exonuclease i e coli (New England Biolabs)
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Exonuclease I (E.coli) - 1
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New England Biolabs
exonuclease i e coli
https://www.bioz.com/result/exonuclease i e coli/product/New England Biolabs
Average 99 stars, based on 21 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/exonuclease i e coli/product/New England Biolabs
Average 99 stars, based on 21 article reviews
Price from $9.99 to $1999.99
exonuclease i e coli - by Bioz Stars,
2019-12
99/100 stars
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Clone Assay:Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: Paragraph title: Cloning and sequencing ... Proper-sized transformants were purified with Amplification:Article Title: Natural Genetic Variation of Integrin Alpha L (Itgal) Modulates Ischemic Brain Injury in Stroke Article Snippet: PCR was performed using an appropriate dilution of cDNA generated from the cerebral cortex of F1 (B6×BALB) animals. .. Amplicons containing coding SNPs were amplified by conventional PCR, and 15 ul of PCR products were treated with 1 U Article Title: Y-chromosome evidence supports asymmetric dog introgression into eastern coyotes Article Snippet: Paragraph title: Mitochondrial DNA control region and Zfy intron amplification and sequencing ... We purified polymerase chain reaction (PCR) products using Exosap-IT (USB Corporation, Cleveland, OH), or Article Title: Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations Article Snippet: Amplicon lengths ranged from 120 to 190 bp in size depending on the amplicon ( Supplementary Table S2 ). .. Following multiplex cycling, 1 µl of Article Title: Magnetic resonance imaging and genetic investigation of a case of rottweiler leukoencephalomyelopathy Article Snippet: For the DARS2 mutation analysis, suitable PCR products were amplified using AmpliTaq Gold 360 (Life Technologies). .. The PCR products were resequenced after rAPid alkaline phosphatase (Roche) and Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: Primers 8817–8878 at 300 nM concentration were used in amplification of the genes 1–31 with 35 cycles. .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Article Title: Evaluation of high throughput gene expression platforms using a genomic biomarker signature for prediction of skin sensitization Article Snippet: Specific Target Amplification (STA) was performed on cDNA samples using TaqMan® PreAmp Master Mix (Life Technologies™) and standard protocols provided by manufacturer. .. Samples were treated with Article Title: A recombineering based approach for high-throughput conditional knockout targeting vector construction Article Snippet: PCR amplification was carried out using Extensor Hi-Fidelity PCR Master Mix 2 (2X, ABgene). .. This was then followed by 68°C for 5 min. After PCR reactions, 0.5 μl of Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: The reaction conditions were as follows: 95° for 2 min, followed by 35 cycles of 95° for 30 s, annealing temperature (Table ) for 30 s, and 72° for 30 s, with final extension of 72° for 5 min. Amplification was performed in an MJ Research Tetrad Thermal Cycler (MJ Research, Waltham, MA). .. If sequencing was needed, PCR products were first treated with Article Title: Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1 Article Snippet: Genomic DNA was amplified by yeast colony polymerase chain reaction (PCR), using a blend of Pwo and Taq polymerase. .. The resulting 2 kb PCR products were treated with Article Title: A locus for an auditory processing deficit and language impairment in an extended pedigree maps to 12p13.31-q14.3 Article Snippet: Products were amplified using a ‘touch-down’ PCR programme, decreasing the annealing temperature from 67.5 to 61°C over 13 cycles, with a further 29 cycles at 61°C. .. PCR product clean-up was carried out using Filtration:Article Title: A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Article Snippet: DNA was precipitated with EtOH, washed with 70% EtOH and resuspended in 100 µl of water. .. To select the crosslinked fragments, DNA samples were boiled for 2 min, chilled on ice and then digested with excess Positive Control:Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: One three-spined stickleback individual from the Baltic Sea (60°12' N, 25°11' E) was used as a positive control. .. PCR products were purified using Synthesized:Article Title: Genetic variants of FZD4 and LRP5 genes in patients with advanced retinopathy of prematurity Article Snippet: To identify variants in all coding exons of the FZD4 , LRP5, TSPAN12, and NDP genes, oligonucleotide primers on the flanking intron/untranslated region (UTR) sequences were designed and synthesized as described [ - ]. .. The PCR products were treated with shrimp alkaline phosphatase (Roche Applied Science, Indianapolis, IN) and Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: Synthesized universal fragments were gel-purified (QIAquick Gel Extraction Kit, Qiagen). .