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Roche expand long template pcr system kit
Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
Expand Long Template Pcr System Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy"

Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy

Journal: BioMed Research International

doi: 10.1155/2014/256245

Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
Figure Legend Snippet: Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

Techniques Used: Polymerase Chain Reaction, Marker

Related Articles

Clone Assay:

Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
Article Snippet: .. Yeast DNA was isolated from strain BY4743 as described (Sambrook and Russell, ); a 5.8 kb DNA fragment encoding the RPO41 gene (Masters et al. , ) was amplified from yeast chromosomal DNA using an Expand Long Template PCR System kit (Roche Applied Science), subcloned into pT7Blue vector, using a Perfectly Blunt cloning kit (EMD Bioscience/Novagen), and recloned into pRS316 and pRS315 yeast shuffling vectors (centromeric plasmids that exist as a single copy in the cell and thus ensure the lack of overexpression; Sikorski and Hieter, ) to produce pRS316–Rpo41p and pRS315–Rpo41p, respectively. .. Competent cells were prepared and transfected with the shuffling constructs as described in BD Yeastmaker Yeast Transformation System 2 User Manual (BD Biosciences).

Amplification:

Article Title: O-Linked Glycosylation of the PilA Pilin Protein of Francisella tularensis: Identification of the Endogenous Protein-Targeting Oligosaccharyltransferase and Characterization of the Native Oligosaccharide ▿: Identification of the Endogenous Protein-Targeting Oligosaccharyltransferase and Characterization of the Native Oligosaccharide ▿ †
Article Snippet: .. The gonococcal gene cluster containing the pglF , pglB , pglC , and pglD genes and upstream DNA were amplified by PCR from strain N400 using Expand Long Template PCR system kit (Roche) and the primers FE1175 and FE1170. .. The PCR product was cloned into TOPO vector pCRII-TOPO, released by the unique restriction sites BamHI and SphI, and then subcloned into the pACYC184 vector digested with BamHI and SphI.

Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy
Article Snippet: .. PCR Long-Range On absence of amplification of POLH exon 10, long PCR was performed using the Expand Long Template PCR System Kit (Expand Long Range dNTPack 700 units/μ L Roche). ..

Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
Article Snippet: .. Yeast DNA was isolated from strain BY4743 as described (Sambrook and Russell, ); a 5.8 kb DNA fragment encoding the RPO41 gene (Masters et al. , ) was amplified from yeast chromosomal DNA using an Expand Long Template PCR System kit (Roche Applied Science), subcloned into pT7Blue vector, using a Perfectly Blunt cloning kit (EMD Bioscience/Novagen), and recloned into pRS316 and pRS315 yeast shuffling vectors (centromeric plasmids that exist as a single copy in the cell and thus ensure the lack of overexpression; Sikorski and Hieter, ) to produce pRS316–Rpo41p and pRS315–Rpo41p, respectively. .. Competent cells were prepared and transfected with the shuffling constructs as described in BD Yeastmaker Yeast Transformation System 2 User Manual (BD Biosciences).

Isolation:

Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
Article Snippet: .. Yeast DNA was isolated from strain BY4743 as described (Sambrook and Russell, ); a 5.8 kb DNA fragment encoding the RPO41 gene (Masters et al. , ) was amplified from yeast chromosomal DNA using an Expand Long Template PCR System kit (Roche Applied Science), subcloned into pT7Blue vector, using a Perfectly Blunt cloning kit (EMD Bioscience/Novagen), and recloned into pRS316 and pRS315 yeast shuffling vectors (centromeric plasmids that exist as a single copy in the cell and thus ensure the lack of overexpression; Sikorski and Hieter, ) to produce pRS316–Rpo41p and pRS315–Rpo41p, respectively. .. Competent cells were prepared and transfected with the shuffling constructs as described in BD Yeastmaker Yeast Transformation System 2 User Manual (BD Biosciences).

