ex taq polymerase  (TaKaRa)

 
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    Name:
    TaKaRa Ex Taq DNA Polymerase
    Description:
    TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3 to 5 exonuclease for high sensitivity high efficiency PCR reactions It can also be used for long range PCR up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4 5 times lower as determined by the Kunkel method Ex Taq polymerase is supplied with optimized 10X buffer with or without Mg2 and dNTPs
    Catalog Number:
    rr001c
    Price:
    None
    Size:
    3 000 Units
    Category:
    Ex Taq polymerase Ex Taq products High yield PCR PCR
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    Structured Review

    TaKaRa ex taq polymerase
    Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were <t>TaKaRa</t> Ex <t>Taq</t> ™ and KOD FX Neo ™ .
    TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3 to 5 exonuclease for high sensitivity high efficiency PCR reactions It can also be used for long range PCR up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4 5 times lower as determined by the Kunkel method Ex Taq polymerase is supplied with optimized 10X buffer with or without Mg2 and dNTPs
    https://www.bioz.com/result/ex taq polymerase/product/TaKaRa
    Average 99 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    ex taq polymerase - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

    Journal: Kobe Journal of Medical Sciences

    doi:

    Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .
    Figure Legend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

    Techniques Used: DNA Extraction

    2) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

    Journal: Kobe Journal of Medical Sciences

    doi:

    Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .
    Figure Legend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

    Techniques Used: DNA Extraction

    3) Product Images from "Distinct DNA Methylation Dynamics of Spermatogenic Cell-Specific Intronless Genes Is Associated with CpG Content"

    Article Title: Distinct DNA Methylation Dynamics of Spermatogenic Cell-Specific Intronless Genes Is Associated with CpG Content

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0043658

    Methylation profiles of spermatogenic cell-specific intronless genes are classified three groups in juvenile mouse testis. COBRA was carried out to examine the methylation status of the selected genes, the CpG island in the Oaz1 gene, and constitutively methylated endogenous IAP retroviruses in genomic DNA isolated from D7, D14, D21, and D35 testes and adult liver. Sodium bisulfite-treated DNA was amplified with specific primers (details in Table S3 ) and digested with HpyCH4 IV (recognition site: ACGT), Taq I (TCGA) or Acc II (CGCG).
    Figure Legend Snippet: Methylation profiles of spermatogenic cell-specific intronless genes are classified three groups in juvenile mouse testis. COBRA was carried out to examine the methylation status of the selected genes, the CpG island in the Oaz1 gene, and constitutively methylated endogenous IAP retroviruses in genomic DNA isolated from D7, D14, D21, and D35 testes and adult liver. Sodium bisulfite-treated DNA was amplified with specific primers (details in Table S3 ) and digested with HpyCH4 IV (recognition site: ACGT), Taq I (TCGA) or Acc II (CGCG).

    Techniques Used: Methylation, Combined Bisulfite Restriction Analysis Assay, Isolation, Amplification

    4) Product Images from "Deficiency of a pyrroline-5-carboxylate reductase produces the yellowish green cocoon ‘Ryokuken’ of the silkworm, Bombyx mori"

    Article Title: Deficiency of a pyrroline-5-carboxylate reductase produces the yellowish green cocoon ‘Ryokuken’ of the silkworm, Bombyx mori

    Journal: Heredity

    doi: 10.1038/s41437-018-0051-8

    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
    Figure Legend Snippet: Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region

    Techniques Used: Polymerase Chain Reaction, Marker

    5) Product Images from "Deficiency of a pyrroline-5-carboxylate reductase produces the yellowish green cocoon ‘Ryokuken’ of the silkworm, Bombyx mori"

    Article Title: Deficiency of a pyrroline-5-carboxylate reductase produces the yellowish green cocoon ‘Ryokuken’ of the silkworm, Bombyx mori

    Journal: Heredity

    doi: 10.1038/s41437-018-0051-8

    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
    Figure Legend Snippet: Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region

    Techniques Used: Polymerase Chain Reaction, Marker

    Related Articles

    Polymerase Chain Reaction:

    Article Title: RNA sequencing of the nephron transcriptome: a technical note
    Article Snippet: .. Finally, this cDNA molecule is amplified (the first-round PCR, 18–20 cycles) using the same universal primers (UP1 and UP2) and a high-performance DNA polymerase [TaKaRa Ex Taq HS DNA polymerase (Clontech Laboratories, Mountain View, CA, USA)]. .. Once the first-round PCR is complete, a quantitative real-time PCR (qRT-PCR) for a housekeeping gene (e.g., glutaraldehyde-3-dehydrogenase, beta-actin) is performed to see if the reverse transcription and amplification are successful.

