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Roche expand long range dntpack
Expand Long Range Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand long range dntpack/product/Roche
Average 90 stars, based on 85 article reviews
Price from $9.99 to $1999.99
expand long range dntpack - by Bioz Stars, 2020-07
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI
Article Snippet: .. For the PCR amplicons with the Expand Long Range dNTPack, the heat-inactivation procedure of the restriction enzyme also permitted end-filling of the restriction enzyme-digested fragments in the presence of the activated heat-resistant DNA polymerase and dNTPs. .. Blunt-ended DNA was purified with the AMPure XP bead kit (Life Technologies, CA, USA) and was used for the adenine-tailing reaction of the NEB kit.

Article Title: Genomic Flexibility of Human Endogenous Retrovirus Type K
Article Snippet: .. These nucleic acids then were analyzed by long-range PCR using the Expand long-range dNTPack (Roche Applied Science) and the following primer sets: 1, envFor (5′-AAGTTCTACAATGAACCCATCGG-3′) and rRev (5′-GGGAACCAGCGTTCAGCATA-3′); 2, envFor (5′-AAGTTCTACAATGAACCCATCGG-3′) and u5Rev (5′-CACCTGTGGGTGTTTCTCGT-3′); and 3, u3For (5′-TGGGCAATGGAATGTCTCGG-3′) and envRev (5′-TCCGATGGGTTCATTGTAGAACT-3′). .. The same primer scheme also was used with the control full-length HERV-KCON plasmid as the template for amplification.

Article Title: Novel deletions affecting the MEG3-DMR provide further evidence for a hierarchical regulation of imprinting in 14q32
Article Snippet: .. To determine the exact size of the deletions and to identify the breakpoints on the DNA sequence level a long-range PCR was carried out using the Expand long-range dNTPack (Roche, Mannheim, Germany). .. Primers were generated spanning the sites of MLPA probes which showed a normal dosage in the siblings (patients 2 and 3) or spanning SNP or copy number probes of the array in patient 1 (for primer sequences see ).

Article Title: The Genetic Diversity of the Nguni Breed of African Cattle (Bos spp.): Complete Mitochondrial Genomes of Haplogroup T1
Article Snippet: .. Long range PCR was undertaken using Roche’s Expand Long Range dNTPack and the following reagent concentrations: 1x Long Range Buffer, 0.4 µM of each primer, 0.4 mM of each dNTP, 3% (v/v) DMSO (dimethyl sulfoxide) and 2 units of enzyme. ..

Article Title: SAD-3, a Putative Helicase Required for Meiotic Silencing by Unpaired DNA, Interacts with Other Components of the Silencing Machinery
Article Snippet: .. PCR-based confirmation of genotypes was performed with the Expand Long Range dNTPack (Roche Applied Science, Indianapolis, IN). .. Photography and microscopy A Canon Power Shot S3 IS digital camera was used to take photographs.

Article Title: The Nuclear Cap-Binding Complex Mediates Meiotic Silencing by Unpaired DNA
Article Snippet: .. PCR-based confirmation of genotypes was performed with the Promega GoTaq Green Master Mix or the Roche Expand Long Range dNTPack. .. Bimolecular fluorescence complementation (BiFC) BiFC is an in vivo protein–protein interaction assay, and it relies on the reconstitution of the yellow fluorescent protein (YFP) when its nonfluorescing halves are brought together by two interacting proteins ( ; ).

Article Title: QIP, a Protein That Converts Duplex siRNA Into Single Strands, Is Required for Meiotic Silencing by Unpaired DNA
Article Snippet: .. DNA amplification by polymerase chain reaction (PCR) was conducted in a PTC-100 Peltier Thermal Cycler (MJ Research, Waltham, MA), using either the AccuPrime Pfx system (Invitrogen, Carlsbad, CA) or the Expand Long Range dNTPack (Roche Applied Science, Indianapolis). .. PCR products, when necessary, were cloned into the pCRII-TOPO vector (Invitrogen).

