expand high fidelity pcr system  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Millipore expand high fidelity pcr system
    Mechanisms of <t>GILZ</t> downregulation upon TLR activation. (A,B) AMs pretreated with BAY-11-7082 (BAY82, 5 μM), BAY-11-7085 (BAY85, 5 μM), or the solvent control DMSO (0.1%) for 1 h, followed by treatment with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), Poly(I:C) (PIC, 10 μg/mL), or medium (Co) for 4 h. GILZ expression was determined by Western blot. Tubulin served as a loading control. (A) Representative blot. (B) GILZ signal intensities were quantified and normalized to tubulin values ( n = 7). Values for unstimulated DMSO controls were set as 100%. (C,D) After preincubation with BAY-11-7082 or BAY-11-7085 (5 μM, 1 h), solvent (0.1% DMSO) or medium only (Co), AMs were treated with LPS (100 ng/mL) for 2 h. GILZ and ZFP36 mRNA expression was determined by <t>qRT-PCR</t> using ACTB as a housekeeping gene ( n = 3, duplicates). (E) AMs were either left untreated (Co) or treated with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), or Poly(I:C) (PIC, 10 μg/mL) for 4 h. TTP levels were determined by Western blot using tubulin as a loading control. GILZ signal intensities were normalized to tubulin and are shown as a percentage of untreated cells ( n = 2, triplicates). (F) The number of miRNAs predicted to target GILZ, CXCL8, IL6 , and TNF was assessed via the microRNA Data Integration Portal (mirDIP, accession date 02/02/2018). (G) AMs were either left untreated (Co) or treated with Poly(I:C) (PIC, 10 μg/mL), aurintricarboxylic acid (ATA, 25 μM), or a combination of both for 8 h. GILZ expression was quantified by Western blot. Signal intensities were normalized to tubulin and expressed as a percentage of untreated cells ( n = 3). * p
    Expand High Fidelity Pcr System, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages"

    Article Title: Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.03111

    Mechanisms of GILZ downregulation upon TLR activation. (A,B) AMs pretreated with BAY-11-7082 (BAY82, 5 μM), BAY-11-7085 (BAY85, 5 μM), or the solvent control DMSO (0.1%) for 1 h, followed by treatment with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), Poly(I:C) (PIC, 10 μg/mL), or medium (Co) for 4 h. GILZ expression was determined by Western blot. Tubulin served as a loading control. (A) Representative blot. (B) GILZ signal intensities were quantified and normalized to tubulin values ( n = 7). Values for unstimulated DMSO controls were set as 100%. (C,D) After preincubation with BAY-11-7082 or BAY-11-7085 (5 μM, 1 h), solvent (0.1% DMSO) or medium only (Co), AMs were treated with LPS (100 ng/mL) for 2 h. GILZ and ZFP36 mRNA expression was determined by qRT-PCR using ACTB as a housekeeping gene ( n = 3, duplicates). (E) AMs were either left untreated (Co) or treated with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), or Poly(I:C) (PIC, 10 μg/mL) for 4 h. TTP levels were determined by Western blot using tubulin as a loading control. GILZ signal intensities were normalized to tubulin and are shown as a percentage of untreated cells ( n = 2, triplicates). (F) The number of miRNAs predicted to target GILZ, CXCL8, IL6 , and TNF was assessed via the microRNA Data Integration Portal (mirDIP, accession date 02/02/2018). (G) AMs were either left untreated (Co) or treated with Poly(I:C) (PIC, 10 μg/mL), aurintricarboxylic acid (ATA, 25 μM), or a combination of both for 8 h. GILZ expression was quantified by Western blot. Signal intensities were normalized to tubulin and expressed as a percentage of untreated cells ( n = 3). * p
    Figure Legend Snippet: Mechanisms of GILZ downregulation upon TLR activation. (A,B) AMs pretreated with BAY-11-7082 (BAY82, 5 μM), BAY-11-7085 (BAY85, 5 μM), or the solvent control DMSO (0.1%) for 1 h, followed by treatment with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), Poly(I:C) (PIC, 10 μg/mL), or medium (Co) for 4 h. GILZ expression was determined by Western blot. Tubulin served as a loading control. (A) Representative blot. (B) GILZ signal intensities were quantified and normalized to tubulin values ( n = 7). Values for unstimulated DMSO controls were set as 100%. (C,D) After preincubation with BAY-11-7082 or BAY-11-7085 (5 μM, 1 h), solvent (0.1% DMSO) or medium only (Co), AMs were treated with LPS (100 ng/mL) for 2 h. GILZ and ZFP36 mRNA expression was determined by qRT-PCR using ACTB as a housekeeping gene ( n = 3, duplicates). (E) AMs were either left untreated (Co) or treated with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), or Poly(I:C) (PIC, 10 μg/mL) for 4 h. TTP levels were determined by Western blot using tubulin as a loading control. GILZ signal intensities were normalized to tubulin and are shown as a percentage of untreated cells ( n = 2, triplicates). (F) The number of miRNAs predicted to target GILZ, CXCL8, IL6 , and TNF was assessed via the microRNA Data Integration Portal (mirDIP, accession date 02/02/2018). (G) AMs were either left untreated (Co) or treated with Poly(I:C) (PIC, 10 μg/mL), aurintricarboxylic acid (ATA, 25 μM), or a combination of both for 8 h. GILZ expression was quantified by Western blot. Signal intensities were normalized to tubulin and expressed as a percentage of untreated cells ( n = 3). * p