    Article Title: The Protein Phosphatase AtPP2CA Negatively Regulates Abscisic Acid Signal Transduction in Arabidopsis, and Effects of abh1 on AtPP2CA mRNA 1 mRNA 1 [W]
    Article Snippet: .. Reverse transcription (first-strand cDNA synthesis kit, Amersham Biosciences) was performed on 2.5 μ g of RNA and 2 μ L were used for PCR reactions ( Ex Taq DNA polymerase; TaKaRa Mirus Bio). .. Samples were withdrawn after 20, 24, 28, and 32 cycles (splicing) or 28, 32, and 36 cycles (T-DNA disruption lines) and products were analyzed by agarose gel electrophoresis.

    Article Title: Distinct DNA Methylation Dynamics of Spermatogenic Cell-Specific Intronless Genes Is Associated with CpG Content
    Article Snippet: .. Reverse Transcription (RT)-PCR One microgram of total RNA was treated with DNase I at 37°C for 20 min, and the cDNA was reverse-transcribed using SuperScript III (Invitrogen) at 50°C for 60 min. Ex Taq Polymerase (TaKaRa) was then used for PCR with the primers and conditions shown in . .. Combined Bisulfite Restriction Analysis (COBRA) One microgram of genomic DNA was treated with bisulfite using an EZ DNA methylation Kit (Zymo Research), and 50 ng of the bisulfite-treated DNA was used for PCR with the primers and conditions shown in .

    Article Title: Viability of an Escherichia coli pgsA Null Mutant Lacking Detectable Phosphatidylglycerol and Cardiolipin
    Article Snippet: .. DNA from fresh colonies of the strains to be examined was used as a template for PCR amplification with Ex Taq DNA polymerase (Takara, Tokyo, Japan). ..

    Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
    Article Snippet: .. The PCR conditions consisted of 5 µl (50 ng/µl) of DNA and 1 µl each of primer (50 pM) in a 5 µl of 10× reaction buffer with 5 µl of 25 mM MgCl2 , 5 µl of 10 mM dNTP (each 2.5 mM) and 1 µl of 5 U Ex Taq DNA polymerase (TaKaRa, Japan) to a final volume of 50 µl on a thermal cycler (PTC-100; MJ Research, USA). .. PCR was initiated after an incubation step at 94℃ for 3 min, followed by 30 cycles of 94℃ for 30 s, 55℃ for 30 s, and 72℃ for 30 s, with a final extension step at 72℃ for 5 min. PCR products that were 517 bp were excised and purified from agarose gels using a Geneclean II Kit (Qbiogene, USA).

    Article Title: Integrating Morphology, Breeding Ground and Mitochondrial COI Gene Analysis for Species Identification of Bellamya lithophaga (Gastropoda: Viviparidae) in China
    Article Snippet: .. PCR reagents Ex-Taq DNA Polymerase, PCR buffer, MgCl2 and dNTPs (TaKaRa Biotech Co., Ltd, Dalian, China);Protease K (Merck, US-A);WizardTM DNA Clean-up System (Pro-mega, USA). ..

