f nucleatum atcc 10953  (ATCC)


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    Name:
    Fusobacterium nucleatum subsp polymorphum NCTC 10562
    Description:

    Catalog Number:
    10953
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    Structured Review

    ATCC f nucleatum atcc 10953
    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium <t>nucleatum</t> polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

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    Images

    1) Product Images from "The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine"

    Article Title: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

    Journal: BMC Microbiology

    doi: 10.1186/s12866-017-0967-9

    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay
    Figure Legend Snippet: The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Techniques Used: Activity Assay, Protein Extraction, Nucleic Acid Electrophoresis

    2) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    3) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    4) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    5) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    6) Product Images from "Quantitative Microbiological Study of Subgingival Plaque by Real-Time PCR Shows Correlation between Levels of Tannerella forsythensis and Fusobacterium spp."

    Article Title: Quantitative Microbiological Study of Subgingival Plaque by Real-Time PCR Shows Correlation between Levels of Tannerella forsythensis and Fusobacterium spp.

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.42.5.2255-2257.2004

    Amplification plots of genomic DNA from lysed cells. Serial dilutions of genomic DNA from F. nucleatum ATCC 10953 (A) or T. forsythensis ATCC 43037 (B) are shown. The log-transformed relative fluorescence [ΔRn (log)] was monitored as the increase in reporter dye intensity relative to that of the passive internal reference dye. The threshold fluorescence, or the level at which the threshold cycle was determined, is shown. The standard curves were generated from the amplification plots in the insets (correlation coefficients, 0.999 for F. nucleatum and 0.994 for T. forsythensis ). Ct is the cycle number at which the threshold fluorescence was reached.
    Figure Legend Snippet: Amplification plots of genomic DNA from lysed cells. Serial dilutions of genomic DNA from F. nucleatum ATCC 10953 (A) or T. forsythensis ATCC 43037 (B) are shown. The log-transformed relative fluorescence [ΔRn (log)] was monitored as the increase in reporter dye intensity relative to that of the passive internal reference dye. The threshold fluorescence, or the level at which the threshold cycle was determined, is shown. The standard curves were generated from the amplification plots in the insets (correlation coefficients, 0.999 for F. nucleatum and 0.994 for T. forsythensis ). Ct is the cycle number at which the threshold fluorescence was reached.

    Techniques Used: Amplification, Transformation Assay, Fluorescence, Generated

    7) Product Images from "Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria"

    Article Title: Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.187.15.5330-5340.2005

    DNA dot blot analysis of the fadA gene in different Fusobacterium species. 1, F. gonidiaformans DUMC CF65-1; 2, F. gonidiaformans DUMC CF63-1; 3, F. mortiferum ATCC 25557; 4, F. naviforme DUMC CF108-1; 5, F. nucleatum ATCC 10953; 6, F. nucleatum ATCC
    Figure Legend Snippet: DNA dot blot analysis of the fadA gene in different Fusobacterium species. 1, F. gonidiaformans DUMC CF65-1; 2, F. gonidiaformans DUMC CF63-1; 3, F. mortiferum ATCC 25557; 4, F. naviforme DUMC CF108-1; 5, F. nucleatum ATCC 10953; 6, F. nucleatum ATCC

    Techniques Used: Dot Blot

    8) Product Images from "The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine"

    Article Title: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

    Journal: BMC Microbiology

    doi: 10.1186/s12866-017-0967-9

    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay
    Figure Legend Snippet: The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Techniques Used: Activity Assay, Protein Extraction, Nucleic Acid Electrophoresis

    9) Product Images from "Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems †"

    Article Title: Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems †

    Journal: Journal of Bacteriology

    doi:

    Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with Eco RI digests (B) or pFN3 DNA with Eco RV digests (C). The Hin cII-digested pFN1 and Ase I-digested pFN3 probes were 32 P labeled (specific activity of 25 × 10 8 and 4.5 × 10 8 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.
    Figure Legend Snippet: Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with Eco RI digests (B) or pFN3 DNA with Eco RV digests (C). The Hin cII-digested pFN1 and Ase I-digested pFN3 probes were 32 P labeled (specific activity of 25 × 10 8 and 4.5 × 10 8 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.

