ethylenediaminetetraacetic acid  (Millipore)


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    Name:
    Ethylenediaminetetraacetic acid solution
    Description:
    Ethylenediaminetetraacetic acid EDTA is an aminopolycarboxylic acid and a hexadentate ligand It chelates with metal ions to form an octahedral complex especially with cations Ethylenediaminetetraacetic acid EDTA is an anticoagulant which prevents clotting of blood It is implicated in platelet aggregation in pseudothrombocytopenia EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease It is a chelator used to remove calcium from cell washing and suspension media EDTA reduces cell clumping
    Catalog Number:
    e8008
    Price:
    None
    Applications:
    Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.
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    Structured Review

    Millipore ethylenediaminetetraacetic acid
    Ethylenediaminetetraacetic acid solution
    Ethylenediaminetetraacetic acid EDTA is an aminopolycarboxylic acid and a hexadentate ligand It chelates with metal ions to form an octahedral complex especially with cations Ethylenediaminetetraacetic acid EDTA is an anticoagulant which prevents clotting of blood It is implicated in platelet aggregation in pseudothrombocytopenia EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease It is a chelator used to remove calcium from cell washing and suspension media EDTA reduces cell clumping
    https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Millipore
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "An Interaction of Rhamnolipids with Cu2+ Ions"

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23020488

    Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.
    Figure Legend Snippet: Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Techniques Used: Concentration Assay

    Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 6.0; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.
    Figure Legend Snippet: Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 6.0; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Techniques Used: Concentration Assay

    The structures of ( a ) monorhamnolipid; ( b ) dirhamnolipid; ( c ) ethylenediaminetetraacetic acid (EDTA); and ( d ) methylglycinediacetic acid (MGDA).
    Figure Legend Snippet: The structures of ( a ) monorhamnolipid; ( b ) dirhamnolipid; ( c ) ethylenediaminetetraacetic acid (EDTA); and ( d ) methylglycinediacetic acid (MGDA).

    Techniques Used:

    2) Product Images from "Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue"

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2018.0036

    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)
    Figure Legend Snippet: Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Techniques Used: Expressing, Activity Assay, RNA Sequencing Assay, Zymography, Recombinant, Inhibition

    3) Product Images from "Combined decellularisation and dehydration improves the mechanical properties of tissue-engineered sinews"

    Article Title: Combined decellularisation and dehydration improves the mechanical properties of tissue-engineered sinews

    Journal: Journal of Tissue Engineering

    doi: 10.1177/2041731414536720

    Effect of decellularisation only on sinew constructs: (a) ultimate tensile stress and (b) maximum modulus values for sinew constructs following decellularisation alone (n = 5/6 in each group). (c) Artificial sinew construct size following treatment with EDTA and SDS as measured by digital imaging. Size refers to the total area of the construct when viewed from above (n = 6 in each group). EDTA: ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulphate. * p
    Figure Legend Snippet: Effect of decellularisation only on sinew constructs: (a) ultimate tensile stress and (b) maximum modulus values for sinew constructs following decellularisation alone (n = 5/6 in each group). (c) Artificial sinew construct size following treatment with EDTA and SDS as measured by digital imaging. Size refers to the total area of the construct when viewed from above (n = 6 in each group). EDTA: ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulphate. * p

    Techniques Used: Construct, Imaging

    4) Product Images from "C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation"