Polymerase Chain Reaction:

Article Title: O-Linked Glycosylation of the PilA Pilin Protein of Francisella tularensis: Identification of the Endogenous Protein-Targeting Oligosaccharyltransferase and Characterization of the Native Oligosaccharide ▿: Identification of the Endogenous Protein-Targeting Oligosaccharyltransferase and Characterization of the Native Oligosaccharide ▿ †
Article Snippet: .. The gonococcal gene cluster containing the pglF , pglB , pglC , and pglD genes and upstream DNA were amplified by PCR from strain N400 using Expand Long Template PCR system kit (Roche) and the primers FE1175 and FE1170. .. The PCR product was cloned into TOPO vector pCRII-TOPO, released by the unique restriction sites BamHI and SphI, and then subcloned into the pACYC184 vector digested with BamHI and SphI.

Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy
Article Snippet: .. PCR Long-Range On absence of amplification of POLH exon 10, long PCR was performed using the Expand Long Template PCR System Kit (Expand Long Range dNTPack 700 units/μ L Roche). ..

Article Title: Endosymbiotic Gene Transfer in Tertiary Plastid-Containing Dinoflagellates
Article Snippet: .. The optimized PCR conditions were determined to be 94°C for 2 min, 39 cycles of 94°C for 15 s, 42°C for 30 s, 72°C for 5 min, and 72°C for 6 min, while the long-range PCR conditions were optimized at 92°C for 2 min, 34 cycles of 94°C for 10 s, 45°C for 15 s, 68°C for 20 min, and 68°C for 7 min using buffer 3 from an Expand Long Template PCR System kit (Roche Applied Science, Indianapolis, IN). .. The amplified SL cDNAs of D. baltica and K. foliaceum were sequenced using massively parallel GS-FLX DNA pyrosequencing (Roche 454 Life Sciences, Branford, CT), which was carried out at the Génome Québec Innovation Centre.

Article Title: A unique MSH2 exon 8 deletion accounts for a major portion of all mismatch repair gene mutations in Lynch syndrome families of Sardinian origin
Article Snippet: .. The Expand Long Template PCR system kit (Roche Diagnostics GmbH) was used according to the manufacturer's protocol. ..

Article Title: In Silico Prediction and Validation of Gfap as an miR-3099 Target in Mouse Brain
Article Snippet: .. The PCR was carried out using Expand Long Template PCR System Kit (Roche Diagnostics) with 300 nmol/L sense and anti-sense primers, and 50 ng cDNA or genomic DNA. .. The amplified Gfap UTR amplicon was digested with EcoRI and XhoI before ligating the insert into pEZX-MT01 plasmid containing the luciferase reporter gene (GeneCopoeia, Rockville, MD).

Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
Article Snippet: .. Yeast DNA was isolated from strain BY4743 as described (Sambrook and Russell, ); a 5.8 kb DNA fragment encoding the RPO41 gene (Masters et al. , ) was amplified from yeast chromosomal DNA using an Expand Long Template PCR System kit (Roche Applied Science), subcloned into pT7Blue vector, using a Perfectly Blunt cloning kit (EMD Bioscience/Novagen), and recloned into pRS316 and pRS315 yeast shuffling vectors (centromeric plasmids that exist as a single copy in the cell and thus ensure the lack of overexpression; Sikorski and Hieter, ) to produce pRS316–Rpo41p and pRS315–Rpo41p, respectively. .. Competent cells were prepared and transfected with the shuffling constructs as described in BD Yeastmaker Yeast Transformation System 2 User Manual (BD Biosciences).

Article Title: Genomic characterization of large rearrangements of the LDLR gene in Czech patients with familial hypercholesterolemia
Article Snippet: .. Long-range PCR were performed using Expand Long Template PCR System Kit (Roche) and PCR amplifying fragments around breakpoints using AmpliTaq Gold polymerase (Applied Biosystems). .. PCR products were purified and sequenced on ABI PRISM 310 DNA-sequencer (Applied Biosystems).

