ethylenediaminetetracetic acid  (Millipore)


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    Structured Review

    Millipore ethylenediaminetetracetic acid
    Ethylenediaminetetracetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetracetic acid/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetracetic acid - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: A Biomarker to Differentiate between Primary and Cocaine-Induced Major Depression in Cocaine Use Disorder: The Role of Platelet IRAS/Nischarin (I1-Imidazoline Receptor)
    Article Snippet: Platelet Membrane Preparation Platelet samples were washed in 500 µl of 0.9% NaCl, isolated by centrifugation (3,000 × g for 15 min at 4°C) and prepared (with minor modifications) for Western blot analysis as described ( , ). .. Briefly, the pellet was sonicated in 1 ml of 50 mmol/l Tris buffer, pH 7.5, containing 1 mmol/l ethylenediaminetetracetic acid, 2 mmol/l magnesium chloride (MgCl2 ), as well as 10 µl/ml of protease and phosphatase inhibitor cocktails (P8340 and P2850; Sigma-Aldrich).

    Cytometry:

    Article Title: Monophasic and Biphasic Electrical Stimulation Induces a Precardiac Differentiation in Progenitor Cells Isolated from Human Heart
    Article Snippet: Cells from the third passage after immunoselection were detached with 5 mM ethylenediaminetetracetic acid (EDTA; Sigma-Aldrich), washed twice with phosphate-buffered saline (PBS), and incubated 20 min with PE-labeled antibodies against CD117, CD90, CD34, CD45 (Biolegend), and Sca-1 (BD Bio-sciences) and FITC-labeled CD105 and CD44 antibodies (Biolegend), following the manufacturer's instructions. .. Cells were then washed twice with ice-cold PBS and fixed in buffered 1% paraformaldehyde, 2% FBS for 15 min at 4°C, and analyzed with the FACScalibur flow cytometer (BD Bio-sciences) within 48 h.

    Electrophoresis:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. After electrophoresis, the proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Bedford, MA, USA) in a semi-dry blotting apparatus (Bio-Rad, Hercules, CA, USA).

    Incubation:

    Article Title: Monophasic and Biphasic Electrical Stimulation Induces a Precardiac Differentiation in Progenitor Cells Isolated from Human Heart
    Article Snippet: .. Cells from the third passage after immunoselection were detached with 5 mM ethylenediaminetetracetic acid (EDTA; Sigma-Aldrich), washed twice with phosphate-buffered saline (PBS), and incubated 20 min with PE-labeled antibodies against CD117, CD90, CD34, CD45 (Biolegend), and Sca-1 (BD Bio-sciences) and FITC-labeled CD105 and CD44 antibodies (Biolegend), following the manufacturer's instructions. .. Cells were then washed twice with ice-cold PBS and fixed in buffered 1% paraformaldehyde, 2% FBS for 15 min at 4°C, and analyzed with the FACScalibur flow cytometer (BD Bio-sciences) within 48 h.

    Activity Assay:

    Article Title: Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation, et al. Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation
    Article Snippet: Tibiae were subsequently decalcified in 10% ethylenediaminetetracetic acid (EDTA/PBS, pH 7.4 at 4°C; Sigma–Aldrich, Dorset, UK) for 3–4 weeks with regular changes. .. To analyze osteoclast and osteoblast numbers, sections were reacted for tartrate‐resistant acid phosphatase activity (TRAP) and stained by H & E respectively, as described previously (Erlebacher & Derynck, ).

    Expressing:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: .. Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. The protein concentrations were determined using the Bradford method (Bio-Rad, Hercules, CA, USA) with bovine serum albumin as the standard.

    Western Blot:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: .. Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. The protein concentrations were determined using the Bradford method (Bio-Rad, Hercules, CA, USA) with bovine serum albumin as the standard.

    Article Title: A Biomarker to Differentiate between Primary and Cocaine-Induced Major Depression in Cocaine Use Disorder: The Role of Platelet IRAS/Nischarin (I1-Imidazoline Receptor)
    Article Snippet: Platelet Membrane Preparation Platelet samples were washed in 500 µl of 0.9% NaCl, isolated by centrifugation (3,000 × g for 15 min at 4°C) and prepared (with minor modifications) for Western blot analysis as described ( , ). .. Briefly, the pellet was sonicated in 1 ml of 50 mmol/l Tris buffer, pH 7.5, containing 1 mmol/l ethylenediaminetetracetic acid, 2 mmol/l magnesium chloride (MgCl2 ), as well as 10 µl/ml of protease and phosphatase inhibitor cocktails (P8340 and P2850; Sigma-Aldrich).

    Electron Microscopy:

    Article Title: Characterization of the annulus fibrosus–vertebral body interface: identification of new structural features
    Article Snippet: Selected samples were fixed in formalin, decalcified in 0.5 m ethylenediaminetetracetic acid [EDTA (pH 7.4); Sigma Chemical, St Louis, MO, USA] at 4 °C and embedded in paraffin. .. To examine the mineral at the interface, other disc samples were processed undecalcified, dehydrated in graded acetone concentrations, followed by embedding in Spurr’s epoxy media (Electron Microscopy Sciences, Hatfield, PA, USA).

    Immunohistochemistry:

    Article Title: Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation, et al. Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation
    Article Snippet: Tibiae were subsequently decalcified in 10% ethylenediaminetetracetic acid (EDTA/PBS, pH 7.4 at 4°C; Sigma–Aldrich, Dorset, UK) for 3–4 weeks with regular changes. .. Sections (5 μm) were cut and used for histological and immunohistochemical analysis.

    Protease Inhibitor:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: .. Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. The protein concentrations were determined using the Bradford method (Bio-Rad, Hercules, CA, USA) with bovine serum albumin as the standard.

    Article Title: Leaf morphological and physiological adaptations of a deciduous oak (Quercus faginea Lam.) to the Mediterranean climate: a comparison with a closely related temperate species (Quercus robur L.)
    Article Snippet: .. Leaves from Q. robur and Q. faginea were ground in 500 µl of ice-cold extraction buffer containing 50 mM Bicine–NaOH (pH 8.0), 1 mM ethylenediaminetetracetic acid, 5% polyvinyl pyrrolidone, 6% polyethylene glycol (PEG4000 ), 50 mM β-mercaptoethanol, 10 mM dithiothreitol and 1% protease-inhibitor cocktail (Sigma-Aldrich Co. ..

    other:

    Article Title: A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation
    Article Snippet: Fluorescein isothiocyanate (FITC) albumin, potassium hydroxide, polyvinylpyrrolidone K-90 (PVP, MW = 360 000 Da), urea, Triton X-100, hydrochloric acid (HCl), ethanolamine, barium hydroxide (Ba(OH)2 ), ɛ-amino- n- caproic acid (EACA), β-mercaptoethanol sodium chloride (NaCl), potassium chloride (KCl), carbenicillin, dithiothreitol (DTT), ethylenediaminetetracetic acid, sodium azide (NaN3 ), phenylmethylsulfonylfluoride (PMSF), benzamidine, ammonium sulfate ((NH4 )2 SO4 ), citric acid, and disodium phosphate (Na2 HPO4) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Protein Concentration:

    Article Title: A Biomarker to Differentiate between Primary and Cocaine-Induced Major Depression in Cocaine Use Disorder: The Role of Platelet IRAS/Nischarin (I1-Imidazoline Receptor)
    Article Snippet: Briefly, the pellet was sonicated in 1 ml of 50 mmol/l Tris buffer, pH 7.5, containing 1 mmol/l ethylenediaminetetracetic acid, 2 mmol/l magnesium chloride (MgCl2 ), as well as 10 µl/ml of protease and phosphatase inhibitor cocktails (P8340 and P2850; Sigma-Aldrich). .. Bicinchoninic acid assay was used to determine the sample protein concentration, which was adjusted to 3–4 µg/µl with protease/phosphatase inhibitor-free Tris buffer.

