expand high fidelity pcr system  (TaKaRa)


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    Name:
    High Fidelity PCR EcoDry Premix
    Description:

    Catalog Number:
    639280
    Price:
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    Category:
    PCR
    Size:
    48 Rxns
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    Structured Review

    TaKaRa expand high fidelity pcr system
    Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    https://www.bioz.com/result/expand high fidelity pcr system/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2021-07
    86/100 stars

    Images

    1) Product Images from "Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *"

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    Journal: Journal of Zhejiang University. Science. B

    doi: 10.1631/jzus.B1500008

    Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
    Figure Legend Snippet: Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Techniques Used: Polymerase Chain Reaction, Amplification, Isolation, Purification

    Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker
    Figure Legend Snippet: Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Techniques Used: Recombinant, Plasmid Preparation, Positron Emission Tomography, Polymerase Chain Reaction, Amplification, Marker

    2) Product Images from "Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties"

    Article Title: Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0235687

    Gel electrophoresis of PCR products of α-gal gene. (a) (lane 1–3 represent PCR products of α-gal gene, M represents DNA Marker DL2000) and amino acid sequences analysis of AglB by BLASTn.
    Figure Legend Snippet: Gel electrophoresis of PCR products of α-gal gene. (a) (lane 1–3 represent PCR products of α-gal gene, M represents DNA Marker DL2000) and amino acid sequences analysis of AglB by BLASTn.

    Techniques Used: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Marker

    Related Articles

    Sequencing:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain Δ stsR /pDL278- stsR , which was further confirmed by PCR and sequencing. .. The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: Overexpression of SlOFP20 affects floral organ and pollen development
    Article Snippet: The Tomato Solanaceae Genomics Network (SGN) unigene accession numbers were as follows: SlOFP5 (Solyc02g072030), SlOFP14 (Solyc06g082460), SlOFP15 (Solyc07g055240), SlOFP17 (Solyc09g018200), and SlOFP20 (Solyc10g076180). .. Vector construction and tomato transformation For the overexpression of SlOFP20 in WT tomato, the full-length sequence was amplified by high-fidelity PCR (Prime START mix DNA polymerase, Takara) with the SlOFP20 -F and SlOFP20 -R primers (Supplementary Table ), which were tailed with Xba I and Sac I restriction sites, respectively, at their 5′ ends. .. A DNA-Tailing kit (Takara) was applied to tail the obtained PCR products, which were then linked with the pMD18-T vector (Takara).

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: cyclops encodes a nodal-related factor involved in midline signaling
    Article Snippet: PCR conditions were: 40 μCi 32 P-dCTP, 100 ng of genomic DNA, 50 ng of each primer; hot start, 94°, 1 min; 65°, 1 min; 72°, 45 s, 45 cycles. .. For sequencing of cyc tf219 DNA, high fidelity PCR (Klentaq, CLONTECH, or TaqPlus Precision, Stratagene) was used with primers based on the wild-type sequence ( ) to generate fragments spanning the ndr2 gene in mutant DNA. .. The wild-type construct in the CS2+ vector ( ) contained the entire cyc ORF ( ).

    Amplification:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain Δ stsR /pDL278- stsR , which was further confirmed by PCR and sequencing. .. The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: Overexpression of SlOFP20 affects floral organ and pollen development
    Article Snippet: The Tomato Solanaceae Genomics Network (SGN) unigene accession numbers were as follows: SlOFP5 (Solyc02g072030), SlOFP14 (Solyc06g082460), SlOFP15 (Solyc07g055240), SlOFP17 (Solyc09g018200), and SlOFP20 (Solyc10g076180). .. Vector construction and tomato transformation For the overexpression of SlOFP20 in WT tomato, the full-length sequence was amplified by high-fidelity PCR (Prime START mix DNA polymerase, Takara) with the SlOFP20 -F and SlOFP20 -R primers (Supplementary Table ), which were tailed with Xba I and Sac I restriction sites, respectively, at their 5′ ends. .. A DNA-Tailing kit (Takara) was applied to tail the obtained PCR products, which were then linked with the pMD18-T vector (Takara).

    Article Title: Differential gene expression between the vigorous and dwarf litchi cultivars based on RNA-Seq transcriptome analysis
    Article Snippet: Unigene expression levels were calculated using the 2ΔΔCT method [ ]. .. Isolation of LcGA2ox genes and functional analysis in tobacco The full length of LcGA2ox1-3 genes were amplified by PCR with primers ( ) through high fidelity PCR (Prime STARTM HS DNA polymerase, Takara). .. The PCR products were digested with Bam HI and Sac I respectively, and fused into the plant binary vector pBI121 digested by the same enzymes.