    Techniques Used: Activation Assay, Affinity Magnetic Separation, Expressing, Western Blot, Quantitative RT-PCR

    2) Product Images from "Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages"

    Article Title: Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.03111

    Mechanisms of GILZ downregulation upon TLR activation. (A,B) AMs pretreated with BAY-11-7082 (BAY82, 5 μM), BAY-11-7085 (BAY85, 5 μM), or the solvent control DMSO (0.1%) for 1 h, followed by treatment with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), Poly(I:C) (PIC, 10 μg/mL), or medium (Co) for 4 h. GILZ expression was determined by Western blot. Tubulin served as a loading control. (A) Representative blot. (B) GILZ signal intensities were quantified and normalized to tubulin values ( n = 7). Values for unstimulated DMSO controls were set as 100%. (C,D) After preincubation with BAY-11-7082 or BAY-11-7085 (5 μM, 1 h), solvent (0.1% DMSO) or medium only (Co), AMs were treated with LPS (100 ng/mL) for 2 h. GILZ and ZFP36 mRNA expression was determined by qRT-PCR using ACTB as a housekeeping gene ( n = 3, duplicates). (E) AMs were either left untreated (Co) or treated with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), or Poly(I:C) (PIC, 10 μg/mL) for 4 h. TTP levels were determined by Western blot using tubulin as a loading control. GILZ signal intensities were normalized to tubulin and are shown as a percentage of untreated cells ( n = 2, triplicates). (F) The number of miRNAs predicted to target GILZ, CXCL8, IL6 , and TNF was assessed via the microRNA Data Integration Portal (mirDIP, accession date 02/02/2018). (G) AMs were either left untreated (Co) or treated with Poly(I:C) (PIC, 10 μg/mL), aurintricarboxylic acid (ATA, 25 μM), or a combination of both for 8 h. GILZ expression was quantified by Western blot. Signal intensities were normalized to tubulin and expressed as a percentage of untreated cells ( n = 3). * p
    Figure Legend Snippet: Mechanisms of GILZ downregulation upon TLR activation. (A,B) AMs pretreated with BAY-11-7082 (BAY82, 5 μM), BAY-11-7085 (BAY85, 5 μM), or the solvent control DMSO (0.1%) for 1 h, followed by treatment with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), Poly(I:C) (PIC, 10 μg/mL), or medium (Co) for 4 h. GILZ expression was determined by Western blot. Tubulin served as a loading control. (A) Representative blot. (B) GILZ signal intensities were quantified and normalized to tubulin values ( n = 7). Values for unstimulated DMSO controls were set as 100%. (C,D) After preincubation with BAY-11-7082 or BAY-11-7085 (5 μM, 1 h), solvent (0.1% DMSO) or medium only (Co), AMs were treated with LPS (100 ng/mL) for 2 h. GILZ and ZFP36 mRNA expression was determined by qRT-PCR using ACTB as a housekeeping gene ( n = 3, duplicates). (E) AMs were either left untreated (Co) or treated with LPS (100 ng/mL), Pam 3 CSK 4 (Pam, 1 μg/mL), or Poly(I:C) (PIC, 10 μg/mL) for 4 h. TTP levels were determined by Western blot using tubulin as a loading control. GILZ signal intensities were normalized to tubulin and are shown as a percentage of untreated cells ( n = 2, triplicates). (F) The number of miRNAs predicted to target GILZ, CXCL8, IL6 , and TNF was assessed via the microRNA Data Integration Portal (mirDIP, accession date 02/02/2018). (G) AMs were either left untreated (Co) or treated with Poly(I:C) (PIC, 10 μg/mL), aurintricarboxylic acid (ATA, 25 μM), or a combination of both for 8 h. GILZ expression was quantified by Western blot. Signal intensities were normalized to tubulin and expressed as a percentage of untreated cells ( n = 3). * p

