expression vector pcmv lacz  (Thermo Fisher)


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    Thermo Fisher expression vector pcmv lacz
    Expression Vector Pcmv Lacz, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pcmv lacz/product/Thermo Fisher
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    expression vector pcmv lacz - by Bioz Stars, 2020-09
    85/100 stars

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    Related Articles

    Dominant Negative Mutation:

    Article Title: Sensitization of TRAIL-resistant LNCaP cells by resveratrol (3, 4', 5 tri-hydroxystilbene): molecular mechanisms and therapeutic potential
    Article Snippet: .. Cells were transfected with expression constructs encoding dominant negative FADD (DN-FADD) or empty vector in the presence of an expression vector pCMV-LacZ (Invitrogen life technologies) expressing β-galactosidase. ..

    Transfection:

    Article Title: Resveratrol Induces Growth Arrest and Apoptosis through Activation of FOXO Transcription Factors in Prostate Cancer Cells
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Resveratrol Inhibits Growth of Orthotopic Pancreatic Tumors through Activation of FOXO Transcription Factors
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE ) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Sensitization of TRAIL-resistant LNCaP cells by resveratrol (3, 4', 5 tri-hydroxystilbene): molecular mechanisms and therapeutic potential
    Article Snippet: .. Cells were transfected with expression constructs encoding dominant negative FADD (DN-FADD) or empty vector in the presence of an expression vector pCMV-LacZ (Invitrogen life technologies) expressing β-galactosidase. ..

    Mutagenesis:

    Article Title: Resveratrol Induces Growth Arrest and Apoptosis through Activation of FOXO Transcription Factors in Prostate Cancer Cells
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Resveratrol Inhibits Growth of Orthotopic Pancreatic Tumors through Activation of FOXO Transcription Factors
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE ) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Construct:

    Article Title: Resveratrol Induces Growth Arrest and Apoptosis through Activation of FOXO Transcription Factors in Prostate Cancer Cells
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Resveratrol Inhibits Growth of Orthotopic Pancreatic Tumors through Activation of FOXO Transcription Factors
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE ) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Sensitization of TRAIL-resistant LNCaP cells by resveratrol (3, 4', 5 tri-hydroxystilbene): molecular mechanisms and therapeutic potential
    Article Snippet: .. Cells were transfected with expression constructs encoding dominant negative FADD (DN-FADD) or empty vector in the presence of an expression vector pCMV-LacZ (Invitrogen life technologies) expressing β-galactosidase. ..

    Expressing:

    Article Title: Resveratrol Induces Growth Arrest and Apoptosis through Activation of FOXO Transcription Factors in Prostate Cancer Cells
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Resveratrol Inhibits Growth of Orthotopic Pancreatic Tumors through Activation of FOXO Transcription Factors
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE ) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Sensitization of TRAIL-resistant LNCaP cells by resveratrol (3, 4', 5 tri-hydroxystilbene): molecular mechanisms and therapeutic potential
    Article Snippet: .. Cells were transfected with expression constructs encoding dominant negative FADD (DN-FADD) or empty vector in the presence of an expression vector pCMV-LacZ (Invitrogen life technologies) expressing β-galactosidase. ..

    Plasmid Preparation:

    Article Title: Resveratrol Induces Growth Arrest and Apoptosis through Activation of FOXO Transcription Factors in Prostate Cancer Cells
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Resveratrol Inhibits Growth of Orthotopic Pancreatic Tumors through Activation of FOXO Transcription Factors
    Article Snippet: .. Cells were transfected with expression constructs encoding wild type PTEN (pSG5L-HA-PTENwt), mutant PTEN (pSG5L-HA-PTEN-G 129E and pSGL5-HA-PTEN-G 129R), wild type-AKT (pUSE-WT-AKT), constitutively active-AKT (pUSE-CA-AKT), dominant negative-AKT (pUSE-DN-AKT), or the corresponding empty vectors (pSG5L or pUSE ) in the presence of an expression vector pCMV-LacZ (Invitrogen Life Technologies) expressing β-galactosidase. ..

    Article Title: Sensitization of TRAIL-resistant LNCaP cells by resveratrol (3, 4', 5 tri-hydroxystilbene): molecular mechanisms and therapeutic potential
    Article Snippet: .. Cells were transfected with expression constructs encoding dominant negative FADD (DN-FADD) or empty vector in the presence of an expression vector pCMV-LacZ (Invitrogen life technologies) expressing β-galactosidase. ..