    Article Title: C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.01604

    Purified human endogenous C-reactive protein (eCRP) inhibits BzATP-induced release of interleukin-1β (IL-1β) from U937 cells. Lipopolysaccharide (LPS)-primed (1 µg/ml, 5 h) U937 cells were stimulated with BzATP (2′(3′)-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate triethylammonium salt; 100 µM) and IL-1β was measured 30 min later in cell culture supernatants. (A) eCRP dose-dependently inhibited the BzATP-induced IL-1β release, nicotine (Nic; 100 µM) served as a positive control. (B,C) Serum amyloid P (5 µg/ml), human recombinant CRP (rCRP) (5 µg/ml), or low concentrations of free phosphocholine (PC) (1 µM) did not impair IL-1β release, but a combination of rCRP and PC (1 µM) did. (D) The inhibitory effect of eCRP (CRP I; 5 µg/ml) was preserved after ultrafiltration (cutoff 10 kDa; CRP II), but abolished by ultrafiltration in the presence of ethylenediaminetetraacetic acid (1.1 mM; CRP III). PC (1 µM) reconstituted the activity of CRP III, whereas 1 µM PC alone was ineffective. (E) CRP was retained in CRP III and absent from the low molecular weight fraction (LMW). SDS-PAGE followed by staining with Brilliant Blue. (F) The effect of eCRP was reversed by nicotinic acetylcholine receptor (nAChR) antagonists mecamylamine (Mec; 100 µM), α-bungarotoxin (α-Bun; 1 µM), strychnine (Stry; 10 µM), ArIB (500 nM), and RgIA4 (200 nM). (G) In experiments using small interfering RNA (siRNA), silencing of the nAChR subunits α7, α9, and α10, but not control siRNA (con) attenuated the inhibition by eCRP. Data are presented as individual data points, bar represents median, whiskers encompass the 25th to 75th percentile. * p ≤ 0.05, different from LPS-primed cells stimulated with BzATP alone. # p ≤ 0.05, different LPS-primed cells were stimulated with BzATP and eCRP. Kruskal–Wallis followed by Mann–Whitney rank sum test.
    Figure Legend Snippet: Purified human endogenous C-reactive protein (eCRP) inhibits BzATP-induced release of interleukin-1β (IL-1β) from U937 cells. Lipopolysaccharide (LPS)-primed (1 µg/ml, 5 h) U937 cells were stimulated with BzATP (2′(3′)-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate triethylammonium salt; 100 µM) and IL-1β was measured 30 min later in cell culture supernatants. (A) eCRP dose-dependently inhibited the BzATP-induced IL-1β release, nicotine (Nic; 100 µM) served as a positive control. (B,C) Serum amyloid P (5 µg/ml), human recombinant CRP (rCRP) (5 µg/ml), or low concentrations of free phosphocholine (PC) (1 µM) did not impair IL-1β release, but a combination of rCRP and PC (1 µM) did. (D) The inhibitory effect of eCRP (CRP I; 5 µg/ml) was preserved after ultrafiltration (cutoff 10 kDa; CRP II), but abolished by ultrafiltration in the presence of ethylenediaminetetraacetic acid (1.1 mM; CRP III). PC (1 µM) reconstituted the activity of CRP III, whereas 1 µM PC alone was ineffective. (E) CRP was retained in CRP III and absent from the low molecular weight fraction (LMW). SDS-PAGE followed by staining with Brilliant Blue. (F) The effect of eCRP was reversed by nicotinic acetylcholine receptor (nAChR) antagonists mecamylamine (Mec; 100 µM), α-bungarotoxin (α-Bun; 1 µM), strychnine (Stry; 10 µM), ArIB (500 nM), and RgIA4 (200 nM). (G) In experiments using small interfering RNA (siRNA), silencing of the nAChR subunits α7, α9, and α10, but not control siRNA (con) attenuated the inhibition by eCRP. Data are presented as individual data points, bar represents median, whiskers encompass the 25th to 75th percentile. * p ≤ 0.05, different from LPS-primed cells stimulated with BzATP alone. # p ≤ 0.05, different LPS-primed cells were stimulated with BzATP and eCRP. Kruskal–Wallis followed by Mann–Whitney rank sum test.

    Techniques Used: Purification, Cell Culture, Positive Control, Recombinant, Activity Assay, Molecular Weight, SDS Page, Staining, Small Interfering RNA, Inhibition, MANN-WHITNEY

    5) Product Images from "Isolation of a Klebsiella pneumoniae Isolate of Sequence Type 258 Producing KPC-2 Carbapenemase in Korea"

    Article Title: Isolation of a Klebsiella pneumoniae Isolate of Sequence Type 258 Producing KPC-2 Carbapenemase in Korea

