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Merck KGaA ethylenediaminetetraacetic acids
Ethylenediaminetetraacetic Acids, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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ethylenediaminetetraacetic acids - by Bioz Stars, 2020-04
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Modification:

Article Title: Development of 3D in vitro platform technology to engineer mesenchymal stem cells
Article Snippet: Then the cells were suspended in a growth medium containing high-glucose Dulbecco’s Modified Eagle Medium supplemented with 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL streptomycin. .. When the cell cultures reached 80% confluence they were harvested by trypsinization with an aqueous solution of 0.05 wt% trypsin (Gibco) and 1 mM ethylenediaminetetraacetic acids (Merck, Darmstadt, Germany) in 0.1 M PBS (pH 7.4) for 3 minutes at 37°C and reseeded in new flasks at a density of 10–15 × 103 cells/cm2 .

Isolation:

Article Title: Development of 3D in vitro platform technology to engineer mesenchymal stem cells
Article Snippet: Paragraph title: Isolation and culture of MSC ... When the cell cultures reached 80% confluence they were harvested by trypsinization with an aqueous solution of 0.05 wt% trypsin (Gibco) and 1 mM ethylenediaminetetraacetic acids (Merck, Darmstadt, Germany) in 0.1 M PBS (pH 7.4) for 3 minutes at 37°C and reseeded in new flasks at a density of 10–15 × 103 cells/cm2 .

Incubation:

Article Title: Development of 3D in vitro platform technology to engineer mesenchymal stem cells
Article Snippet: Cells in the growth medium were placed on 25-cm2 plastic flasks and incubated at 37°C in a 5% CO2 / 95% air atmosphere. .. When the cell cultures reached 80% confluence they were harvested by trypsinization with an aqueous solution of 0.05 wt% trypsin (Gibco) and 1 mM ethylenediaminetetraacetic acids (Merck, Darmstadt, Germany) in 0.1 M PBS (pH 7.4) for 3 minutes at 37°C and reseeded in new flasks at a density of 10–15 × 103 cells/cm2 .

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    Merck KGaA edta
    Dimerisation of the two human ZnT8 CTD variants. Representative ( n = 3) MST traces for dimerisation of ZnT8 CTD protein. Fluorescently labelled <t>apo‐ZnT8cR</t> (100 n m , magenta circles) was titrated (in the presence of 1 m m <t>EDTA</t> ) with unlabelled apo‐ZnT8cR protein (180 μ m –5.5 n m ), yielding a homodimerisation K d of 4.3 ± 1.3 μ m . Fluorescently labelled apo‐ZnT8cW (100 n m , teal triangles) was titrated (in the presence of 1 m m EDTA ) with unlabelled apo‐ZnT8cW protein (124 μ m –3.8 n m ), with a homodimerisation K d of 1.8 ± 0.1 μ m . There is a significant difference between the homodimerisation K d of each variant in the presence of EDTA ( n = 3, P = 0.034).
    Edta, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 95/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Merck KGaA
    Average 95 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2020-04
    95/100 stars
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    Dimerisation of the two human ZnT8 CTD variants. Representative ( n = 3) MST traces for dimerisation of ZnT8 CTD protein. Fluorescently labelled apo‐ZnT8cR (100 n m , magenta circles) was titrated (in the presence of 1 m m EDTA ) with unlabelled apo‐ZnT8cR protein (180 μ m –5.5 n m ), yielding a homodimerisation K d of 4.3 ± 1.3 μ m . Fluorescently labelled apo‐ZnT8cW (100 n m , teal triangles) was titrated (in the presence of 1 m m EDTA ) with unlabelled apo‐ZnT8cW protein (124 μ m –3.8 n m ), with a homodimerisation K d of 1.8 ± 0.1 μ m . There is a significant difference between the homodimerisation K d of each variant in the presence of EDTA ( n = 3, P = 0.034).

    Journal: The Febs Journal

    Article Title: The C‐terminal cytosolic domain of the human zinc transporter ZnT8 and its diabetes risk variant

    doi: 10.1111/febs.14402

    Figure Lengend Snippet: Dimerisation of the two human ZnT8 CTD variants. Representative ( n = 3) MST traces for dimerisation of ZnT8 CTD protein. Fluorescently labelled apo‐ZnT8cR (100 n m , magenta circles) was titrated (in the presence of 1 m m EDTA ) with unlabelled apo‐ZnT8cR protein (180 μ m –5.5 n m ), yielding a homodimerisation K d of 4.3 ± 1.3 μ m . Fluorescently labelled apo‐ZnT8cW (100 n m , teal triangles) was titrated (in the presence of 1 m m EDTA ) with unlabelled apo‐ZnT8cW protein (124 μ m –3.8 n m ), with a homodimerisation K d of 1.8 ± 0.1 μ m . There is a significant difference between the homodimerisation K d of each variant in the presence of EDTA ( n = 3, P = 0.034).

    Article Snippet: To produce ZnT8c apo‐protein, 2 mm TCEP and 1 mm EDTA were added to the 10 mL fractions from size exclusion chromatography and then concentrated to ~ 0.5 mL using a 15 mL 3 kDa MWCO centrifugal concentrator (Merck Millipore).

    Techniques: Microscale Thermophoresis, Variant Assay

    Serine proteases inhibit mucin degradation. Analysis of sputum MUC5AC and MUC5B by western blot after incubation at 37 °C over 24 h with or without of protease inhibitors. Sputum was obtained from a COPD subject 5–6 weeks after an acute exacerbation. Mucin concentration of the native control without incubation over 24 h was set to 100 %. a We used the serine protease inhibitors DFP, PMSF and TLCK, the metalloprotease inhibitors EDTA and GM6001 and the cysteine protease inhibitors leupeptin and E64. Analysis was performed in triplicate. b Incubation of COPD sputa (5–6 weeks after the onset) with A1-PI ( n = 4) and compared with control sputa

    Journal: Respiratory Research

    Article Title: Altered protease and antiprotease balance during a COPD exacerbation contributes to mucus obstruction

    doi: 10.1186/s12931-015-0247-x

    Figure Lengend Snippet: Serine proteases inhibit mucin degradation. Analysis of sputum MUC5AC and MUC5B by western blot after incubation at 37 °C over 24 h with or without of protease inhibitors. Sputum was obtained from a COPD subject 5–6 weeks after an acute exacerbation. Mucin concentration of the native control without incubation over 24 h was set to 100 %. a We used the serine protease inhibitors DFP, PMSF and TLCK, the metalloprotease inhibitors EDTA and GM6001 and the cysteine protease inhibitors leupeptin and E64. Analysis was performed in triplicate. b Incubation of COPD sputa (5–6 weeks after the onset) with A1-PI ( n = 4) and compared with control sputa

    Article Snippet: DFP (final concentration 2 mM); PMSF (final concentration 2 mM); TLCK (final concentration 10 mM); EDTA (final concentration 100 mM); E64 (final concentration 500 ng/mL) or Merck Chemical (Nottingham, UK): GM6001 (final concentration 40 μM) and leupeptin (final concentration 40 μM) were used.

    Techniques: Western Blot, Incubation, Concentration Assay

    Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% ethylenediaminetetraacetic acid (EDTA) (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA

    Journal: European Journal of Dentistry

    Article Title: Antibacterial and smear layer removal capability of oregano extract solution

    doi: 10.4103/1305-7456.149633

    Figure Lengend Snippet: Scanning electron micrographs (a) 1% oregano extract solution (OES) + 17% ethylenediaminetetraacetic acid (EDTA) (b) 2% OES + 17% EDTA (c) 5% OES + 17% EDTA

    Article Snippet: The smear layer was removed in the first seven groups with 3 ml 17% ethylenediaminetetraacetic acid (EDTA) (Merck KGaA, Darmstadt, Germany) for 1-min, followed by 3 ml 5.25% NaOCl (Wizard, Ankara, Turkey) for 1-min, and then 5 ml distillate water for 1-min as outlined by Teixeira et al .

    Techniques:

    Scanning electron micrographs of 5.25 NaOCl + 17% ethylenediaminetetraacetic acid

    Journal: European Journal of Dentistry

    Article Title: Antibacterial and smear layer removal capability of oregano extract solution

    doi: 10.4103/1305-7456.149633

    Figure Lengend Snippet: Scanning electron micrographs of 5.25 NaOCl + 17% ethylenediaminetetraacetic acid

    Article Snippet: The smear layer was removed in the first seven groups with 3 ml 17% ethylenediaminetetraacetic acid (EDTA) (Merck KGaA, Darmstadt, Germany) for 1-min, followed by 3 ml 5.25% NaOCl (Wizard, Ankara, Turkey) for 1-min, and then 5 ml distillate water for 1-min as outlined by Teixeira et al .

    Techniques:

    Analysis of sputum MUC5AC and MUC5B by Western blotting after incubation at 37°C over 6 h in the presence or absence of proteases inhibitors. Sputum was obtained from a CF subject chronically infected by P. aeruginosa . The mucin concentration of the native control without incubation was set to 100%, and results are presented relative to this. We used the serine protease inhibitors DFP, PMSF, and TLCK, the metalloprotease inhibitors EDTA and GM6001, and the cysteine protease inhibitors leupeptin and E64.

    Journal: Infection and Immunity

    Article Title: Serine Proteases Degrade Airway Mucins in Cystic Fibrosis ▿Serine Proteases Degrade Airway Mucins in Cystic Fibrosis ▿ †

    doi: 10.1128/IAI.01252-10

    Figure Lengend Snippet: Analysis of sputum MUC5AC and MUC5B by Western blotting after incubation at 37°C over 6 h in the presence or absence of proteases inhibitors. Sputum was obtained from a CF subject chronically infected by P. aeruginosa . The mucin concentration of the native control without incubation was set to 100%, and results are presented relative to this. We used the serine protease inhibitors DFP, PMSF, and TLCK, the metalloprotease inhibitors EDTA and GM6001, and the cysteine protease inhibitors leupeptin and E64.

    Article Snippet: Chemicals were purchased from Sigma (St. Louis, MO) (DFP [final concentration, 2 mM], PMSF [final concentration, 2 mM], TLCK [final concentration, 10 mM], EDTA [final concentration, 100 mM], and E64 [final concentration, 500 ng/ml]) or Merck Chemical (Nottingham, United Kingdom) (GM6001 [final concentration, 40 μM] and leupeptin [final concentration, 40 μM]).

    Techniques: Western Blot, Incubation, Infection, Concentration Assay