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    Thermo Fisher pcmv lacz vector
    CDK9–cyclin T1 complex regulates the recruitment of ATF4 to the CHOP promoter under ER stress. A, HEK293T cells were co-transfected with firefly luciferase reporter plasmid containing three repeats of the C/EBP–ATF composite (−303/−292) of the mouse CHOP gene, <t>pCMV-LacZ,</t> and control or human ATF4 expression plasmid. 4 h after transfection; cells were incubated in DMEM containing 10% fetal bovine serum with DMSO ( Veh ) or CDK9 inhibitors ( CDK9i ) such as 300 n m flavopiridol or 30 μ m CAY10574 for 24 h. The results are expressed as luciferase ( Luc )/β-gal units of induction ( n -fold) over the control value for each construct. One-way ANOVA with a Student-Newman post hoc test was used for comparison between vehicle, flavopiridol, and CAY10574-treated cells ( n = 6). B, ChIP analysis of SFA-induced ATF4 recruitment to the C/EBP-ATF composite of the CHOP promoter. VSMCs were pretreated with 10 μ m flavopiridol (CDK9i) for 2 h, and then treated with BSA ( Veh ) or 250 μ m C18:0 with or without 10 μ m CDK9i. After 6 h, chromatin fractions of VSMCs were prepared. ChIP was performed with rabbit control IgG or ATF4 antibodies. Purified immunoprecipitated DNA was analyzed by qRT-PCR with primers for the C/EBP–ATF composite of the CHOP promoter. The results are expressed as the percentage of antibody binding versus the amount of PCR product obtained using a standardized aliquot of input chromatin (% input) ( n = 3). One-way ANOVA with a Student-Newman post hoc test was used for statistical analysis. *, p
    Pcmv Lacz Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv lacz vector/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pcmv lacz vector - by Bioz Stars, 2020-09
    93/100 stars
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    CDK9–cyclin T1 complex regulates the recruitment of ATF4 to the CHOP promoter under ER stress. A, HEK293T cells were co-transfected with firefly luciferase reporter plasmid containing three repeats of the C/EBP–ATF composite (−303/−292) of the mouse CHOP gene, pCMV-LacZ, and control or human ATF4 expression plasmid. 4 h after transfection; cells were incubated in DMEM containing 10% fetal bovine serum with DMSO ( Veh ) or CDK9 inhibitors ( CDK9i ) such as 300 n m flavopiridol or 30 μ m CAY10574 for 24 h. The results are expressed as luciferase ( Luc )/β-gal units of induction ( n -fold) over the control value for each construct. One-way ANOVA with a Student-Newman post hoc test was used for comparison between vehicle, flavopiridol, and CAY10574-treated cells ( n = 6). B, ChIP analysis of SFA-induced ATF4 recruitment to the C/EBP-ATF composite of the CHOP promoter. VSMCs were pretreated with 10 μ m flavopiridol (CDK9i) for 2 h, and then treated with BSA ( Veh ) or 250 μ m C18:0 with or without 10 μ m CDK9i. After 6 h, chromatin fractions of VSMCs were prepared. ChIP was performed with rabbit control IgG or ATF4 antibodies. Purified immunoprecipitated DNA was analyzed by qRT-PCR with primers for the C/EBP–ATF composite of the CHOP promoter. The results are expressed as the percentage of antibody binding versus the amount of PCR product obtained using a standardized aliquot of input chromatin (% input) ( n = 3). One-way ANOVA with a Student-Newman post hoc test was used for statistical analysis. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: The CDK9–cyclin T1 complex mediates saturated fatty acid–induced vascular calcification by inducing expression of the transcription factor CHOP

    doi: 10.1074/jbc.RA118.004706

    Figure Lengend Snippet: CDK9–cyclin T1 complex regulates the recruitment of ATF4 to the CHOP promoter under ER stress. A, HEK293T cells were co-transfected with firefly luciferase reporter plasmid containing three repeats of the C/EBP–ATF composite (−303/−292) of the mouse CHOP gene, pCMV-LacZ, and control or human ATF4 expression plasmid. 4 h after transfection; cells were incubated in DMEM containing 10% fetal bovine serum with DMSO ( Veh ) or CDK9 inhibitors ( CDK9i ) such as 300 n m flavopiridol or 30 μ m CAY10574 for 24 h. The results are expressed as luciferase ( Luc )/β-gal units of induction ( n -fold) over the control value for each construct. One-way ANOVA with a Student-Newman post hoc test was used for comparison between vehicle, flavopiridol, and CAY10574-treated cells ( n = 6). B, ChIP analysis of SFA-induced ATF4 recruitment to the C/EBP-ATF composite of the CHOP promoter. VSMCs were pretreated with 10 μ m flavopiridol (CDK9i) for 2 h, and then treated with BSA ( Veh ) or 250 μ m C18:0 with or without 10 μ m CDK9i. After 6 h, chromatin fractions of VSMCs were prepared. ChIP was performed with rabbit control IgG or ATF4 antibodies. Purified immunoprecipitated DNA was analyzed by qRT-PCR with primers for the C/EBP–ATF composite of the CHOP promoter. The results are expressed as the percentage of antibody binding versus the amount of PCR product obtained using a standardized aliquot of input chromatin (% input) ( n = 3). One-way ANOVA with a Student-Newman post hoc test was used for statistical analysis. *, p

    Article Snippet: Cells were transfected with 200 ng of firefly luciferase reporter plasmids (pGL3, Promega), 50 ng of expression plasmid (FLAG-Atf4-pcDNA3.1), and 100 ng of pCMV-LacZ vector using Turbofect (ThermoFisher Scientific).

    Techniques: Transfection, Luciferase, Plasmid Preparation, Expressing, Incubation, Construct, Chromatin Immunoprecipitation, Purification, Immunoprecipitation, Quantitative RT-PCR, Binding Assay, Polymerase Chain Reaction