Article Title: Molecular and Clinical Investigation of Iranian Patients with Friedreich Ataxia
Article Snippet: .. Long-range PCR was set up according to the protocol of Expand Long Template PCR System kit (Roche, Mannheim, Germany). .. The primers 5200Eco (5’-GGG CTG GCA GAT TCC TCC AG-3’) and 5200Not (5’-TAA GTA TCC GCG CCG GGA AC-3’) generated a 1.5-kb normal fragment.

Over Expression:

Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
Article Snippet: .. Yeast DNA was isolated from strain BY4743 as described (Sambrook and Russell, ); a 5.8 kb DNA fragment encoding the RPO41 gene (Masters et al. , ) was amplified from yeast chromosomal DNA using an Expand Long Template PCR System kit (Roche Applied Science), subcloned into pT7Blue vector, using a Perfectly Blunt cloning kit (EMD Bioscience/Novagen), and recloned into pRS316 and pRS315 yeast shuffling vectors (centromeric plasmids that exist as a single copy in the cell and thus ensure the lack of overexpression; Sikorski and Hieter, ) to produce pRS316–Rpo41p and pRS315–Rpo41p, respectively. .. Competent cells were prepared and transfected with the shuffling constructs as described in BD Yeastmaker Yeast Transformation System 2 User Manual (BD Biosciences).

Plasmid Preparation:

Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
Article Snippet: .. Yeast DNA was isolated from strain BY4743 as described (Sambrook and Russell, ); a 5.8 kb DNA fragment encoding the RPO41 gene (Masters et al. , ) was amplified from yeast chromosomal DNA using an Expand Long Template PCR System kit (Roche Applied Science), subcloned into pT7Blue vector, using a Perfectly Blunt cloning kit (EMD Bioscience/Novagen), and recloned into pRS316 and pRS315 yeast shuffling vectors (centromeric plasmids that exist as a single copy in the cell and thus ensure the lack of overexpression; Sikorski and Hieter, ) to produce pRS316–Rpo41p and pRS315–Rpo41p, respectively. .. Competent cells were prepared and transfected with the shuffling constructs as described in BD Yeastmaker Yeast Transformation System 2 User Manual (BD Biosciences).

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    Roche expand long template pcr kit
    Characterization and suitability of the <t>HEMn</t> cell system: The OCA2 gene is differentially expressed in HEMn-LP and HEMn-DP cells. ( A ) overview of the OCA2 - HERC2 locus ( top panel). ( Middle panel) The region covered on BAC RP11-1365A12. Vertebrate conservation (green); the position of rs12913832 (red). ( Lower panel) A schematic overview of the region investigated in this study. Restriction enzyme digestion sites are indicated. ( B ) Sequence analysis of the region around HERC2 rs12913832 in HEMn-LP ( left ) and HEMn-DP ( right ). The genotypes of rs12913832 were determined by direct sequencing of <t>PCR</t> fragments containing rs12913832. ( C ) RT-qPCR analysis of OCA2 primary transcripts in MCF7 and HEMn cells demonstrates differential OCA2 expression between HEMn-LP and HEMn-DP cells. Each gene expression analysis is carried out in triplicate and normalized to an endogenous reference gene ( ACTB ). ( D ) ChIP-qPCR of RNA Pol II binding at the OCA2 promoter in MCF7, HEMn-LP, and HEMn-DP cells. Enrichment is calculated relative to necdin ( NDN ), and values are normalized to input measurements. All ChIP analyses are performed in triplicate. Data are represented as mean ± SEM; (*) p
    Expand Long Template Pcr Kit, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long template pcr kit/product/Roche
    Average 91 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    expand long template pcr kit - by Bioz Stars, 2020-08
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    Characterization and suitability of the HEMn cell system: The OCA2 gene is differentially expressed in HEMn-LP and HEMn-DP cells. ( A ) overview of the OCA2 - HERC2 locus ( top panel). ( Middle panel) The region covered on BAC RP11-1365A12. Vertebrate conservation (green); the position of rs12913832 (red). ( Lower panel) A schematic overview of the region investigated in this study. Restriction enzyme digestion sites are indicated. ( B ) Sequence analysis of the region around HERC2 rs12913832 in HEMn-LP ( left ) and HEMn-DP ( right ). The genotypes of rs12913832 were determined by direct sequencing of PCR fragments containing rs12913832. ( C ) RT-qPCR analysis of OCA2 primary transcripts in MCF7 and HEMn cells demonstrates differential OCA2 expression between HEMn-LP and HEMn-DP cells. Each gene expression analysis is carried out in triplicate and normalized to an endogenous reference gene ( ACTB ). ( D ) ChIP-qPCR of RNA Pol II binding at the OCA2 promoter in MCF7, HEMn-LP, and HEMn-DP cells. Enrichment is calculated relative to necdin ( NDN ), and values are normalized to input measurements. All ChIP analyses are performed in triplicate. Data are represented as mean ± SEM; (*) p

