ec exo iii  (New England Biolabs)


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    Name:
    Exonuclease III E coli
    Description:
    Exonuclease III E coli 25 000 units
    Catalog Number:
    M0206L
    Price:
    248
    Category:
    Exonucleases
    Size:
    25 000 units
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    Structured Review

    New England Biolabs ec exo iii
    Exonuclease III E coli
    Exonuclease III E coli 25 000 units
    https://www.bioz.com/result/ec exo iii/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ec exo iii - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "The RNA polymerase bridge helix YFI motif in catalysis, fidelity and translocation"

    Article Title: The RNA polymerase bridge helix YFI motif in catalysis, fidelity and translocation

    Journal: Biochimica et biophysica acta

    doi: 10.1016/j.bbagrm.2012.11.005

    RNAP C14 TEC downstream border Exo III mapping indicating the slow post → pre and pre → backtracked transitions. A) Protocol and schematic. In vitro assembled Ec RNAP G9 TECs were extended to G10 and washed. After releasing TECs from beads,
    Figure Legend Snippet: RNAP C14 TEC downstream border Exo III mapping indicating the slow post → pre and pre → backtracked transitions. A) Protocol and schematic. In vitro assembled Ec RNAP G9 TECs were extended to G10 and washed. After releasing TECs from beads,

    Techniques Used: In Vitro

    2) Product Images from "Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification"

    Article Title: Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2015.01385

    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.
    Figure Legend Snippet: Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Techniques Used: Amplification, Polymerase Chain Reaction, Ligation, Sequencing, Hybridization, DNA Synthesis, Nucleic Acid Electrophoresis, SYBR Green Assay

    3) Product Images from "Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification"

    Article Title: Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2015.01385

    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.
    Figure Legend Snippet: Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Techniques Used: Amplification, Polymerase Chain Reaction, Ligation, Sequencing, Hybridization, DNA Synthesis, Nucleic Acid Electrophoresis, SYBR Green Assay

    Related Articles

    Labeling:

    Article Title: The mechanism of gap creation by a multifunctional nuclease during base excision repair
    Article Snippet: .. Protein and DNA preparationWT ExoIII was purchased from New England Biolabs (NEB) (catalog #M0206S), and all mutants and fluorescently labeled WT ExoIII were purified (Supplementary Materials). ..

    Purification:

    Article Title: The mechanism of gap creation by a multifunctional nuclease during base excision repair
    Article Snippet: .. Protein and DNA preparationWT ExoIII was purchased from New England Biolabs (NEB) (catalog #M0206S), and all mutants and fluorescently labeled WT ExoIII were purified (Supplementary Materials). ..

    Article Title: A small-molecule chemical interface for molecular programs
    Article Snippet: .. The thermophilic 5′ → 3′ exonuclease ttRecJ was purified in the laboratory, stored in Diluent A (NEB)+ 0.1% Triton X-100 at a concentration of 1.53 μM and used at 23 nM. ..

    Incubation:

    Article Title: CaBagE: A Cas9-based Back ground Elimination strategy for targeted, long-read DNA sequencing
    Article Snippet: .. Exonuclease digestionImmediately following Cas9 digestion, 260 total units of exonucleases (Exo I ([40U] NEB M0293), Exo III ([200U] NEB M0206), Lambda ([20U] NEB M0262]) diluted in 1X CutSmart buffer to 10μL were added to the reaction for a final reaction volume 50uL and incubated at 37°C for two hours, followed by heat inactivation at 80°C for 20 minutes. ..

    Concentration Assay:

    Article Title: A small-molecule chemical interface for molecular programs
    Article Snippet: .. The thermophilic 5′ → 3′ exonuclease ttRecJ was purified in the laboratory, stored in Diluent A (NEB)+ 0.1% Triton X-100 at a concentration of 1.53 μM and used at 23 nM. ..

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    New England Biolabs exonuclease iii e coli
    Performance of o2n-seq for detecting mutations with 1% and 0.1% allele frequency. ( a , b ) Sensitivity and FPR of mutation detection of o2n-seq <t>(three</t> experimental replicates, orange), Cir-seq (three experimental replicates, blue) and o2n-seq after filtering with frequency (o2n-seq-f, green) under different CSs criteria for the 1:100 mixture of E. coli (means±s.d.). Two-tailed Student's t -test was used for statistical analysis. ( c ) Mutation frequency distribution of FP and TP variants detected by o2n-seq under different CSs (1 × and 2 × ) for the 1:100 mixture of E. coli . 3 × -5 × CSs were showed in Supplementary Fig. 5 . ( d ) MAFs of TP mutations detected by o2n-seq for the 1:100 mixture of E. coli . The MAFs of three experimental replicates was plotted. The dashed horizontal line indicates the theoretical MAF (0.99%). ( e , f ) Sensitivity and FPR of mutation detection of o2n-seq by different CSs criteria (3 × −9 × ) under different total CSs coverage (5,000–25,000 × ) for the 1:1,000 mix of phix174 . The results of the other experimental replicate were shown in Supplementary Fig. 6 . Dash lines were used to display the overlapped results better.
    Exonuclease Iii E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii e coli/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease iii e coli - by Bioz Stars, 2021-09
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    Performance of o2n-seq for detecting mutations with 1% and 0.1% allele frequency. ( a , b ) Sensitivity and FPR of mutation detection of o2n-seq (three experimental replicates, orange), Cir-seq (three experimental replicates, blue) and o2n-seq after filtering with frequency (o2n-seq-f, green) under different CSs criteria for the 1:100 mixture of E. coli (means±s.d.). Two-tailed Student's t -test was used for statistical analysis. ( c ) Mutation frequency distribution of FP and TP variants detected by o2n-seq under different CSs (1 × and 2 × ) for the 1:100 mixture of E. coli . 3 × -5 × CSs were showed in Supplementary Fig. 5 . ( d ) MAFs of TP mutations detected by o2n-seq for the 1:100 mixture of E. coli . The MAFs of three experimental replicates was plotted. The dashed horizontal line indicates the theoretical MAF (0.99%). ( e , f ) Sensitivity and FPR of mutation detection of o2n-seq by different CSs criteria (3 × −9 × ) under different total CSs coverage (5,000–25,000 × ) for the 1:1,000 mix of phix174 . The results of the other experimental replicate were shown in Supplementary Fig. 6 . Dash lines were used to display the overlapped results better.