    Journal: The Korean Journal of Laboratory Medicine

    doi: 10.3343/kjlm.2011.31.4.298

    Results obtained with a Modified Hodge test (A) and carbapenemase inhibition test (B) for carbapenem-resistant Klebsiella pneumoniae isolate (KPN 1010). Abbreviations: MEM, meropenem; APB, aminophenylboronic acid; CLX, cloxacillin; EDTA, ethylenediaminetetraacetic acid; DPA, dipicolinic acid.
    Figure Legend Snippet: Results obtained with a Modified Hodge test (A) and carbapenemase inhibition test (B) for carbapenem-resistant Klebsiella pneumoniae isolate (KPN 1010). Abbreviations: MEM, meropenem; APB, aminophenylboronic acid; CLX, cloxacillin; EDTA, ethylenediaminetetraacetic acid; DPA, dipicolinic acid.

    Techniques Used: Modification, Inhibition

    6) Product Images from "An Interaction of Rhamnolipids with Cu2+ Ions"

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23020488

    The structures of ( a ) monorhamnolipid; ( b ) dirhamnolipid; ( c ) ethylenediaminetetraacetic acid (EDTA); and ( d ) methylglycinediacetic acid (MGDA).
    Figure Legend Snippet: The structures of ( a ) monorhamnolipid; ( b ) dirhamnolipid; ( c ) ethylenediaminetetraacetic acid (EDTA); and ( d ) methylglycinediacetic acid (MGDA).

    Techniques Used:

    Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.
    Figure Legend Snippet: Dependence of pH and pCu fixed (the negative logarithm of the concentration of Cu 2+ ions fixed by chelating agent) on the Cu 2+ to chelating agent molar ratio (n Cu :n chelating agent ) in water ( a , c , e ) and KCl solution ( b , d , f ) at pH 5.5; ( a , b ) rhamnolipids (Rh); ( c , d ) methylglycinediacetic acid (MGDA); and ( e , f ) ethylenediaminetetraacetic acid (EDTA); CMC Rh means the rhamnolipids’ critical micelle concentration.

    Techniques Used: Concentration Assay

    7) Product Images from "Combined decellularisation and dehydration improves the mechanical properties of tissue-engineered sinews"

    Article Title: Combined decellularisation and dehydration improves the mechanical properties of tissue-engineered sinews

    Journal: Journal of Tissue Engineering

    doi: 10.1177/2041731414536720

    Effect of decellularisation only on sinew constructs: (a) ultimate tensile stress and (b) maximum modulus values for sinew constructs following decellularisation alone (n = 5/6 in each group). (c) Artificial sinew construct size following treatment with EDTA and SDS as measured by digital imaging. Size refers to the total area of the construct when viewed from above (n = 6 in each group). EDTA: ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulphate. * p
    Figure Legend Snippet: Effect of decellularisation only on sinew constructs: (a) ultimate tensile stress and (b) maximum modulus values for sinew constructs following decellularisation alone (n = 5/6 in each group). (c) Artificial sinew construct size following treatment with EDTA and SDS as measured by digital imaging. Size refers to the total area of the construct when viewed from above (n = 6 in each group). EDTA: ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulphate. * p

    Techniques Used: Construct, Imaging

    8) Product Images from "Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins"

    Article Title: Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins

    Journal: Molecules

    doi: 10.3390/molecules18022183

    Quenching of 1- N -phenylnaphthylamine (NPN) fluorescence by addition of condensed tannins (0, 50 and 200 µg/mL; a ) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia ), in comparison with addition of ethylenediaminetetraacetic acid (EDTA; b ), to Escherichia coli (ATCC 25922) suspension prepared from non-exposed bacteria. Bars indicate standard error.
    Figure Legend Snippet: Quenching of 1- N -phenylnaphthylamine (NPN) fluorescence by addition of condensed tannins (0, 50 and 200 µg/mL; a ) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia ), in comparison with addition of ethylenediaminetetraacetic acid (EDTA; b ), to Escherichia coli (ATCC 25922) suspension prepared from non-exposed bacteria. Bars indicate standard error.