    Journal: Genome Research

    Article Title: HERC2 rs12913832 modulates human pigmentation by attenuating chromatin-loop formation between a long-range enhancer and the OCA2 promoter

    doi: 10.1101/gr.128652.111

    Figure Lengend Snippet: Characterization and suitability of the HEMn cell system: The OCA2 gene is differentially expressed in HEMn-LP and HEMn-DP cells. ( A ) overview of the OCA2 - HERC2 locus ( top panel). ( Middle panel) The region covered on BAC RP11-1365A12. Vertebrate conservation (green); the position of rs12913832 (red). ( Lower panel) A schematic overview of the region investigated in this study. Restriction enzyme digestion sites are indicated. ( B ) Sequence analysis of the region around HERC2 rs12913832 in HEMn-LP ( left ) and HEMn-DP ( right ). The genotypes of rs12913832 were determined by direct sequencing of PCR fragments containing rs12913832. ( C ) RT-qPCR analysis of OCA2 primary transcripts in MCF7 and HEMn cells demonstrates differential OCA2 expression between HEMn-LP and HEMn-DP cells. Each gene expression analysis is carried out in triplicate and normalized to an endogenous reference gene ( ACTB ). ( D ) ChIP-qPCR of RNA Pol II binding at the OCA2 promoter in MCF7, HEMn-LP, and HEMn-DP cells. Enrichment is calculated relative to necdin ( NDN ), and values are normalized to input measurements. All ChIP analyses are performed in triplicate. Data are represented as mean ± SEM; (*) p

    Article Snippet: A 1450-bp fragment surrounding rs12913832 was PCR-amplified from genomic DNA obtained from HEMn-LP (C-allele) or HEMn-DP (T-allele) using the Expand Long Template PCR Kit (Roche).

    Techniques: BAC Assay, Sequencing, Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Binding Assay

    FAIRE analysis of pigmentation-associated SNPs other than HERC2 rs12913832 present within the 3′ HERC2 /5′ OCA2 region does not reveal additional regulatory elements. ( Left ) of the investigated 3′ HERC2/ 5′ OCA2 region. Pigmentation-associated SNPs (red); linked SNPs ( r 2 > 0.8) (black). The approximate location of the analyzed PCR amplicons is indicated. ( Right side) The genotype of each SNP for HEMn-LP and HEMn-DP. The enrichments displayed are relative to NDN . Data are represented as mean ± SEM.