    Techniques Used: Plasmid Preparation, Labeling, Activity Assay

    10) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    11) Product Images from "Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva "

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00345-09

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.
    Figure Legend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Techniques Used: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.
    Figure Legend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Techniques Used:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.
    Figure Legend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.
    Figure Legend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.
    Figure Legend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Techniques Used: Flow Cytometry

    12) Product Images from "Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva "

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00345-09

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.
    Figure Legend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Techniques Used: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.
    Figure Legend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Techniques Used:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.
    Figure Legend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.
    Figure Legend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.
    Figure Legend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Techniques Used: Flow Cytometry

    13) Product Images from "Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva "

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00345-09

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.
    Figure Legend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Techniques Used: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.
    Figure Legend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Techniques Used:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.
    Figure Legend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.
    Figure Legend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.
    Figure Legend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Techniques Used: Flow Cytometry

    14) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    15) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    16) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    17) Product Images from "Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva "

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00345-09

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.
    Figure Legend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Techniques Used: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.
    Figure Legend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Techniques Used:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.
    Figure Legend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.
    Figure Legend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.
    Figure Legend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Techniques Used: Flow Cytometry

    18) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    19) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    20) Product Images from "Quantitative Microbiological Study of Subgingival Plaque by Real-Time PCR Shows Correlation between Levels of Tannerella forsythensis and Fusobacterium spp."

    Article Title: Quantitative Microbiological Study of Subgingival Plaque by Real-Time PCR Shows Correlation between Levels of Tannerella forsythensis and Fusobacterium spp.

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.42.5.2255-2257.2004

    Amplification plots of genomic DNA from lysed cells. Serial dilutions of genomic DNA from F. nucleatum ATCC 10953 (A) or T. forsythensis ATCC 43037 (B) are shown. The log-transformed relative fluorescence [ΔRn (log)] was monitored as the increase in reporter dye intensity relative to that of the passive internal reference dye. The threshold fluorescence, or the level at which the threshold cycle was determined, is shown. The standard curves were generated from the amplification plots in the insets (correlation coefficients, 0.999 for F. nucleatum and 0.994 for T. forsythensis ). Ct is the cycle number at which the threshold fluorescence was reached.
    Figure Legend Snippet: Amplification plots of genomic DNA from lysed cells. Serial dilutions of genomic DNA from F. nucleatum ATCC 10953 (A) or T. forsythensis ATCC 43037 (B) are shown. The log-transformed relative fluorescence [ΔRn (log)] was monitored as the increase in reporter dye intensity relative to that of the passive internal reference dye. The threshold fluorescence, or the level at which the threshold cycle was determined, is shown. The standard curves were generated from the amplification plots in the insets (correlation coefficients, 0.999 for F. nucleatum and 0.994 for T. forsythensis ). Ct is the cycle number at which the threshold fluorescence was reached.

    Techniques Used: Amplification, Transformation Assay, Fluorescence, Generated

    21) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    22) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    23) Product Images from "Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems †"

    Article Title: Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems †

    Journal: Journal of Bacteriology

    doi:

    Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with Eco RI digests (B) or pFN3 DNA with Eco RV digests (C). The Hin cII-digested pFN1 and Ase I-digested pFN3 probes were 32 P labeled (specific activity of 25 × 10 8 and 4.5 × 10 8 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.
    Figure Legend Snippet: Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with Eco RI digests (B) or pFN3 DNA with Eco RV digests (C). The Hin cII-digested pFN1 and Ase I-digested pFN3 probes were 32 P labeled (specific activity of 25 × 10 8 and 4.5 × 10 8 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.