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
    Article Snippet: .. When TaKaRa Ex Taq™ polymerase was used, PCR with DNA preparation methods (A), (B), (C) and (D) generated high amount of PCR products at 40 cycles and the agarose gel electrophoresis showed clear bands of the PCR products. .. However, PCR with DNA preparation method (E) did not generate enough amount of PCR products at 40 cycles and the agarose gel electrophoresis showed no band of the PCR product ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Distinct DNA Methylation Dynamics of Spermatogenic Cell-Specific Intronless Genes Is Associated with CpG Content
    Article Snippet: .. Reverse Transcription (RT)-PCR One microgram of total RNA was treated with DNase I at 37°C for 20 min, and the cDNA was reverse-transcribed using SuperScript III (Invitrogen) at 50°C for 60 min. Ex Taq Polymerase (TaKaRa) was then used for PCR with the primers and conditions shown in . .. Combined Bisulfite Restriction Analysis (COBRA) One microgram of genomic DNA was treated with bisulfite using an EZ DNA methylation Kit (Zymo Research), and 50 ng of the bisulfite-treated DNA was used for PCR with the primers and conditions shown in .

    Generated:

    Article Title: Genomic SELEX Search for Target Promoters under the Control of the PhoQP-RstBA Signal Relay Cascade ▿
    Article Snippet: .. Probes were generated by PCR amplification of the asr , csgD , nikA , yqaD , ptsP , ykfG , yecP , and gntU promoter regions by using a pair of primers, 5′-fluorescein isothiocyanate (FITC)-labeled FITCT7pro primer (5′-TAATACGACTCACTATAGGG-3′) and T7-R primer (5′-GGTTTTCCCAGTCACACGACG-3′), SELEX fragment-containing plasmids (100 ng) as the template, and Ex Taq DNA polymerase (Takara). .. PCR products with FITC at their termini were purified by PAGE.

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
    Article Snippet: .. When TaKaRa Ex Taq™ polymerase was used, PCR with DNA preparation methods (A), (B), (C) and (D) generated high amount of PCR products at 40 cycles and the agarose gel electrophoresis showed clear bands of the PCR products. .. However, PCR with DNA preparation method (E) did not generate enough amount of PCR products at 40 cycles and the agarose gel electrophoresis showed no band of the PCR product ( ).

    Amplification:

    Article Title: RNA sequencing of the nephron transcriptome: a technical note
    Article Snippet: .. Finally, this cDNA molecule is amplified (the first-round PCR, 18–20 cycles) using the same universal primers (UP1 and UP2) and a high-performance DNA polymerase [TaKaRa Ex Taq HS DNA polymerase (Clontech Laboratories, Mountain View, CA, USA)]. .. Once the first-round PCR is complete, a quantitative real-time PCR (qRT-PCR) for a housekeeping gene (e.g., glutaraldehyde-3-dehydrogenase, beta-actin) is performed to see if the reverse transcription and amplification are successful.

    Article Title: Viability of an Escherichia coli pgsA Null Mutant Lacking Detectable Phosphatidylglycerol and Cardiolipin
    Article Snippet: .. DNA from fresh colonies of the strains to be examined was used as a template for PCR amplification with Ex Taq DNA polymerase (Takara, Tokyo, Japan). ..

    Agarose Gel Electrophoresis:

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
    Article Snippet: .. When TaKaRa Ex Taq™ polymerase was used, PCR with DNA preparation methods (A), (B), (C) and (D) generated high amount of PCR products at 40 cycles and the agarose gel electrophoresis showed clear bands of the PCR products. .. However, PCR with DNA preparation method (E) did not generate enough amount of PCR products at 40 cycles and the agarose gel electrophoresis showed no band of the PCR product ( ).