    Sonication:

    Article Title: A Biomarker to Differentiate between Primary and Cocaine-Induced Major Depression in Cocaine Use Disorder: The Role of Platelet IRAS/Nischarin (I1-Imidazoline Receptor)
    Article Snippet: .. Briefly, the pellet was sonicated in 1 ml of 50 mmol/l Tris buffer, pH 7.5, containing 1 mmol/l ethylenediaminetetracetic acid, 2 mmol/l magnesium chloride (MgCl2 ), as well as 10 µl/ml of protease and phosphatase inhibitor cocktails (P8340 and P2850; Sigma-Aldrich). ..

    Nucleic Acid Electrophoresis:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. An aliquot of tissue extract containing 20 µg of protein was separated on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel.

    Article Title: Leaf morphological and physiological adaptations of a deciduous oak (Quercus faginea Lam.) to the Mediterranean climate: a comparison with a closely related temperate species (Quercus robur L.)
    Article Snippet: Leaves from Q. robur and Q. faginea were ground in 500 µl of ice-cold extraction buffer containing 50 mM Bicine–NaOH (pH 8.0), 1 mM ethylenediaminetetracetic acid, 5% polyvinyl pyrrolidone, 6% polyethylene glycol (PEG4000 ), 50 mM β-mercaptoethanol, 10 mM dithiothreitol and 1% protease-inhibitor cocktail (Sigma-Aldrich Co. .. The concentration of Rubisco was determined with the gel electrophoresis method ( , ) using known concentrations of purified Rubisco from wheat as a standard for calibration.

    Isolation:

    Article Title: A Biomarker to Differentiate between Primary and Cocaine-Induced Major Depression in Cocaine Use Disorder: The Role of Platelet IRAS/Nischarin (I1-Imidazoline Receptor)
    Article Snippet: Platelet Membrane Preparation Platelet samples were washed in 500 µl of 0.9% NaCl, isolated by centrifugation (3,000 × g for 15 min at 4°C) and prepared (with minor modifications) for Western blot analysis as described ( , ). .. Briefly, the pellet was sonicated in 1 ml of 50 mmol/l Tris buffer, pH 7.5, containing 1 mmol/l ethylenediaminetetracetic acid, 2 mmol/l magnesium chloride (MgCl2 ), as well as 10 µl/ml of protease and phosphatase inhibitor cocktails (P8340 and P2850; Sigma-Aldrich).

    Flow Cytometry:

    Article Title: Monophasic and Biphasic Electrical Stimulation Induces a Precardiac Differentiation in Progenitor Cells Isolated from Human Heart
    Article Snippet: Cells from the third passage after immunoselection were detached with 5 mM ethylenediaminetetracetic acid (EDTA; Sigma-Aldrich), washed twice with phosphate-buffered saline (PBS), and incubated 20 min with PE-labeled antibodies against CD117, CD90, CD34, CD45 (Biolegend), and Sca-1 (BD Bio-sciences) and FITC-labeled CD105 and CD44 antibodies (Biolegend), following the manufacturer's instructions. .. Cells were then washed twice with ice-cold PBS and fixed in buffered 1% paraformaldehyde, 2% FBS for 15 min at 4°C, and analyzed with the FACScalibur flow cytometer (BD Bio-sciences) within 48 h.

    Mouse Assay:

    Article Title: Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation, et al. Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation
    Article Snippet: 4.7 Tissue processing for histological techniques Soft tissues (liver, spleen, lung, heart, and kidney) and tibia/femurs were dissected from freshly killed male and female mice and fixed in 4% paraformaldehyde (PFA) for 24 hr at 4°C. .. Tibiae were subsequently decalcified in 10% ethylenediaminetetracetic acid (EDTA/PBS, pH 7.4 at 4°C; Sigma–Aldrich, Dorset, UK) for 3–4 weeks with regular changes.

    Purification:

    Article Title: Leaf morphological and physiological adaptations of a deciduous oak (Quercus faginea Lam.) to the Mediterranean climate: a comparison with a closely related temperate species (Quercus robur L.)
    Article Snippet: Leaves from Q. robur and Q. faginea were ground in 500 µl of ice-cold extraction buffer containing 50 mM Bicine–NaOH (pH 8.0), 1 mM ethylenediaminetetracetic acid, 5% polyvinyl pyrrolidone, 6% polyethylene glycol (PEG4000 ), 50 mM β-mercaptoethanol, 10 mM dithiothreitol and 1% protease-inhibitor cocktail (Sigma-Aldrich Co. .. The concentration of Rubisco was determined with the gel electrophoresis method ( , ) using known concentrations of purified Rubisco from wheat as a standard for calibration.

