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Promega exo iii erase a base system
Exo Iii Erase A Base System, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exo iii erase a base system/product/Promega
Average 92 stars, based on 1 article reviews
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exo iii erase a base system - by Bioz Stars, 2020-09
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Sequencing:

Article Title: Tissue-specific restriction of skeletal muscle troponin C gene expression
Article Snippet: .. For the 5′ deletion series, a deletion construct pHFTnC4700CAT was generated in which the distal 5′-flanking sequence between −6200 and −4700 (EcoR I–Bgl II fragment) was replaced by an adapter element that restored these two sites and generated three new unique sites in the order: EcoR I-Xba I-Kpn I-Sal I-Bgl II. pHFTn C4700CAT DNA was double-digested with restriction endonucleases Bgl II and Kpn I, and one-directional deletion clones were generated using the Exo III Erase-a-Base System (Promega). .. The length of TnC 5′-flanking sequences contained in individual constructs was determined by restriction endonuclease mapping.

Clone Assay:

Article Title: Tissue-specific restriction of skeletal muscle troponin C gene expression
Article Snippet: .. For the 5′ deletion series, a deletion construct pHFTnC4700CAT was generated in which the distal 5′-flanking sequence between −6200 and −4700 (EcoR I–Bgl II fragment) was replaced by an adapter element that restored these two sites and generated three new unique sites in the order: EcoR I-Xba I-Kpn I-Sal I-Bgl II. pHFTn C4700CAT DNA was double-digested with restriction endonucleases Bgl II and Kpn I, and one-directional deletion clones were generated using the Exo III Erase-a-Base System (Promega). .. The length of TnC 5′-flanking sequences contained in individual constructs was determined by restriction endonuclease mapping.

Generated:

Article Title: Tissue-specific restriction of skeletal muscle troponin C gene expression
Article Snippet: .. For the 5′ deletion series, a deletion construct pHFTnC4700CAT was generated in which the distal 5′-flanking sequence between −6200 and −4700 (EcoR I–Bgl II fragment) was replaced by an adapter element that restored these two sites and generated three new unique sites in the order: EcoR I-Xba I-Kpn I-Sal I-Bgl II. pHFTn C4700CAT DNA was double-digested with restriction endonucleases Bgl II and Kpn I, and one-directional deletion clones were generated using the Exo III Erase-a-Base System (Promega). .. The length of TnC 5′-flanking sequences contained in individual constructs was determined by restriction endonuclease mapping.

Construct:

Article Title: Tissue-specific restriction of skeletal muscle troponin C gene expression
Article Snippet: .. For the 5′ deletion series, a deletion construct pHFTnC4700CAT was generated in which the distal 5′-flanking sequence between −6200 and −4700 (EcoR I–Bgl II fragment) was replaced by an adapter element that restored these two sites and generated three new unique sites in the order: EcoR I-Xba I-Kpn I-Sal I-Bgl II. pHFTn C4700CAT DNA was double-digested with restriction endonucleases Bgl II and Kpn I, and one-directional deletion clones were generated using the Exo III Erase-a-Base System (Promega). .. The length of TnC 5′-flanking sequences contained in individual constructs was determined by restriction endonuclease mapping.

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  • 93
    Promega exonuclease iii
    Secondary structure of <t>satRPV</t> RNA monomer. (A) Autoradiograph of 5′-end-labeled transcript of monomeric satRPV RNA after partial digestion with imidazole or RNase T 1 . Gel-purified, end-labeled RNA was incubated in <t>three</t> different molarities of imidazole under nondenaturing (native) conditions for 15 h (lanes 3 to 6). Indicated units of RNase T 1 were used for digestion in nondenaturing (native) conditions for 5 min at 25°C. To generate the G-track sequencing ladder, indicated units of RNase T 1 ) superimposed with markers indicating intensity of cleavages in panel A. Open, filled, and double symbols indicate weak, moderate, and strong cuts, respectively.
    Exonuclease Iii, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii/product/Promega
    Average 93 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    exonuclease iii - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    85
    Promega exonuclease iii based erase a base kit
    Secondary structure of <t>satRPV</t> RNA monomer. (A) Autoradiograph of 5′-end-labeled transcript of monomeric satRPV RNA after partial digestion with imidazole or RNase T 1 . Gel-purified, end-labeled RNA was incubated in <t>three</t> different molarities of imidazole under nondenaturing (native) conditions for 15 h (lanes 3 to 6). Indicated units of RNase T 1 were used for digestion in nondenaturing (native) conditions for 5 min at 25°C. To generate the G-track sequencing ladder, indicated units of RNase T 1 ) superimposed with markers indicating intensity of cleavages in panel A. Open, filled, and double symbols indicate weak, moderate, and strong cuts, respectively.
    Exonuclease Iii Based Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease iii based erase a base kit/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease iii based erase a base kit - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Secondary structure of satRPV RNA monomer. (A) Autoradiograph of 5′-end-labeled transcript of monomeric satRPV RNA after partial digestion with imidazole or RNase T 1 . Gel-purified, end-labeled RNA was incubated in three different molarities of imidazole under nondenaturing (native) conditions for 15 h (lanes 3 to 6). Indicated units of RNase T 1 were used for digestion in nondenaturing (native) conditions for 5 min at 25°C. To generate the G-track sequencing ladder, indicated units of RNase T 1 ) superimposed with markers indicating intensity of cleavages in panel A. Open, filled, and double symbols indicate weak, moderate, and strong cuts, respectively.

    Journal: Journal of Virology

    Article Title: cis and trans Requirements for Rolling Circle Replication of a Satellite RNA

    doi: 10.1128/JVI.78.6.3072-3082.2004

    Figure Lengend Snippet: Secondary structure of satRPV RNA monomer. (A) Autoradiograph of 5′-end-labeled transcript of monomeric satRPV RNA after partial digestion with imidazole or RNase T 1 . Gel-purified, end-labeled RNA was incubated in three different molarities of imidazole under nondenaturing (native) conditions for 15 h (lanes 3 to 6). Indicated units of RNase T 1 were used for digestion in nondenaturing (native) conditions for 5 min at 25°C. To generate the G-track sequencing ladder, indicated units of RNase T 1 ) superimposed with markers indicating intensity of cleavages in panel A. Open, filled, and double symbols indicate weak, moderate, and strong cuts, respectively.

    Article Snippet: Serial deletion mutants (see Fig. ) of satRPV were generated by using exonuclease III (ExoIII; Erase-a-Base system; Promega) after digestion of plasmid pWT with Msc I (construct M122), Ava I (A222, A241, A243) or Cla I (C321).

    Techniques: Autoradiography, Labeling, Purification, Incubation, Sequencing

    Repression of HIF-1β promoter activity by IFN-γ. A , Cloned HIF-1β promoter and series of truncated promoter-luciferase reporter constructs generated using exonuclease III digestion. Relative positions of each clone and major TSSs

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IFN-? Attenuates Hypoxia-Inducible Factor (HIF) Activity in Intestinal Epithelial Cells through Transcriptional Repression of HIF-1?

    doi: 10.4049/jimmunol.1001442

    Figure Lengend Snippet: Repression of HIF-1β promoter activity by IFN-γ. A , Cloned HIF-1β promoter and series of truncated promoter-luciferase reporter constructs generated using exonuclease III digestion. Relative positions of each clone and major TSSs

    Article Snippet: Sequential truncations of the cloned HIF-1β promoter sequence were generated by exonuclease III digestion (Erase-a-Base; Promega).

    Techniques: Activity Assay, Clone Assay, Luciferase, Construct, Generated