    Techniques Used: Fluorescence

    1- N -Phenylnaphthylamine (NPN) fluorescence of Escherichia coli (ATCC25922) pre-cultured with ethylenediaminetetraacetic acid (EDTA; 0.05µmol/mL) or condensed tannins (10 µg/mL) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia ). Bars indicate standard error.
    Figure Legend Snippet: 1- N -Phenylnaphthylamine (NPN) fluorescence of Escherichia coli (ATCC25922) pre-cultured with ethylenediaminetetraacetic acid (EDTA; 0.05µmol/mL) or condensed tannins (10 µg/mL) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia ). Bars indicate standard error.

    Techniques Used: Fluorescence, Cell Culture

    9) Product Images from "Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue"

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2018.0036

    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)
    Figure Legend Snippet: Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Techniques Used: Expressing, Activity Assay, RNA Sequencing Assay, Zymography, Recombinant, Inhibition

    10) Product Images from "C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation"

    Article Title: C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.01604

    Purified human endogenous C-reactive protein (eCRP) inhibits BzATP-induced release of interleukin-1β (IL-1β) from U937 cells. Lipopolysaccharide (LPS)-primed (1 µg/ml, 5 h) U937 cells were stimulated with BzATP (2′(3′)-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate triethylammonium salt; 100 µM) and IL-1β was measured 30 min later in cell culture supernatants. (A) eCRP dose-dependently inhibited the BzATP-induced IL-1β release, nicotine (Nic; 100 µM) served as a positive control. (B,C) Serum amyloid P (5 µg/ml), human recombinant CRP (rCRP) (5 µg/ml), or low concentrations of free phosphocholine (PC) (1 µM) did not impair IL-1β release, but a combination of rCRP and PC (1 µM) did. (D) The inhibitory effect of eCRP (CRP I; 5 µg/ml) was preserved after ultrafiltration (cutoff 10 kDa; CRP II), but abolished by ultrafiltration in the presence of ethylenediaminetetraacetic acid (1.1 mM; CRP III). PC (1 µM) reconstituted the activity of CRP III, whereas 1 µM PC alone was ineffective. (E) CRP was retained in CRP III and absent from the low molecular weight fraction (LMW). SDS-PAGE followed by staining with Brilliant Blue. (F) The effect of eCRP was reversed by nicotinic acetylcholine receptor (nAChR) antagonists mecamylamine (Mec; 100 µM), α-bungarotoxin (α-Bun; 1 µM), strychnine (Stry; 10 µM), ArIB (500 nM), and RgIA4 (200 nM). (G) In experiments using small interfering RNA (siRNA), silencing of the nAChR subunits α7, α9, and α10, but not control siRNA (con) attenuated the inhibition by eCRP. Data are presented as individual data points, bar represents median, whiskers encompass the 25th to 75th percentile. * p ≤ 0.05, different from LPS-primed cells stimulated with BzATP alone. # p ≤ 0.05, different LPS-primed cells were stimulated with BzATP and eCRP. Kruskal–Wallis followed by Mann–Whitney rank sum test.
    Figure Legend Snippet: Purified human endogenous C-reactive protein (eCRP) inhibits BzATP-induced release of interleukin-1β (IL-1β) from U937 cells. Lipopolysaccharide (LPS)-primed (1 µg/ml, 5 h) U937 cells were stimulated with BzATP (2′(3′)-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate triethylammonium salt; 100 µM) and IL-1β was measured 30 min later in cell culture supernatants. (A) eCRP dose-dependently inhibited the BzATP-induced IL-1β release, nicotine (Nic; 100 µM) served as a positive control. (B,C) Serum amyloid P (5 µg/ml), human recombinant CRP (rCRP) (5 µg/ml), or low concentrations of free phosphocholine (PC) (1 µM) did not impair IL-1β release, but a combination of rCRP and PC (1 µM) did. (D) The inhibitory effect of eCRP (CRP I; 5 µg/ml) was preserved after ultrafiltration (cutoff 10 kDa; CRP II), but abolished by ultrafiltration in the presence of ethylenediaminetetraacetic acid (1.1 mM; CRP III). PC (1 µM) reconstituted the activity of CRP III, whereas 1 µM PC alone was ineffective. (E) CRP was retained in CRP III and absent from the low molecular weight fraction (LMW). SDS-PAGE followed by staining with Brilliant Blue. (F) The effect of eCRP was reversed by nicotinic acetylcholine receptor (nAChR) antagonists mecamylamine (Mec; 100 µM), α-bungarotoxin (α-Bun; 1 µM), strychnine (Stry; 10 µM), ArIB (500 nM), and RgIA4 (200 nM). (G) In experiments using small interfering RNA (siRNA), silencing of the nAChR subunits α7, α9, and α10, but not control siRNA (con) attenuated the inhibition by eCRP. Data are presented as individual data points, bar represents median, whiskers encompass the 25th to 75th percentile. * p ≤ 0.05, different from LPS-primed cells stimulated with BzATP alone. # p ≤ 0.05, different LPS-primed cells were stimulated with BzATP and eCRP. Kruskal–Wallis followed by Mann–Whitney rank sum test.

