exonuclease digestion  (New England Biolabs)


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  • 99
    Name:
    Exonuclease I E coli
    Description:
    Exonuclease I E coli 15 000 units
    Catalog Number:
    m0293l
    Price:
    281
    Size:
    15 000 units
    Category:
    Exonucleases
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    New England Biolabs exonuclease digestion
    Exonuclease I E coli
    Exonuclease I E coli 15 000 units
    https://www.bioz.com/result/exonuclease digestion/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System
    Article Snippet: .. Variable ORF-encoded fragments were freed from residual primers by treatment with 15 U/ml Exonuclease I (NEB), which was added directly to the final PCR reaction mixture, for 30 minutes at 37°C followed by nuclease inactivation at 85°C for 30 minutes. .. Synthesis of translational templates by overlap extension (OE-PCR) PCR To obtain the libraries of DNA templates two universal fragments (1, 2 or 3, 4, ) were combined with one of the 31 variable PCR fragments using OE-PCR.

    Article Title: Rational “Error Elimination” Approach to Evaluating Molecular Barcoded Next-Generation Sequencing Data Identifies Low-Frequency Mutations in Hematologic Malignancies
    Article Snippet: .. After first-stage PCR, 1 μL of exonuclease I (20 U/μL; New England BioLabs) was added to the reactions and incubated at 37°C for 30 minutes. .. After the completion of the first-stage PCR or exonuclease I treatment, PCR products were purified with 1× volume of SPRI beads and eluted in 20 μL of 10 mmol/L Tris-HCl (pH 8.0).

    Staining:

    Article Title: Circular Single-Stranded Synthetic DNA Delivery Vectors for MicroRNA
    Article Snippet: .. In cases where the COLIGO was still contaminated by > 5% of the linear oligonucleotide after elution (as determined by gel staining), an Exonuclease I (NEB) digest was done. ..

    Incubation:

    Article Title: Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood
    Article Snippet: .. After a 5-min incubation at 37°C, a 20 µl extension mix containing 66.8 mM Tris–HCl, 16.8 mM ammonium sulfate, 1 mM dithiothreitol, 33 mM magnesium chloride, 62.5 U/ml T4 DNA Polymerase [or 62.5 U/ml Klenow fragment exo(−), 125 U/ml Klenow fragment, 125 U/ml DNA Polymerase I (Fermentas), 250 U/ml Exonuclease I (New England Biolabs)] was added. ..

    Article Title: Rational “Error Elimination” Approach to Evaluating Molecular Barcoded Next-Generation Sequencing Data Identifies Low-Frequency Mutations in Hematologic Malignancies
    Article Snippet: .. After first-stage PCR, 1 μL of exonuclease I (20 U/μL; New England BioLabs) was added to the reactions and incubated at 37°C for 30 minutes. .. After the completion of the first-stage PCR or exonuclease I treatment, PCR products were purified with 1× volume of SPRI beads and eluted in 20 μL of 10 mmol/L Tris-HCl (pH 8.0).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq
    Article Snippet: .. M0293L) Lambda Exonuclease (New England BioLabs, cat.no. .. M0262L) Plasmid-Safe ATP-dependent DNase (Epicentre, cat.no.

    Avidin-Biotin Assay:

    Article Title: The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair
    Article Snippet: .. To detect the presence of 3′ biotin on 3′ ss-overhangs or resection intermediates, the DNA was pre-incubated with ELB buffer or avidin on ice for 5 min, and then treated with Escherichia coli ExoI (NEB, MA) at 22ºC for 60 min. To analyze the intermediates of the 5′ biotin-avidin DNA, DNA was treated with E. coli ExoI (0.2 u/μl, NEB, MA) or RecJ (0.3 u/μl; NEB, MA) at 22°C for 60 min. To detect the presence of 5′ biotin, DNA was pre-incubated with ELB buffer or avidin on ice for 5 min, and then treated with T7 Exo (0.6 unit/μl; NEB, MA) at 22°C for 60 min. .. Reactions were analyzed by 1% TAE-agarose gel electrophoresis and the gels were first stained with SYBR Gold (Invitrogen, CA, USA) and then dried for exposure to film.

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  • 99
    New England Biolabs exonuclease iii
    HCc3 promotes ligase-mediated DNA concatenation. All images are presented as negative images. ( a ) Concatenation of 125-bp (left panel) and 2.8-kb (right panel) DNA fragments to a higher level in the presence of HCc3 at respective dimer/bp ratios. The linearity of the resulting products was confirmed by <t>Exo</t> <t>III</t> digestion (marked with ‘+/−’ signs). The 125-bp product was resolved on a 8% non-denaturing polyacrylamide gel, and the 2.8-kb product on a 1% TAE agarose gel. ( b ) Variations in intensity of DNA concatenation in the presence of HCc3 at different dimer/bp ratios. The marker bands indicate DNA sizes from 3 to 12 kb at 1-kb increments.
    Exonuclease Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii/product/New England Biolabs
    Average 99 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    exonuclease iii - by Bioz Stars, 2020-07
    99/100 stars
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    HCc3 promotes ligase-mediated DNA concatenation. All images are presented as negative images. ( a ) Concatenation of 125-bp (left panel) and 2.8-kb (right panel) DNA fragments to a higher level in the presence of HCc3 at respective dimer/bp ratios. The linearity of the resulting products was confirmed by Exo III digestion (marked with ‘+/−’ signs). The 125-bp product was resolved on a 8% non-denaturing polyacrylamide gel, and the 2.8-kb product on a 1% TAE agarose gel. ( b ) Variations in intensity of DNA concatenation in the presence of HCc3 at different dimer/bp ratios. The marker bands indicate DNA sizes from 3 to 12 kb at 1-kb increments.

    Journal: Nucleic Acids Research

    Article Title: Concentration-dependent organization of DNA by the dinoflagellate histone-like protein HCc3

    doi: 10.1093/nar/gkm165

    Figure Lengend Snippet: HCc3 promotes ligase-mediated DNA concatenation. All images are presented as negative images. ( a ) Concatenation of 125-bp (left panel) and 2.8-kb (right panel) DNA fragments to a higher level in the presence of HCc3 at respective dimer/bp ratios. The linearity of the resulting products was confirmed by Exo III digestion (marked with ‘+/−’ signs). The 125-bp product was resolved on a 8% non-denaturing polyacrylamide gel, and the 2.8-kb product on a 1% TAE agarose gel. ( b ) Variations in intensity of DNA concatenation in the presence of HCc3 at different dimer/bp ratios. The marker bands indicate DNA sizes from 3 to 12 kb at 1-kb increments.

    Article Snippet: If samples were to be treated with Exonuclease III (Exo III) digestion, an equal volume of 2× digestion mixture (2× NEBuffer 1 and 1 U/μl Exonuclease III; New England Biolab) was added to the reaction and the digestion occurred at 37°C for 1 h, prior to the addition of stop solution.

    Techniques: Agarose Gel Electrophoresis, Marker