Structured Review

Sarstedt ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Sarstedt, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Sarstedt
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
96/100 stars

Images

Related Articles

Irradiation:

Article Title: Influence of Different Antioxidants on X-Ray Induced DNA Double-Strand Breaks (DSBs) Using γ-H2AX Immunofluorescence Microscopy in a Preliminary Study
Article Snippet: .. For pretesting γ-H2AX-foci were evaluated with EDTA and heparin containing vials in two different concentrations (1.6 mg EDTA/ml blood vs. 3.2 mg EDTA/ml blood; 9 ml S-Monovette; Sarstedt, Nümbrecht, Germany and 16 I.E. Heparin/ml blood vs. 32 I.E. Heparin/ml blood; 4.9 ml S-Monovette Lithium-Heparin 20, Sarstedt, Nümbrecht, Germany) before and after irradiation with 10 mGy. ..

Flow Cytometry:

Article Title: Transcript Analysis Reveals a Specific HOX Signature Associated with Positional Identity of Human Endothelial Cells
Article Snippet: Paragraph title: Flow mediated leucocyte adhesion ... BOECs were cultured for 48 h and then TNF-α (100 or 1000 U/ml) was added for a further 4 h. For neutrophil preparation blood was collected from healthy volunteers into EDTA (Sarstedt Ltd., UK).

In Vitro:

Article Title: Application of Piezo-Based Measuring System for Evaluation of Nucleic Acid-Based Drugs Influencing the Coagulation
Article Snippet: Paragraph title: 2.7. Simulation of ECC Using an in Vitro Rotation Model ... Immediately after the addition of oligonucleotides (0 min) and after 30 min of dynamic incubation, blood samples were collected in tubes containing ethylenediaminetetraacetic acid (1.6 mg mL−1 , EDTA, Sarstedt, Nümbrecht, Germany) for the detection of cell numbers and FPA.

Stable Transfection:

Article Title: Transcript Analysis Reveals a Specific HOX Signature Associated with Positional Identity of Human Endothelial Cells
Article Snippet: BOECs were cultured for 48 h and then TNF-α (100 or 1000 U/ml) was added for a further 4 h. For neutrophil preparation blood was collected from healthy volunteers into EDTA (Sarstedt Ltd., UK). .. Microslides containing confluent BOECs were attached to a flow system mounted on a phase contrast video microscope and neutrophils perfused at a wall shear stress of 0.1 Pa for 4 min. After bolus perfusion and a 5-min washout, the numbers of adherent neutrophils rolling, stably adherent or transmigrated were counted, and the total corrected per square millimeter per 106 cells perfused.

Isolation:

Article Title: Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study
Article Snippet: Human blood was collected from voluntary healthy donors after receiving informed consent, using S-Monovettes containing either EDTA or LiHep as anticoagulant (Sarstedt AG and Co. KG, Nümbrecht, Germany). .. Mouse blood was terminally isolated from C57BL/6 J wild type mice after isoflurane-induced anesthesia (Forene®, AbbVie AG, Baar, Switzerland) by periorbital sinus puncture and collected through Na-heparinized Micro Hematocrit-tubes (Henry Schein, Melville, NY, US) into EDTA or LiHep-coated Microvettes (Sarstedt AG and Co).

Article Title: Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study
Article Snippet: .. Mouse blood was terminally isolated from C57BL/6 J wild type mice after isoflurane-induced anesthesia (Forene®, AbbVie AG, Baar, Switzerland) by periorbital sinus puncture and collected through Na-heparinized Micro Hematocrit-tubes (Henry Schein, Melville, NY, US) into EDTA or LiHep-coated Microvettes (Sarstedt AG and Co). ..

Article Title: Effective mobilization of hematopoietic progenitor cells in G-CSF mobilization defective CD26-/- mice through AMD3100-induced disruption of the CXCL12-CXCR4 axis
Article Snippet: Paragraph title: Isolation of Cells ... Peripheral blood (PB) was obtained from euthanized mice by cardiac puncture using a 25 gauge needle and a 1.2 mL syringe containing EDTA as an anticoagulant (Sarstedt, Germany).

Incubation:

Article Title: Application of Piezo-Based Measuring System for Evaluation of Nucleic Acid-Based Drugs Influencing the Coagulation
Article Snippet: .. Immediately after the addition of oligonucleotides (0 min) and after 30 min of dynamic incubation, blood samples were collected in tubes containing ethylenediaminetetraacetic acid (1.6 mg mL−1 , EDTA, Sarstedt, Nümbrecht, Germany) for the detection of cell numbers and FPA. ..

