exonuclease digestion  (TaKaRa)


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    TaKaRa exonuclease digestion
    Exonuclease Digestion, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease digestion/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2021-07
    86/100 stars

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    Article Title: The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis
    Article Snippet: Exonuclease digestion of U. maydis DNA The assay was performed with Bal 31 exonuclease (Takara Bio, Shiga, Japan) according to the manufacturer's instructions.

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    TaKaRa bal 31 nuclease
    <t>BAL-31</t> nuclease treatment assay. Genomic DNA (5 μg) from WT at 1,008 PDL, DKO301 at 663 PDL, or DKO741 at 638 PDL was digested with BAL-31 nuclease for 0, 10, or 20 min. Then, DNA was further digested with Hap II and used for TRF analysis. (T 2 AG 3 ) 3 was used as the probe.
    Bal 31 Nuclease, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bal 31 nuclease/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bal 31 nuclease - by Bioz Stars, 2021-07
    86/100 stars
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    95
    TaKaRa exonuclease iii
    Zscan4 leads to longer telomeres in the resultant iPS cells and significantly improves the developmental potential of iPS cells. (A) TRF analysis of iPS cells and control ES cells (E14) between passages 7-10 demonstrated longer telomeres in OSKMZ-induced iPS cells compared to OSKM-induced iPS cells. (B) A histogram shows the distribution of relative telomere length (in TFUs) measured by Q-FISH in iPS cells. The median telomere length (white bars) is shown as the mean ± s.d. above each panel. The passage number was indicated. (C) <t>Three</t> live-born (E19.5) all-iPS cell mice generated from OSKMZ-1 cells via TCA. (D) An adult all-iPS cell mouse generated from OSKMZ-1 via TCA. (E) Genotype analyses of all-iPS cell mice. Retrovirus integration (left) and SSLP (right) analyses were performed using genomic <t>DNA</t> isolated from tail tips of adult mice. Genomic DNA from C57BL/6, DBA and ICR mice served as controls.
    Exonuclease Iii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease iii - by Bioz Stars, 2021-07
    95/100 stars
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    BAL-31 nuclease treatment assay. Genomic DNA (5 μg) from WT at 1,008 PDL, DKO301 at 663 PDL, or DKO741 at 638 PDL was digested with BAL-31 nuclease for 0, 10, or 20 min. Then, DNA was further digested with Hap II and used for TRF analysis. (T 2 AG 3 ) 3 was used as the probe.

    Journal: Molecular and Cellular Biology

    Article Title: Telomere Maintenance in Telomerase-Deficient Mouse Embryonic Stem Cells: Characterization of an Amplified Telomeric DNA

    doi:

    Figure Lengend Snippet: BAL-31 nuclease treatment assay. Genomic DNA (5 μg) from WT at 1,008 PDL, DKO301 at 663 PDL, or DKO741 at 638 PDL was digested with BAL-31 nuclease for 0, 10, or 20 min. Then, DNA was further digested with Hap II and used for TRF analysis. (T 2 AG 3 ) 3 was used as the probe.

    Article Snippet: To further investigate the terminal nature of the 1.6-kb DNA unit, DNA from DKO741 survivor cells at 638 PDL and control cells (WT and DKO301 survivors at 663 PDL) was digested with BAL-31 nuclease for increasing lengths of time.

    Techniques:

    Zscan4 leads to longer telomeres in the resultant iPS cells and significantly improves the developmental potential of iPS cells. (A) TRF analysis of iPS cells and control ES cells (E14) between passages 7-10 demonstrated longer telomeres in OSKMZ-induced iPS cells compared to OSKM-induced iPS cells. (B) A histogram shows the distribution of relative telomere length (in TFUs) measured by Q-FISH in iPS cells. The median telomere length (white bars) is shown as the mean ± s.d. above each panel. The passage number was indicated. (C) Three live-born (E19.5) all-iPS cell mice generated from OSKMZ-1 cells via TCA. (D) An adult all-iPS cell mouse generated from OSKMZ-1 via TCA. (E) Genotype analyses of all-iPS cell mice. Retrovirus integration (left) and SSLP (right) analyses were performed using genomic DNA isolated from tail tips of adult mice. Genomic DNA from C57BL/6, DBA and ICR mice served as controls.

