ex taq dna polymerase  (TaKaRa)


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    Name:
    TaKaRa Taq DNA Polymerase
    Description:
    TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT 1 strain and is suitable for routine PCR applications
    Catalog Number:
    r001c
    Price:
    None
    Category:
    Takara Taq and premix Takara Taq products Standard PCR PCR
    Size:
    3 000 Units
    		Buy from Supplier

    Structured Review

    TaKaRa ex taq dna polymerase
    TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT 1 strain and is suitable for routine PCR applications
    https://www.bioz.com/result/ex taq dna polymerase/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ex taq dna polymerase - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome
    Article Snippet: The genomic DNAs from three apes and 12 related chimpanzee individuals were kindly provided by Dr. Takenaka (Primate Research Institute, Kyoto University). .. We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of PCR master mix containing DNA polymerase (TaKaRa EmeraldAmp GT PCR Master Mix, TaKaRa, Japan). .. Each PCR amplification was started with an denaturation step of 5 min at 95°C, followed by 35 cycles of 30 sec of denaturation at 95°C, 30 sec of annealing temperature, and 1 to 3 min of extension (depending on the expected size of PCR product) at 72°C, followed by a final extension at 72°C for 5 min. Two microliters of the resulting PCR products were loaded on 1% agarose gel, stained with ethidium bromide, and visualized using UV fluorescence.

    Polymerase Chain Reaction:

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Article Title: Down-Regulation of DUSP6 Expression in Lung Cancer --Its Mechanism and Potential Role in Carcinogenesis
    Article Snippet: Genomic DNA was extracted from cultured cells as described previously, and subjected to bisulfate conversion treatment using a MethylEasy DNA bisulfate modification kit (Human Genetic Signatures, Macquarie Park, Australia) according to the manufacturer’s instructions. .. The region from −960 to −266 of the DUSP6 gene, including the promoter locus, was PCR-amplified (TaqHS DNA polymerase, Takara) with three sets of primers (F1, 5′-GAGTTGGGTTTTTAAAGTGGTAAATA-3′ and R1, 5′-CAAAACACATAAACCAAAACACTTC-3′; F2, 5′-GAAATATGAGATAATTGAAGTGTTTTGG-3′ and R2, 5′-CAACTCCTCAATAAATACAAACAAC-3′; F3, 5′-TGTTTGTATTTATTGAGGAGTTGTTT-3′ and R3, 5′-CTACCCAAATAATTTTTATTCCTCC-3′), and subcloned into pT7Blue (Novagen). .. Eight clones were randomly chosen, and nucleotide conversions at CpG sites were searched for, by DNA sequencing (Dye-deoxy DNA sequencing kit, Amersham).

    Article Title: RNAi in Cultured Drosophila Cells
    Article Snippet: Oligonucleotide primers for PCR. .. Thermostable DNA polymerase (e.g., Clontech Advantage2 PCR reagent). .. 10X buffer for PCR.

    Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome
    Article Snippet: The genomic DNAs from three apes and 12 related chimpanzee individuals were kindly provided by Dr. Takenaka (Primate Research Institute, Kyoto University). .. We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of PCR master mix containing DNA polymerase (TaKaRa EmeraldAmp GT PCR Master Mix, TaKaRa, Japan). .. Each PCR amplification was started with an denaturation step of 5 min at 95°C, followed by 35 cycles of 30 sec of denaturation at 95°C, 30 sec of annealing temperature, and 1 to 3 min of extension (depending on the expected size of PCR product) at 72°C, followed by a final extension at 72°C for 5 min. Two microliters of the resulting PCR products were loaded on 1% agarose gel, stained with ethidium bromide, and visualized using UV fluorescence.