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml TA Cloning:Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: PCR products from rhizosphere soils were cloned using INVα F' competent cells and the pCR®2.1 vector from the TA Cloning® Kit (Life Technologies, Grand Island, NY) according to the manufacturer's directions. .. Proper-sized transformants were purified with Construct:Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: The “pooled” gene assay mix was constructed by combining 1μl of all 96 primers (forward and reverse combined) with DNA suspension buffer (104 μl) (Teknova #T0223). .. Following pre-amplification unincorporated primers were removed by Gene Assay:Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: The “pooled” gene assay mix was constructed by combining 1μl of all 96 primers (forward and reverse combined) with DNA suspension buffer (104 μl) (Teknova #T0223). .. Following pre-amplification unincorporated primers were removed by Incubation:Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: The “pooled” gene assay mix was constructed by combining 1μl of all 96 primers (forward and reverse combined) with DNA suspension buffer (104 μl) (Teknova #T0223). .. Following pre-amplification unincorporated primers were removed by Article Title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging Article Snippet: This step not only makes the eluted DNA double stranded to be precisely size-selected by SPRI beads, but also synthesizes the sequence required for bridge PCR. .. We transferred the supernatant to a new tube, added 1 μl of 20 U/μl Article Title: Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations Article Snippet: Amplicon lengths ranged from 120 to 190 bp in size depending on the amplicon ( Supplementary Table S2 ). .. Following multiplex cycling, 1 µl of Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: Transformants were transferred to Luria-Bertani (LB) broth containing kanamycin (50 μ g/mL) in 96-well tissue culture plates, incubated for 16 h at 37°C at 225 rpm, then screened with PCR using the M13f and M13r primers ( ) to check for the presence of an insert. .. Proper-sized transformants were purified with Article Title: A recombineering based approach for high-throughput conditional knockout targeting vector construction Article Snippet: PCR was performed using PTC-225 PCR machine (Peltier Thermal Cycler) with the following settings: 94°C for 4 min, this was followed by 35 cycles of 94°C for 30 s, 60°C for 30 s and 68°C for 1 min (Bsd ) or 2–3 min (Neo and retrieval backbone). .. This was then followed by 68°C for 5 min. After PCR reactions, 0.5 μl of Article Title: A molecular inversion probe assay for detecting alternative splicing Article Snippet: The wash buffer was removed from the beads and 10 μl of ligation mix (1 μl Ampligase, 1 μl 10× ligation buffer and 8 μl water) was added; the ligation reaction was incubated at 58°C for 30 minutes. .. The ligated MIPs were further purified and fully eluted from the immobilized cDNA by digesting all linear DNA with exonucleases; 4 μl of Article Title: A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Article Snippet: Following an overnight incubation at 37°C with proteinase K and RNAse I, the lysates were extracted twice with phenol and once with phenol–chloroform. .. To select the crosslinked fragments, DNA samples were boiled for 2 min, chilled on ice and then digested with excess Article Title: Mechanistic insights into the role of Hop2-Mnd1 in meiotic homologous DNA pairing Article Snippet: Protection of ssDNA against the action of E. coli Exo I by DMC1 with and without Hop2–Mnd1 was conducted as described ( ). .. Presynaptic filaments were assembled with DMC1 (0.3 µM) and 5' 32 P-labeled 100-mer ssDNA (3 µM nucleotides) and then incubated with Article Title: Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis Article Snippet: One-cell stage embryos were microinjected with 1–2 nl of the mixture, and cultured until shield to 80% epiboly stages. .. To extract genomic DNA, individual embryos were placed into 25 μl of ice-cold DNA extraction buffer (10 mM Tris–HCl, pH 8.0, 2 mM EDTA, 0.2% Triton X-100, 200 μg/ml Proteinase K) and incubated at 50°C for 16 h, then at 95°C for 10 min. To remove remaining targeting oligonucleotide, 25 μl of Expressing:Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease Article Snippet: Paragraph title: Single-cell gene expression analysis ... Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Article Title: Natural Genetic Variation of Integrin Alpha L (Itgal) Modulates Ischemic Brain Injury in Stroke Article Snippet: Paragraph title: Allele-specific SNapShot gene expression analysis ... Amplicons containing coding SNPs were amplified by conventional PCR, and 15 ul of PCR products were treated with 1 U Article Title: Evaluation of high throughput gene expression platforms using a genomic biomarker signature for prediction of skin sensitization Article Snippet: Paragraph title: Measurement of gene expression on BioMark™ HD system ... Samples were treated with Modification:Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: The genomic PCR was performed similarly to the plasmid-programmed reaction with the modification in the annealing step (temperature decrement of 1° from 68°C to 60°C on the first eight cycles was used, then 27 cycles at 60°C, annealing for 50 seconds) and prolonged to 40 seconds elongation step. .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Ligation:Article Title: A molecular inversion probe assay for detecting alternative splicing Article Snippet: Following ligation, the beads were washed three times with Dynabead wash buffer: twice at 58°C and once at room temperature. .. The ligated MIPs were further purified and fully eluted from the immobilized cDNA by digesting all linear DNA with exonucleases; 4 μl of Generated:Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease Article Snippet: Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Article Title: Natural Genetic Variation of Integrin Alpha L (Itgal) Modulates Ischemic Brain Injury in Stroke Article Snippet: PCR was performed using an appropriate dilution of cDNA generated from the cerebral cortex of F1 (B6×BALB) animals. .. Amplicons containing coding SNPs were amplified by conventional PCR, and 15 ul of PCR products were treated with 1 U Cell Culture:Article Title: Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis Article Snippet: One-cell stage embryos were microinjected with 1–2 nl of the mixture, and cultured until shield to 80% epiboly stages. .. To extract genomic DNA, individual embryos were placed into 25 μl of ice-cold DNA extraction buffer (10 mM Tris–HCl, pH 8.0, 2 mM EDTA, 0.2% Triton X-100, 200 μg/ml Proteinase K) and incubated at 50°C for 16 h, then at 95°C for 10 min. To remove remaining targeting oligonucleotide, 25 μl of Bridge PCR:Article Title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging Article Snippet: This step not only makes the eluted DNA double stranded to be precisely size-selected by SPRI beads, but also synthesizes the sequence required for bridge PCR. .. We transferred the supernatant to a new tube, added 1 μl of 20 U/μl Overlap Extension Polymerase Chain Reaction:Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: Paragraph title: Preparation of PCR products for OE-PCR ... Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml DNA Sequencing:Article Title: Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1 Article Snippet: Paragraph title: Genomic DNA sequencing ... The resulting 2 kb PCR products were treated with Sequencing:Article Title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging Article Snippet: This step not only makes the eluted DNA double stranded to be precisely size-selected by SPRI beads, but also synthesizes the sequence required for bridge PCR. .. We transferred the supernatant to a new tube, added 1 μl of 20 U/μl Article Title: Y-chromosome evidence supports asymmetric dog introgression into eastern coyotes Article Snippet: For a subsample of males we amplified a 658 bp fragment of the last intron of the Zfy gene with published primers (LGL-331: Shaw et al. ; Yint-2-335: Wilson et al. ) as in Wilson et al. ( ). .. We purified polymerase chain reaction (PCR) products using Exosap-IT (USB Corporation, Cleveland, OH), or Article Title: Genetic variants of FZD4 and LRP5 genes in patients with advanced retinopathy of prematurity Article Snippet: The PCR products were treated with shrimp alkaline phosphatase (Roche Applied Science, Indianapolis, IN) and Article Title: Magnetic resonance imaging and genetic investigation of a case of rottweiler leukoencephalomyelopathy Article Snippet: For the DARS2 mutation analysis, suitable PCR products were amplified using AmpliTaq Gold 360 (Life Technologies). .. The PCR products were resequenced after rAPid alkaline phosphatase (Roche) and Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: Transformants were transferred to Luria-Bertani (LB) broth containing kanamycin (50 μ g/mL) in 