    Journal: Nature Communications

    Article Title: Ultrasensitive and high-efficiency screen of de novo low-frequency mutations by o2n-seq

    doi: 10.1038/ncomms15335

    Figure Lengend Snippet: Performance of o2n-seq for detecting mutations with 1% and 0.1% allele frequency. ( a , b ) Sensitivity and FPR of mutation detection of o2n-seq (three experimental replicates, orange), Cir-seq (three experimental replicates, blue) and o2n-seq after filtering with frequency (o2n-seq-f, green) under different CSs criteria for the 1:100 mixture of E. coli (means±s.d.). Two-tailed Student's t -test was used for statistical analysis. ( c ) Mutation frequency distribution of FP and TP variants detected by o2n-seq under different CSs (1 × and 2 × ) for the 1:100 mixture of E. coli . 3 × -5 × CSs were showed in Supplementary Fig. 5 . ( d ) MAFs of TP mutations detected by o2n-seq for the 1:100 mixture of E. coli . The MAFs of three experimental replicates was plotted. The dashed horizontal line indicates the theoretical MAF (0.99%). ( e , f ) Sensitivity and FPR of mutation detection of o2n-seq by different CSs criteria (3 × −9 × ) under different total CSs coverage (5,000–25,000 × ) for the 1:1,000 mix of phix174 . The results of the other experimental replicate were shown in Supplementary Fig. 6 . Dash lines were used to display the overlapped results better.

    Article Snippet: Subsequently, 1 μl Exonuclease I (NEB, M0293S) and 1 μl Exonuclease III (NEB, M0206S) were added into the reaction and incubated at 37 °C for 1 h. The enzymes were inactivated at 80 °C for another 20 min.

    Techniques: Mutagenesis, Two Tailed Test

    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Journal: Frontiers in Microbiology

    Article Title: Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification

    doi: 10.3389/fmicb.2015.01385

    Figure Lengend Snippet: Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Article Snippet: The exonucleolysis mix consisted of 0.5 μL Exo I (New England BioLabs, Ipswich, MA, USA), 1 μL 10 × Exo I Buffer (New England BioLabs), 0.2 μL Exo III (New England BioLabs), 1 μL 10 × Exo III Buffer (New England BioLabs), and 7.3 μL ultra-pure water.

    Techniques: Amplification, Polymerase Chain Reaction, Ligation, Sequencing, Hybridization, DNA Synthesis, Nucleic Acid Electrophoresis, SYBR Green Assay

    Footprint analysis of IS 1535 deletion substrate LE(v54-20v) containing the minimal transposon sequences required for efficient PEC assembly. ( A ) DNase I footprints of PEC assembly reactions on 5′- 32 P-labeled bottom and top strands of LE(v54-20v). TnpA concentrations were from 4 to 128 nM in 2-fold increasing amounts. Shaded rectangles on the left of the gels denote the positions of transposon sequences; coordinates labeled with v are vector sequences with vH being the equivalent locations of host DNA. The bars on the right of the gels denote regions of significant changes in DNase I reactivity by TnpA with dashes indicating weakly protected regions. ( B ) Exonuclease III delineated boundaries of TnpA binding. TnpA concentrations are the same as in panel A. ( C ) Summary of DNase I (strongly protected regions, blue) and Exo III digestion boundaries on the LE(v54-20v) sequence. Small letters denote vector sequence.

    Journal: eLife

    Article Title: Multiple serine transposase dimers assemble the transposon-end synaptic complex during IS607-family transposition

    doi: 10.7554/eLife.39611

    Figure Lengend Snippet: Footprint analysis of IS 1535 deletion substrate LE(v54-20v) containing the minimal transposon sequences required for efficient PEC assembly. ( A ) DNase I footprints of PEC assembly reactions on 5′- 32 P-labeled bottom and top strands of LE(v54-20v). TnpA concentrations were from 4 to 128 nM in 2-fold increasing amounts. Shaded rectangles on the left of the gels denote the positions of transposon sequences; coordinates labeled with v are vector sequences with vH being the equivalent locations of host DNA. The bars on the right of the gels denote regions of significant changes in DNase I reactivity by TnpA with dashes indicating weakly protected regions. ( B ) Exonuclease III delineated boundaries of TnpA binding. TnpA concentrations are the same as in panel A. ( C ) Summary of DNase I (strongly protected regions, blue) and Exo III digestion boundaries on the LE(v54-20v) sequence. Small letters denote vector sequence.

    Article Snippet: After 60 min incubation at 37°C with TnpAIS1535 , DNase I (0.02 u, Thermo Fisher, Waltham, MA) or Exonuclease III (10 u, NEB, Ipswich, MA) was added for 30 s or 5 min, respectively.

    Techniques: Labeling, Plasmid Preparation, Binding Assay, Sequencing