ez dna methylation kit (Zymo Research)
Zymo Research is a verified supplier 
Zymo Research manufactures this product 
99
|
Name:
EZ DNA Methylation Gold Kit
Description:
The EZ DNA Methylation Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA This allows for the denaturation and bisulfite conversion steps to be consolidated into one step leading to a much reduced incubation time Recovered bisulfite converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion sequencing microarrays etc
Catalog Number:
d5006
Price:
None
Category:
Life Science Reagents and Media
Applications:
Bisulfite Conversion
Size:
50 units
|
Buy from Supplier |
Structured Review
Zymo Research
ez dna methylation kit
The EZ DNA Methylation Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA This allows for the denaturation and bisulfite conversion steps to be consolidated into one step leading to a much reduced incubation time Recovered bisulfite converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion sequencing microarrays etc
https://www.bioz.com/result/ez dna methylation kit/product/Zymo Research
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
The EZ DNA Methylation Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA This allows for the denaturation and bisulfite conversion steps to be consolidated into one step leading to a much reduced incubation time Recovered bisulfite converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion sequencing microarrays etc
https://www.bioz.com/result/ez dna methylation kit/product/Zymo Research
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ez dna methylation kit - by Bioz Stars,
2021-03
99/100 stars
Images
Related Articles
Methylation:Article Title: High Fractional Occupancy of a Tandem Maf Recognition Element and Its Role in Long-Range β-Globin Gene Regulation Article Snippet: .. M.CviPI-methylated DNA was subjected to bisulfite conversion using a Zymo Research Article Title: A donor-specific epigenetic classifier for acute graft-versus-host disease severity in hematopoietic stem cell transplantation Article Snippet: .. Measurement of relative DNA methylation levels using MethyLight Genomic DNA was bisulfite-converted using an Article Title: Transcription of intragenic CpG islands influences spatiotemporal host gene pre-mRNA processing Article Snippet: DNA extraction and bisulfite sequencingGenomic DNA (gDNA) was extracted form pelleted cells using DNeasy Blood and Tissue Kit (Qiagen, 69504). .. 500 ng of gDNA were bisulfite converted using Article Title: Auxin‐activated MdARF5 induces the expression of ethylene biosynthetic genes to initiate apple fruit ripening Article Snippet: The intensity of the PCR product shift was measured using the c Series Capture Software. .. A BSP (bisulfite sequence PCR) assay was performed using the Article Title: DNMT1 maintains the methylation of miR‐152‐3p to regulate TMSB10 expression, thereby affecting the biological characteristics of colorectal cancer cells. DNMT1 maintains the methylation of miR‐152‐3p to regulate TMSB10 expression, thereby affecting the biological characteristics of colorectal cancer cells Article Snippet: The gray value of each band was analyzed via ImageJ software. .. 2.7 Methylation‐specific PCR ( MSP )Genomic DNA was processed with Article Title: Recurrent Chromosomal Copy Number Alterations in Sporadic Chordomas Article Snippet: .. CDKN2A and PTEN Promoter Methylation Specific PCR Purified genomic DNA from chordoma cases were bisulfite treated using the Zymo Research Article Title: EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma Article Snippet: Initially, glioma DNA was extracted using BGenomic DNA Extraction Kit (Beijing Tiangen Biotech, Beijing, China), DNA concentration and purity were determined with ultraviolet spectrophotometry and stored in −80°C refrigerator for later use. .. MS-PCR was then conducted with the Pyrosequencing Assay:Article Title: Epigenetic deregulation of GATA3 in neuroblastoma is associated with increased GATA3 protein expression and with poor outcomes Article Snippet: GATA3 transient silencing Cells were transfected with siRNAs (50 nM) against GATA3 , or a non-targeting siRNA negative control (Supplementary Table ; all synthesised by Sigma), using Lipofectamine 2000 transfection reagent (Invitrogen) and harvested after 72 hrs. .. Pyrosequencing assay of GATA3 DNA methylation DNA was bisulfite converted ( DNA Methylation Assay:Article Title: Epigenetic deregulation of GATA3 in neuroblastoma is associated with increased GATA3 protein expression and with poor outcomes Article Snippet: GATA3 transient silencing Cells were transfected with siRNAs (50 nM) against GATA3 , or a non-targeting siRNA negative control (Supplementary Table ; all synthesised by Sigma), using Lipofectamine 2000 transfection reagent (Invitrogen) and harvested after 72 hrs. .. Pyrosequencing assay of GATA3 DNA methylation DNA was bisulfite converted ( Article Title: A donor-specific epigenetic classifier for acute graft-versus-host disease severity in hematopoietic stem cell transplantation Article Snippet: .. Measurement of relative DNA methylation levels using MethyLight Genomic DNA was bisulfite-converted using an Amplification:Article Title: Epigenetic deregulation of GATA3 in neuroblastoma is associated with increased GATA3 protein expression and with poor outcomes Article Snippet: GATA3 transient silencing Cells were transfected with siRNAs (50 nM) against GATA3 , or a non-targeting siRNA negative control (Supplementary Table ; all synthesised by Sigma), using Lipofectamine 2000 transfection reagent (Invitrogen) and harvested after 72 hrs. .. Pyrosequencing assay of GATA3 DNA methylation DNA was bisulfite converted ( Polymerase Chain Reaction:Article Title: Epigenetic deregulation of GATA3 in neuroblastoma is associated with increased GATA3 protein expression and with poor outcomes Article Snippet: GATA3 transient silencing Cells were transfected with siRNAs (50 nM) against GATA3 , or a non-targeting siRNA negative control (Supplementary Table ; all synthesised by Sigma), using Lipofectamine 2000 transfection reagent (Invitrogen) and harvested after 72 hrs. .. Pyrosequencing assay of GATA3 DNA methylation DNA was bisulfite converted ( Article Title: Auxin‐activated MdARF5 induces the expression of ethylene biosynthetic genes to initiate apple fruit ripening Article Snippet: The intensity of the PCR product shift was measured using the c Series Capture Software. .. A BSP (bisulfite sequence PCR) assay was performed using the Article Title: DNMT1 maintains the methylation of miR‐152‐3p to regulate TMSB10 expression, thereby affecting the biological characteristics of colorectal cancer cells. DNMT1 maintains the methylation of miR‐152‐3p to regulate TMSB10 expression, thereby affecting the biological characteristics of colorectal cancer cells Article Snippet: The gray value of each band was analyzed via ImageJ software. .. 2.7 Methylation‐specific PCR ( MSP )Genomic DNA was processed with Article Title: Recurrent Chromosomal Copy Number Alterations in Sporadic Chordomas Article Snippet: .. CDKN2A and PTEN Promoter Methylation Specific PCR Purified genomic DNA from chordoma cases were bisulfite treated using the Zymo Research Sequencing:Article Title: Auxin‐activated MdARF5 induces the expression of ethylene biosynthetic genes to initiate apple fruit ripening Article Snippet: The intensity of the PCR product shift was measured using the c Series Capture Software. .. A BSP (bisulfite sequence PCR) assay was performed using the Purification:Article Title: Recurrent Chromosomal Copy Number Alterations in Sporadic Chordomas Article Snippet: .. CDKN2A and PTEN Promoter Methylation Specific PCR Purified genomic DNA from chordoma cases were bisulfite treated using the Zymo Research |
