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TaKaRa expand high fidelity pcr system
Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
Expand High Fidelity Pcr System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand high fidelity pcr system/product/TaKaRa
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
expand high fidelity pcr system - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *"

Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

Journal: Journal of Zhejiang University. Science. B

doi: 10.1631/jzus.B1500008

Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
Figure Legend Snippet: Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

Techniques Used: Polymerase Chain Reaction, Amplification, Isolation, Purification

Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker
Figure Legend Snippet: Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

Techniques Used: Recombinant, Plasmid Preparation, Positron Emission Tomography, Polymerase Chain Reaction, Amplification, Marker

Related Articles

Polymerase Chain Reaction:

Article Title: Role of toll-like receptor 4 on the immune escape of human oral squamous cell carcinoma and resistance of cisplatin-induced apoptosis
Article Snippet: .. PCR was performed using an Expand High Fidelity PCR System (TaKaRa, Shiga, Japan). .. Flow cytometry TLR4 expression in cells was evaluated by flow cytometry as follows: cells were collected and then labeled with the APC-labeled mouse anti-human TLR4 antibody (eBioscience, CA, USA) for 30 min at 4°C.

Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *
Article Snippet: .. The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China). .. Horseradish peroxidase (HRP)-labelled goat anti-mouse immunoglobulin G (IgG), Coomassie Brilliant Blue G-250, acetylthiocholine iodide, propionylthiocholine iodide, Freund’s complete adjuvant, Freund’s incomplete adjuvant, and bovine serum albumin (BSA, with a molecular weight (MW) of 67 000) were purchased from Sigma-Aldrich (Steinheim, Germany).

Article Title: Expression, Purification, and Characterization of Cu/ZnSOD from Panax Ginseng
Article Snippet: .. All restriction enzymes and the Expand High Fidelity PCR system were purchased from TakaRa. .. The sequences of the PCR products used for cloning were confirmed by sequencing at BGI Co., Ltd (Beijing, China).

Positron Emission Tomography:

Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *
Article Snippet: .. The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China). .. Horseradish peroxidase (HRP)-labelled goat anti-mouse immunoglobulin G (IgG), Coomassie Brilliant Blue G-250, acetylthiocholine iodide, propionylthiocholine iodide, Freund’s complete adjuvant, Freund’s incomplete adjuvant, and bovine serum albumin (BSA, with a molecular weight (MW) of 67 000) were purchased from Sigma-Aldrich (Steinheim, Germany).

Plasmid Preparation:

Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *
Article Snippet: .. The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China). .. Horseradish peroxidase (HRP)-labelled goat anti-mouse immunoglobulin G (IgG), Coomassie Brilliant Blue G-250, acetylthiocholine iodide, propionylthiocholine iodide, Freund’s complete adjuvant, Freund’s incomplete adjuvant, and bovine serum albumin (BSA, with a molecular weight (MW) of 67 000) were purchased from Sigma-Aldrich (Steinheim, Germany).

Purification:

Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *
Article Snippet: .. The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China). .. Horseradish peroxidase (HRP)-labelled goat anti-mouse immunoglobulin G (IgG), Coomassie Brilliant Blue G-250, acetylthiocholine iodide, propionylthiocholine iodide, Freund’s complete adjuvant, Freund’s incomplete adjuvant, and bovine serum albumin (BSA, with a molecular weight (MW) of 67 000) were purchased from Sigma-Aldrich (Steinheim, Germany).

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    TaKaRa expand high fidelity pcr system
    Results of <t>PCR</t> amplification (a) Result of RNA isolation. Lane 1: 100 bp <t>DNA</t> ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder
    Expand High Fidelity Pcr System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/TaKaRa
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Journal: Journal of Zhejiang University. Science. B

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    doi: 10.1631/jzus.B1500008

    Figure Lengend Snippet: Results of PCR amplification (a) Result of RNA isolation. Lane 1: 100 bp DNA ladder;Lane 2: total RNA. (b, c) Result of PCR using cDNA as template. (b) Lane 1: 100 bp DNA ladder; Lane 2: PCR product which indicates gene segments of about 200, 350,and 700 bp. (c) Lane 1: purified PCR product of about 700 bp; Lane 2: 100 bp DNA ladder

    Article Snippet: The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China).

    Techniques: Polymerase Chain Reaction, Amplification, Isolation, Purification

    Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Journal: Journal of Zhejiang University. Science. B

    Article Title: Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna *

    doi: 10.1631/jzus.B1500008

    Figure Lengend Snippet: Identification of the recombinant plasmid pET-29a(+)- ChE by PCR (a) and by restriction enzyme EcoR I and Sal I (b) (a) Lanes 1 and 2: result of PCR amplification using trans-fected cells as template; Lane 3: 100 bp DNA Ladder.(b) Lane 1: digested pET-29a(+)- ChE by EcoR I and Sal I;Lane 2: 250 bp DNA ladder marker

    Article Snippet: The pMD19-T vector, PET-29a(+) plasmid, Expand High Fidelity PCR system, HisTALON™ Gravity Columns Purification Kit, DNA Recovery Kit, and all of the restriction enzymes were purchased from TaKaRa (Dalian, China).

    Techniques: Recombinant, Plasmid Preparation, Positron Emission Tomography, Polymerase Chain Reaction, Amplification, Marker