etoposide eto  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Etoposide
    Description:
    Etoposide is synthesised from podophyllotoxins of plants
    Catalog Number:
    e1383
    Price:
    None
    Applications:
    Etoposide has been used:. to prepare drug stock solution in dimethyl sulfoxide (DMSO) and also to profile and compare the sensitivity of DT40 mutant cells. to incubate cells for cell viability assay . to treat neuro-2A cells to induce programmed cell death
    Buy from Supplier


    Structured Review

    Millipore etoposide eto
    Etoposide
    Etoposide is synthesised from podophyllotoxins of plants
    https://www.bioz.com/result/etoposide eto/product/Millipore
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    etoposide eto - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Actin Polymerization Negatively Regulates p53 Function by Impairing Its Nuclear Import in Response to DNA Damage"

    Article Title: Actin Polymerization Negatively Regulates p53 Function by Impairing Its Nuclear Import in Response to DNA Damage

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0060179

    Actin polymerization increases in response to DNA damage. A. U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h, and images were captured at the indicated time points. B. The cell length and width were analyzed with Image J software in ≥100 cells per condition. C . U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h, the intensity of phalloidin was measured with Image Pro Plus software. Scale bar, 10 µm. D. U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h, and fluorescence assays were performed with a fluorescence microplate reader to measure cellular F-actin levels (phalloidin intensity/DAPI intensity). E. Cells were treated with ETO (10 µM) at indicated time points, and then whole cell extracts were analyzed by western blotting using anti-γH2AX antibody (a). U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h. Then, immunofluorescence was performed to detect the signal of γH2AX (b). Scale bar, 10 µm. All Statistical differences were determined by One-way ANOVA. Results are presented as means ± SD of values from three independent experiments. ETO, etoposide.
    Figure Legend Snippet: Actin polymerization increases in response to DNA damage. A. U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h, and images were captured at the indicated time points. B. The cell length and width were analyzed with Image J software in ≥100 cells per condition. C . U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h, the intensity of phalloidin was measured with Image Pro Plus software. Scale bar, 10 µm. D. U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h, and fluorescence assays were performed with a fluorescence microplate reader to measure cellular F-actin levels (phalloidin intensity/DAPI intensity). E. Cells were treated with ETO (10 µM) at indicated time points, and then whole cell extracts were analyzed by western blotting using anti-γH2AX antibody (a). U2OS cells were treated with ETO (10 µM) or untreated as control for 24 h. Then, immunofluorescence was performed to detect the signal of γH2AX (b). Scale bar, 10 µm. All Statistical differences were determined by One-way ANOVA. Results are presented as means ± SD of values from three independent experiments. ETO, etoposide.

    Techniques Used: Software, Fluorescence, Western Blot, Immunofluorescence

    2) Product Images from "Damage-associated molecular patterns (DAMPs) related to immunogenic cell death are differentially triggered by clinically relevant chemotherapeutics in lung adenocarcinoma cells"

    Article Title: Damage-associated molecular patterns (DAMPs) related to immunogenic cell death are differentially triggered by clinically relevant chemotherapeutics in lung adenocarcinoma cells

    Journal: BMC Cancer

    doi: 10.1186/s12885-020-06964-5

    Induction of apoptosis and phenotypic alterations in NSCLC cells by classic chemotherapeutics. a Effect of chemotherapeutics in the number of A549 cells after 48 h of treatment. C – control (DMSO not exceeding 0.1%); Cis – Cisplatin 40 μM; Eto – Etoposide 13.2 μM; Carb – Carboplatin 200 μM; Pac – Paclitaxel 100 nM; Gem – Gemcitabine 0.96 μM; Cis + Eto – Cisplatina 40 μM + Etoposide 13.2 μM; Carb+Pac – Carboplatin 200 μM + Paclitaxel 100 nM. Cell number was analyzed by cell counting in a flow cytometer. Data represent the mean ± SEM;* p
    Figure Legend Snippet: Induction of apoptosis and phenotypic alterations in NSCLC cells by classic chemotherapeutics. a Effect of chemotherapeutics in the number of A549 cells after 48 h of treatment. C – control (DMSO not exceeding 0.1%); Cis – Cisplatin 40 μM; Eto – Etoposide 13.2 μM; Carb – Carboplatin 200 μM; Pac – Paclitaxel 100 nM; Gem – Gemcitabine 0.96 μM; Cis + Eto – Cisplatina 40 μM + Etoposide 13.2 μM; Carb+Pac – Carboplatin 200 μM + Paclitaxel 100 nM. Cell number was analyzed by cell counting in a flow cytometer. Data represent the mean ± SEM;* p

    Techniques Used: Cell Counting, Flow Cytometry

    Related Articles

    Electroporation:

    Article Title: Ehrlichia type IV secretion effector ECH0825 is translocated to mitochondria and curbs ROS and apoptosis by upregulating host MnSOD
    Article Snippet: .. RF/6A or HEK293 cells were transfected by electroporation with Gene Pulser Xcell System (Bio-Rad, Hercules, CA) as described ( ). pEGFP-N1- or pECH0825-transfected RF/6A cells were seeded at 1 × 105 cells/ml into each well of a 6-well plate, and at 20 h post-transfection cells were treated with 100 μM etoposide (Sigma-Aldrich) for 1 day and fixed with 2% paraformaldehyde in PBS. .. The integrity of nuclei was determined by staining cells with 300 nM of 4’, 6-diamidino-2-phenylindole, dilactate (DAPI, Invitrogen) for 5 min, and cells were observed by fluorescence microscopy to quantify cellular apoptosis.

    Transfection:

    Article Title: Ehrlichia type IV secretion effector ECH0825 is translocated to mitochondria and curbs ROS and apoptosis by upregulating host MnSOD
    Article Snippet: .. RF/6A or HEK293 cells were transfected by electroporation with Gene Pulser Xcell System (Bio-Rad, Hercules, CA) as described ( ). pEGFP-N1- or pECH0825-transfected RF/6A cells were seeded at 1 × 105 cells/ml into each well of a 6-well plate, and at 20 h post-transfection cells were treated with 100 μM etoposide (Sigma-Aldrich) for 1 day and fixed with 2% paraformaldehyde in PBS. .. The integrity of nuclei was determined by staining cells with 300 nM of 4’, 6-diamidino-2-phenylindole, dilactate (DAPI, Invitrogen) for 5 min, and cells were observed by fluorescence microscopy to quantify cellular apoptosis.

    Mutagenesis:

    Article Title: Proteomic Profiling of S-acylated Macrophage Proteins Identifies a Role for Palmitoylation in Mitochondrial Targeting of Phospholipid Scramblase 3 *
    Article Snippet: .. Apoptosis in empty vector, wt Plscr3, and 5A- mutant Plscr3 stable RAW264.7 cells was determined after exposure to etoposide (Sigma) for 8 or 24 h. Cells were seeded at 0.75 million/well in a 12-well tissue culture plate 24 h prior to etoposide exposure in 0.1% DMSO. .. After exposure, media was removed and cells were incubated in 1 ml of Phenol Red-free DMEM (1% fetal calf serum) containing 1 μg/ml of the apoptosis probe, FAM-FLIVO (ImmunoChemistry Technologies, Bloomington, MN) per manufacturer's instructions.

    Concentration Assay:

    Article Title: Bone Marrow Mesenchymal Stem Cells for Improving Hematopoietic Function: An In Vitro and In Vivo Model. Part 2: Effect on Bone Marrow Microenvironment
    Article Snippet: .. Etoposide (Sigma-Aldrich, Steinheim, Germany) was reconstituted in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Steinheim, Germany) at a concentration of 20 mg/ml and stored at −20°C. ..

    other:

    Article Title: Signalling of the M3-muscarinic receptor to the anti-apoptotic pathway
    Article Snippet: Etoposide, and actinomycin D were from Calbiochem (Nottingham, U.K.).

