCC 43037, V. atypica PK 1910 and F. nucleatum ATCC 23726 were grown anaerobically overnight at 37°C in TF medium, TH medium containing 0.06% (w/v) lactic acid and Columbia broth (Difco, USA), respectively [ ].

    Techniques: Staining, IA

    CLSM images of biofilms on DBR disc surfaces. Biofilm formation of F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) on DBR discs. For each set, the image on the left was taken through a 20x objective (Scale bar, 50 μm); while the image on the right was taken through a 63x objective (Scale bar, 20 μm). Four random fields of view were examined for each sample and representative images are shown.

    Journal: The Open Dentistry Journal

    Article Title: Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents

    doi: 10.2174/1874210601509010125

    Figure Lengend Snippet: CLSM images of biofilms on DBR disc surfaces. Biofilm formation of F. nucleatum ATCC 23726 (A) T. forsythia ATCC 43037 (B) V. atypica PK 1910 (C) K. pneumoniae IA 565 (D) on DBR discs. For each set, the image on the left was taken through a 20x objective (Scale bar, 50 μm); while the image on the right was taken through a 63x objective (Scale bar, 20 μm). Four random fields of view were examined for each sample and representative images are shown.

    Article Snippet: T. forsythia ATCC 43037, V. atypica PK 1910 and F. nucleatum ATCC 23726 were grown anaerobically overnight at 37°C in TF medium, TH medium containing 0.06% (w/v) lactic acid and Columbia broth (Difco, USA), respectively [ ].

    Techniques: Confocal Laser Scanning Microscopy, IA

    PMSF inhibits growth of F. nucleatum but not of E. coli . (A) Growth of F. nucleatum 12230 (black line) is inhibited by PMSF (solid green line), but this inhibition is relieved by P. gingivalis supernatant (SN Pg) containing PMSF-resistant cysteine proteases (broken green line). (B) Growth of E. coli is not affected by PMSF, ruling out PMSF toxicity. *P

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    doi: 10.1371/journal.pone.0111329

    Figure Lengend Snippet: PMSF inhibits growth of F. nucleatum but not of E. coli . (A) Growth of F. nucleatum 12230 (black line) is inhibited by PMSF (solid green line), but this inhibition is relieved by P. gingivalis supernatant (SN Pg) containing PMSF-resistant cysteine proteases (broken green line). (B) Growth of E. coli is not affected by PMSF, ruling out PMSF toxicity. *P

    Article Snippet: All the detected proteases were inhibited by the serine protease inhibitor PMSF (presented for F. nucleatum strains FDC 364, ATCC 25586, 12230 and ATCC 23726 in ).

    Techniques: Inhibition

    PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

    doi: 10.1371/journal.pone.0111329

    Figure Lengend Snippet: PMSF inhibits the proteolytic activity of F. nucleatum . A, F. nucleatum FDC 364. B, F. nucleatum ATCC 25586. C, F. nucleatum 12230. D, F. nucleatum ATCC 23726. M, Molecular weight markers. Presented data are of representative zymograms.

    Article Snippet: All the detected proteases were inhibited by the serine protease inhibitor PMSF (presented for F. nucleatum strains FDC 364, ATCC 25586, 12230 and ATCC 23726 in ).

    Techniques: Activity Assay, Molecular Weight

    The bloodstream is an efficient route of oral F. nucleatum for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System

    doi: 10.3389/fcimb.2020.00400

    Figure Lengend Snippet: The bloodstream is an efficient route of oral F. nucleatum for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p

    Article Snippet: These results were repeated using a different F. nucleatum sub species, F. nucleatum ATCC 23726 subsp. nucleatum and a different mouse model, the CT26-luc colon cancer model in the BALB/cJ mouse ( ).

    Techniques: Mouse Assay, Staining, Labeling, Binding Assay, Mutagenesis, Negative Control, In Vivo, Tumor Implantation

    Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System

    doi: 10.3389/fcimb.2020.00400

    Figure Lengend Snippet: Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p

    Article Snippet: These results were repeated using a different F. nucleatum sub species, F. nucleatum ATCC 23726 subsp. nucleatum and a different mouse model, the CT26-luc colon cancer model in the BALB/cJ mouse ( ).

    Techniques: Stable Transfection, Transfection, Luciferase, Mouse Assay