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Roche expand long range dntpack kit
Expand Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand long range dntpack kit/product/Roche
Average 89 stars, based on 20 article reviews
Price from $9.99 to $1999.99
expand long range dntpack kit - by Bioz Stars, 2020-08
89/100 stars

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Multiplex Assay:

Article Title: Mitochondrial Genome Sequences Effectively Reveal the Phylogeny of Hylobates Gibbons
Article Snippet: .. Two-step multiplex long-range PCR We amplified the entire mtgenomes in four large overlapping fragments (∼3.8–5 kb each) by two-step multiplex long-range PCR using the Expand Long Range dNTPack kit (Roche). .. In the first multiplex step, the four primer pairs were divided into two sets, A and B ( ).

Amplification:

Article Title: Next generation sequencing and comparative analyses of Xenopus mitogenomes
Article Snippet: .. Long-PCR amplification of two mitochondrial genome regions The complete mitochondrial genome of each Xenopus species was amplified by long-PCR as two amplicons (1: ~7,961bp, containing the rrnL and cox1 genes and 2: ~9,649bp) using the Expand Long Range dNTPack kit (Roche). .. Each (50 μL) PCR contained 10 ng total DNA; 2 x buffer, 2.5 mM MgCl2, 0.5 μM each dNTP, 0.3 μM each of primers Long F1 and R2 (Amplicon 1) or Long F2 and R1 (Amplicon 2; Table and Figure ), 1.4% (v/v) DMSO and 0.7 μl of enzyme mix and was run on a GeneAmp® PCR system 9700 at 92°C for 2 min; 10 cycles at 92°C for 10 s, 55°C for 15 s, 68°C for 10 min; 20 cycles at 92°C for 10 s, 55°C for 15 s, 68°C for 10 min + 10 s per cycle; followed by 68°C for 7 min. Amplicons were resolved on 1% (w/v) agarose gels at 100V for 1 h, purified using the QIAquick® PCR purification Kit (QIAGEN, Hilden, Germany) and quantified using the Quant-iT™ PicoGreen® dsDNA assay Kit (Invitrogen) and a Spectramax microplate reader; Molecular Devices Ltd, Wokingham, UK).

Article Title: Mitochondrial Genome Sequences Effectively Reveal the Phylogeny of Hylobates Gibbons
Article Snippet: .. Two-step multiplex long-range PCR We amplified the entire mtgenomes in four large overlapping fragments (∼3.8–5 kb each) by two-step multiplex long-range PCR using the Expand Long Range dNTPack kit (Roche). .. In the first multiplex step, the four primer pairs were divided into two sets, A and B ( ).

Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI
Article Snippet: .. For a further exemplification of the effects on distribution of fragments generated with a methylation-dependent restriction enzyme, the Bt KIT1-10 and Bt KIT27-37 sequences were amplified from Bt gDNA, using the Roche Expand Long Range dNTPack kit in the presence of 7.5, 15 and 60 μM 5-methyl-dCTP (Additional file ). ..

Article Title: Expanding the Host Range of Hepatitis C Virus through Viral Adaptation
Article Snippet: .. For amplicon library preparation, six contigs which covered the signal peptide genes of E1, E2, and p7 (amino acids 131 to 813, corresponding to the polyprotein sequence of the reference strain Jc1) were generated with the Expand Long Range dNTPack kit (Roche, Basel, Switzerland) by nested PCR utilizing 12 primers tagged at the 5′ end. .. Amplicons were prepared individually and quantified by using the Quant-iT PicoGreen dsDNA (double-stranded DNA) assay kit (Carlsbad, CA).

Article Title: Targeted high-throughput sequencing of tagged nucleic acid samples
Article Snippet: .. The mtDNA genomes were amplified in two overlapping fragments using the Expand Long Range dNTPack kit (Roche) and equimolar mixtures of 5′ blocked and unmodified PCR primers in order to avoid sequence overrepresentation within the two amplicon overlaps (oligonucleotide sequences listed in Supplementary Table 2). .. Amplicons were purified using the PCR purification kit (Qiagen) and quantified using an ND-1000 spectrophotometer (Nanodrop Technologies).

Article Title: Prevalence and genetic variants of G6PD deficiency among two Malagasy populations living in Plasmodium vivax-endemic areas
Article Snippet: .. Amplification conditions were 95 °C for 2 min, followed by 30 cycles of 95 °C for 45 s, 59 °C for 45 s, and 72 °C for 45 s and a final extension at 72 °C for 5 min. For fragments B (exons 3–8) and C (exons 9–13), PCR amplification was performed using the Roche Expand Long Range dNTPack Kit (Indianapolis, IN) in a final reaction mixture of 50 μL; containing 3 μL of genomic DNA, 0.3 μM of each primer (primer set B) or 0.45 μM of each primer (primer set C), 500 μM of each dNTP, 2.5 mM of MgCl2 , 1.25% DMSO and 3.5 units of Expand Long Range enzyme mix. .. G6PD gene sequencing PCR products were sheared on a Covaris machine for an estimated size of 300 bp.

Methylation:

Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI
Article Snippet: .. For a further exemplification of the effects on distribution of fragments generated with a methylation-dependent restriction enzyme, the Bt KIT1-10 and Bt KIT27-37 sequences were amplified from Bt gDNA, using the Roche Expand Long Range dNTPack kit in the presence of 7.5, 15 and 60 μM 5-methyl-dCTP (Additional file ). ..

Mutagenesis:

Article Title: Identification of Human Astrovirus Genome-Linked Protein (VPg) Essential for Virus Infectivity
Article Snippet: .. Full-length mutant and wild-type HAstV cDNAs were obtained from the corresponding plasmids by PCR using the Expand Long Range dNTPack kit (Roche). .. The primers used in the PCR were 5′ end modified to contain the T7 promoter sequence and 3′ end modified to contain a 43-mer poly(T) tail.

Polymerase Chain Reaction:

Article Title: Mitochondrial Genome Sequences Effectively Reveal the Phylogeny of Hylobates Gibbons
Article Snippet: .. Two-step multiplex long-range PCR We amplified the entire mtgenomes in four large overlapping fragments (∼3.8–5 kb each) by two-step multiplex long-range PCR using the Expand Long Range dNTPack kit (Roche). .. In the first multiplex step, the four primer pairs were divided into two sets, A and B ( ).

Article Title: Generation of a tamoxifen inducible Tnnt2MerCreMer knock-in mouse model for cardiac studies
Article Snippet: .. Long range PCR was applied to screen the targeted ES cells using Expand Long Range dNTPack kit (Roche Cat # 04829042001) following the manufacturer’s instructions. .. PCR program consisted of an initial denaturation of 2 min at 92°C, 42 cycles of reactions (92 °C for 10 s; 60 °C for 15 s, 68 °C for 4 min), and a final elongation of 10 min at 68 °C.

Article Title: Targeted high-throughput sequencing of tagged nucleic acid samples
Article Snippet: .. The mtDNA genomes were amplified in two overlapping fragments using the Expand Long Range dNTPack kit (Roche) and equimolar mixtures of 5′ blocked and unmodified PCR primers in order to avoid sequence overrepresentation within the two amplicon overlaps (oligonucleotide sequences listed in Supplementary Table 2). .. Amplicons were purified using the PCR purification kit (Qiagen) and quantified using an ND-1000 spectrophotometer (Nanodrop Technologies).

Article Title: Prevalence and genetic variants of G6PD deficiency among two Malagasy populations living in Plasmodium vivax-endemic areas
Article Snippet: .. Amplification conditions were 95 °C for 2 min, followed by 30 cycles of 95 °C for 45 s, 59 °C for 45 s, and 72 °C for 45 s and a final extension at 72 °C for 5 min. For fragments B (exons 3–8) and C (exons 9–13), PCR amplification was performed using the Roche Expand Long Range dNTPack Kit (Indianapolis, IN) in a final reaction mixture of 50 μL; containing 3 μL of genomic DNA, 0.3 μM of each primer (primer set B) or 0.45 μM of each primer (primer set C), 500 μM of each dNTP, 2.5 mM of MgCl2 , 1.25% DMSO and 3.5 units of Expand Long Range enzyme mix. .. G6PD gene sequencing PCR products were sheared on a Covaris machine for an estimated size of 300 bp.

Article Title: Identification of Human Astrovirus Genome-Linked Protein (VPg) Essential for Virus Infectivity
Article Snippet: .. Full-length mutant and wild-type HAstV cDNAs were obtained from the corresponding plasmids by PCR using the Expand Long Range dNTPack kit (Roche). .. The primers used in the PCR were 5′ end modified to contain the T7 promoter sequence and 3′ end modified to contain a 43-mer poly(T) tail.

Generated:

Article Title: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI
Article Snippet: .. For a further exemplification of the effects on distribution of fragments generated with a methylation-dependent restriction enzyme, the Bt KIT1-10 and Bt KIT27-37 sequences were amplified from Bt gDNA, using the Roche Expand Long Range dNTPack kit in the presence of 7.5, 15 and 60 μM 5-methyl-dCTP (Additional file ). ..

Article Title: Expanding the Host Range of Hepatitis C Virus through Viral Adaptation
Article Snippet: .. For amplicon library preparation, six contigs which covered the signal peptide genes of E1, E2, and p7 (amino acids 131 to 813, corresponding to the polyprotein sequence of the reference strain Jc1) were generated with the Expand Long Range dNTPack kit (Roche, Basel, Switzerland) by nested PCR utilizing 12 primers tagged at the 5′ end. .. Amplicons were prepared individually and quantified by using the Quant-iT PicoGreen dsDNA (double-stranded DNA) assay kit (Carlsbad, CA).

Sequencing:

Article Title: Expanding the Host Range of Hepatitis C Virus through Viral Adaptation
Article Snippet: .. For amplicon library preparation, six contigs which covered the signal peptide genes of E1, E2, and p7 (amino acids 131 to 813, corresponding to the polyprotein sequence of the reference strain Jc1) were generated with the Expand Long Range dNTPack kit (Roche, Basel, Switzerland) by nested PCR utilizing 12 primers tagged at the 5′ end. .. Amplicons were prepared individually and quantified by using the Quant-iT PicoGreen dsDNA (double-stranded DNA) assay kit (Carlsbad, CA).

Article Title: Targeted high-throughput sequencing of tagged nucleic acid samples
Article Snippet: .. The mtDNA genomes were amplified in two overlapping fragments using the Expand Long Range dNTPack kit (Roche) and equimolar mixtures of 5′ blocked and unmodified PCR primers in order to avoid sequence overrepresentation within the two amplicon overlaps (oligonucleotide sequences listed in Supplementary Table 2). .. Amplicons were purified using the PCR purification kit (Qiagen) and quantified using an ND-1000 spectrophotometer (Nanodrop Technologies).

Nested PCR:

Article Title: Expanding the Host Range of Hepatitis C Virus through Viral Adaptation
Article Snippet: .. For amplicon library preparation, six contigs which covered the signal peptide genes of E1, E2, and p7 (amino acids 131 to 813, corresponding to the polyprotein sequence of the reference strain Jc1) were generated with the Expand Long Range dNTPack kit (Roche, Basel, Switzerland) by nested PCR utilizing 12 primers tagged at the 5′ end. .. Amplicons were prepared individually and quantified by using the Quant-iT PicoGreen dsDNA (double-stranded DNA) assay kit (Carlsbad, CA).

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  • 88
    Roche expand long template pcr system kit
    Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
    Expand Long Template Pcr System Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long template pcr system kit/product/Roche
    Average 88 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    expand long template pcr system kit - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    89
    Roche expand long range dntpack kit
    Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
    Expand Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack kit/product/Roche
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    85
    Roche long range dntpack kit
    Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
    Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range dntpack kit/product/Roche
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    long range dntpack kit - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Journal: BioMed Research International

    Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy

    doi: 10.1155/2014/256245

    Figure Lengend Snippet: Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Article Snippet: PCR Long-Range On absence of amplification of POLH exon 10, long PCR was performed using the Expand Long Template PCR System Kit (Expand Long Range dNTPack 700 units/μ L Roche).

    Techniques: Polymerase Chain Reaction, Marker