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Nanjing Chemical Reagent Co Ltd ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Nanjing Chemical Reagent Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Nanjing Chemical Reagent Co Ltd
Average 92 stars, based on 1 article reviews
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ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
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Immunohistochemistry:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: For immunohistochemical examination, the sections were baked at 60°C for 6 h, and then dewaxed in xylene and rehydrated through a sequence of decreasing concentration of alcoholic solutions (Hubei Taikang Medical Equipment Co. Ltd.). .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min.

ALP Assay:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min. .. Subsequent to washing three times for 5 min with PBS, the sections were incubated with mouse anti-human anti-cytokeratin monoclonal antibody (dilution, 1:100; catalog no., C2562; Sigma-Aldrich, St. Louis, MO, USA), at 4°C overnight.

Purification:

Article Title: An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase
Article Snippet: Materials GGT from equine kidney (9.1 mg, 100 Units) was purchased from Sigma and used without further purification. .. GSH was purchased from Nanjing Dazhi Biotechnology Co., Ltd. CuSO4 , and EDTA were acquired from Nanjing Chemical Reagent Co., Ltd, which were both of analytical grade.

Concentration Assay:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: For immunohistochemical examination, the sections were baked at 60°C for 6 h, and then dewaxed in xylene and rehydrated through a sequence of decreasing concentration of alcoholic solutions (Hubei Taikang Medical Equipment Co. Ltd.). .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min.

Incubation:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: Endogenous peroxidase activity was quenched by 3% H2 O2 (Nanjing Chemical Reagent Co., Ltd., Nanjing, China) incubation for 10 min at room temperature. .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min.

Activity Assay:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: Endogenous peroxidase activity was quenched by 3% H2 O2 (Nanjing Chemical Reagent Co., Ltd., Nanjing, China) incubation for 10 min at room temperature. .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min.

Sequencing:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: For immunohistochemical examination, the sections were baked at 60°C for 6 h, and then dewaxed in xylene and rehydrated through a sequence of decreasing concentration of alcoholic solutions (Hubei Taikang Medical Equipment Co. Ltd.). .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min.

Staining:

Article Title: Lymphoepithelioma-like gastric carcinoma in a patient with rectal laterally spreading tumor: A case report
Article Snippet: The sections were briefly washed in distilled water prior to being stained with Harris hematoxylin and eosin (Hubei Taikang Medical Equipment Co. Ltd.) and mounted with xylene-based mounting medium (Hubei Taikang Medical Equipment Co. Ltd.). .. For antigen retrieval, tissue slides were boiled in 1 mmol/l ethylenediaminetetraacetic acid (pH 8.0; Nanjing Chemical Reagent Co., Ltd.) in an autoclave (catalog no., ALP-CL-32L; ALP Co., Ltd., Tokyo, Japan) for 10 min.

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    Nanjing Chemical Reagent Co Ltd edta
    Cyclic voltammograms obtained at the <t>GSH</t> modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that <t>EDTA</t> instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .
    Edta, supplied by Nanjing Chemical Reagent Co Ltd, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Nanjing Chemical Reagent Co Ltd
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2020-04
    85/100 stars
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    Cyclic voltammograms obtained at the GSH modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that EDTA instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .

    Journal: International Journal of Molecular Sciences

    Article Title: An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase

    doi: 10.3390/ijms13032801

    Figure Lengend Snippet: Cyclic voltammograms obtained at the GSH modified electrode in the presence of 1 μM Cu 2+ for 1 h. The GSH has been pre-incubated with 5 U/mL GGT at 37 °C (dash dot line) or 25 °C (dash line) for 30 min, or without GGT (solid line), and then incubated with a gold electrode for 16 h to form the GSH modified electrode. The dot line shows the case that EDTA instead of GGT is added in the test solution. Scan rate: 50 mV·s −1 .

    Article Snippet: GSH was purchased from Nanjing Dazhi Biotechnology Co., Ltd. CuSO4 , and EDTA were acquired from Nanjing Chemical Reagent Co., Ltd, which were both of analytical grade.

    Techniques: Modification, Incubation