Sequencing:

Article Title: Novel deletions affecting the MEG3-DMR provide further evidence for a hierarchical regulation of imprinting in 14q32
Article Snippet: .. To determine the exact size of the deletions and to identify the breakpoints on the DNA sequence level a long-range PCR was carried out using the Expand long-range dNTPack (Roche, Mannheim, Germany). .. Primers were generated spanning the sites of MLPA probes which showed a normal dosage in the siblings (patients 2 and 3) or spanning SNP or copy number probes of the array in patient 1 (for primer sequences see ).

Amplification:

Article Title: QIP, a Protein That Converts Duplex siRNA Into Single Strands, Is Required for Meiotic Silencing by Unpaired DNA
Article Snippet: .. DNA amplification by polymerase chain reaction (PCR) was conducted in a PTC-100 Peltier Thermal Cycler (MJ Research, Waltham, MA), using either the AccuPrime Pfx system (Invitrogen, Carlsbad, CA) or the Expand Long Range dNTPack (Roche Applied Science, Indianapolis). .. PCR products, when necessary, were cloned into the pCRII-TOPO vector (Invitrogen).

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  • 88
    Roche expand long template pcr system kit
    Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
    Expand Long Template Pcr System Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long template pcr system kit/product/Roche
    Average 88 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    expand long template pcr system kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    Roche two step nested long range pcr protocol
    Alpha-synuclein overexpression results in oxidative DNA damage, alterations in respiratory chain complex I and mitochondrial DNA deletions in transgenic mice brains. (A) Immuno-histochemical detection of 8-OHdG suggesting increased oxidative DNA damage in α-Syn tg mice brains. (B) 8-OHdG levels were quantified on whole brain in α-Syn tg and non-transgenic control mice by ELISA. Scale bar represents 25 µm. (C) Long extension <t>PCR</t> showing large-scale <t>mtDNA</t> deletions in α-Syn tg mice. (D) Quantitative determination of mtDNA deletions by real-time PCR showing increased mtDNA deletion levels in the brain of α-Syn tg mice compared to control animals. (E) Immunostaining for α-Syn (green signal) and NeuN (red signal; arrow marks the identical neuron in double labeling) used for Laser Capture Microdissection (LCM). Scale bar represents 250 µm in the upper panel and 25 µm in the close-up lower panel. (F) Quantification of mtDNA deletion levels by real-time PCR on individual neurons with either intense or negative α-Syn immunoreactivity after LCM. (G) Western blot analysis of mitochondrial OXPHOS in brain homogenates from α-Syn tg and wt mice. Lane 1 shows mitochondrial proteins standard marker. (H) Densitometric analysis of the levels of Complexes I, II, III and IV. *p
    Two Step Nested Long Range Pcr Protocol, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two step nested long range pcr protocol/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    two step nested long range pcr protocol - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    89
    Roche expand long range dntpack kit
    Alpha-synuclein overexpression results in oxidative DNA damage, alterations in respiratory chain complex I and mitochondrial DNA deletions in transgenic mice brains. (A) Immuno-histochemical detection of 8-OHdG suggesting increased oxidative DNA damage in α-Syn tg mice brains. (B) 8-OHdG levels were quantified on whole brain in α-Syn tg and non-transgenic control mice by ELISA. Scale bar represents 25 µm. (C) Long extension <t>PCR</t> showing large-scale <t>mtDNA</t> deletions in α-Syn tg mice. (D) Quantitative determination of mtDNA deletions by real-time PCR showing increased mtDNA deletion levels in the brain of α-Syn tg mice compared to control animals. (E) Immunostaining for α-Syn (green signal) and NeuN (red signal; arrow marks the identical neuron in double labeling) used for Laser Capture Microdissection (LCM). Scale bar represents 250 µm in the upper panel and 25 µm in the close-up lower panel. (F) Quantification of mtDNA deletion levels by real-time PCR on individual neurons with either intense or negative α-Syn immunoreactivity after LCM. (G) Western blot analysis of mitochondrial OXPHOS in brain homogenates from α-Syn tg and wt mice. Lane 1 shows mitochondrial proteins standard marker. (H) Densitometric analysis of the levels of Complexes I, II, III and IV. *p
    Expand Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack kit/product/Roche
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack kit - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    Image Search Results


    Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Journal: BioMed Research International

    Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy

    doi: 10.1155/2014/256245

    Figure Lengend Snippet: Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Article Snippet: PCR Long-Range On absence of amplification of POLH exon 10, long PCR was performed using the Expand Long Template PCR System Kit (Expand Long Range dNTPack 700 units/μ L Roche).

    Techniques: Polymerase Chain Reaction, Marker

    Alpha-synuclein overexpression results in oxidative DNA damage, alterations in respiratory chain complex I and mitochondrial DNA deletions in transgenic mice brains. (A) Immuno-histochemical detection of 8-OHdG suggesting increased oxidative DNA damage in α-Syn tg mice brains. (B) 8-OHdG levels were quantified on whole brain in α-Syn tg and non-transgenic control mice by ELISA. Scale bar represents 25 µm. (C) Long extension PCR showing large-scale mtDNA deletions in α-Syn tg mice. (D) Quantitative determination of mtDNA deletions by real-time PCR showing increased mtDNA deletion levels in the brain of α-Syn tg mice compared to control animals. (E) Immunostaining for α-Syn (green signal) and NeuN (red signal; arrow marks the identical neuron in double labeling) used for Laser Capture Microdissection (LCM). Scale bar represents 250 µm in the upper panel and 25 µm in the close-up lower panel. (F) Quantification of mtDNA deletion levels by real-time PCR on individual neurons with either intense or negative α-Syn immunoreactivity after LCM. (G) Western blot analysis of mitochondrial OXPHOS in brain homogenates from α-Syn tg and wt mice. Lane 1 shows mitochondrial proteins standard marker. (H) Densitometric analysis of the levels of Complexes I, II, III and IV. *p

    Journal: PLoS ONE

    Article Title: TOM40 Mediates Mitochondrial Dysfunction Induced by ?-Synuclein Accumulation in Parkinson's Disease

    doi: 10.1371/journal.pone.0062277

    Figure Lengend Snippet: Alpha-synuclein overexpression results in oxidative DNA damage, alterations in respiratory chain complex I and mitochondrial DNA deletions in transgenic mice brains. (A) Immuno-histochemical detection of 8-OHdG suggesting increased oxidative DNA damage in α-Syn tg mice brains. (B) 8-OHdG levels were quantified on whole brain in α-Syn tg and non-transgenic control mice by ELISA. Scale bar represents 25 µm. (C) Long extension PCR showing large-scale mtDNA deletions in α-Syn tg mice. (D) Quantitative determination of mtDNA deletions by real-time PCR showing increased mtDNA deletion levels in the brain of α-Syn tg mice compared to control animals. (E) Immunostaining for α-Syn (green signal) and NeuN (red signal; arrow marks the identical neuron in double labeling) used for Laser Capture Microdissection (LCM). Scale bar represents 250 µm in the upper panel and 25 µm in the close-up lower panel. (F) Quantification of mtDNA deletion levels by real-time PCR on individual neurons with either intense or negative α-Syn immunoreactivity after LCM. (G) Western blot analysis of mitochondrial OXPHOS in brain homogenates from α-Syn tg and wt mice. Lane 1 shows mitochondrial proteins standard marker. (H) Densitometric analysis of the levels of Complexes I, II, III and IV. *p

    Article Snippet: Low-abundance mtDNA deletions were detected by a two-step nested long-range PCR protocol (Expand Long Range dNTPack®, Roche, Mannheim, Germany) to amplify a final ∼10 kb fragment of the deletion-prone “major arc” of the mitochondrial genome as we previously reported , .

    Techniques: Over Expression, Transgenic Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Immunostaining, Labeling, Laser Capture Microdissection, Western Blot, Marker