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome
    Article Snippet: .. A full-length (FL) human SALL1 clone was used for high-fidelity PCR amplification and subcloning into EYFP-N1 (Clontech) as truncated ( SALL1c.826C>T -YFP ) or FL ( SALL1 FL -YFP ) versions. .. Clones, corresponding to current annotations, were validated by Sanger sequencing (GenBank: ).

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Polymerase Chain Reaction:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain Δ stsR /pDL278- stsR , which was further confirmed by PCR and sequencing. .. The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: Overexpression of SlOFP20 affects floral organ and pollen development
    Article Snippet: The Tomato Solanaceae Genomics Network (SGN) unigene accession numbers were as follows: SlOFP5 (Solyc02g072030), SlOFP14 (Solyc06g082460), SlOFP15 (Solyc07g055240), SlOFP17 (Solyc09g018200), and SlOFP20 (Solyc10g076180). .. Vector construction and tomato transformation For the overexpression of SlOFP20 in WT tomato, the full-length sequence was amplified by high-fidelity PCR (Prime START mix DNA polymerase, Takara) with the SlOFP20 -F and SlOFP20 -R primers (Supplementary Table ), which were tailed with Xba I and Sac I restriction sites, respectively, at their 5′ ends. .. A DNA-Tailing kit (Takara) was applied to tail the obtained PCR products, which were then linked with the pMD18-T vector (Takara).

    Article Title: Differential gene expression between the vigorous and dwarf litchi cultivars based on RNA-Seq transcriptome analysis
    Article Snippet: Unigene expression levels were calculated using the 2ΔΔCT method [ ]. .. Isolation of LcGA2ox genes and functional analysis in tobacco The full length of LcGA2ox1-3 genes were amplified by PCR with primers ( ) through high fidelity PCR (Prime STARTM HS DNA polymerase, Takara). .. The PCR products were digested with Bam HI and Sac I respectively, and fused into the plant binary vector pBI121 digested by the same enzymes.

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: cyclops encodes a nodal-related factor involved in midline signaling
    Article Snippet: PCR conditions were: 40 μCi 32 P-dCTP, 100 ng of genomic DNA, 50 ng of each primer; hot start, 94°, 1 min; 65°, 1 min; 72°, 45 s, 45 cycles. .. For sequencing of cyc tf219 DNA, high fidelity PCR (Klentaq, CLONTECH, or TaqPlus Precision, Stratagene) was used with primers based on the wild-type sequence ( ) to generate fragments spanning the ndr2 gene in mutant DNA. .. The wild-type construct in the CS2+ vector ( ) contained the entire cyc ORF ( ).

    Article Title: Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome
    Article Snippet: .. A full-length (FL) human SALL1 clone was used for high-fidelity PCR amplification and subcloning into EYFP-N1 (Clontech) as truncated ( SALL1c.826C>T -YFP ) or FL ( SALL1 FL -YFP ) versions. .. Clones, corresponding to current annotations, were validated by Sanger sequencing (GenBank: ).

    Article Title: In Vivo Analysis of MEF2 Transcription Factors in Synapse Regulation and Neuronal Survival
    Article Snippet: .. Generation of MEF2A conditional mice Genomic regions of the Mef2a locus were isolated from 129SvEv genomic DNA by high-fidelity PCR (Takara LA taq PCR system) and cloned into a pGKneoF2L2DTA vector, which contains a neomycin resistance gene, flanked by FRT and loxP sites, and a diphtheria toxin gene cassette. .. A 1.7-kb genomic sequence (5′ arm) upstream of Mef2a exon 2 was cloned upstream of a 5′ loxP sequence in the pGKneoF2L2DTA vector.

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Plasmid Preparation:

    Article Title: Overexpression of SlOFP20 affects floral organ and pollen development
    Article Snippet: The Tomato Solanaceae Genomics Network (SGN) unigene accession numbers were as follows: SlOFP5 (Solyc02g072030), SlOFP14 (Solyc06g082460), SlOFP15 (Solyc07g055240), SlOFP17 (Solyc09g018200), and SlOFP20 (Solyc10g076180). .. Vector construction and tomato transformation For the overexpression of SlOFP20 in WT tomato, the full-length sequence was amplified by high-fidelity PCR (Prime START mix DNA polymerase, Takara) with the SlOFP20 -F and SlOFP20 -R primers (Supplementary Table ), which were tailed with Xba I and Sac I restriction sites, respectively, at their 5′ ends. .. A DNA-Tailing kit (Takara) was applied to tail the obtained PCR products, which were then linked with the pMD18-T vector (Takara).

    Article Title: In Vivo Analysis of MEF2 Transcription Factors in Synapse Regulation and Neuronal Survival
    Article Snippet: .. Generation of MEF2A conditional mice Genomic regions of the Mef2a locus were isolated from 129SvEv genomic DNA by high-fidelity PCR (Takara LA taq PCR system) and cloned into a pGKneoF2L2DTA vector, which contains a neomycin resistance gene, flanked by FRT and loxP sites, and a diphtheria toxin gene cassette. .. A 1.7-kb genomic sequence (5′ arm) upstream of Mef2a exon 2 was cloned upstream of a 5′ loxP sequence in the pGKneoF2L2DTA vector.