    Journal: Genome Research

    Article Title: HERC2 rs12913832 modulates human pigmentation by attenuating chromatin-loop formation between a long-range enhancer and the OCA2 promoter

    doi: 10.1101/gr.128652.111

    Figure Lengend Snippet: FAIRE analysis of pigmentation-associated SNPs other than HERC2 rs12913832 present within the 3′ HERC2 /5′ OCA2 region does not reveal additional regulatory elements. ( Left ) of the investigated 3′ HERC2/ 5′ OCA2 region. Pigmentation-associated SNPs (red); linked SNPs ( r 2 > 0.8) (black). The approximate location of the analyzed PCR amplicons is indicated. ( Right side) The genotype of each SNP for HEMn-LP and HEMn-DP. The enrichments displayed are relative to NDN . Data are represented as mean ± SEM.

    Article Snippet: A 1450-bp fragment surrounding rs12913832 was PCR-amplified from genomic DNA obtained from HEMn-LP (C-allele) or HEMn-DP (T-allele) using the Expand Long Template PCR Kit (Roche).

    Techniques: Polymerase Chain Reaction

    Low- and high-GC PCR and TaqI RFLP assays. (A) Low-GC PCR amplicons and TaqI RFLP patterns. The top panel shows low-GC PCR amplicons from 20 poxvirus DNA samples. The bottom panel shows corresponding TaqI restriction endonuclease RFLP patterns. (B) High-GC

    Journal: Journal of Clinical Microbiology

    Article Title: GC Content-Based Pan-Pox Universal PCR Assays for Poxvirus Detection ▿

    doi: 10.1128/JCM.01697-09

    Figure Lengend Snippet: Low- and high-GC PCR and TaqI RFLP assays. (A) Low-GC PCR amplicons and TaqI RFLP patterns. The top panel shows low-GC PCR amplicons from 20 poxvirus DNA samples. The bottom panel shows corresponding TaqI restriction endonuclease RFLP patterns. (B) High-GC

    Article Snippet: PCR mixtures contained ∼10 to 100 ng of viral DNA and a 20 μM primer pair in 50 μl of a solution of 50 mM Tris-HCl buffer (pH 9.2); 16 mM (NH4 )2 SO4 ; 2.25 mM MgCl2 ; 2% (vol/vol) dimethyl sulfoxide; 0.1% (vol/vol) detergent Tween 20; 350 μM (each) dATP, dCTP, dGTP, and dTTP; and 2 units of the DNA polymerases Taq and Pwo provided in the Expand Long Template PCR Kit (Roche Molecular Biologicals, Indianapolis, IN).

    Techniques: Polymerase Chain Reaction

    HRV-5 is an ERV of rabbits. Genomic DNA from several mammalian and avian species was tested for the presence of HRV-5 sequences. (A) Southern blots probed with the MA domain of HRV-5 gag. Kbp, kilobase pairs. (B) PCR products obtained from the same species with primers derived from HRV-5 PR/RT, IN, and LTR and with degenerate primers for a gammaretrovirus pol sequence (γ). Following PCR, the HRV-5 products were transferred to a nylon membrane and were probed with an internal fragment specific for each product. Lanes: w, water; 1, fat-tailed dunnart; 2, quail; 3, Syrian hamster; 4, Chinese hamster; 5, rat; 6, mouse; 7, bat; 8, marmoset; 9, macaque; 10 African green monkey; 11, gibbon; 12, chimpanzee; 13, human (donor blood); 14, human (HeLa); 15, horse; 16, mink; 17, cat; 18, dog; 19, cow; 20, pig; 21, rabbit (SIRC); 22, rabbit (EREp), 23, rabbit spleen; 24, European hare; 25, black-tailed jackrabbit; and 26, Afghan pika. HRV-5 sequences were detected in rabbit DNA from three sources. (C) Southern blot of several species of Lagomorpha probed with the MA domain of HRV-5 gag .