    Techniques Used: Purification, Cell Culture, Positive Control, Recombinant, Activity Assay, Molecular Weight, SDS Page, Staining, Small Interfering RNA, Inhibition, MANN-WHITNEY

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , p K a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, p K a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Positive Control:

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue
    Article Snippet: .. Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control. ..

    Cell Culture:

    Article Title: Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
    Article Snippet: .. In addition, bacteria was also aerobically cultured in two flasks with M9 media containing 0.05 µmol/mL of ethylenediaminetetraacetic acid (EDTA; Sigma) in the same manner as above for comparison since EDTA is a commonly used membrane permeabilizer. .. The bacterial cells suspensions that were used for the OM permeability were prepared in the same manner as described in , but using phosphate buffer.

    Concentration Assay:

    Article Title: C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation
    Article Snippet: .. Endogenous CRP was dissolved at a concentration of 5 µg/ml in PBS devoid of Ca2+ and Mg2+ (Gibco) containing 1.1 mM ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich), incubated at 37°C for 15 min followed by ultrafiltration using Amicon® Ultra centrifugal filters. .. The high molecular weight fraction was diluted in PBS/EDTA, ultrafiltrated, and transferred to PBS, 5 mM Ca2+ , without EDTA by two additional ultrafiltration steps.

    Incubation:

    Article Title: C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation
    Article Snippet: .. Endogenous CRP was dissolved at a concentration of 5 µg/ml in PBS devoid of Ca2+ and Mg2+ (Gibco) containing 1.1 mM ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich), incubated at 37°C for 15 min followed by ultrafiltration using Amicon® Ultra centrifugal filters. .. The high molecular weight fraction was diluted in PBS/EDTA, ultrafiltrated, and transferred to PBS, 5 mM Ca2+ , without EDTA by two additional ultrafiltration steps.

    Inhibition:

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue
    Article Snippet: .. Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control. ..

    Construct:

    Article Title: Combined decellularisation and dehydration improves the mechanical properties of tissue-engineered sinews
    Article Snippet: .. Decellularisation After 3 weeks of culture, sinew constructs were removed from culture and decellularised using an established decellularisation protocol., Briefly, constructs were soaked in 0.1 wt% ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich) in deionised water for 4 h at room temperature. .. Next, the constructs were washed in 0.1 wt% sodium dodecyl sulphate (SDS; Sigma-Aldrich) in 0.1% EDTA for 24 h with a single change of solution at 12 h. The constructs were then washed for 1 h in phosphate-buffered saline (PBS) with a single change of PBS after 30 min. Constructs were stored in PBS until tensile testing.

    Recombinant:

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue
    Article Snippet: .. Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control. ..

    Molecular Weight:

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , p K a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, p K a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