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    TaKaRa pcr master mix
    cDNA sequence of mRNA de novo transcribed from <t>TNFSF9</t> in HepG2 clone 9-1 (A) HepG2 clone 9-1 cells were incubated with 500 ng/ml LPS for the indicated times. cDNA was synthesized from total RNA, and TNFSF9 expression was quantified by real-time <t>PCR</t> as described in “Materials and Methods”. (B) HepG2 cells were incubated with 500 ng/ml LPS in the presence of 5-ethynyl uridine (EU) for 16 hrs. EU-labeled RNA was isolated as described in “Materials and Methods”. TNFSF9 cDNA was amplified by PCR and the PCR products were isolated and sequenced. This experiment is representative of four independent experiments.
    Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/TaKaRa
    Average 99 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    TaKaRa taq dna polymerase
    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic <t>DNA.</t> PCR was performed on the isolated genomic DNA using <t>taq</t> DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/TaKaRa
    Average 99 stars, based on 2134 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    TaKaRa dna polymerase
    Long range ribosomal PCR Amplifications of the 3.5 kb target from Fall specimens with Dream <t>Taq</t> ™. M: <t>DNA</t> markers; 1: 104H78; 2: 104H81; 3: 104H82; 4: 104H83; 5: 104H84; 6: 104H85; 7: 104H86; 8: 104H87; 9: 104H88; 10: 104H89; 11: 104H90; NC: negative control. 1-7: Female; 8-11: Male.
    Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/TaKaRa
    Average 94 stars, based on 224 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    cDNA sequence of mRNA de novo transcribed from TNFSF9 in HepG2 clone 9-1 (A) HepG2 clone 9-1 cells were incubated with 500 ng/ml LPS for the indicated times. cDNA was synthesized from total RNA, and TNFSF9 expression was quantified by real-time PCR as described in “Materials and Methods”. (B) HepG2 cells were incubated with 500 ng/ml LPS in the presence of 5-ethynyl uridine (EU) for 16 hrs. EU-labeled RNA was isolated as described in “Materials and Methods”. TNFSF9 cDNA was amplified by PCR and the PCR products were isolated and sequenced. This experiment is representative of four independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: cDNA sequence of mRNA de novo transcribed from TNFSF9 in HepG2 clone 9-1 (A) HepG2 clone 9-1 cells were incubated with 500 ng/ml LPS for the indicated times. cDNA was synthesized from total RNA, and TNFSF9 expression was quantified by real-time PCR as described in “Materials and Methods”. (B) HepG2 cells were incubated with 500 ng/ml LPS in the presence of 5-ethynyl uridine (EU) for 16 hrs. EU-labeled RNA was isolated as described in “Materials and Methods”. TNFSF9 cDNA was amplified by PCR and the PCR products were isolated and sequenced. This experiment is representative of four independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Sequencing, Incubation, Synthesized, Expressing, Real-time Polymerase Chain Reaction, Labeling, Isolation, Amplification, Polymerase Chain Reaction

    DNA sequence of genomic TNFSF9 gene edited by CRISPR-Cas9 The TNFSF9 genes in genomic DNAs from wild type and mutated HepG2 cells were amplified by PCR. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the genomic TNFSF9 sequence. On the right are shown the DNA sequences around the region of the mutated triplet in the wildtype and mutant clones. This is one representative of five independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: DNA sequence of genomic TNFSF9 gene edited by CRISPR-Cas9 The TNFSF9 genes in genomic DNAs from wild type and mutated HepG2 cells were amplified by PCR. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the genomic TNFSF9 sequence. On the right are shown the DNA sequences around the region of the mutated triplet in the wildtype and mutant clones. This is one representative of five independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Sequencing, CRISPR, Amplification, Polymerase Chain Reaction, Isolation, Mutagenesis, Clone Assay

    Heteroduplex formation by the cDNA of de novo transcripts of TNFSF9 from HepG2 clone 9-1 Total RNA and EU-labeled RNAs were isolated from clone 9-1 HepG2 cells. cDNA was synthesized and the TNFSF9 cDNA was amplified by PCR as described in “Materials and Methods”. The PCR products were annealed and incubated in the presence or absence of T7E1 endonuclease, and the products were fractionated on 1.2% agarose gels and visualized under UV. This is one representative of three independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: Heteroduplex formation by the cDNA of de novo transcripts of TNFSF9 from HepG2 clone 9-1 Total RNA and EU-labeled RNAs were isolated from clone 9-1 HepG2 cells. cDNA was synthesized and the TNFSF9 cDNA was amplified by PCR as described in “Materials and Methods”. The PCR products were annealed and incubated in the presence or absence of T7E1 endonuclease, and the products were fractionated on 1.2% agarose gels and visualized under UV. This is one representative of three independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Labeling, Isolation, Synthesized, Amplification, Polymerase Chain Reaction, Incubation

    cDNA sequence of mRNA transcribed from mutant genomic TNFSF9 TNFSF9 cDNA was synthesized and amplified by PCR from total RNA extracted from wild type and mutated HepG2 cells. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the TNFSF9 cDNA sequences. On the right are shown the cDNA sequences around the region of the mutated triplet in the wild type and mutant clones. This is one representative of four independent experiments.

