ez link amine peg3 biotin  (Thermo Fisher)


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    Name:
    EZ-Link Amine-PEG3-Biotin
    Description:
    Thermo Scientific EZ-Link Amine-PEG3-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. Features of EZ-Link Amine-PEG3-Biotin: • Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules • Medium length—spacer arm (total length added to target) is 22.9 angstroms Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond. We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications. Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry). Related Products EZ-Link Amine-PEG2-Biotin
    Catalog Number:
    21347
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Conjugate:
    Biotin
    Size:
    50 mg
    Category:
    Labeling & Detection Products, Labels & Labeling Kits, Aldehyde-, Ketone-, & Carboxylate-Reactive Labels
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher ez link amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Thermo Scientific EZ-Link Amine-PEG3-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. Features of EZ-Link Amine-PEG3-Biotin: • Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules • Medium length—spacer arm (total length added to target) is 22.9 angstroms Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond. We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications. Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry). Related Products EZ-Link Amine-PEG2-Biotin
    https://www.bioz.com/result/ez link amine peg3 biotin/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ez link amine peg3 biotin - by Bioz Stars, 2019-12
    79/100 stars

    Images

    1) Product Images from "PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond"

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw741

    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Figure Legend Snippet: Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Techniques Used: Magnetic Beads, Isolation, Incubation, Immunoprecipitation

    Related Articles

    Incubation:

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond
    Article Snippet: Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min. .. Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min.

    other:

    Article Title: Changes in Metabolic Chemical Reporter Structure Yield a Selective Probe of O-GlcNAc Modification
    Article Snippet: EZ-Link Amine PEG3 -Biotin (0.460 mg, 0.111 mmol) (Thermo Scientific) was added and reaction let stir for 18 h upon which time solvent was removed by vacuum.

    Chromatin Immunoprecipitation:

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond
    Article Snippet: Final purified DNA libraries were sequenced by the High-Throughput Genomics Core within the University of Utah Huntsman Cancer Institute using the Illumina HiSeq 2000 platform. .. Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min. .. These beads were selected as they have the same size and core material composition as the protein G coated magnetic beads utilized in the Active Motif ChIP-exo Kit.

    Magnetic Beads:

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond
    Article Snippet: Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min. .. For each of the two replicates, the input sample (obtained as discussed above) was combined with 300 μl of 0.1 M EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) along with the pre-functionalized biotinylated magnetics beads and incubated in 10 ml 0.1 mM MES buffer (pH 5.0) for 3 h at room temperature on a mechanical rotator.

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  • 79
    Thermo Fisher ez link amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Ez Link Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link amine peg3 biotin/product/Thermo Fisher
    Average 79 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ez link amine peg3 biotin - by Bioz Stars, 2019-12
    79/100 stars
      Buy from Supplier

    Image Search Results


    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Journal: Nucleic Acids Research

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    doi: 10.1093/nar/gkw741

    Figure Lengend Snippet: Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Article Snippet: Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min.

    Techniques: Magnetic Beads, Isolation, Incubation, Immunoprecipitation