Microscopy:

Article Title: Transcript Analysis Reveals a Specific HOX Signature Associated with Positional Identity of Human Endothelial Cells
Article Snippet: BOECs were cultured for 48 h and then TNF-α (100 or 1000 U/ml) was added for a further 4 h. For neutrophil preparation blood was collected from healthy volunteers into EDTA (Sarstedt Ltd., UK). .. Microslides containing confluent BOECs were attached to a flow system mounted on a phase contrast video microscope and neutrophils perfused at a wall shear stress of 0.1 Pa for 4 min. After bolus perfusion and a 5-min washout, the numbers of adherent neutrophils rolling, stably adherent or transmigrated were counted, and the total corrected per square millimeter per 106 cells perfused.

Mouse Assay:

Article Title: Forkhead Box O6 (FoxO6) Depletion Attenuates Hepatic Gluconeogenesis and Protects against Fat-induced Glucose Disorder in Mice *
Article Snippet: .. Mice were fasted for 16 h, and tail vein blood was collected into capillary tubes pre-coated with potassium-EDTA (Sarstedt, Nümbrecht, Germany) for determining blood glucose levels using Glucometer Elite (Bayer, IN) or plasma insulin levels using the ultrasensitive mouse insulin enzyme-linked immunosorbent assay (ALPCO, Windham, NH). ..

Article Title: Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study
Article Snippet: Human blood was collected from voluntary healthy donors after receiving informed consent, using S-Monovettes containing either EDTA or LiHep as anticoagulant (Sarstedt AG and Co. KG, Nümbrecht, Germany). .. C57BL/6 mice were maintained under pathogen-free conditions in individually ventilated cages (IVC).

Article Title: Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study
Article Snippet: .. Mouse blood was terminally isolated from C57BL/6 J wild type mice after isoflurane-induced anesthesia (Forene®, AbbVie AG, Baar, Switzerland) by periorbital sinus puncture and collected through Na-heparinized Micro Hematocrit-tubes (Henry Schein, Melville, NY, US) into EDTA or LiHep-coated Microvettes (Sarstedt AG and Co). ..

Article Title: FoxO6 Integrates Insulin Signaling With Gluconeogenesis in the Liver
Article Snippet: .. For blood chemistry, mice were fasted for 16 h, and tail vein blood samples were collected into capillary tubes precoated with potassium-EDTA (Sarstedt, Nümbrecht, Germany) for the determination of blood glucose levels using Glucometer Elite (Bayer Healthcare LLC, Mishawaka, IN) or plasma insulin levels using the ultrasensitive mouse insulin enzyme-linked immunosorbent assay (ALPCO, Windham, NH), as described previously ( , ). ..

Article Title: Effective mobilization of hematopoietic progenitor cells in G-CSF mobilization defective CD26-/- mice through AMD3100-induced disruption of the CXCL12-CXCR4 axis
Article Snippet: .. Peripheral blood (PB) was obtained from euthanized mice by cardiac puncture using a 25 gauge needle and a 1.2 mL syringe containing EDTA as an anticoagulant (Sarstedt, Germany). .. A complete blood count and blood differential analysis was done for each peripheral blood sample using a HemaVet® 950FS Cell Counter (Drew Scientific, Waterbury, CT).

Enzyme-linked Immunosorbent Assay:

Article Title: Forkhead Box O6 (FoxO6) Depletion Attenuates Hepatic Gluconeogenesis and Protects against Fat-induced Glucose Disorder in Mice *
Article Snippet: .. Mice were fasted for 16 h, and tail vein blood was collected into capillary tubes pre-coated with potassium-EDTA (Sarstedt, Nümbrecht, Germany) for determining blood glucose levels using Glucometer Elite (Bayer, IN) or plasma insulin levels using the ultrasensitive mouse insulin enzyme-linked immunosorbent assay (ALPCO, Windham, NH). ..

Article Title: FoxO6 Integrates Insulin Signaling With Gluconeogenesis in the Liver
Article Snippet: .. For blood chemistry, mice were fasted for 16 h, and tail vein blood samples were collected into capillary tubes precoated with potassium-EDTA (Sarstedt, Nümbrecht, Germany) for the determination of blood glucose levels using Glucometer Elite (Bayer Healthcare LLC, Mishawaka, IN) or plasma insulin levels using the ultrasensitive mouse insulin enzyme-linked immunosorbent assay (ALPCO, Windham, NH), as described previously ( , ). ..