    Isolation:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Activated Clotting Time Assay:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: Each genotype was examined by regular three primer polymerase chain reaction (PCR) systems. .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    DNA Synthesis:

    Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
    Article Snippet: Time course experiments were performed in the same reaction mixture, except for the enzyme amount (92 fmol), by varying the incubation time (0, 30, 60, 90 min). .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

    Activity Assay:

    Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
    Article Snippet: Time course experiments were performed in the same reaction mixture, except for the enzyme amount (92 fmol), by varying the incubation time (0, 30, 60, 90 min). .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

    Software:

    Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome
    Article Snippet: The genomic DNAs from three apes and 12 related chimpanzee individuals were kindly provided by Dr. Takenaka (Primate Research Institute, Kyoto University). .. We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of PCR master mix containing DNA polymerase (TaKaRa EmeraldAmp GT PCR Master Mix, TaKaRa, Japan). .. Each PCR amplification was started with an denaturation step of 5 min at 95°C, followed by 35 cycles of 30 sec of denaturation at 95°C, 30 sec of annealing temperature, and 1 to 3 min of extension (depending on the expected size of PCR product) at 72°C, followed by a final extension at 72°C for 5 min. Two microliters of the resulting PCR products were loaded on 1% agarose gel, stained with ethidium bromide, and visualized using UV fluorescence.

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    • taq dna polymerase  (TaKaRa)
    99
    TaKaRa taq dna polymerase
    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic <t>DNA.</t> PCR was performed on the isolated genomic DNA using <t>taq</t> DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier
    dna polymerase  (TaKaRa)
    99
    TaKaRa dna polymerase
    Long range ribosomal PCR Amplifications of the 3.5 kb target from Fall specimens with Dream <t>Taq</t> ™. M: <t>DNA</t> markers; 1: 104H78; 2: 104H81; 3: 104H82; 4: 104H83; 5: 104H84; 6: 104H85; 7: 104H86; 8: 104H87; 9: 104H88; 10: 104H89; 11: 104H90; NC: negative control. 1-7: Female; 8-11: Male.
    Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2021-03
    99/100 stars
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    ex taq dna polymerase  (TaKaRa)
    97
    TaKaRa ex taq dna polymerase
    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with <t>Taq</t> I. Lane M: 100 bp <t>DNA</t> ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
    Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ex taq dna polymerase/product/TaKaRa
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ex taq dna polymerase - by Bioz Stars, 2021-03
    97/100 stars
    		  Buy from Supplier

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    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Journal: Neuroscience letters

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain

    doi: 10.1016/j.neulet.2010.12.060

    Figure Lengend Snippet: (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Techniques: Mutagenesis, Mouse Assay, Polymerase Chain Reaction, Isolation