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  • 99
    Millipore edta
    800 MHz 15 N-TROSY spectra of DAGK in <t>LMPC,</t> LMPG, DPC, and TDPC micelles at 45 °C. The samples in TDPC and LMPG contained 10 mM Bis-Tris, 2 mM magnesium chloride, 0.5 mM <t>EDTA,</t> and 10% (v/v) D 2 O, pH 6.5. The samples in DPC and LMPC contained 250 mM imidazole, 0.5 mM EDTA, and 10% (v/v) D 2 O, pH 6.5.
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore pbs edta
    Representative absorbance spectra of AMB-PEG 2 and unconjugated AMB formulations prepared in buffers with varying hydrophobicity. 20 mM AMB-PEG 2 and unconjugated AMB formulations were prepared in DMSO and resuspended in 20% and 48% ACN buffers containing 4.3% acetic acid, as well as <t>PBS-EDTA,</t> to a final concentration of 2 mM AMB. As buffer hydrophobicity increases with higher ACN concentrations, the A 348 /A 409 ratio decreases, implying that AMB-PEG is increasingly in its monomeric form. As AMB-PEG 1 and 2 have similar UV-visible absorption profiles, with identical peak height ratios in all buffers tested, data for AMB-PEG 1 is not shown. AMB-PEG formulations that have been subjected to buffer exchange to PBS-EDTA through a 10 kDa centrifugal filter have the same UV-visible absorption spectra as the initial formulation of AMB-PEG in PBS-EDTA, which contains 10% DMSO.
    Pbs Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore calpain reaction buffer
    Schematic diagram showing the relationship of <t>calpain/NF-κB/inflammation/NVU</t> damage after CCI in mice. Traumatic brain injury induces calcium overload, which, in turn, upregulates calpain. Calpain may downregulate IκB and activate NF-κB. NF-κB induces activation of TNF-α, iNOS, ICAM-1, and MMP-9. These inflammatory substances induce degradation of basal lamina and tight junction proteins, resulting in NVU disruption, leading to brain edema. MDL28170 could reverse those changes. NF-κB: Nuclear factor-κB; NVU: Neurovascular unit; CCI: Controlled cortical impact; IκB: Inhibitory-κB; TNF-α: Tumor necrosis factor-α; iNOS: Inducible nitric oxide synthase; ICAM-1: Intracellular adhesion molecule-1; MMP-9: Matrix metalloproteinase-9.
    Calpain Reaction Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    calpain reaction buffer - by Bioz Stars, 2020-07
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    92
    Millipore m edta
    Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with <t>EDTA,</t> <t>EGTA</t>
    M Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    800 MHz 15 N-TROSY spectra of DAGK in LMPC, LMPG, DPC, and TDPC micelles at 45 °C. The samples in TDPC and LMPG contained 10 mM Bis-Tris, 2 mM magnesium chloride, 0.5 mM EDTA, and 10% (v/v) D 2 O, pH 6.5. The samples in DPC and LMPC contained 250 mM imidazole, 0.5 mM EDTA, and 10% (v/v) D 2 O, pH 6.5.

    Journal: Biochemistry

    Article Title: Lyso-Phospholipid Micelles Sustain the Stability and Catalytic Activity of Diacylglycerol Kinase in the Absence of Lipids †

    doi: 10.1021/bi100575s

    Figure Lengend Snippet: 800 MHz 15 N-TROSY spectra of DAGK in LMPC, LMPG, DPC, and TDPC micelles at 45 °C. The samples in TDPC and LMPG contained 10 mM Bis-Tris, 2 mM magnesium chloride, 0.5 mM EDTA, and 10% (v/v) D 2 O, pH 6.5. The samples in DPC and LMPC contained 250 mM imidazole, 0.5 mM EDTA, and 10% (v/v) D 2 O, pH 6.5.

    Article Snippet: When the protein was purified into LMPC and DPC, D2 O and EDTA were added to 10% and 0.5 mM, respectively, and the sample was concentrated using centrifugal ultrafiltration (Millipore Ultracel, 10 ml, 10kDa cutoff) and the sample was transferred to an NMR tube.

    Techniques:

    Representative absorbance spectra of AMB-PEG 2 and unconjugated AMB formulations prepared in buffers with varying hydrophobicity. 20 mM AMB-PEG 2 and unconjugated AMB formulations were prepared in DMSO and resuspended in 20% and 48% ACN buffers containing 4.3% acetic acid, as well as PBS-EDTA, to a final concentration of 2 mM AMB. As buffer hydrophobicity increases with higher ACN concentrations, the A 348 /A 409 ratio decreases, implying that AMB-PEG is increasingly in its monomeric form. As AMB-PEG 1 and 2 have similar UV-visible absorption profiles, with identical peak height ratios in all buffers tested, data for AMB-PEG 1 is not shown. AMB-PEG formulations that have been subjected to buffer exchange to PBS-EDTA through a 10 kDa centrifugal filter have the same UV-visible absorption spectra as the initial formulation of AMB-PEG in PBS-EDTA, which contains 10% DMSO.

    Journal: PLoS ONE

    Article Title: Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy

    doi: 10.1371/journal.pone.0152112

    Figure Lengend Snippet: Representative absorbance spectra of AMB-PEG 2 and unconjugated AMB formulations prepared in buffers with varying hydrophobicity. 20 mM AMB-PEG 2 and unconjugated AMB formulations were prepared in DMSO and resuspended in 20% and 48% ACN buffers containing 4.3% acetic acid, as well as PBS-EDTA, to a final concentration of 2 mM AMB. As buffer hydrophobicity increases with higher ACN concentrations, the A 348 /A 409 ratio decreases, implying that AMB-PEG is increasingly in its monomeric form. As AMB-PEG 1 and 2 have similar UV-visible absorption profiles, with identical peak height ratios in all buffers tested, data for AMB-PEG 1 is not shown. AMB-PEG formulations that have been subjected to buffer exchange to PBS-EDTA through a 10 kDa centrifugal filter have the same UV-visible absorption spectra as the initial formulation of AMB-PEG in PBS-EDTA, which contains 10% DMSO.

    Article Snippet: AMB-PEG Particle Size Determination Using DLS 2 mM solutions of AMB-PEG were prepared in PBS-EDTA, and diafiltration carried out with PBS-EDTA using 10 kDa centrifugal filters (Millipore).

    Techniques: Concentration Assay, Buffer Exchange

    (A) Reverse phase chromatogram of AMB-PEG and unconjugated AMB, with eluted peaks detected at 406 nm. 50 μL of AMB-PEG 1 and 2, and 10 μL of unconjugated AMB dispersed in PBS-EDTA and 48% ACN respectively were injected into a C18 reverse phase column and eluted isocratically in a 48% ACN buffer at a flow rate of 0.5 ml/min for 40 minutes. Peaks were detected at 406 nm. The AMB-PEG conjugate has a shorter retention time, implying that it is more hydrophilic. From the AMB-PEG samples, AMB-PEG conjugate and free AMB (based on the retention time of unconjugated AMB) fractions were collected for further analysis via size exclusion chromatography. (B) Size exclusion chromatogram of AMB-PEG 2 and unconjugated AMB, as well as the relevant fractions collected from RPC, in a 20% ACN mobile phase. 20 mM of AMB-PEG 2 formulations and unconjugated AMB in DMSO were prepared and resuspended at 2 mM in a 20% ACN buffer. 50 μL of these samples (unconjugated AMB diluted tenfold prior to analysis) and the peak fractions collected previously from RPC were passed through a size exclusion column and eluted peaks were detected at 406 nm. Unconjugated AMB was eluted later compared to the AMB-PEG conjugate, implying that it has a smaller hydrodynamic volume compared to AMB-PEG in 20% ACN. The previously collected AMB-PEG conjugate and free AMB peak fractions had similar retention times as the AMB-PEG 2 formulation and unconjugated AMB samples respectively, thus verifying their respective peak identities. AMB-PEG 1 and 2 have identical elution profiles. (C) Size exclusion chromatogram of AMB-PEG and unconjugated AMB dispersed in PBS-EDTA. 3 μL of 2 mM AMB-PEG formulations that have been retained by 10 kDa centrifugal filters (Millipore) and 50 μL of the supernatant obtained from unconjugated AMB were analysed using a Superdex 75 size exclusion column and eluted peaks detected at 406 nm. In a PBS-EDTA mobile phase, unconjugated AMB has a shorter retention time of 20 minutes compared to AMB-PEG at 40 minutes, implying that AMB-PEG has a smaller hydrodynamic volume under these experimental conditions.