Concentration Assay:

Article Title: Increased sphingosine-1-phosphate improves muscle regeneration in acutely injured mdx mice
Article Snippet: .. Peripheral blood cell analysis Blood was collected via retro-orbital blood collection using heparinized capillaries and transferred to blood collection tubes containing a final concentration of 1.6 mg/ml EDTA (SARSTEDT, Nümbrecht, Germany) for analysis. .. Analysis of whole blood was undertaken with 20 μl per sample using the Hemavet 950 FS system (Drew Scientific, Dallas, TX, USA).

Two Tailed Test:

Article Title: Effective mobilization of hematopoietic progenitor cells in G-CSF mobilization defective CD26-/- mice through AMD3100-induced disruption of the CXCL12-CXCR4 axis
Article Snippet: Peripheral blood (PB) was obtained from euthanized mice by cardiac puncture using a 25 gauge needle and a 1.2 mL syringe containing EDTA as an anticoagulant (Sarstedt, Germany). .. Comparisons of blood differentials between groups were made using two-tailed students t-test (SigmaPlot 10 / SigmaStat 3.5, Systat, Richmond, CA).

other:

Article Title: Association of anemia and hemoglobin decrease during acute stroke treatment with infarct growth and clinical outcome
Article Snippet: Laboratory parameters Blood was collected in plastic syringes containing 1.6 ml EDTA per ml of blood (Monovette®, Sarstedt, Nümbrecht, Germany).

Article Title: Plasma appearance and disappearance of an oral dose of 25-hydroxyvitamin D2 in healthy adults
Article Snippet: Sample collections and laboratory analyses Blood samples were collected into tubes containing EDTA or lithium heparin (S-Monovettes; Sarstedt Limited, Leicester, UK) and immediately placed on ice.

Cell Culture:

Article Title: Transcript Analysis Reveals a Specific HOX Signature Associated with Positional Identity of Human Endothelial Cells
Article Snippet: .. BOECs were cultured for 48 h and then TNF-α (100 or 1000 U/ml) was added for a further 4 h. For neutrophil preparation blood was collected from healthy volunteers into EDTA (Sarstedt Ltd., UK). ..

Sampling:

Article Title: Pharmacokinetics and Disposition of Rilpivirine (TMC278) Nanosuspension as a Long-Acting Injectable Antiretroviral Formulation ▿
Article Snippet: Paragraph title: Blood and tissue sampling. ... In rats, 0.3-ml blood samples on EDTA (EDTA Microvette Sarstedt) were taken in alternating fashion from the orbital venous plexus on the following occasions: (i) post-IV dosing of rilpivirine solution, on day 0 at 7 min and 1 h, 7 h, and 48 h in three rats per treatment group and at 20 min, 3 h, 24 h, and 72 h in the three other rats; and (ii) post-SC or -IM dosing of rilpivirine LA, on day 0 at 20 min and 3, 7, and 24 h and further on day 3, day 14, and day 42 at approximately 8 h in three rats per treatment group, and on day 0 at 1 h and 7 h postdose and further on days 8 and 28 at approximately 8 h in the three other rats.

Construct:

Article Title: Transcript Analysis Reveals a Specific HOX Signature Associated with Positional Identity of Human Endothelial Cells
Article Snippet: Seeding was at a density yielding confluent monolayers within 24 h. After seeding, the microslides were placed into specially constructed glass dishes and attached to a perfusion culture system pumping a small amount of medium through the microslide for 30 s once an hour as described . .. BOECs were cultured for 48 h and then TNF-α (100 or 1000 U/ml) was added for a further 4 h. For neutrophil preparation blood was collected from healthy volunteers into EDTA (Sarstedt Ltd., UK).

Injection:

Article Title: Pharmacokinetics and Disposition of Rilpivirine (TMC278) Nanosuspension as a Long-Acting Injectable Antiretroviral Formulation ▿
Article Snippet: In rats, 0.3-ml blood samples on EDTA (EDTA Microvette Sarstedt) were taken in alternating fashion from the orbital venous plexus on the following occasions: (i) post-IV dosing of rilpivirine solution, on day 0 at 7 min and 1 h, 7 h, and 48 h in three rats per treatment group and at 20 min, 3 h, 24 h, and 72 h in the three other rats; and (ii) post-SC or -IM dosing of rilpivirine LA, on day 0 at 20 min and 3, 7, and 24 h and further on day 3, day 14, and day 42 at approximately 8 h in three rats per treatment group, and on day 0 at 1 h and 7 h postdose and further on days 8 and 28 at approximately 8 h in the three other rats. .. Thymus tissue, spleen tissue, and tissue at the site of injection (i.e., muscle after IM injection and skin after SC injection) were collected at necropsy on day 56 (six animals for lymphoid tissues and three animals for the study of the injection site).