    Journal: Molecules and Cells

    Article Title: Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript

    doi: 10.14348/molcells.2018.0209

    Figure Lengend Snippet: cDNA sequence of mRNA transcribed from mutant genomic TNFSF9 TNFSF9 cDNA was synthesized and amplified by PCR from total RNA extracted from wild type and mutated HepG2 cells. The PCR products were isolated and sequenced as described in “Materials and Methods”. Left panels are chromatograms of the TNFSF9 cDNA sequences. On the right are shown the cDNA sequences around the region of the mutated triplet in the wild type and mutant clones. This is one representative of four independent experiments.

    Article Snippet: Real-Time PCR amplification of TNFSF9 cDNA Real-time PCR analysis (MiniOpticon, Bio Rad, USA) was performed using PCR Master Mix (Takara SYBR® PreMix Ex Taq II) to quantify expression of TNFSF9 mRNA (normalized to β-actin expression).

    Techniques: Sequencing, Mutagenesis, Synthesized, Amplification, Polymerase Chain Reaction, Isolation, Clone Assay

    Effects of TGF-β1 on cell morphology, E-cadherin expression, and changes in N -glycans in GE11 cells. GE11 cells were grown in 6-well (2 × 10 5 ) or bottom dishes (2 × 10 4 ) for 24 h and then replaced with fresh complete medium with or without TGF-β1 (5 ng/ml) for another 4 days of incubation. A, cell morphology of the indicated cells was photographed. Photographs were taken of living cells using a ×10 objective. Scale bar, 100 μm. B, E-cadherin expressed on the cell surface was stained with anti-E-cadherin primary antibody, followed by incubation with Alexa Fluor-conjugated secondary antibody. Scale bar, 25 μm. C, total expression levels of E-cadherin were analyzed using Western blotting. Cell lysates from those cells were immunoblotted with anti-E-cadherin antibody. D, equal amounts of cell lysate proteins (20 μg) were used as the enzymatic source for the examination of GnT-III activities. S, substrate; P, product. E, mRNA expression of GnT-III . Quantitative RT-PCR was performed by monitoring in real time the increase in fluorescence of the SYBR Green dye on an ABI StepOnePlus. The mean number of cycles to the threshold ( CT ) of fluorescence detection was calculated for each sample, and the results were normalized to the mean CT of GAPDH for each sample tested. The changes in N -glycans were detected by E4-PHA ( F ) and concanavalin A ( ConA ) ( G ) lectin blot. α-Tubulin was used as a load control. H, N -glycans of GE11 cells cultured under normal conditions and treated with ( bottom ) or without ( top ) TGF-β for 4 days were released with peptide: N -glycosidase F ( PNGaseF ), as described under “Experimental Procedures,” digested with sialidase, and subjected to reversed-phase HPLC. The elution times for those PA-bisected N -glycans were compared with standard PA- N -glycans.

    Journal: The Journal of Biological Chemistry

    Article Title: Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor ?1 (TGF-?1) in Epithelial Cell Lines *

    doi: 10.1074/jbc.M111.262154

    Figure Lengend Snippet: Effects of TGF-β1 on cell morphology, E-cadherin expression, and changes in N -glycans in GE11 cells. GE11 cells were grown in 6-well (2 × 10 5 ) or bottom dishes (2 × 10 4 ) for 24 h and then replaced with fresh complete medium with or without TGF-β1 (5 ng/ml) for another 4 days of incubation. A, cell morphology of the indicated cells was photographed. Photographs were taken of living cells using a ×10 objective. Scale bar, 100 μm. B, E-cadherin expressed on the cell surface was stained with anti-E-cadherin primary antibody, followed by incubation with Alexa Fluor-conjugated secondary antibody. Scale bar, 25 μm. C, total expression levels of E-cadherin were analyzed using Western blotting. Cell lysates from those cells were immunoblotted with anti-E-cadherin antibody. D, equal amounts of cell lysate proteins (20 μg) were used as the enzymatic source for the examination of GnT-III activities. S, substrate; P, product. E, mRNA expression of GnT-III . Quantitative RT-PCR was performed by monitoring in real time the increase in fluorescence of the SYBR Green dye on an ABI StepOnePlus. The mean number of cycles to the threshold ( CT ) of fluorescence detection was calculated for each sample, and the results were normalized to the mean CT of GAPDH for each sample tested. The changes in N -glycans were detected by E4-PHA ( F ) and concanavalin A ( ConA ) ( G ) lectin blot. α-Tubulin was used as a load control. H, N -glycans of GE11 cells cultured under normal conditions and treated with ( bottom ) or without ( top ) TGF-β for 4 days were released with peptide: N -glycosidase F ( PNGaseF ), as described under “Experimental Procedures,” digested with sialidase, and subjected to reversed-phase HPLC. The elution times for those PA-bisected N -glycans were compared with standard PA- N -glycans.

    Article Snippet: Real time PCR was performed with a StepOnePlus real time PCR system (Applied Biosystems, Inc., Foster City, CA) using SYBR® Premix Ex TaqTM II PCR master mixes (Takara, Japan).

    Techniques: Expressing, Incubation, Staining, Western Blot, Quantitative RT-PCR, Fluorescence, SYBR Green Assay, Cell Culture, High Performance Liquid Chromatography

    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Journal: Neuroscience letters

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain

    doi: 10.1016/j.neulet.2010.12.060

    Figure Lengend Snippet: (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Techniques: Mutagenesis, Mouse Assay, Polymerase Chain Reaction, Isolation

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Fall specimens with Dream Taq ™. M: DNA markers; 1: 104H78; 2: 104H81; 3: 104H82; 4: 104H83; 5: 104H84; 6: 104H85; 7: 104H86; 8: 104H87; 9: 104H88; 10: 104H89; 11: 104H90; NC: negative control. 1-7: Female; 8-11: Male.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Fall specimens with Dream Taq ™. M: DNA markers; 1: 104H78; 2: 104H81; 3: 104H82; 4: 104H83; 5: 104H84; 6: 104H85; 7: 104H86; 8: 104H87; 9: 104H88; 10: 104H89; 11: 104H90; NC: negative control. 1-7: Female; 8-11: Male.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR performance of TaKaRa Ex Taq ® system and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. A: 1, 2, 3 and 4: TaKaRa Ex Taq ® system; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System; B: 1, 2, 3 and 4: TaKaRa Ex Taq ® system and Dream Taq ™; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System. NC: negative control, respectively.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of TaKaRa Ex Taq ® system and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. A: 1, 2, 3 and 4: TaKaRa Ex Taq ® system; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System; B: 1, 2, 3 and 4: TaKaRa Ex Taq ® system and Dream Taq ™; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System. NC: negative control, respectively.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ or/and Pfu in PicoMaxx™ buffer. M: DNA markers; 1, 2, 3 and 4: Dream Taq ™; 5, 6, 7 and 8: Pfu ; 9, 10, 11and 12: Dream Taq ™ and Pfu combined; 1, 5 and 9: 104K29; 2, 6 and 10: 104K30; 3, 7 and 11: 104K31; 4, 8 and 12: negative control (NC), respectively.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ or/and Pfu in PicoMaxx™ buffer. M: DNA markers; 1, 2, 3 and 4: Dream Taq ™; 5, 6, 7 and 8: Pfu ; 9, 10, 11and 12: Dream Taq ™ and Pfu combined; 1, 5 and 9: 104K29; 2, 6 and 10: 104K30; 3, 7 and 11: 104K31; 4, 8 and 12: negative control (NC), respectively.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with both Dream Taq ™ and Pfu in manufacturer’s PCR buffers. M: DNA markers; 1: 104K29; 2: 104K30; 3: 104K31; NC: negative control, respectively. A: Dream Taq ™ PCR buffer; B: Pfu PCR buffer.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with both Dream Taq ™ and Pfu in manufacturer’s PCR buffers. M: DNA markers; 1: 104K29; 2: 104K30; 3: 104K31; NC: negative control, respectively. A: Dream Taq ™ PCR buffer; B: Pfu PCR buffer.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with TaKaRa Ex Taq ® system. M: DNA markers; 1: 104J54; 2: 104J55; 3: 104J58; 4: 104J59; NC: negative control, respectively. A: Dream Taq ™; B: 18 S locus (1.7 kb) by Dream Taq ™, C: ITS and 28 S loci (1.9 kb) by Dream Taq ™; D: TaKaRa Ex Taq ® system.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with TaKaRa Ex Taq ® system. M: DNA markers; 1: 104J54; 2: 104J55; 3: 104J58; 4: 104J59; NC: negative control, respectively. A: Dream Taq ™; B: 18 S locus (1.7 kb) by Dream Taq ™, C: ITS and 28 S loci (1.9 kb) by Dream Taq ™; D: TaKaRa Ex Taq ® system.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR performance of Pfu and Pwo in PicoMaxx™ buffer. M: DNA markers; 1 and 4: 104K37; 2 and 5: 104K38; 3 and 6: 104K39. A: 1, 2 and 3: Dream Taq ™; 4, 5 and 6: Pwo (0.125 μl per reaction). B: 1, 2 and 3: Dream Taq ™ and Pfu ; 4, 5 and 6: Dream Taq ™ and Pwo (0.125 μl per reaction). NC: negative control, respectively. Note: final concentration of Pfu in each reaction was aligned with Pwo and Dream Taq ™ in 0.625 units.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of Pfu and Pwo in PicoMaxx™ buffer. M: DNA markers; 1 and 4: 104K37; 2 and 5: 104K38; 3 and 6: 104K39. A: 1, 2 and 3: Dream Taq ™; 4, 5 and 6: Pwo (0.125 μl per reaction). B: 1, 2 and 3: Dream Taq ™ and Pfu ; 4, 5 and 6: Dream Taq ™ and Pwo (0.125 μl per reaction). NC: negative control, respectively. Note: final concentration of Pfu in each reaction was aligned with Pwo and Dream Taq ™ in 0.625 units.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control, Concentration Assay

