ex taq dna polymerase (TaKaRa)


Name:
TaKaRa Taq DNA Polymerase
Description:
TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT 1 strain and is suitable for routine PCR applications
Catalog Number:
r001c
Price:
None
Category:
Takara Taq and premix Takara Taq products Standard PCR PCR
Size:
3 000 Units
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Structured Review
TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT 1 strain and is suitable for routine PCR applications
https://www.bioz.com/result/ex taq dna polymerase/product/TaKaRa
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Amplification:Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples Article Snippet: .. Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome Article Snippet: The genomic DNAs from three apes and 12 related chimpanzee individuals were kindly provided by Dr. Takenaka (Primate Research Institute, Kyoto University). .. We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of Polymerase Chain Reaction:Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples Article Snippet: .. Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples Article Snippet: .. First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using Article Title: Down-Regulation of DUSP6 Expression in Lung Cancer --Its Mechanism and Potential Role in Carcinogenesis Article Snippet: Genomic DNA was extracted from cultured cells as described previously, and subjected to bisulfate conversion treatment using a MethylEasy DNA bisulfate modification kit (Human Genetic Signatures, Macquarie Park, Australia) according to the manufacturer’s instructions. .. The region from −960 to −266 of the DUSP6 gene, including the promoter locus, was Article Title: RNAi in Cultured Drosophila Cells Article Snippet: Oligonucleotide primers for PCR. .. Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome Article Snippet: The genomic DNAs from three apes and 12 related chimpanzee individuals were kindly provided by Dr. Takenaka (Primate Research Institute, Kyoto University). .. We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of Isolation:Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using Chloramphenicol Acetyltransferase Assay:Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using Activated Clotting Time Assay:Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using DNA Synthesis:Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease Article Snippet: Time course experiments were performed in the same reaction mixture, except for the enzyme amount (92 fmol), by varying the incubation time (0, 30, 60, 90 min). .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Activity Assay:Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease Article Snippet: Time course experiments were performed in the same reaction mixture, except for the enzyme amount (92 fmol), by varying the incubation time (0, 30, 60, 90 min). .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Software:Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome Article Snippet: The genomic DNAs from three apes and 12 related chimpanzee individuals were kindly provided by Dr. Takenaka (Primate Research Institute, Kyoto University). .. We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of |