exonuclease digestion  (TaKaRa)

 
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    Name:
    Exonuclease I
    Description:
    E coli Exonuclease I is a 3 to 5 exonuclease for single stranded DNA degradation resulting in the production of 5 phosphate mononucleotides from the 3 hydroxyl termini of single stranded DNA This 3 to 5 exonuclease is highly specific for single stranded DNA and does not react with double stranded DNA or RNA Exonuclease I is inactivated by heat treatment at 80°C for 15 minutes
    Catalog Number:
    2650b
    Price:
    None
    Size:
    3 750 Units
    Category:
    Exonuclease I Nucleases Modifying enzymes Cloning
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    Structured Review

    TaKaRa exonuclease digestion
    E coli Exonuclease I is a 3 to 5 exonuclease for single stranded DNA degradation resulting in the production of 5 phosphate mononucleotides from the 3 hydroxyl termini of single stranded DNA This 3 to 5 exonuclease is highly specific for single stranded DNA and does not react with double stranded DNA or RNA Exonuclease I is inactivated by heat treatment at 80°C for 15 minutes
    https://www.bioz.com/result/exonuclease digestion/product/TaKaRa
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer
    Article Snippet: .. The 2.5-kbp genomic sequences in the POLD1 and POLE genes that encompass the regions encoding the proofreading domains of pol delta and epsilon ( ) were amplified by PCR using Taq polymerase with the 3′ exonuclease activity, EX Taq (TaKaRa Bio Inc., Otsu, Japan), and the oligonucleotide primers shown in . .. PCR products were directly used as a template for cycle sequencing reactions using BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA), and the reaction products were loaded onto ABI PRISM 310 Genetic Analyzer (Applied Biosystems).

    Mutagenesis:

    Article Title: A GALECTIN-3-DEPENDENT PATHWAY UPREGULATES INTERLEUKIN-6 IN THE MICROENVIRONMENT OF HUMAN NEUROBLASTOMA
    Article Snippet: .. IL-6 promoter deletion mutants in the pGL2-IL-6-Luc construct were generated either by Exonuclease III digestion using the Deletion Kit for kilo sequencing (Takara) in accordance with the instructions of the manufacturer (deletion −1041) or by restriction endonuclease digestion with KpnI and NheI (deletion mutant −212) followed by Klenow fragment reaction at 37°C for 15 minutes. .. Construct −97 was created by PCR reaction using a forward primer: 5’-CCC GGT ACC CAC CCT CAC CCT CCA AC-3’ and the IL-6 reverse primer described above.

    Genomic Sequencing:

    Article Title: Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer
    Article Snippet: .. The 2.5-kbp genomic sequences in the POLD1 and POLE genes that encompass the regions encoding the proofreading domains of pol delta and epsilon ( ) were amplified by PCR using Taq polymerase with the 3′ exonuclease activity, EX Taq (TaKaRa Bio Inc., Otsu, Japan), and the oligonucleotide primers shown in . .. PCR products were directly used as a template for cycle sequencing reactions using BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA), and the reaction products were loaded onto ABI PRISM 310 Genetic Analyzer (Applied Biosystems).

    Generated:

    Article Title: A GALECTIN-3-DEPENDENT PATHWAY UPREGULATES INTERLEUKIN-6 IN THE MICROENVIRONMENT OF HUMAN NEUROBLASTOMA
    Article Snippet: .. IL-6 promoter deletion mutants in the pGL2-IL-6-Luc construct were generated either by Exonuclease III digestion using the Deletion Kit for kilo sequencing (Takara) in accordance with the instructions of the manufacturer (deletion −1041) or by restriction endonuclease digestion with KpnI and NheI (deletion mutant −212) followed by Klenow fragment reaction at 37°C for 15 minutes. .. Construct −97 was created by PCR reaction using a forward primer: 5’-CCC GGT ACC CAC CCT CAC CCT CCA AC-3’ and the IL-6 reverse primer described above.

    Construct:

    Article Title: A GALECTIN-3-DEPENDENT PATHWAY UPREGULATES INTERLEUKIN-6 IN THE MICROENVIRONMENT OF HUMAN NEUROBLASTOMA
    Article Snippet: .. IL-6 promoter deletion mutants in the pGL2-IL-6-Luc construct were generated either by Exonuclease III digestion using the Deletion Kit for kilo sequencing (Takara) in accordance with the instructions of the manufacturer (deletion −1041) or by restriction endonuclease digestion with KpnI and NheI (deletion mutant −212) followed by Klenow fragment reaction at 37°C for 15 minutes. .. Construct −97 was created by PCR reaction using a forward primer: 5’-CCC GGT ACC CAC CCT CAC CCT CCA AC-3’ and the IL-6 reverse primer described above.

    Sequencing:

    Article Title: A GALECTIN-3-DEPENDENT PATHWAY UPREGULATES INTERLEUKIN-6 IN THE MICROENVIRONMENT OF HUMAN NEUROBLASTOMA
    Article Snippet: .. IL-6 promoter deletion mutants in the pGL2-IL-6-Luc construct were generated either by Exonuclease III digestion using the Deletion Kit for kilo sequencing (Takara) in accordance with the instructions of the manufacturer (deletion −1041) or by restriction endonuclease digestion with KpnI and NheI (deletion mutant −212) followed by Klenow fragment reaction at 37°C for 15 minutes. .. Construct −97 was created by PCR reaction using a forward primer: 5’-CCC GGT ACC CAC CCT CAC CCT CCA AC-3’ and the IL-6 reverse primer described above.

    Incubation:

    Article Title: Establishment of DNA-DNA Interactions by the Cohesin Ring
    Article Snippet: .. The recovered DNA was incubated with E. coli exonuclease I (0.5 U/ μl, TaKaRa Bio) in 10 μl of exoI buffer at 30°C for 15 min. .. The reactions were terminated by addition of 2.5 μl of stop solution (1% SDS, 10 mM EDTA and 4 mg/ml protease K), incubated at 37°C for 20 min, and analyzed by 1% agarose gel electrophoresis.

    Activity Assay:

    Article Title: Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer
    Article Snippet: .. The 2.5-kbp genomic sequences in the POLD1 and POLE genes that encompass the regions encoding the proofreading domains of pol delta and epsilon ( ) were amplified by PCR using Taq polymerase with the 3′ exonuclease activity, EX Taq (TaKaRa Bio Inc., Otsu, Japan), and the oligonucleotide primers shown in . .. PCR products were directly used as a template for cycle sequencing reactions using BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA), and the reaction products were loaded onto ABI PRISM 310 Genetic Analyzer (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer
    Article Snippet: .. The 2.5-kbp genomic sequences in the POLD1 and POLE genes that encompass the regions encoding the proofreading domains of pol delta and epsilon ( ) were amplified by PCR using Taq polymerase with the 3′ exonuclease activity, EX Taq (TaKaRa Bio Inc., Otsu, Japan), and the oligonucleotide primers shown in . .. PCR products were directly used as a template for cycle sequencing reactions using BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA), and the reaction products were loaded onto ABI PRISM 310 Genetic Analyzer (Applied Biosystems).

    Recombinant:

    Article Title: An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA
    Article Snippet: .. Exonuclease I, 10× exonuclease I buffer (670 mM Glycine-KOH, 10 mM Dithiothreitol (DTT), 67 mM MgCl2 , pH 9.5), recombinant RNase inhibitor (RRI), and RNase-free water were obtained from TaKaRa Biotechnology Co., Ltd. (Dalian, China). ..

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  • 96
    TaKaRa exonuclease digestion
    Exonuclease Digestion, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease digestion/product/TaKaRa
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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