96-well tissue culture plates, incubated for 16 h at 37°C at 225 rpm, then screened with PCR using the M13f and M13r primers ( ) to check for the presence of an insert. .. Proper-sized transformants were purified with Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: Alternatively, the PCR products were separated using an HDA-GT12 DNA analyzer and scored by Biocalculator software (both from eGene, Irvine, CA). .. If sequencing was needed, PCR products were first treated with Article Title: Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1 Article Snippet: Genomic DNA was amplified by yeast colony polymerase chain reaction (PCR), using a blend of Pwo and Taq polymerase. .. The resulting 2 kb PCR products were treated with Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: PCR products were purified using Article Title: A locus for an auditory processing deficit and language impairment in an extended pedigree maps to 12p13.31-q14.3 Article Snippet: All primer sequences can be found in Supporting Information, . .. PCR product clean-up was carried out using Injection:Article Title: Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis Article Snippet: Paragraph title: Microinjection of targeting oligonucleotides/Rad52 mixtures and DNA extraction from injected embryos ... To extract genomic DNA, individual embryos were placed into 25 μl of ice-cold DNA extraction buffer (10 mM Tris–HCl, pH 8.0, 2 mM EDTA, 0.2% Triton X-100, 200 μg/ml Proteinase K) and incubated at 50°C for 16 h, then at 95°C for 10 min. To remove remaining targeting oligonucleotide, 25 μl of Binding Assay:Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease Article Snippet: Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: Following pre-amplification unincorporated primers were removed by Article Title: Evaluation of high throughput gene expression platforms using a genomic biomarker signature for prediction of skin sensitization Article Snippet: Samples were treated with Staining:Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: Using the same staining protocol and gating strategy outlined above, 10 cells in triplicate were sorted from selected cTfh subsets of 5 normal subjects and 4 MZL patients. .. Following pre-amplification unincorporated primers were removed by Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: PCR samples were stained prior to electrophoresis with 1× GelRed (Biotium, Hayward, CA). .. If sequencing was needed, PCR products were first treated with DNA Extraction:Article Title: Genetic variants of FZD4 and LRP5 genes in patients with advanced retinopathy of prematurity Article Snippet: DNA samples were extracted from peripheral blood using a DNA extraction kit (QiaAmp; Qiagen, Chatsworth, CA). .. The PCR products were treated with shrimp alkaline phosphatase (Roche Applied Science, Indianapolis, IN) and Article Title: Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis Article Snippet: One-cell stage embryos were microinjected with 1–2 nl of the mixture, and cultured until shield to 80% epiboly stages. .. To extract genomic DNA, individual embryos were placed into 25 μl of ice-cold DNA extraction buffer (10 mM Tris–HCl, pH 8.0, 2 mM EDTA, 0.2% Triton X-100, 200 μg/ml Proteinase K) and incubated at 50°C for 16 h, then at 95°C for 10 min. To remove remaining targeting oligonucleotide, 25 μl of In Vivo:Article Title: A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Article Snippet: Paragraph title: In vivo TMP–DNA PB and selection of DNA-crosslinked fragments ... To select the crosslinked fragments, DNA samples were boiled for 2 min, chilled on ice and then digested with excess Mutagenesis:Article Title: Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations Article Snippet: These regions contain a mutant count of greater than 1 for any single point mutation. .. Following multiplex cycling, 1 µl of Article Title: Magnetic resonance imaging and genetic investigation of a case of rottweiler leukoencephalomyelopathy Article Snippet: For the DARS2 mutation analysis, suitable PCR products were amplified using AmpliTaq Gold 360 (Life Technologies). .. The PCR products were resequenced after rAPid alkaline phosphatase (Roche) and Isolation:Article Title: A locus for an auditory processing deficit and language impairment in an extended pedigree maps to 12p13.31-q14.3 Article Snippet: Sequencing was performed on DNA samples isolated from three of the most severely affected family members (II-3, III-4 and III-6). .. PCR product clean-up was carried out using Size-exclusion Chromatography:Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease Article Snippet: Next, pre-amplification mix, consisting of pooled mixture of all primer assays (500nM), 5× PreAmp Master Mix (Fluidigm) and H2 O, was added to each well and run on a thermocycler (95°C for 5 min followed by 18 cycles: 96°C for 5 sec 60°C for 6 min). .. Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Labeling:Article Title: Natural Genetic Variation of Integrin Alpha L (Itgal) Modulates Ischemic Brain Injury in Stroke Article Snippet: Amplicons containing coding SNPs were amplified by conventional PCR, and 15 ul of PCR products were treated with 1 U Purification:Article Title: Y-chromosome evidence supports asymmetric dog introgression into eastern coyotes Article Snippet: For a subsample of males we amplified a 658 bp fragment of the last intron of the Zfy gene with published primers (LGL-331: Shaw et al. ; Yint-2-335: Wilson et al. ) as in Wilson et al. ( ). .. We purified polymerase chain reaction (PCR) products using Exosap-IT (USB Corporation, Cleveland, OH), or Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: Transformants were transferred to Luria-Bertani (LB) broth containing kanamycin (50 μ g/mL) in 96-well tissue culture plates, incubated for 16 h at 37°C at 225 rpm, then screened with PCR using the M13f and M13r primers ( ) to check for the presence of an insert. .. Proper-sized transformants were purified with Article Title: A recombineering based approach for high-throughput conditional knockout targeting vector construction Article Snippet: This was then followed by 68°C for 5 min. After PCR reactions, 0.5 μl of exonuclease I (10 U, from either New England Biolabs or Epicentre) was added per 50 μl of PCR products and incubated at 37°C for 1 h followed by heat inactivation at 80°C for 20 min. .. This was then followed by 68°C for 5 min. After PCR reactions, 0.5 μl of Article Title: A molecular inversion probe assay for detecting alternative splicing Article Snippet: Circularized MIPs were dissociated from the cDNA by holding the sample at 95°C for 5 minutes and chilled on ice. .. The ligated MIPs were further purified and fully eluted from the immobilized cDNA by digesting all linear DNA with exonucleases; 4 μl of Article Title: A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Article Snippet: DNA was precipitated with EtOH, washed with 70% EtOH and resuspended in 100 µl of water. .. To select the crosslinked fragments, DNA samples were boiled for 2 min, chilled on ice and then digested with excess Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: Approximate size of the PCR amplicons was determined by electrophoresis on 1.5% agarose gel with a DNA ladder (GeneRuler™ DNA Ladder Mix, Fermentas). .. PCR products were purified using Polymerase Chain Reaction:Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease Article Snippet: Similar to before [ ], PCR plates were thawed and pre-heated for 90 seconds at 65°C. .. Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Article Title: Natural Genetic Variation of Integrin Alpha L (Itgal) Modulates Ischemic Brain Injury in Stroke Article Snippet: PCR was performed using an appropriate dilution of cDNA generated from the cerebral cortex of F1 (B6×BALB) animals. .. Amplicons containing coding SNPs were amplified by conventional PCR, and 15 ul of PCR products were treated with 1 U Article Title: Y-chromosome evidence supports asymmetric dog introgression into eastern coyotes Article Snippet: For a subsample of males we amplified a 658 bp fragment of the last intron of the Zfy gene with published primers (LGL-331: Shaw et al. ; Yint-2-335: Wilson et al. ) as in Wilson et al. ( ). .. We purified polymerase chain reaction (PCR) products using Exosap-IT (USB Corporation, Cleveland, OH), or Article Title: Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations Article Snippet: Multiplex PCR cycling was performed according to the manufacturer’s recommendations (Kapa Biosystems) for a total of 15–25 PCR cycles using 63°C as optimal annealing temperature. .. Following multiplex cycling, 1 µl of Article Title: Genetic variants of FZD4 and LRP5 genes in patients with advanced retinopathy of prematurity Article Snippet: Polymerase chain reaction (PCR) was performed with optimized annealing temperatures. .. The PCR products were treated with shrimp alkaline phosphatase (Roche Applied Science, Indianapolis, IN) and Article Title: Magnetic resonance imaging and genetic investigation of a case of rottweiler leukoencephalomyelopathy Article Snippet: For the DARS2 mutation analysis, suitable PCR products were amplified using AmpliTaq Gold 360 (Life Technologies). .. The PCR products were resequenced after rAPid alkaline phosphatase (Roche) and Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: Transformants were transferred to Luria-Bertani (LB) broth containing kanamycin (50 μ g/mL) in 96-well tissue culture plates, incubated for 16 h at 37°C at 225 rpm, then screened with PCR using the M13f and M13r primers ( ) to check for the presence of an insert. .. Proper-sized transformants were purified with Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: Synthesized universal fragments were gel-purified (QIAquick Gel Extraction Kit, Qiagen). .