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: 18 S locus (1.7 kb) by Dream Taq ™, B: ITS and 28 S loci (1.9 kb) by Dream Taq ™; C: Dream Taq ™ and PicoMaxx™ High Fidelity PCR System combined.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: 18 S locus (1.7 kb) by Dream Taq ™, B: ITS and 28 S loci (1.9 kb) by Dream Taq ™; C: Dream Taq ™ and PicoMaxx™ High Fidelity PCR System combined.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR performance of Taq 2000™, Platinum™ Taq and Dream Taq ™. M: DNA markers; 1, 4 and 7: 104N95; 2, 5 and 8: 104N96; 3, 6 and 9: 104N97. 1, 2, 3 and NC by Taq 2000™; 4, 5, 6 and NC by Platinum™ Taq ; 7, 8, 9 and NC by Dream Taq ™, NC: negative control, respectively. A: 3.5 kb target; B: 1.9 kb ITS and 28 S target. Note: final concentration of either Taq 2000™ or Dream Taq ™ in each reaction was aligned with Platinum™ Taq in 1.25 units.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of Taq 2000™, Platinum™ Taq and Dream Taq ™. M: DNA markers; 1, 4 and 7: 104N95; 2, 5 and 8: 104N96; 3, 6 and 9: 104N97. 1, 2, 3 and NC by Taq 2000™; 4, 5, 6 and NC by Platinum™ Taq ; 7, 8, 9 and NC by Dream Taq ™, NC: negative control, respectively. A: 3.5 kb target; B: 1.9 kb ITS and 28 S target. Note: final concentration of either Taq 2000™ or Dream Taq ™ in each reaction was aligned with Platinum™ Taq in 1.25 units.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control, Concentration Assay

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K17; 2: 104K18; 3: 104K19; 4: 104K20; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K17; 2: 104K18; 3: 104K19; 4: 104K20; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control