Structured Review

Millipore f c cacl2
F C Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/f c cacl2/product/Millipore
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
f c cacl2 - by Bioz Stars, 2020-02
85/100 stars

Related Products / Commonly Used Together

klk7
f/c thermolysin
klk14
brij35

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Related Articles

Activation Assay:

Article Title: Tissue Kallikrein Inhibitors Based on the Sunflower Trypsin Inhibitor Scaffold – A Potential Therapeutic Intervention for Skin Diseases
Article Snippet: KLK7 and KLK14 were activated by addition of 1 mM f/c CaCl2 (Sigma, Dorset, U.K.), 0.05% v/v Brij35 (Sigma, Dorset, U.K.), 20 nM f/c thermolysin (Sigma, Dorset, U.K.) and incubated at 37°C for 1 hour. .. The negative controls (no KLK) for the thermolysin and lysyl endopeptidase activation mix confirmed that the two enzymes do not increase the RFU when mixed with Boc-VPR-AMC.

Concentration Assay:

Article Title: Tissue Kallikrein Inhibitors Based on the Sunflower Trypsin Inhibitor Scaffold – A Potential Therapeutic Intervention for Skin Diseases
Article Snippet: Enzyme activations by removal of N-terminal zymogen sequences Recombinant KLK7, KLK8 and KLK14 ( > 95% purity by SDS-PAGE, endotoxin < 0.1 ng/μg, R & D, Minneapolis, U.S.) stocks were diluted with 1xPBS to a concentration of 120 nM. .. KLK7 and KLK14 were activated by addition of 1 mM f/c CaCl2 (Sigma, Dorset, U.K.), 0.05% v/v Brij35 (Sigma, Dorset, U.K.), 20 nM f/c thermolysin (Sigma, Dorset, U.K.) and incubated at 37°C for 1 hour.

Incubation:

Article Title: Tissue Kallikrein Inhibitors Based on the Sunflower Trypsin Inhibitor Scaffold – A Potential Therapeutic Intervention for Skin Diseases
Article Snippet: .. KLK7 and KLK14 were activated by addition of 1 mM f/c CaCl2 (Sigma, Dorset, U.K.), 0.05% v/v Brij35 (Sigma, Dorset, U.K.), 20 nM f/c thermolysin (Sigma, Dorset, U.K.) and incubated at 37°C for 1 hour. ..

Recombinant:

Article Title: Tissue Kallikrein Inhibitors Based on the Sunflower Trypsin Inhibitor Scaffold – A Potential Therapeutic Intervention for Skin Diseases
Article Snippet: Enzyme activations by removal of N-terminal zymogen sequences Recombinant KLK7, KLK8 and KLK14 ( > 95% purity by SDS-PAGE, endotoxin < 0.1 ng/μg, R & D, Minneapolis, U.S.) stocks were diluted with 1xPBS to a concentration of 120 nM. .. KLK7 and KLK14 were activated by addition of 1 mM f/c CaCl2 (Sigma, Dorset, U.K.), 0.05% v/v Brij35 (Sigma, Dorset, U.K.), 20 nM f/c thermolysin (Sigma, Dorset, U.K.) and incubated at 37°C for 1 hour.

SDS Page:

Article Title: Tissue Kallikrein Inhibitors Based on the Sunflower Trypsin Inhibitor Scaffold – A Potential Therapeutic Intervention for Skin Diseases
Article Snippet: Enzyme activations by removal of N-terminal zymogen sequences Recombinant KLK7, KLK8 and KLK14 ( > 95% purity by SDS-PAGE, endotoxin < 0.1 ng/μg, R & D, Minneapolis, U.S.) stocks were diluted with 1xPBS to a concentration of 120 nM. .. KLK7 and KLK14 were activated by addition of 1 mM f/c CaCl2 (Sigma, Dorset, U.K.), 0.05% v/v Brij35 (Sigma, Dorset, U.K.), 20 nM f/c thermolysin (Sigma, Dorset, U.K.) and incubated at 37°C for 1 hour.

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  • 80
    Millipore tppe digestion buffer
    <t>TPPE</t> cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of <t>carboxymethylated</t> myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.
    Tppe Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tppe digestion buffer/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tppe digestion buffer - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    80
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    Image Search Results


    TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Journal: The Journal of Biological Chemistry

    Article Title: Isolation and Characterization of a Thionin Proprotein-processing Enzyme from Barley *

    doi: 10.1074/jbc.M115.647859

    Figure Lengend Snippet: TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Article Snippet: The reduced and cysteine-carboxymethylated protein samples were dialyzed against TPPE digestion buffer (25 m m MES, 100 m m NaCl, 10 m m CaCl2 , pH 6.5) using Amicon Ultra 10K ultrafiltration centrifugal filters (Millipore) according to the recommendations of the manufacturer.

    Techniques: Mass Spectrometry, Recombinant

    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques: