ethylenediaminetretraacetic acid  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Millipore ethylenediaminetretraacetic acid
    Ethylenediaminetretraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetretraacetic acid/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetretraacetic acid - by Bioz Stars, 2020-04
    95/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Dietary fiber sources and non-starch polysaccharide-degrading enzymes modify mucin expression and the immune profile of the swine ileum
    Article Snippet: Briefly, a piece of ileum of 2 to 3 cm was obtained at the time of euthanasia, washed three times in phosphate saline buffer with 1% antibiotic/antimycotic (Gibco), followed by dissociation in Hank’s balanced salt solution (Ca and Mg free, Gibco) with 30 mM ethylenediaminetretraacetic acid (Sigma Aldrich), 1mM dithiothreitol (Sigma Aldrich), and 1% penicillin/streptomycin (Gibco) at 37°C with agitation for 10–15 min, or until isolated crypts were visible. .. After the last centrifugation, the pelleted crypts were re-suspended in a small volume of DMEM/F12, mixed with Matrigel (Corning) in 1:3 ratio, and plated as beads in 24-well plates (Corning).

    Isolation:

    Article Title: Dietary fiber sources and non-starch polysaccharide-degrading enzymes modify mucin expression and the immune profile of the swine ileum
    Article Snippet: .. Briefly, a piece of ileum of 2 to 3 cm was obtained at the time of euthanasia, washed three times in phosphate saline buffer with 1% antibiotic/antimycotic (Gibco), followed by dissociation in Hank’s balanced salt solution (Ca and Mg free, Gibco) with 30 mM ethylenediaminetretraacetic acid (Sigma Aldrich), 1mM dithiothreitol (Sigma Aldrich), and 1% penicillin/streptomycin (Gibco) at 37°C with agitation for 10–15 min, or until isolated crypts were visible. .. The crypts were centrifuged at 400 x g for 5 min and washed once with DMEM/F12.

    Cell Culture:

    Article Title: Dietary fiber sources and non-starch polysaccharide-degrading enzymes modify mucin expression and the immune profile of the swine ileum
    Article Snippet: Swine enteroids were cultured from isolated crypts from adult swine ileum. .. Briefly, a piece of ileum of 2 to 3 cm was obtained at the time of euthanasia, washed three times in phosphate saline buffer with 1% antibiotic/antimycotic (Gibco), followed by dissociation in Hank’s balanced salt solution (Ca and Mg free, Gibco) with 30 mM ethylenediaminetretraacetic acid (Sigma Aldrich), 1mM dithiothreitol (Sigma Aldrich), and 1% penicillin/streptomycin (Gibco) at 37°C with agitation for 10–15 min, or until isolated crypts were visible.

    Expressing:

    Article Title: Dietary fiber sources and non-starch polysaccharide-degrading enzymes modify mucin expression and the immune profile of the swine ileum
    Article Snippet: Crypt isolation and enteroid culture Enteroids were cultured from mouse isolated crypts obtained following the protocols described by Mahe et al. [ ], with culture media formulated with advanced DMEM/F12 (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 2mM GlutaMax (Gibco), 10 mM HEPES (Gibco), 100 U/mL penicillin/100 μg/mL streptomycin (Gibco), 1× N2 (Gibco) and 1× B27 (Gibco) supplements, 100 ng/mL Noggin (Gibco), 50 ng/mL epithelial growth factor (R & D Systems, Minneapolis, MN), 5% R-spondin, 5% Wnt-3a conditioned media obtained from transformed Hek293 cells expressing R-spondin (a kind gift from Dr. Jason Spence, University of Michigan), and Wnt-3a L-cells (ATTCC CRL-2647). .. Briefly, a piece of ileum of 2 to 3 cm was obtained at the time of euthanasia, washed three times in phosphate saline buffer with 1% antibiotic/antimycotic (Gibco), followed by dissociation in Hank’s balanced salt solution (Ca and Mg free, Gibco) with 30 mM ethylenediaminetretraacetic acid (Sigma Aldrich), 1mM dithiothreitol (Sigma Aldrich), and 1% penicillin/streptomycin (Gibco) at 37°C with agitation for 10–15 min, or until isolated crypts were visible.

    Transformation Assay:

    Article Title: Dietary fiber sources and non-starch polysaccharide-degrading enzymes modify mucin expression and the immune profile of the swine ileum
    Article Snippet: Crypt isolation and enteroid culture Enteroids were cultured from mouse isolated crypts obtained following the protocols described by Mahe et al. [ ], with culture media formulated with advanced DMEM/F12 (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 2mM GlutaMax (Gibco), 10 mM HEPES (Gibco), 100 U/mL penicillin/100 μg/mL streptomycin (Gibco), 1× N2 (Gibco) and 1× B27 (Gibco) supplements, 100 ng/mL Noggin (Gibco), 50 ng/mL epithelial growth factor (R & D Systems, Minneapolis, MN), 5% R-spondin, 5% Wnt-3a conditioned media obtained from transformed Hek293 cells expressing R-spondin (a kind gift from Dr. Jason Spence, University of Michigan), and Wnt-3a L-cells (ATTCC CRL-2647). .. Briefly, a piece of ileum of 2 to 3 cm was obtained at the time of euthanasia, washed three times in phosphate saline buffer with 1% antibiotic/antimycotic (Gibco), followed by dissociation in Hank’s balanced salt solution (Ca and Mg free, Gibco) with 30 mM ethylenediaminetretraacetic acid (Sigma Aldrich), 1mM dithiothreitol (Sigma Aldrich), and 1% penicillin/streptomycin (Gibco) at 37°C with agitation for 10–15 min, or until isolated crypts were visible.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore ethylenediaminetetraacetic acid
    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with <t>ethylenediaminetetraacetic</t> acid shows absence of activity. (C)
    Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Journal: Tissue Engineering. Part A

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue

    doi: 10.1089/ten.tea.2018.0036

    Figure Lengend Snippet: Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Article Snippet: Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control.

    Techniques: Expressing, Activity Assay, RNA Sequencing Assay, Zymography, Recombinant, Inhibition