    Transformation Assay:

    Article Title: Overexpression of SlOFP20 affects floral organ and pollen development
    Article Snippet: The Tomato Solanaceae Genomics Network (SGN) unigene accession numbers were as follows: SlOFP5 (Solyc02g072030), SlOFP14 (Solyc06g082460), SlOFP15 (Solyc07g055240), SlOFP17 (Solyc09g018200), and SlOFP20 (Solyc10g076180). .. Vector construction and tomato transformation For the overexpression of SlOFP20 in WT tomato, the full-length sequence was amplified by high-fidelity PCR (Prime START mix DNA polymerase, Takara) with the SlOFP20 -F and SlOFP20 -R primers (Supplementary Table ), which were tailed with Xba I and Sac I restriction sites, respectively, at their 5′ ends. .. A DNA-Tailing kit (Takara) was applied to tail the obtained PCR products, which were then linked with the pMD18-T vector (Takara).

    Over Expression:

    Article Title: Overexpression of SlOFP20 affects floral organ and pollen development
    Article Snippet: The Tomato Solanaceae Genomics Network (SGN) unigene accession numbers were as follows: SlOFP5 (Solyc02g072030), SlOFP14 (Solyc06g082460), SlOFP15 (Solyc07g055240), SlOFP17 (Solyc09g018200), and SlOFP20 (Solyc10g076180). .. Vector construction and tomato transformation For the overexpression of SlOFP20 in WT tomato, the full-length sequence was amplified by high-fidelity PCR (Prime START mix DNA polymerase, Takara) with the SlOFP20 -F and SlOFP20 -R primers (Supplementary Table ), which were tailed with Xba I and Sac I restriction sites, respectively, at their 5′ ends. .. A DNA-Tailing kit (Takara) was applied to tail the obtained PCR products, which were then linked with the pMD18-T vector (Takara).

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Isolation:

    Article Title: Differential gene expression between the vigorous and dwarf litchi cultivars based on RNA-Seq transcriptome analysis
    Article Snippet: Unigene expression levels were calculated using the 2ΔΔCT method [ ]. .. Isolation of LcGA2ox genes and functional analysis in tobacco The full length of LcGA2ox1-3 genes were amplified by PCR with primers ( ) through high fidelity PCR (Prime STARTM HS DNA polymerase, Takara). .. The PCR products were digested with Bam HI and Sac I respectively, and fused into the plant binary vector pBI121 digested by the same enzymes.

    Article Title: In Vivo Analysis of MEF2 Transcription Factors in Synapse Regulation and Neuronal Survival
    Article Snippet: .. Generation of MEF2A conditional mice Genomic regions of the Mef2a locus were isolated from 129SvEv genomic DNA by high-fidelity PCR (Takara LA taq PCR system) and cloned into a pGKneoF2L2DTA vector, which contains a neomycin resistance gene, flanked by FRT and loxP sites, and a diphtheria toxin gene cassette. .. A 1.7-kb genomic sequence (5′ arm) upstream of Mef2a exon 2 was cloned upstream of a 5′ loxP sequence in the pGKneoF2L2DTA vector.

    Functional Assay:

    Article Title: Differential gene expression between the vigorous and dwarf litchi cultivars based on RNA-Seq transcriptome analysis
    Article Snippet: Unigene expression levels were calculated using the 2ΔΔCT method [ ]. .. Isolation of LcGA2ox genes and functional analysis in tobacco The full length of LcGA2ox1-3 genes were amplified by PCR with primers ( ) through high fidelity PCR (Prime STARTM HS DNA polymerase, Takara). .. The PCR products were digested with Bam HI and Sac I respectively, and fused into the plant binary vector pBI121 digested by the same enzymes.

    Clone Assay:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: In Vivo Analysis of MEF2 Transcription Factors in Synapse Regulation and Neuronal Survival
    Article Snippet: .. Generation of MEF2A conditional mice Genomic regions of the Mef2a locus were isolated from 129SvEv genomic DNA by high-fidelity PCR (Takara LA taq PCR system) and cloned into a pGKneoF2L2DTA vector, which contains a neomycin resistance gene, flanked by FRT and loxP sites, and a diphtheria toxin gene cassette. .. A 1.7-kb genomic sequence (5′ arm) upstream of Mef2a exon 2 was cloned upstream of a 5′ loxP sequence in the pGKneoF2L2DTA vector.