    Techniques Used: Activation Assay, Affinity Magnetic Separation, Expressing, Western Blot, Quantitative RT-PCR

    Related Articles

    Amplification:

    Article Title: Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages
    Article Snippet: .. Human GILZ 3′UTR cDNA was amplified using the Expand High fidelity PCR System (Sigma-Aldrich, # 11732641001) and the following primers: 5′-GCC TAC TAG TGC AGA GCC ACT AAA CTT G-3′ and 5′-AAT AGA GCT CAC TCT CAC AAA ACC CGC TAC-3′. .. The SacI/SpeI digested PCR product was inserted into the respective cloning site of pMIR-REPORT (Ambion, #AM5795).

    Article Title: Mechanisms regulating PD-L1 expression on tumor and immune cells
    Article Snippet: .. These two regions were amplified using an Expand TM High Fidelity PCR system (Sigma, catalog # 11732650001). .. Amplified PCR products were purified by a QIAquick PCR Purification kit (Qiagen, catalog # 28104) and sent to the Johns Hopkins University Core Facility for Sanger sequencing.

    Article Title: Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy
    Article Snippet: .. Twenty microlitres of Klenow product was amplified by the Expand High Fidelity PCR System (Sigma Aldrich S.R.L., Milan, Italy) using FR20RV-T primer complementary to the sequence tag. .. Five microlitres of the PCR product was analysed on a 1% agarose gel.

    Purification:

    Article Title: Hemolysis, Toxicity, and Randomly Amplified Polymorphic DNA Analysis of Stachybotrys chartarum Strains
    Article Snippet: .. The RAPD reaction was done in a total volume of 50 μl containing 0.2 μl of a 50 nM solution of the R28 primer, 1 μl of PCR nucleotide mix (1581295; Boehringer, Indianapolis, Ind.), 0.3 μl of Expand High Fidelity PCR System DNA polymerase (84367521; Boehringer), 5 μl of 10× PCR buffer with MgCl (83073623; Boehringer), 5 μl of a sterile bovine serum albumin (Fraction V; Sigma) solution containing 2 mg of water per ml, 7 μl of a 50% solution of glycerol in water, 26.5 μl of sterile deionized water, and 5 μl of the purified DNA solution. .. The PCR was conducted at 92°C for 1 min, followed by 30 cycles of denaturation at 92°C for 45 s, annealing at 34°C for 60 s, and extension at 72°C for 90 s; then, the final extension was done at 72°C for 10 min in a thermal cycler (PTC-200; MJ Research, Watertown, Mass.).

    Polymerase Chain Reaction:

    Article Title: P450-Catalyzed Tailoring Steps in Leinamycin Biosynthesis Featuring Regio- and Stereoselective Hydroxylations and Substrate Promiscuities
    Article Snippet: .. The Expand High Fidelity PCR System and DIG-High Prime DNA Labeling and Detection Starter Kit were purchased from Sigma and used following the protocols provided by the manufacturer. .. Restriction endonucleases and T4 DNA ligase were purchased from New England Biolabs.

    Article Title: sRNA Target Prediction Organizing Tool (SPOT) Integrates Computational and Experimental Data To Facilitate Functional Characterization of Bacterial Small RNAs
    Article Snippet: .. PCR products were generated using the Expand high-fidelity PCR system (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions. .. All mutations were verified by amplifying PCR fragments using GoTaq polymerase (Promega, Madison, WI) and sequencing.

    Article Title: Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector
    Article Snippet: .. The samples were then centrifuged for 5 min at maximum speed to remove cell debris, and the supernatant was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). .. The PCR products were purified using the QIAquick PCR Purification kit (Qiagen), quantified by NanoDrop (NanoDrop Technologies Inc.), and sequenced by Sanger sequencing.