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Fall specimens with Dream Taq ™. M: DNA markers; 1: 104H78; 2: 104H81; 3: 104H82; 4: 104H83; 5: 104H84; 6: 104H85; 7: 104H86; 8: 104H87; 9: 104H88; 10: 104H89; 11: 104H90; NC: negative control. 1-7: Female; 8-11: Male.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Fall specimens with Dream Taq ™. M: DNA markers; 1: 104H78; 2: 104H81; 3: 104H82; 4: 104H83; 5: 104H84; 6: 104H85; 7: 104H86; 8: 104H87; 9: 104H88; 10: 104H89; 11: 104H90; NC: negative control. 1-7: Female; 8-11: Male.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR performance of TaKaRa Ex Taq ® system and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. A: 1, 2, 3 and 4: TaKaRa Ex Taq ® system; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System; B: 1, 2, 3 and 4: TaKaRa Ex Taq ® system and Dream Taq ™; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System. NC: negative control, respectively.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of TaKaRa Ex Taq ® system and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1 and 5: 104K37; 2 and 6: 104K38; 3 and 7: 104K39; 4 and 8: 104K40. A: 1, 2, 3 and 4: TaKaRa Ex Taq ® system; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System; B: 1, 2, 3 and 4: TaKaRa Ex Taq ® system and Dream Taq ™; 5, 6, 7 and 8: PicoMaxx™ High Fidelity PCR System. NC: negative control, respectively.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ or/and Pfu in PicoMaxx™ buffer. M: DNA markers; 1, 2, 3 and 4: Dream Taq ™; 5, 6, 7 and 8: Pfu ; 9, 10, 11and 12: Dream Taq ™ and Pfu combined; 1, 5 and 9: 104K29; 2, 6 and 10: 104K30; 3, 7 and 11: 104K31; 4, 8 and 12: negative control (NC), respectively.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ or/and Pfu in PicoMaxx™ buffer. M: DNA markers; 1, 2, 3 and 4: Dream Taq ™; 5, 6, 7 and 8: Pfu ; 9, 10, 11and 12: Dream Taq ™ and Pfu combined; 1, 5 and 9: 104K29; 2, 6 and 10: 104K30; 3, 7 and 11: 104K31; 4, 8 and 12: negative control (NC), respectively.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with both Dream Taq ™ and Pfu in manufacturer’s PCR buffers. M: DNA markers; 1: 104K29; 2: 104K30; 3: 104K31; NC: negative control, respectively. A: Dream Taq ™ PCR buffer; B: Pfu PCR buffer.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with both Dream Taq ™ and Pfu in manufacturer’s PCR buffers. M: DNA markers; 1: 104K29; 2: 104K30; 3: 104K31; NC: negative control, respectively. A: Dream Taq ™ PCR buffer; B: Pfu PCR buffer.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with TaKaRa Ex Taq ® system. M: DNA markers; 1: 104J54; 2: 104J55; 3: 104J58; 4: 104J59; NC: negative control, respectively. A: Dream Taq ™; B: 18 S locus (1.7 kb) by Dream Taq ™, C: ITS and 28 S loci (1.9 kb) by Dream Taq ™; D: TaKaRa Ex Taq ® system.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with TaKaRa Ex Taq ® system. M: DNA markers; 1: 104J54; 2: 104J55; 3: 104J58; 4: 104J59; NC: negative control, respectively. A: Dream Taq ™; B: 18 S locus (1.7 kb) by Dream Taq ™, C: ITS and 28 S loci (1.9 kb) by Dream Taq ™; D: TaKaRa Ex Taq ® system.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR performance of Pfu and Pwo in PicoMaxx™ buffer. M: DNA markers; 1 and 4: 104K37; 2 and 5: 104K38; 3 and 6: 104K39. A: 1, 2 and 3: Dream Taq ™; 4, 5 and 6: Pwo (0.125 μl per reaction). B: 1, 2 and 3: Dream Taq ™ and Pfu ; 4, 5 and 6: Dream Taq ™ and Pwo (0.125 μl per reaction). NC: negative control, respectively. Note: final concentration of Pfu in each reaction was aligned with Pwo and Dream Taq ™ in 0.625 units.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of Pfu and Pwo in PicoMaxx™ buffer. M: DNA markers; 1 and 4: 104K37; 2 and 5: 104K38; 3 and 6: 104K39. A: 1, 2 and 3: Dream Taq ™; 4, 5 and 6: Pwo (0.125 μl per reaction). B: 1, 2 and 3: Dream Taq ™ and Pfu ; 4, 5 and 6: Dream Taq ™ and Pwo (0.125 μl per reaction). NC: negative control, respectively. Note: final concentration of Pfu in each reaction was aligned with Pwo and Dream Taq ™ in 0.625 units.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control, Concentration Assay

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: 18 S locus (1.7 kb) by Dream Taq ™, B: ITS and 28 S loci (1.9 kb) by Dream Taq ™; C: Dream Taq ™ and PicoMaxx™ High Fidelity PCR System combined.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with Dream Taq ™ and PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: 18 S locus (1.7 kb) by Dream Taq ™, B: ITS and 28 S loci (1.9 kb) by Dream Taq ™; C: Dream Taq ™ and PicoMaxx™ High Fidelity PCR System combined.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR performance of Taq 2000™, Platinum™ Taq and Dream Taq ™. M: DNA markers; 1, 4 and 7: 104N95; 2, 5 and 8: 104N96; 3, 6 and 9: 104N97. 1, 2, 3 and NC by Taq 2000™; 4, 5, 6 and NC by Platinum™ Taq ; 7, 8, 9 and NC by Dream Taq ™, NC: negative control, respectively. A: 3.5 kb target; B: 1.9 kb ITS and 28 S target. Note: final concentration of either Taq 2000™ or Dream Taq ™ in each reaction was aligned with Platinum™ Taq in 1.25 units.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: PCR performance of Taq 2000™, Platinum™ Taq and Dream Taq ™. M: DNA markers; 1, 4 and 7: 104N95; 2, 5 and 8: 104N96; 3, 6 and 9: 104N97. 1, 2, 3 and NC by Taq 2000™; 4, 5, 6 and NC by Platinum™ Taq ; 7, 8, 9 and NC by Dream Taq ™, NC: negative control, respectively. A: 3.5 kb target; B: 1.9 kb ITS and 28 S target. Note: final concentration of either Taq 2000™ or Dream Taq ™ in each reaction was aligned with Platinum™ Taq in 1.25 units.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control, Concentration Assay