    Techniques Used: Plasmid Preparation, Labeling, Activity Assay

    24) Product Images from "The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine"

    Article Title: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

    Journal: BMC Microbiology

    doi: 10.1186/s12866-017-0967-9

    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay
    Figure Legend Snippet: The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Techniques Used: Activity Assay, Protein Extraction, Nucleic Acid Electrophoresis

    25) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    26) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    27) Product Images from "Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva "

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00345-09

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.
    Figure Legend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Techniques Used: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.
    Figure Legend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Techniques Used:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.
    Figure Legend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.
    Figure Legend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.
    Figure Legend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Techniques Used: Flow Cytometry

    28) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    29) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    30) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    31) Product Images from "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6"

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01311-15

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from
    Figure Legend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Techniques Used: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of
    Figure Legend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Techniques Used:

    32) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    33) Product Images from "Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development"

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    Journal: Anaerobe

    doi: 10.1016/j.anaerobe.2012.06.003

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum
    Figure Legend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Techniques Used:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.
    Figure Legend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Techniques Used: Co-Culture Assay

    34) Product Images from "Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems †"

    Article Title: Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems †

    Journal: Journal of Bacteriology

    doi:

    Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with Eco RI digests (B) or pFN3 DNA with Eco RV digests (C). The Hin cII-digested pFN1 and Ase I-digested pFN3 probes were 32 P labeled (specific activity of 25 × 10 8 and 4.5 × 10 8 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.
    Figure Legend Snippet: Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with Eco RI digests (B) or pFN3 DNA with Eco RV digests (C). The Hin cII-digested pFN1 and Ase I-digested pFN3 probes were 32 P labeled (specific activity of 25 × 10 8 and 4.5 × 10 8 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.

    Techniques Used: Plasmid Preparation, Labeling, Activity Assay

    35) Product Images from "Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva "

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00345-09

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.
    Figure Legend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Techniques Used: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.
    Figure Legend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Techniques Used:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.
    Figure Legend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.
    Figure Legend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Techniques Used: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.
    Figure Legend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Techniques Used: Flow Cytometry

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    Modification:

    Article Title: Ratiometric Imaging of Extracellular pH in Bacterial Biofilms with C-SNARF-4
    Article Snippet: .. Bifidobacterium dentium (DSM 20436T ), Fusobacterium nucleatum subsp. polymorphum (ATCC 10953T ), Neisseria subflava (DSM 17610T ), Porphyromonas gingivalis (ATCC 33277T ), Prevotella intermedia (CCUG 24041T ), and Veillonella parvula (CCUG 5123T ) were cultivated anaerobically on modified Columbia blood agar ( ) and transferred to a liquid plaque medium ( ) at 36.5°C until mid- to late exponential phase before experimental use. .. Actinomyces naeslundii AK6 and Streptococcus mitis SK24 were kindly provided by M. Kilian, Department of Biomedicine, Aarhus University, Denmark.

    Concentration Assay:

    Article Title: Quantitative Microbiological Study of Subgingival Plaque by Real-Time PCR Shows Correlation between Levels of Tannerella forsythensis and Fusobacterium spp.
    Article Snippet: .. Standard curves for each organism were plotted for each primer-probe set with the Ct values obtained by amplifying successive 10-fold dilutions of a known concentration of DNA extracted from bacterial cells containing 1.2 × 106 CFU (230 ng/μl) for F. nucleatum ATCC 10953 and 4.2 × 105 CFU (370 ng/μl) for T. forsythensis ATCC 43037 (Fig. ). ..

    other:

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development
    Article Snippet: As also shows, the presence of F. nucleatum ATCC 10953 showed a small but statistically significant stimulation of the ability of P. gingivalis ATCC 33277 to form biofilms as part of a community.

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development
    Article Snippet: For these experiments we first tested F. nucleatum ATCC 10953 since we previously identified a metabolic interaction occurring between this strain and P. gingivalis W50 [ ].