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Article Title: Evaluation of high throughput gene expression platforms using a genomic biomarker signature for prediction of skin sensitization Article Snippet: The PCR plate was transferred to a thermal cycler and subjected to the following thermal protocol: 95°C, 10 min; 14 cycles (95°C ,15 s; 60°C, 4 min); 4°C hold. .. Samples were treated with Article Title: A recombineering based approach for high-throughput conditional knockout targeting vector construction Article Snippet: PCR was performed using PTC-225 PCR machine (Peltier Thermal Cycler) with the following settings: 94°C for 4 min, this was followed by 35 cycles of 94°C for 30 s, 60°C for 30 s and 68°C for 1 min (Bsd ) or 2–3 min (Neo and retrieval backbone). .. This was then followed by 68°C for 5 min. After PCR reactions, 0.5 μl of Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: Alternatively, the PCR products were separated using an HDA-GT12 DNA analyzer and scored by Biocalculator software (both from eGene, Irvine, CA). .. If sequencing was needed, PCR products were first treated with Article Title: Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1 Article Snippet: Genomic DNA was amplified by yeast colony polymerase chain reaction (PCR), using a blend of Pwo and Taq polymerase. .. The resulting 2 kb PCR products were treated with Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: Approximate size of the PCR amplicons was determined by electrophoresis on 1.5% agarose gel with a DNA ladder (GeneRuler™ DNA Ladder Mix, Fermentas). .. PCR products were purified using Article Title: A locus for an auditory processing deficit and language impairment in an extended pedigree maps to 12p13.31-q14.3 Article Snippet: All primer sequences can be found in Supporting Information, . .. PCR product clean-up was carried out using Quantitative RT-PCR:Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: Paragraph title: Microfluidic RT-qPCR ... Following pre-amplification unincorporated primers were removed by FACS:Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease Article Snippet: After FACS sorting, PCR plates were frozen and kept in -80°C until usage. .. Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Activated Clotting Time Assay:Article Title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging Article Snippet: We suspended the collected beads in 50 μl of 1× Phusion HF buffer containing 0.25 mM dNTPs, 2 U Phusion Hot Start High-Fidelity DNA Polymerase (Finnzyme) and 0.8 μM Primer-3 (5′-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC T-3′). .. We transferred the supernatant to a new tube, added 1 μl of 20 U/μl Helicase-dependent Amplification:Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: Alternatively, the PCR products were separated using an HDA-GT12 DNA analyzer and scored by Biocalculator software (both from eGene, Irvine, CA). .. If sequencing was needed, PCR products were first treated with Plasmid Preparation:Article Title: Soil pathogen communities associated with native and non-native Phragmites australis populations in freshwater wetlands Article Snippet: PCR products from rhizosphere soils were cloned using INVα F' competent cells and the pCR®2.1 vector from the TA Cloning® Kit (Life Technologies, Grand Island, NY) according to the manufacturer's directions. .. Proper-sized transformants were purified with Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: In addition, the fragment encoding for PP1 phosphatase catalytic subunit (GeneBank ID NP_002699) was amplified from PP1-encoding plasmid with primers 9176 and 9177 using PCR conditions as given for genomic PCR. .