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Atlanta Biologicals exosome depleted fbs
Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either <t>exosome-depleted</t> <t>FBS</t> (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).
Exosome Depleted Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Article Title: Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone

Journal: PLoS ONE

doi: 10.1371/journal.pone.0165326

Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either exosome-depleted FBS (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).
Figure Legend Snippet: Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either exosome-depleted FBS (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).

Techniques Used: Purification, Western Blot, Isolation

Related Articles

Purification:

Article Title: Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone
Article Snippet: .. In some experiments, purified human plasma fibronectin (#F0895, Sigma-Aldrich) or exosome-depleted FBS (#S11550, Atlanta Biologicals, Lawrenceville, GA; FBS spun overnight at 100,000 g ) was added to cleared supernatant for a final concentration of 2.5 μg/ml or 10% v:v, respectively. .. Cleared supernatant was spun at 10,000 g for 40min at 4°C with a Beckman SW28 rotor.

Concentration Assay:

Article Title: Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone
Article Snippet: .. In some experiments, purified human plasma fibronectin (#F0895, Sigma-Aldrich) or exosome-depleted FBS (#S11550, Atlanta Biologicals, Lawrenceville, GA; FBS spun overnight at 100,000 g ) was added to cleared supernatant for a final concentration of 2.5 μg/ml or 10% v:v, respectively. .. Cleared supernatant was spun at 10,000 g for 40min at 4°C with a Beckman SW28 rotor.

Isolation:

Article Title: HCV-associated exosomes promote myeloid-derived suppressor cell expansion via inhibiting miR-124 to regulate T follicular cell differentiation and function
Article Snippet: CD33+ myeloid cells were further isolated or depleted, depending on the experimental requirements, from PBMCs using the CD33+ Cell Isolation Kit and a MidiMACS™ Separator (Miltenyi Biotec Inc., Auburn, CA). .. The isolated cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA), 100 IU/ml penicillin and 2 mM l -glutamine (Thermo Scientific, Logan, Utah) at 37 °C and 5% CO2 atmosphere. .. Co-culture of cells with or without exosomes was carried out as described in the Results.

Article Title: HCV-associated exosomes promote myeloid-derived suppressor cell expansion via inhibiting miR-124 to regulate T follicular cell differentiation and function
Article Snippet: CD33+ myeloid cells were further isolated or depleted, depending on the experimental requirements, from PBMCs using the CD33+ Cell Isolation Kit and a MidiMACS™ Separator (Miltenyi Biotec Inc., Auburn, CA). .. The isolated cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA), 100 IU/ml penicillin and 2 mM l -glutamine (Thermo Scientific, Logan, Utah) at 37 °C and 5% CO2 atmosphere. .. Co-culture of cells with or without exosomes was carried out as described in the Results.

Article Title: Exosome is a Mechanism of Intercellular Drug Transfer: Application of Quantitative Pharmacology
Article Snippet: MCF7 and LM2 cells were maintained in DMEM (Mediatech, VA), and A2780 and OVCAR4 cells in RPMI-1640 (ATCC). .. The medium for cell growth (Growth Medium) was supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, GA), whereas the medium for exosome isolation (Conditioned Medium) was supplemented with 10% exosome-depleted-FBS (System Biosciences, CA). .. All medium contained 100 IU/mL penicillin and 100 μg/mL streptomycin (Mediatech, VA).

Cell Culture:

Article Title: HCV-associated exosomes promote myeloid-derived suppressor cell expansion via inhibiting miR-124 to regulate T follicular cell differentiation and function
Article Snippet: CD33+ myeloid cells were further isolated or depleted, depending on the experimental requirements, from PBMCs using the CD33+ Cell Isolation Kit and a MidiMACS™ Separator (Miltenyi Biotec Inc., Auburn, CA). .. The isolated cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA), 100 IU/ml penicillin and 2 mM l -glutamine (Thermo Scientific, Logan, Utah) at 37 °C and 5% CO2 atmosphere. .. Co-culture of cells with or without exosomes was carried out as described in the Results.

Article Title: HCV-associated exosomes promote myeloid-derived suppressor cell expansion via inhibiting miR-124 to regulate T follicular cell differentiation and function
Article Snippet: CD33+ myeloid cells were further isolated or depleted, depending on the experimental requirements, from PBMCs using the CD33+ Cell Isolation Kit and a MidiMACS™ Separator (Miltenyi Biotec Inc., Auburn, CA). .. The isolated cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA), 100 IU/ml penicillin and 2 mM l -glutamine (Thermo Scientific, Logan, Utah) at 37 °C and 5% CO2 atmosphere. .. Co-culture of cells with or without exosomes was carried out as described in the Results.

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    Atlanta Biologicals exosome depleted fbs
    Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either <t>exosome-depleted</t> <t>FBS</t> (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).
    Exosome Depleted Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosome depleted fbs/product/Atlanta Biologicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exosome depleted fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either exosome-depleted FBS (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).

    Journal: PLoS ONE

    Article Title: Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone

    doi: 10.1371/journal.pone.0165326

    Figure Lengend Snippet: Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either exosome-depleted FBS (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).

    Article Snippet: In some experiments, purified human plasma fibronectin (#F0895, Sigma-Aldrich) or exosome-depleted FBS (#S11550, Atlanta Biologicals, Lawrenceville, GA; FBS spun overnight at 100,000 g ) was added to cleared supernatant for a final concentration of 2.5 μg/ml or 10% v:v, respectively.

    Techniques: Purification, Western Blot, Isolation