    Journal: Cell Research

    Article Title: Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation

    doi: 10.1038/cr.2012.157

    Figure Lengend Snippet: Zscan4 leads to longer telomeres in the resultant iPS cells and significantly improves the developmental potential of iPS cells. (A) TRF analysis of iPS cells and control ES cells (E14) between passages 7-10 demonstrated longer telomeres in OSKMZ-induced iPS cells compared to OSKM-induced iPS cells. (B) A histogram shows the distribution of relative telomere length (in TFUs) measured by Q-FISH in iPS cells. The median telomere length (white bars) is shown as the mean ± s.d. above each panel. The passage number was indicated. (C) Three live-born (E19.5) all-iPS cell mice generated from OSKMZ-1 cells via TCA. (D) An adult all-iPS cell mouse generated from OSKMZ-1 via TCA. (E) Genotype analyses of all-iPS cell mice. Retrovirus integration (left) and SSLP (right) analyses were performed using genomic DNA isolated from tail tips of adult mice. Genomic DNA from C57BL/6, DBA and ICR mice served as controls.

    Article Snippet: The BrdU-substituted DNA was digested with 3 U/μl Exonuclease III (Takara) for 10 min at room temperature.

    Techniques: Fluorescence In Situ Hybridization, Mouse Assay, Generated, Isolation

    The distal region of the IL-6 promoter contains functional C/EBP motifs A. IL-6 promoter Luciferase activity in PC3 cells transfected with the pGL2-IL-6 deletion mutants shown on the left and pRV with and without treatment with rGal-3BP (2.5 µg/ml) for 24 hours. The data represent the mean (±SD) ratio Firefly Luciferase/Renilla Luciferase and are representative of one among three separate experiments each performed in triplicate samples. B. Sequencing analysis of the region of the IL-6 promoter extending from position −1586 to −1255 showing the presence of 3 C/EBP domains. C. EMSA of nuclear extracts from PC3 cells treated with rGAL-3BP (5 µg/ml) using biotinylated oligonucleotides in the presence of 10× fold excess of non-biotinylated oligonucleotides when indicated.

    Journal: Cancer research

    Article Title: A GALECTIN-3-DEPENDENT PATHWAY UPREGULATES INTERLEUKIN-6 IN THE MICROENVIRONMENT OF HUMAN NEUROBLASTOMA

    doi: 10.1158/0008-5472.CAN-11-2165

    Figure Lengend Snippet: The distal region of the IL-6 promoter contains functional C/EBP motifs A. IL-6 promoter Luciferase activity in PC3 cells transfected with the pGL2-IL-6 deletion mutants shown on the left and pRV with and without treatment with rGal-3BP (2.5 µg/ml) for 24 hours. The data represent the mean (±SD) ratio Firefly Luciferase/Renilla Luciferase and are representative of one among three separate experiments each performed in triplicate samples. B. Sequencing analysis of the region of the IL-6 promoter extending from position −1586 to −1255 showing the presence of 3 C/EBP domains. C. EMSA of nuclear extracts from PC3 cells treated with rGAL-3BP (5 µg/ml) using biotinylated oligonucleotides in the presence of 10× fold excess of non-biotinylated oligonucleotides when indicated.

    Article Snippet: IL-6 promoter deletion mutants in the pGL2-IL-6-Luc construct were generated either by Exonuclease III digestion using the Deletion Kit for kilo sequencing (Takara) in accordance with the instructions of the manufacturer (deletion −1041) or by restriction endonuclease digestion with KpnI and NheI (deletion mutant −212) followed by Klenow fragment reaction at 37°C for 15 minutes.

    Techniques: Functional Assay, Luciferase, Activity Assay, Transfection, Sequencing