T-Test:

Article Title: Effective mobilization of hematopoietic progenitor cells in G-CSF mobilization defective CD26-/- mice through AMD3100-induced disruption of the CXCL12-CXCR4 axis
Article Snippet: Peripheral blood (PB) was obtained from euthanized mice by cardiac puncture using a 25 gauge needle and a 1.2 mL syringe containing EDTA as an anticoagulant (Sarstedt, Germany). .. Comparisons of blood differentials between groups were made using two-tailed students t-test (SigmaPlot 10 / SigmaStat 3.5, Systat, Richmond, CA).

Activated Clotting Time Assay:

Article Title: Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study
Article Snippet: Human blood was collected from voluntary healthy donors after receiving informed consent, using S-Monovettes containing either EDTA or LiHep as anticoagulant (Sarstedt AG and Co. KG, Nümbrecht, Germany). .. All animal experiments were approved by the animal experimentation review board of the canton of Bern (BE138/16) and performed in compliance with the humane care and use of laboratory animals and the Swiss animal protection act.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Sarstedt edta microvette 500k2e
    Platelet concentrations (median, 25–75th quartile) over the time in whole blood (A) and platelet-rich plasma (B) measured after sampling in <t>EDTA</t> <t>Microvette</t> and measured with Micros ES, XN-10 fluorescence and XN-10 impedance (not significant at any time for each pair of counters compared).
    Edta Microvette 500k2e, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta microvette 500k2e/product/Sarstedt
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    edta microvette 500k2e - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    91
    Sarstedt essential oil peptide edta combinations
    Growth curve analysis of ( A ) E. coli K88F4 in the presence of 1/6× minimum inhibitory concentration (MIC; 33 μg/mL) <t>nisin</t> A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), ethylenediaminetetraacetic acid <t>(EDTA)</t> 1.0% (purple circle) in combination (red diamond) and untreated control (green circle), ( B ) E. coli F3P3 in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 2% (purple circle), in combination (red diamond) and untreated control (green circle), ( C ) E. coli F2S2 in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 1% (purple circle), in combination (red diamond) and untreated control (green circle) and ( D ) E. coli F18ab in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 0.25% (purple circle), in combination (red diamond) and untreated control (green circle). ‡ denotes ETEC strains.
    Essential Oil Peptide Edta Combinations, supplied by Sarstedt, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/essential oil peptide edta combinations/product/Sarstedt
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    essential oil peptide edta combinations - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    87
    Sarstedt dipotassium edta blood tube
    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in <t>EDTA</t> blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood <t>RNA</t> tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.
    Dipotassium Edta Blood Tube, supplied by Sarstedt, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dipotassium edta blood tube/product/Sarstedt
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dipotassium edta blood tube - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    Image Search Results


    Platelet concentrations (median, 25–75th quartile) over the time in whole blood (A) and platelet-rich plasma (B) measured after sampling in EDTA Microvette and measured with Micros ES, XN-10 fluorescence and XN-10 impedance (not significant at any time for each pair of counters compared).

    Journal: BMJ Open Sport — Exercise Medicine

    Article Title: Use of platelet-rich plasma in regenerative medicine: technical tools for correct quality control

    doi: 10.1136/bmjsem-2018-000442

    Figure Lengend Snippet: Platelet concentrations (median, 25–75th quartile) over the time in whole blood (A) and platelet-rich plasma (B) measured after sampling in EDTA Microvette and measured with Micros ES, XN-10 fluorescence and XN-10 impedance (not significant at any time for each pair of counters compared).

    Article Snippet: Whole blood collected in syringes and tubes was sampled in either plastic eppendorf tube (Dominique Dutscher, Brumath, France) or EDTA microvette 500K2E (ref 20.1339.100, Sarstedt, Numbrecht, Germany).

    Techniques: Sampling, Fluorescence

    Platelet concentrations (median, 25–75th quartile) over the time in whole blood (A) and platelet-rich plasma (B) measured after sampling in EDTA Microvette and measured with Micros ES, XN-10 fluorescence and XN-10 impedance (not significant at any time for each pair of counters compared).

    Journal: BMJ Open Sport — Exercise Medicine

    Article Title: Use of platelet-rich plasma in regenerative medicine: technical tools for correct quality control

    doi: 10.1136/bmjsem-2018-000442

    Figure Lengend Snippet: Platelet concentrations (median, 25–75th quartile) over the time in whole blood (A) and platelet-rich plasma (B) measured after sampling in EDTA Microvette and measured with Micros ES, XN-10 fluorescence and XN-10 impedance (not significant at any time for each pair of counters compared).