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K17; 2: 104K18; 3: 104K19; 4: 104K20; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K17; 2: 104K18; 3: 104K19; 4: 104K20; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Journal: Journal of Nematology

    Article Title: Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

    doi: 10.21307/jofnem-2020-016

    Figure Lengend Snippet: Long range ribosomal PCR Amplifications of the 3.5 kb target from Summer specimens with PicoMaxx™ High Fidelity PCR System. M: DNA markers; 1: 104K25; 2: 104K26; 3: 104K27; 4: 104K28; 5: 104K29; 6: 104K30; 7: 104K31; NC: negative control, respectively. A: Dream Taq ™; B: PicoMaxx™ High Fidelity PCR System.

    Article Snippet: This failure can be prevented by a proofreading DNA polymerase (either TaKaRa Ex Taq® system or the PicoMaxx™ system) in these Summer specimens ( and ).

    Techniques: Polymerase Chain Reaction, Negative Control

    PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.

    Journal: Journal of Veterinary Science

    Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates

    doi: 10.4142/jvs.2006.7.3.277

    Figure Lengend Snippet: PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.

    Article Snippet: The PCR conditions consisted of 5 µl (50 ng/µl) of DNA and 1 µl each of primer (50 pM) in a 5 µl of 10× reaction buffer with 5 µl of 25 mM MgCl2 , 5 µl of 10 mM dNTP (each 2.5 mM) and 1 µl of 5 U Ex Taq DNA polymerase (TaKaRa, Japan) to a final volume of 50 µl on a thermal cycler (PTC-100; MJ Research, USA).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Proof-of-principle for AMhyMsd-PCR. JF1, B6, JF/B6 F1, and B6/JF F1 indicate JF1, B6NJ, and reciprocal F1 hybrids of JF and B6NJ mice, respectively. (a) Application of AMhyMsd-PCR to GR #10 with maternal ASM of Impact . AMhyMsd-PCR was applied to the maternally methylated CGI, GR #10, located in the first intron of Impact . Undigested, Hpa II, Lpn PI, Pvu RTS1l, Taq αI, and T4-BGT+ Taq αI indicate the PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Taq αI-, and T4-BGT+ Taq αI-digested DNAs, respectively. The PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Taq αI-, and T4-BGT+ Taq αI-digested DNAs from JF1, B6, JF/B6 F1, and B6/JF F1 mice were electrophoresed, stained with ethidium bromide, and visualized by UV illumination. (b) AMhyMsd-PCR can distinguish complete AShyM from ‘mixture of ASM and AShyM’. The ‘M’ and ‘P’ in each rounded rectangle indicate maternal and paternal alleles, respectively. The right panel shows parental alleles exempt from restriction enzyme digestion of the amplicon. (c) Application of AMhyMsd-PCR for GR #9, the Nhlrc1 promoter region with sd-AShyM. Undigested, Hpa II, Lpn PI, Pvu RTS1l, Msp I, and T4-BGT+ Msp I indicate the PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Msp I- and T4-BGT+ Msp I-digested DNAs, respectively. The PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Msp I-, or T4-BGT+ Msp I-digested DNAs from JF/B6 F1 or B6/JF F1 mice were electrophoresed and stained with ethidium bromide. (d) Direct sequencing of AMhyMsd-PCR products from GR #9 in a JF/B6 F1 hybrid. The vertical arrowhead shows the SNP site (rs48369422: C/T). (e) Direct sequencing of AMhyMsd-PCR products from GR #9 in a B6/JF F1 hybrid. The vertical arrowhead shows the SNP site (rs48369422: C/T). (f) Bisulphite sequencing of GR #9 in JF/B6 and B6/JF F1 hybrids. Each row of circles indicates a clone of bisulphite PCR products. Open and closed circles show unmethylated and methylated CpG sites, respectively. The CpG sites within Msp I recognition sequences (CCGG) in the AMhyMsd-PCR amplicon are indicated by vertical arrowheads. The SNP site (rs48369422: C/T) is indicated by a vertical arrowhead. (g) Quantitative PCR targeting GR #9 in undigested and each restriction enzyme-digested DNAs from a B6/JF F1 hybrid. Undigestion, HpaII, MspI, and T4-BGT+MspI indicate enzyme-resistant DNA levels of GR #9 in undigested, Hpa II-, Msp I- and T4-BGT+ Msp I-digested DNAs, respectively.