    Journal: Journal of Virology

    Article Title: Novel Endogenous Retrovirus in Rabbits Previously Reported as Human Retrovirus 5

    doi: 10.1128/JVI.76.14.7094-7102.2002

    Figure Lengend Snippet: HRV-5 is an ERV of rabbits. Genomic DNA from several mammalian and avian species was tested for the presence of HRV-5 sequences. (A) Southern blots probed with the MA domain of HRV-5 gag. Kbp, kilobase pairs. (B) PCR products obtained from the same species with primers derived from HRV-5 PR/RT, IN, and LTR and with degenerate primers for a gammaretrovirus pol sequence (γ). Following PCR, the HRV-5 products were transferred to a nylon membrane and were probed with an internal fragment specific for each product. Lanes: w, water; 1, fat-tailed dunnart; 2, quail; 3, Syrian hamster; 4, Chinese hamster; 5, rat; 6, mouse; 7, bat; 8, marmoset; 9, macaque; 10 African green monkey; 11, gibbon; 12, chimpanzee; 13, human (donor blood); 14, human (HeLa); 15, horse; 16, mink; 17, cat; 18, dog; 19, cow; 20, pig; 21, rabbit (SIRC); 22, rabbit (EREp), 23, rabbit spleen; 24, European hare; 25, black-tailed jackrabbit; and 26, Afghan pika. HRV-5 sequences were detected in rabbit DNA from three sources. (C) Southern blot of several species of Lagomorpha probed with the MA domain of HRV-5 gag .

    Article Snippet: HRV-5 proviral clones were amplified from 200 ng of SIRC DNA using the Expand Long Template PCR kit (Roche).

    Techniques: Polymerase Chain Reaction, Derivative Assay, Sequencing, Southern Blot

    HRV-5 RNA expression in rabbit tissues. The expression of HRV-5 RNA was analyzed in several rabbit tissues by RT-PCR with primer sets from gag (NC) and pol (IN). Lanes: 1, spleen; 2, kidney; 3, liver; 4, ovary; 5, stomach; 6, colon; 7, pancreas; 8, heart; 9, skeletal muscle; 10, lung; 11 skin; 12, brain; 13, placenta; 14, mammary gland; 15, lymph node; 16, SIRC (rabbit corneal epithelial cell line), 17, human L363 cell RNA; w, PCR water control; +, positive control (rabbit DNA); 18, water control from the DNase treatment step; and 19, water control from the primer-annealing step. Experiments were performed with or without RT in the cDNA synthesis reaction to control for contamination with cellular DNA. PCR primers for rabbit GAPDH were used as controls for RNA quality.

    Journal: Journal of Virology

    Article Title: Novel Endogenous Retrovirus in Rabbits Previously Reported as Human Retrovirus 5

    doi: 10.1128/JVI.76.14.7094-7102.2002

    Figure Lengend Snippet: HRV-5 RNA expression in rabbit tissues. The expression of HRV-5 RNA was analyzed in several rabbit tissues by RT-PCR with primer sets from gag (NC) and pol (IN). Lanes: 1, spleen; 2, kidney; 3, liver; 4, ovary; 5, stomach; 6, colon; 7, pancreas; 8, heart; 9, skeletal muscle; 10, lung; 11 skin; 12, brain; 13, placenta; 14, mammary gland; 15, lymph node; 16, SIRC (rabbit corneal epithelial cell line), 17, human L363 cell RNA; w, PCR water control; +, positive control (rabbit DNA); 18, water control from the DNase treatment step; and 19, water control from the primer-annealing step. Experiments were performed with or without RT in the cDNA synthesis reaction to control for contamination with cellular DNA. PCR primers for rabbit GAPDH were used as controls for RNA quality.

    Article Snippet: HRV-5 proviral clones were amplified from 200 ng of SIRC DNA using the Expand Long Template PCR kit (Roche).

    Techniques: RNA Expression, Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Positive Control

    Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Journal: BioMed Research International

    Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy

    doi: 10.1155/2014/256245

    Figure Lengend Snippet: Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Article Snippet: PCR Long-Range On absence of amplification of POLH exon 10, long PCR was performed using the Expand Long Template PCR System Kit (Expand Long Range dNTPack 700 units/μ L Roche).

    Techniques: Polymerase Chain Reaction, Marker