    Article Snippet: Whole blood collected in syringes and tubes was sampled in either plastic eppendorf tube (Dominique Dutscher, Brumath, France) or EDTA microvette 500K2E (ref 20.1339.100, Sarstedt, Numbrecht, Germany).

    Techniques: Sampling, Fluorescence

    Growth curve analysis of ( A ) E. coli K88F4 in the presence of 1/6× minimum inhibitory concentration (MIC; 33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), ethylenediaminetetraacetic acid (EDTA) 1.0% (purple circle) in combination (red diamond) and untreated control (green circle), ( B ) E. coli F3P3 in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 2% (purple circle), in combination (red diamond) and untreated control (green circle), ( C ) E. coli F2S2 in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 1% (purple circle), in combination (red diamond) and untreated control (green circle) and ( D ) E. coli F18ab in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 0.25% (purple circle), in combination (red diamond) and untreated control (green circle). ‡ denotes ETEC strains.

    Journal: Antibiotics

    Article Title: Nisin in Combination with Cinnamaldehyde and EDTA to Control Growth of Escherichia coli Strains of Swine Origin

    doi: 10.3390/antibiotics6040035

    Figure Lengend Snippet: Growth curve analysis of ( A ) E. coli K88F4 in the presence of 1/6× minimum inhibitory concentration (MIC; 33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), ethylenediaminetetraacetic acid (EDTA) 1.0% (purple circle) in combination (red diamond) and untreated control (green circle), ( B ) E. coli F3P3 in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 2% (purple circle), in combination (red diamond) and untreated control (green circle), ( C ) E. coli F2S2 in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 1% (purple circle), in combination (red diamond) and untreated control (green circle) and ( D ) E. coli F18ab in the presence of 1/6× MIC (33 μg/mL) nisin A (blue inverted triangle), 1/10× MIC (125 μg/mL) cinnamaldehyde (orange triangle), EDTA 0.25% (purple circle), in combination (red diamond) and untreated control (green circle). ‡ denotes ETEC strains.

    Article Snippet: Growth Curve Experiments For growth experiments, overnight cultures were transferred (107 cfu mL−1 in a volume of 1.0 mL) into LB supplemented with the relevant concentration of nisin A and antibiotic/peptide or essential oil/peptide/EDTA combinations, and subsequently 0.2 mL was transferred to 96-well microtitre plates (Sarstedt).

    Techniques: Concentration Assay

    Kill effect of nisin in combination with cinnamaldehyde and EDTA against E. coli strains of swine origin. Kill assay performed over a defined 3 h period with E. coli K88F4 in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (1%) and untreated control (Green), E. coli F3P3 in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (2%) (Red) and untreated control (Green), E. coli F2S2 in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (1%) EDTA 2% (Red) and untreated control (Green), E. coli F18ab in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (0.25%) (Red) and untreated control (Green). Asterisks rating of * indicates statistically significant differences between groups ( p ≤ 0.05). ‡ denotes ETEC strains.

    Journal: Antibiotics

    Article Title: Nisin in Combination with Cinnamaldehyde and EDTA to Control Growth of Escherichia coli Strains of Swine Origin

    doi: 10.3390/antibiotics6040035

    Figure Lengend Snippet: Kill effect of nisin in combination with cinnamaldehyde and EDTA against E. coli strains of swine origin. Kill assay performed over a defined 3 h period with E. coli K88F4 in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (1%) and untreated control (Green), E. coli F3P3 in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (2%) (Red) and untreated control (Green), E. coli F2S2 in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (1%) EDTA 2% (Red) and untreated control (Green), E. coli F18ab in the presence of nisin (33 μg/mL) + cinnamaldehyde (125 μg/mL) + EDTA (0.25%) (Red) and untreated control (Green). Asterisks rating of * indicates statistically significant differences between groups ( p ≤ 0.05). ‡ denotes ETEC strains.

    Article Snippet: Growth Curve Experiments For growth experiments, overnight cultures were transferred (107 cfu mL−1 in a volume of 1.0 mL) into LB supplemented with the relevant concentration of nisin A and antibiotic/peptide or essential oil/peptide/EDTA combinations, and subsequently 0.2 mL was transferred to 96-well microtitre plates (Sarstedt).

    Techniques:

    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Journal: PLoS ONE

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    doi: 10.1371/journal.pone.0143321

    Figure Lengend Snippet: Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Article Snippet: Sample collection Blood from 6 healthy individuals was collected in one PAXgeneTM blood RNA tube (Becton Dickinson, 2.5 ml blood) and one dipotassium EDTA blood tube (EDTA-KE Monovette, Sarstedt, 9 ml blood) per individual.

    Techniques: Microarray, Standard Deviation, Sampling