    Journal: Epigenetics

    Article Title: A method for identifying allele-specific hydroxymethylation

    doi: 10.1080/15592294.2019.1664228

    Figure Lengend Snippet: Proof-of-principle for AMhyMsd-PCR. JF1, B6, JF/B6 F1, and B6/JF F1 indicate JF1, B6NJ, and reciprocal F1 hybrids of JF and B6NJ mice, respectively. (a) Application of AMhyMsd-PCR to GR #10 with maternal ASM of Impact . AMhyMsd-PCR was applied to the maternally methylated CGI, GR #10, located in the first intron of Impact . Undigested, Hpa II, Lpn PI, Pvu RTS1l, Taq αI, and T4-BGT+ Taq αI indicate the PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Taq αI-, and T4-BGT+ Taq αI-digested DNAs, respectively. The PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Taq αI-, and T4-BGT+ Taq αI-digested DNAs from JF1, B6, JF/B6 F1, and B6/JF F1 mice were electrophoresed, stained with ethidium bromide, and visualized by UV illumination. (b) AMhyMsd-PCR can distinguish complete AShyM from ‘mixture of ASM and AShyM’. The ‘M’ and ‘P’ in each rounded rectangle indicate maternal and paternal alleles, respectively. The right panel shows parental alleles exempt from restriction enzyme digestion of the amplicon. (c) Application of AMhyMsd-PCR for GR #9, the Nhlrc1 promoter region with sd-AShyM. Undigested, Hpa II, Lpn PI, Pvu RTS1l, Msp I, and T4-BGT+ Msp I indicate the PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Msp I- and T4-BGT+ Msp I-digested DNAs, respectively. The PCR products from undigested, Hpa II-, Lpn PI-, Pvu RTS1l-, Msp I-, or T4-BGT+ Msp I-digested DNAs from JF/B6 F1 or B6/JF F1 mice were electrophoresed and stained with ethidium bromide. (d) Direct sequencing of AMhyMsd-PCR products from GR #9 in a JF/B6 F1 hybrid. The vertical arrowhead shows the SNP site (rs48369422: C/T). (e) Direct sequencing of AMhyMsd-PCR products from GR #9 in a B6/JF F1 hybrid. The vertical arrowhead shows the SNP site (rs48369422: C/T). (f) Bisulphite sequencing of GR #9 in JF/B6 and B6/JF F1 hybrids. Each row of circles indicates a clone of bisulphite PCR products. Open and closed circles show unmethylated and methylated CpG sites, respectively. The CpG sites within Msp I recognition sequences (CCGG) in the AMhyMsd-PCR amplicon are indicated by vertical arrowheads. The SNP site (rs48369422: C/T) is indicated by a vertical arrowhead. (g) Quantitative PCR targeting GR #9 in undigested and each restriction enzyme-digested DNAs from a B6/JF F1 hybrid. Undigestion, HpaII, MspI, and T4-BGT+MspI indicate enzyme-resistant DNA levels of GR #9 in undigested, Hpa II-, Msp I- and T4-BGT+ Msp I-digested DNAs, respectively.

    Article Snippet: One-tenth of the bisulphite-treated DNA was used for PCR in a 10-μL of the recommended buffer containing 0.5 U of Ex-Taq DNA polymerase (TaKaRa) and 2.5 pmols of each primer.

    Techniques: Polymerase Chain Reaction, Mouse Assay, Methylation, Staining, Amplification, Sequencing, Bisulfite Sequencing, Real-time Polymerase Chain Reaction