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Software:Article Title: Natural Genetic Variation of Integrin Alpha L (Itgal) Modulates Ischemic Brain Injury in Stroke Article Snippet: Amplicons containing coding SNPs were amplified by conventional PCR, and 15 ul of PCR products were treated with 1 U Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: Alternatively, the PCR products were separated using an HDA-GT12 DNA analyzer and scored by Biocalculator software (both from eGene, Irvine, CA). .. If sequencing was needed, PCR products were first treated with Irradiation:Article Title: A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Article Snippet: To select the crosslinked fragments, DNA samples were boiled for 2 min, chilled on ice and then digested with excess Multiplex Assay:Article Title: Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations Article Snippet: Amplicon lengths ranged from 120 to 190 bp in size depending on the amplicon ( Supplementary Table S2 ). .. Following multiplex cycling, 1 µl of Selection:Article Title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging Article Snippet: We transferred the supernatant to a new tube, added 1 μl of 20 U/μl Article Title: A recombineering based approach for high-throughput conditional knockout targeting vector construction Article Snippet: The restriction maps of the selection cassettes and PL611 are depicted in Supplementary Figures 2–6. .. This was then followed by 68°C for 5 min. After PCR reactions, 0.5 μl of Article Title: A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Article Snippet: Paragraph title: In vivo TMP–DNA PB and selection of DNA-crosslinked fragments ... To select the crosslinked fragments, DNA samples were boiled for 2 min, chilled on ice and then digested with excess Agarose Gel Electrophoresis:Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: Approximate size of the PCR amplicons was determined by electrophoresis on 1.5% agarose gel with a DNA ladder (GeneRuler™ DNA Ladder Mix, Fermentas). .. PCR products were purified using Electrophoresis:Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: PCR samples were stained prior to electrophoresis with 1× GelRed (Biotium, Hayward, CA). .. If sequencing was needed, PCR products were first treated with Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: Approximate size of the PCR amplicons was determined by electrophoresis on 1.5% agarose gel with a DNA ladder (GeneRuler™ DNA Ladder Mix, Fermentas). .. PCR products were purified using Ethanol Precipitation:Article Title: Utility of sequenced genomes for microsatellite marker development in non-model organisms: a case study of functionally important genes in nine-spined sticklebacks (Pungitius pungitius) Article Snippet: PCR products were purified using Concentration Assay:Article Title: Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations Article Snippet: Multiplex pre-amplification from ∼20 ng genomic DNA or 10 ng plasma-circulating DNA was performed in a total volume of 25 µl using a mixture of 100 primers (50 paired sets) at a final concentration of 0.3 µM for each primer with 0.3 mM dNTPs, 3 mM MgCl2 , 1× Kapa HiFi buffer and 0.5 U of Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Woburn, MA, USA) reported to have an error rate of 2.8 × 10−7 mis-incorporations/bp. .. Following multiplex cycling, 1 µl of Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: Primers 8817–8878 at 300 nM concentration were used in amplification of the genes 1–31 with 35 cycles. .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Article Title: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1 Article Snippet: The polymerase chain reaction (PCR) was performed in a 20 μl volume containing 10 ng of genomic DNA as a template, 200 μmol/L of each dNTP, 1× Standard Taq PCR buffer with 1.5 mmol/L MgCl2 , 1.2 U Taq polymerase (all from New England Biolabs, Ipswich, MA), and forward and reverse primers at a concentration of 0.25 μmol/L each. .. If sequencing was needed, PCR products were first treated with Fractionation:Article Title: Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging Article Snippet: Paragraph title: Elution and size fractionation ... We transferred the supernatant to a new tube, added 1 μl of 20 U/μl Migration:Article Title: Circulating Tfh1 (cTfh1) cell numbers and PD1 expression are elevated in low-grade B-cell non-Hodgkin’s lymphoma and cTfh gene expression is perturbed in marginal zone lymphoma Article Snippet: A custom panel of 96 genes (including 4 candidate reference genes) implicated in CD4+ T-cell biology (migration, differentiation, effector function and cell signalling pathways) was created, and primers designed using the D3 Assay Design service (Fluidigm, ) ( ). .. Following pre-amplification unincorporated primers were removed by Gel Extraction:Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System Article Snippet: Synthesized universal fragments were gel-purified (QIAquick Gel Extraction Kit, Qiagen). .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Variant Assay:Article Title: Genetic variants of FZD4 and LRP5 genes in patients with advanced retinopathy of prematurity Article Snippet: The PCR products were treated with shrimp alkaline phosphatase (Roche Applied Science, Indianapolis, IN) and |