    Article Title: Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages
    Article Snippet: .. Human GILZ 3′UTR cDNA was amplified using the Expand High fidelity PCR System (Sigma-Aldrich, # 11732641001) and the following primers: 5′-GCC TAC TAG TGC AGA GCC ACT AAA CTT G-3′ and 5′-AAT AGA GCT CAC TCT CAC AAA ACC CGC TAC-3′. .. The SacI/SpeI digested PCR product was inserted into the respective cloning site of pMIR-REPORT (Ambion, #AM5795).

    Article Title: Mechanisms regulating PD-L1 expression on tumor and immune cells
    Article Snippet: .. These two regions were amplified using an Expand TM High Fidelity PCR system (Sigma, catalog # 11732650001). .. Amplified PCR products were purified by a QIAquick PCR Purification kit (Qiagen, catalog # 28104) and sent to the Johns Hopkins University Core Facility for Sanger sequencing.

    Article Title: Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy
    Article Snippet: .. Twenty microlitres of Klenow product was amplified by the Expand High Fidelity PCR System (Sigma Aldrich S.R.L., Milan, Italy) using FR20RV-T primer complementary to the sequence tag. .. Five microlitres of the PCR product was analysed on a 1% agarose gel.

    Article Title: Hemolysis, Toxicity, and Randomly Amplified Polymorphic DNA Analysis of Stachybotrys chartarum Strains
    Article Snippet: .. The RAPD reaction was done in a total volume of 50 μl containing 0.2 μl of a 50 nM solution of the R28 primer, 1 μl of PCR nucleotide mix (1581295; Boehringer, Indianapolis, Ind.), 0.3 μl of Expand High Fidelity PCR System DNA polymerase (84367521; Boehringer), 5 μl of 10× PCR buffer with MgCl (83073623; Boehringer), 5 μl of a sterile bovine serum albumin (Fraction V; Sigma) solution containing 2 mg of water per ml, 7 μl of a 50% solution of glycerol in water, 26.5 μl of sterile deionized water, and 5 μl of the purified DNA solution. .. The PCR was conducted at 92°C for 1 min, followed by 30 cycles of denaturation at 92°C for 45 s, annealing at 34°C for 60 s, and extension at 72°C for 90 s; then, the final extension was done at 72°C for 10 min in a thermal cycler (PTC-200; MJ Research, Watertown, Mass.).

    Generated:

    Article Title: sRNA Target Prediction Organizing Tool (SPOT) Integrates Computational and Experimental Data To Facilitate Functional Characterization of Bacterial Small RNAs
    Article Snippet: .. PCR products were generated using the Expand high-fidelity PCR system (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions. .. All mutations were verified by amplifying PCR fragments using GoTaq polymerase (Promega, Madison, WI) and sequencing.

    DNA Labeling:

    Article Title: P450-Catalyzed Tailoring Steps in Leinamycin Biosynthesis Featuring Regio- and Stereoselective Hydroxylations and Substrate Promiscuities
    Article Snippet: .. The Expand High Fidelity PCR System and DIG-High Prime DNA Labeling and Detection Starter Kit were purchased from Sigma and used following the protocols provided by the manufacturer. .. Restriction endonucleases and T4 DNA ligase were purchased from New England Biolabs.

    Sequencing:

    Article Title: Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy
    Article Snippet: .. Twenty microlitres of Klenow product was amplified by the Expand High Fidelity PCR System (Sigma Aldrich S.R.L., Milan, Italy) using FR20RV-T primer complementary to the sequence tag. .. Five microlitres of the PCR product was analysed on a 1% agarose gel.

    Activated Clotting Time Assay:

    Article Title: Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages
    Article Snippet: .. Human GILZ 3′UTR cDNA was amplified using the Expand High fidelity PCR System (Sigma-Aldrich, # 11732641001) and the following primers: 5′-GCC TAC TAG TGC AGA GCC ACT AAA CTT G-3′ and 5′-AAT AGA GCT CAC TCT CAC AAA ACC CGC TAC-3′. .. The SacI/SpeI digested PCR product was inserted into the respective cloning site of pMIR-REPORT (Ambion, #AM5795).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Millipore expand high fidelity pcr system
    Expand High Fidelity Pcr System, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand high fidelity pcr system/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand high fidelity pcr system - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    Image Search Results