    Article Title: Glycidyl Methacrylate-Emulsion Styrene Butadiene Rubber (GMA-ESBR)/Silica Wet Masterbatch Compound
    Article Snippet: Materials for Emulsion Polymerization 1,3-Butadiene, surfactant fatty acid, and rosin soap were supplied by Kumho Petrochemical Co. and used without further purification. .. A chain transfer agent; tert-dodecyl mercaptan, an initiator; p-menthane hydroperoxide (PMH, 98%), catalysts; sodium formaldehyde sulfoxylate (SFS, 86%), ferrous sulfate (FES, 99%), and ethylenediaminetetracetic acid, and a shortstop agent; diethylhydroxylamine (98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    SDS Page:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. An aliquot of tissue extract containing 20 µg of protein was separated on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel.

    Semi Dry Blot:

    Article Title: Conjugated linoleic acid supplementation enhances insulin sensitivity and peroxisome proliferator-activated receptor gamma and glucose transporter type 4 protein expression in the skeletal muscles of rats during endurance exercise
    Article Snippet: Western blot analysis In order to analyses PPAR-γ and GLUT-4 protein expression, muscles (the SOM and EDL) were homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l ethylenediaminetetracetic acid, 2 mmol/l phenylmethylsulfonyl fluoride, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). .. After electrophoresis, the proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Bedford, MA, USA) in a semi-dry blotting apparatus (Bio-Rad, Hercules, CA, USA).

    Acid Assay:

    Article Title: A Biomarker to Differentiate between Primary and Cocaine-Induced Major Depression in Cocaine Use Disorder: The Role of Platelet IRAS/Nischarin (I1-Imidazoline Receptor)
    Article Snippet: Briefly, the pellet was sonicated in 1 ml of 50 mmol/l Tris buffer, pH 7.5, containing 1 mmol/l ethylenediaminetetracetic acid, 2 mmol/l magnesium chloride (MgCl2 ), as well as 10 µl/ml of protease and phosphatase inhibitor cocktails (P8340 and P2850; Sigma-Aldrich). .. Bicinchoninic acid assay was used to determine the sample protein concentration, which was adjusted to 3–4 µg/µl with protease/phosphatase inhibitor-free Tris buffer.

    Staining:

    Article Title: Characterization of the annulus fibrosus–vertebral body interface: identification of new structural features
    Article Snippet: Selected samples were fixed in formalin, decalcified in 0.5 m ethylenediaminetetracetic acid [EDTA (pH 7.4); Sigma Chemical, St Louis, MO, USA] at 4 °C and embedded in paraffin. .. Five-micron-thick decalcified or undecalcified sections were cut and stained with either toluidine blue to assess the presence of sulfated proteoglycans, or hematoxylin and eosin (H & E) to visualize cells at the AF–VB interface.

    Article Title: Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation, et al. Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation
    Article Snippet: Tibiae were subsequently decalcified in 10% ethylenediaminetetracetic acid (EDTA/PBS, pH 7.4 at 4°C; Sigma–Aldrich, Dorset, UK) for 3–4 weeks with regular changes. .. To analyze osteoclast and osteoblast numbers, sections were reacted for tartrate‐resistant acid phosphatase activity (TRAP) and stained by H & E respectively, as described previously (Erlebacher & Derynck, ).

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    Millipore ethylenediaminetetraacetic acid
    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with <t>ethylenediaminetetraacetic</t> acid shows absence of activity. (C)
    Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
    99/100 stars
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    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Journal: Tissue Engineering. Part A

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue

    doi: 10.1089/ten.tea.2018.0036

    Figure Lengend Snippet: Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Article Snippet: Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control.

    Techniques: Expressing, Activity Assay, RNA Sequencing Assay, Zymography, Recombinant, Inhibition