    Plasmid Preparation:

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6
    Article Snippet: .. The plasmid DNA was electroporated into F. nucleatum ATCC 10953 as described by Haake et al. ( ) and plated on selective medium containing thiamphenicol to obtain the corresponding Δ fadI derivative. .. The genomic DNA of the obtained colonies was analyzed by PCR for the presence of the catP gene and the absence of fadI .

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    ATCC f nucleatum atcc 10953
    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium <t>nucleatum</t> polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay
    F Nucleatum Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Journal: BMC Microbiology

    Article Title: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

    doi: 10.1186/s12866-017-0967-9

    Figure Lengend Snippet: The effect of the environmental conditions on the activity of H 2 S-producing enzymes was tested for Fusobacterium nucleatum polymorphum ATCC 10953. The bacteria were grown in different broths before protein extraction and 1D gel electrophoresis followed by in-gel activity assay

    Article Snippet: For F. nucleatum ATCC 10953, the influence of other environmental conditions were tested in TH broth buffered to pH 6, pH 7, pH 8 or TH broth, pH 7.8 supplemented with glutathione (2.5 mg/ml), sodium sulfide (0.46 mg/ml), 5% serum, 50% serum or 50% serum with hemin (10 ml/l).

    Techniques: Activity Assay, Protein Extraction, Nucleic Acid Electrophoresis

    Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Journal: Anaerobe

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    doi: 10.1016/j.anaerobe.2012.06.003

    Figure Lengend Snippet: Effect of serum on viability of A. oris (Ao), V. parvula (Vp), F. nucleatum ATCC 10953 (Fn) and P. gingivalis 381 (Pg). Planktonic growth of microorganisms was evaluated in their appropriate nutrient-rich medium supplemented with 10% native human serum

    Article Snippet: For these experiments we first tested F. nucleatum ATCC 10953 since we previously identified a metabolic interaction occurring between this strain and P. gingivalis W50 [ ].

    Techniques:

    Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Journal: Anaerobe

    Article Title: Strain-Specific Colonization Patterns and Serum Modulation of Multi-Species Oral Biofilm Development

    doi: 10.1016/j.anaerobe.2012.06.003

    Figure Lengend Snippet: Planktonic growth of F. nucleatum ATCC 10953 in salivary growth medium as a mono-culture or in co-culture with A. oris and V. parvula . Panel A shows planktonic growth curves. Data points represent measurements from three independent replicate experiments.

    Article Snippet: For these experiments we first tested F. nucleatum ATCC 10953 since we previously identified a metabolic interaction occurring between this strain and P. gingivalis W50 [ ].

    Techniques: Co-Culture Assay

    (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Journal: Infection and Immunity

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    doi: 10.1128/IAI.01311-15

    Figure Lengend Snippet: (A) Western immunoblot of FAD-I protein in whole-cell lysates of F. nucleatum ATCC 25586, ATCC 23726, ATCC 10953, ATCC 10953 Δ fadI , and ATCC 10953 Δ fadI /pFAD-I 23726 (ATCC 10953 Δ fadI with plasmid-based expression of FAD-I from

    Article Snippet: The plasmid DNA was electroporated into F. nucleatum ATCC 10953 as described by Haake et al. ( ) and plated on selective medium containing thiamphenicol to obtain the corresponding Δ fadI derivative.

    Techniques: Western Blot, Plasmid Preparation, Expressing

    Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Journal: Infection and Immunity

    Article Title: FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6

    doi: 10.1128/IAI.01311-15

    Figure Lengend Snippet: Induction of hBD-2 mRNA by live bacterial cells (A) and cell wall preparations (10 μg/ml cell wall F. nucleatum [Fn] ATCC 25586, ATCC 23726, and ATCC 10953 (B). The data presented are means ± standard deviations (SD) of the results of

    Article Snippet: The plasmid DNA was electroporated into F. nucleatum ATCC 10953 as described by Haake et al. ( ) and plated on selective medium containing thiamphenicol to obtain the corresponding Δ fadI derivative.

    Techniques: