expand high fidelity  (takara)


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    takara expand high fidelity
    Expand High Fidelity, supplied by takara, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity/product/takara
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity - by Bioz Stars, 2021-09
    86/100 stars

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    Clone Assay:

    Article Title: Molecular and functional characterization of acid-sensing ion channel (ASIC) 1b.
    Article Snippet: .. ASIC1a was cloned from rat brain cDNA by PCR using Expand High Fidelity (Roche Molecular Biochemicals). ..

    Polymerase Chain Reaction:

    Article Title: Molecular and functional characterization of acid-sensing ion channel (ASIC) 1b.
    Article Snippet: .. ASIC1a was cloned from rat brain cDNA by PCR using Expand High Fidelity (Roche Molecular Biochemicals). ..

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    TaKaRa expand high fidelity pcr system
    Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
    Expand High Fidelity Pcr System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    96
    TaKaRa la taq
    Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
    La Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la taq/product/TaKaRa
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    la taq - by Bioz Stars, 2021-09
    96/100 stars
      Buy from Supplier

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    Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Journal: Journal of Zhejiang University. Science. B

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    doi: 10.1631/jzus.B1500008

    Figure Lengend Snippet: Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Article Snippet: The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China).

    Techniques: Polymerase Chain Reaction, Amplification, Isolation, Purification

    Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Journal: Journal of Zhejiang University. Science. B

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    doi: 10.1631/jzus.B1500008

    Figure Lengend Snippet: Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Article Snippet: The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China).

    Techniques: Recombinant, Plasmid Preparation, Positron Emission Tomography, Polymerase Chain Reaction, Amplification, Marker

    Gel electrophoresis of PCR products of α-gal gene. (a) (lane 1–3 represent PCR products of α-gal gene, M represents DNA Marker DL2000) and amino acid sequences analysis of AglB by BLASTn.

    Journal: PLoS ONE

    Article Title: Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties

    doi: 10.1371/journal.pone.0235687

    Figure Lengend Snippet: Gel electrophoresis of PCR products of α-gal gene. (a) (lane 1–3 represent PCR products of α-gal gene, M represents DNA Marker DL2000) and amino acid sequences analysis of AglB by BLASTn.

    Article Snippet: The Expand High Fidelity PCR System with PrimeSTAR® HS DNA Polymerase (Takara, Dalian, China) was used to clone gene aga1 (Accession number: CP020457) with oligonucleotides: 5’- CCG GAATTC ATGAA CCACGAACTAATCAC-3′ containing a EcoR I site (underlined) and 5′-CCG CTCGAG TTAATTCCGTACACTGTTTG-3′ containing a Xho I site (underlined).

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Marker