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Expressing:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Purification:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Recombinant:

    Article Title: A GntR Family Transcription Factor in Streptococcus mutans Regulates Biofilm Formation and Expression of Multiple Sugar Transporter Genes
    Article Snippet: This generated the complement strain ΔstsR /pDL278-stsR , which was further confirmed by PCR and sequencing. .. Cloning, Expression, and Purification of the Recombinant StsR Protein The S. mutans stsR gene coding sequence was amplified with primers SMU.1193F and SMU.1193R from S. mutans genomic DNA, using the high-fidelity PCR system (TakaRa). .. The recombinant vector pETstsR was produced by cloning the stsR coding sequence into the pET28a expression vector using the restriction sites listed in Supplementary Table .

    Article Title: The Mycobacterial LysR-Type Regulator OxyS Responds to Oxidative Stress and Negatively Regulates Expression of the Catalase-Peroxidase Gene
    Article Snippet: .. Cloning, expression and purification of recombinant proteins The M. tuberculosis oxyS gene was amplified from genomic DNA using the High Fidelity PCR system (TaKaRa) with appropriate primers and cloned into the pET28a over-expression vectors to produce recombinant vectors ( ). .. E. coli BL21 (DE3) cells transformed with the recombinant plasmid were grown at 37°C for 5 hours in 1 liter of LB medium containing 30 µg/ml−1 kanamycin, and at 20°C for 15 hours after 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside) was added.

    Mutagenesis:

    Article Title: cyclops encodes a nodal-related factor involved in midline signaling
    Article Snippet: PCR conditions were: 40 μCi 32 P-dCTP, 100 ng of genomic DNA, 50 ng of each primer; hot start, 94°, 1 min; 65°, 1 min; 72°, 45 s, 45 cycles. .. For sequencing of cyc tf219 DNA, high fidelity PCR (Klentaq, CLONTECH, or TaqPlus Precision, Stratagene) was used with primers based on the wild-type sequence ( ) to generate fragments spanning the ndr2 gene in mutant DNA. .. The wild-type construct in the CS2+ vector ( ) contained the entire cyc ORF ( ).

    Subcloning:

    Article Title: Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome
    Article Snippet: .. A full-length (FL) human SALL1 clone was used for high-fidelity PCR amplification and subcloning into EYFP-N1 (Clontech) as truncated ( SALL1c.826C>T -YFP ) or FL ( SALL1 FL -YFP ) versions. .. Clones, corresponding to current annotations, were validated by Sanger sequencing (GenBank: ).

    Mouse Assay:

    Article Title: In Vivo Analysis of MEF2 Transcription Factors in Synapse Regulation and Neuronal Survival
    Article Snippet: .. Generation of MEF2A conditional mice Genomic regions of the Mef2a locus were isolated from 129SvEv genomic DNA by high-fidelity PCR (Takara LA taq PCR system) and cloned into a pGKneoF2L2DTA vector, which contains a neomycin resistance gene, flanked by FRT and loxP sites, and a diphtheria toxin gene cassette. .. A 1.7-kb genomic sequence (5′ arm) upstream of Mef2a exon 2 was cloned upstream of a 5′ loxP sequence in the pGKneoF2L2DTA vector.

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    TaKaRa expand high fidelity pcr system
    Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
    Expand High Fidelity Pcr System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2021-07
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    takara expand high fidelity
    Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
    Expand High Fidelity, supplied by takara, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Journal: Journal of Zhejiang University. Science. B

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    doi: 10.1631/jzus.B1500008

    Figure Lengend Snippet: Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Article Snippet: The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China).

    Techniques: Polymerase Chain Reaction, Amplification, Isolation, Purification

    Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Journal: Journal of Zhejiang University. Science. B

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    doi: 10.1631/jzus.B1500008

    Figure Lengend Snippet: Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Article Snippet: The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China).

    Techniques: Recombinant, Plasmid Preparation, Positron Emission Tomography, Polymerase Chain Reaction, Amplification, Marker

    Gel electrophoresis of PCR products of α-gal gene. (a) (lane 1–3 represent PCR products of α-gal gene, M represents DNA Marker DL2000) and amino acid sequences analysis of AglB by BLASTn.

    Journal: PLoS ONE

    Article Title: Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties

    doi: 10.1371/journal.pone.0235687

    Figure Lengend Snippet: Gel electrophoresis of PCR products of α-gal gene. (a) (lane 1–3 represent PCR products of α-gal gene, M represents DNA Marker DL2000) and amino acid sequences analysis of AglB by BLASTn.

    Article Snippet: The Expand High Fidelity PCR System with PrimeSTAR® HS DNA Polymerase (Takara, Dalian, China) was used to clone gene aga1 (Accession number: CP020457) with oligonucleotides: 5’- CCG GAATTC ATGAA CCACGAACTAATCAC-3′ containing a EcoR I site (underlined) and 5′-CCG CTCGAG TTAATTCCGTACACTGTTTG-3′ containing a Xho I site (underlined).

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Marker