Article Title: Picornavirus Modification of a Host mRNA Decay Protein
Figure Lengend Snippet: Cleavage of AUF1 during poliovirus, human rhinovirus 14, or human rhinovirus 16 infection. HeLa cells were infected with poliovirus (A) (lanes 3 to 7), human rhinovirus 14 (B) (lanes 3 to 11), or human rhinovirus 16 (C) (lanes 3 to 11), and NP-40 lysates were generated at different times after infection. Lysates from mock-infected cells (M0 and M6.5 or M16, lanes 1 and 2) were generated at 0 hpi or at 6.5 hpi (for poliovirus) or 16 hpi (for human rhinovirus). Lysates were subjected to SDS-PAGE and Western blot analysis using anti-AUF1 rabbit polyclonal antibody and antinucleolin rabbit polyclonal antibody (as a loading control) to visualize proteins. For size comparison, a molecular mass marker was included (left side of the autoradiograph of the gel). The electrophoretic mobilities of endogenous AUF1, cleaved and uncleaved, and nucleolin are indicated by the bracket and arrow, respectively, on the right.
Article Snippet: Cellular extracts were subjected to SDS-PAGE, and proteins were electroblotted to a PVDF membrane that was then probed using anti-AUF1 (Millipore) and antinucleolin (Abcam) antibodies (with expression levels of nucleolin serving as a loading control).
Techniques: Infection, Generated, SDS Page, Western Blot, Marker, Autoradiography