    Plasmid Preparation:

    Article Title: Proteomic Profiling of S-acylated Macrophage Proteins Identifies a Role for Palmitoylation in Mitochondrial Targeting of Phospholipid Scramblase 3 *
    Article Snippet: .. Apoptosis in empty vector, wt Plscr3, and 5A- mutant Plscr3 stable RAW264.7 cells was determined after exposure to etoposide (Sigma) for 8 or 24 h. Cells were seeded at 0.75 million/well in a 12-well tissue culture plate 24 h prior to etoposide exposure in 0.1% DMSO. .. After exposure, media was removed and cells were incubated in 1 ml of Phenol Red-free DMEM (1% fetal calf serum) containing 1 μg/ml of the apoptosis probe, FAM-FLIVO (ImmunoChemistry Technologies, Bloomington, MN) per manufacturer's instructions.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore etoposide eto
    Induction of apoptosis and phenotypic alterations in NSCLC cells by classic chemotherapeutics. a Effect of chemotherapeutics in the number of A549 cells after 48 h of treatment. C – control (DMSO not exceeding 0.1%); Cis – Cisplatin 40 μM; <t>Eto</t> – <t>Etoposide</t> 13.2 μM; Carb – Carboplatin 200 μM; Pac – Paclitaxel 100 nM; Gem – Gemcitabine 0.96 μM; Cis + Eto – Cisplatina 40 μM + Etoposide 13.2 μM; Carb+Pac – Carboplatin 200 μM + Paclitaxel 100 nM. Cell number was analyzed by cell counting in a flow cytometer. Data represent the mean ± SEM;* p
    Etoposide Eto, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etoposide eto/product/Millipore
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    etoposide eto - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Induction of apoptosis and phenotypic alterations in NSCLC cells by classic chemotherapeutics. a Effect of chemotherapeutics in the number of A549 cells after 48 h of treatment. C – control (DMSO not exceeding 0.1%); Cis – Cisplatin 40 μM; Eto – Etoposide 13.2 μM; Carb – Carboplatin 200 μM; Pac – Paclitaxel 100 nM; Gem – Gemcitabine 0.96 μM; Cis + Eto – Cisplatina 40 μM + Etoposide 13.2 μM; Carb+Pac – Carboplatin 200 μM + Paclitaxel 100 nM. Cell number was analyzed by cell counting in a flow cytometer. Data represent the mean ± SEM;* p

    Journal: BMC Cancer

    Article Title: Damage-associated molecular patterns (DAMPs) related to immunogenic cell death are differentially triggered by clinically relevant chemotherapeutics in lung adenocarcinoma cells

    doi: 10.1186/s12885-020-06964-5

    Figure Lengend Snippet: Induction of apoptosis and phenotypic alterations in NSCLC cells by classic chemotherapeutics. a Effect of chemotherapeutics in the number of A549 cells after 48 h of treatment. C – control (DMSO not exceeding 0.1%); Cis – Cisplatin 40 μM; Eto – Etoposide 13.2 μM; Carb – Carboplatin 200 μM; Pac – Paclitaxel 100 nM; Gem – Gemcitabine 0.96 μM; Cis + Eto – Cisplatina 40 μM + Etoposide 13.2 μM; Carb+Pac – Carboplatin 200 μM + Paclitaxel 100 nM. Cell number was analyzed by cell counting in a flow cytometer. Data represent the mean ± SEM;* p

    Article Snippet: After 24 h of incubation, cells were treated for 48 h with cisplatin (Cis), etoposide (Eto), carboplatin (Carb), paclitaxel (Pac), gemcitabine (Gem) and also with co-treatments of Cis plus Eto and Carb plus Pac.

    Techniques: Cell Counting, Flow Cytometry