    Journal: PLoS ONE

    Article Title: Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy

    doi: 10.1371/journal.pone.0152112

    Figure Lengend Snippet: (A) Reverse phase chromatogram of AMB-PEG and unconjugated AMB, with eluted peaks detected at 406 nm. 50 μL of AMB-PEG 1 and 2, and 10 μL of unconjugated AMB dispersed in PBS-EDTA and 48% ACN respectively were injected into a C18 reverse phase column and eluted isocratically in a 48% ACN buffer at a flow rate of 0.5 ml/min for 40 minutes. Peaks were detected at 406 nm. The AMB-PEG conjugate has a shorter retention time, implying that it is more hydrophilic. From the AMB-PEG samples, AMB-PEG conjugate and free AMB (based on the retention time of unconjugated AMB) fractions were collected for further analysis via size exclusion chromatography. (B) Size exclusion chromatogram of AMB-PEG 2 and unconjugated AMB, as well as the relevant fractions collected from RPC, in a 20% ACN mobile phase. 20 mM of AMB-PEG 2 formulations and unconjugated AMB in DMSO were prepared and resuspended at 2 mM in a 20% ACN buffer. 50 μL of these samples (unconjugated AMB diluted tenfold prior to analysis) and the peak fractions collected previously from RPC were passed through a size exclusion column and eluted peaks were detected at 406 nm. Unconjugated AMB was eluted later compared to the AMB-PEG conjugate, implying that it has a smaller hydrodynamic volume compared to AMB-PEG in 20% ACN. The previously collected AMB-PEG conjugate and free AMB peak fractions had similar retention times as the AMB-PEG 2 formulation and unconjugated AMB samples respectively, thus verifying their respective peak identities. AMB-PEG 1 and 2 have identical elution profiles. (C) Size exclusion chromatogram of AMB-PEG and unconjugated AMB dispersed in PBS-EDTA. 3 μL of 2 mM AMB-PEG formulations that have been retained by 10 kDa centrifugal filters (Millipore) and 50 μL of the supernatant obtained from unconjugated AMB were analysed using a Superdex 75 size exclusion column and eluted peaks detected at 406 nm. In a PBS-EDTA mobile phase, unconjugated AMB has a shorter retention time of 20 minutes compared to AMB-PEG at 40 minutes, implying that AMB-PEG has a smaller hydrodynamic volume under these experimental conditions.

    Article Snippet: AMB-PEG Particle Size Determination Using DLS 2 mM solutions of AMB-PEG were prepared in PBS-EDTA, and diafiltration carried out with PBS-EDTA using 10 kDa centrifugal filters (Millipore).

    Techniques: Injection, Flow Cytometry, Size-exclusion Chromatography

    (A) AMB-PEG reaction scheme. The NHS ester on MS(PEG) 4 reacts with the primary amine (–NH2) group on AMB at a 1:1 molar ratio, generating conjugated AMB-PEG via amide bond formation. (B) Solubility of AMB-PEG at varying AMB:PEG molar ratios. AMB-PEG mixtures and unreacted AMB were prepared in DMSO, incubated for 2 hours and then (i) dispersed to a final concentration of 2 mM in PBS-EDTA, containing 10% DMSO by volume. (ii) The mixtures were then centrifuged to facilitate the observation of insoluble precipitates. Higher molar ratios yielded a suspension of yellow particles that precipitated upon centrifugation, similar to what was observed with unconjugated AMB.

    Journal: PLoS ONE

    Article Title: Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy

    doi: 10.1371/journal.pone.0152112

    Figure Lengend Snippet: (A) AMB-PEG reaction scheme. The NHS ester on MS(PEG) 4 reacts with the primary amine (–NH2) group on AMB at a 1:1 molar ratio, generating conjugated AMB-PEG via amide bond formation. (B) Solubility of AMB-PEG at varying AMB:PEG molar ratios. AMB-PEG mixtures and unreacted AMB were prepared in DMSO, incubated for 2 hours and then (i) dispersed to a final concentration of 2 mM in PBS-EDTA, containing 10% DMSO by volume. (ii) The mixtures were then centrifuged to facilitate the observation of insoluble precipitates. Higher molar ratios yielded a suspension of yellow particles that precipitated upon centrifugation, similar to what was observed with unconjugated AMB.

    Article Snippet: AMB-PEG Particle Size Determination Using DLS 2 mM solutions of AMB-PEG were prepared in PBS-EDTA, and diafiltration carried out with PBS-EDTA using 10 kDa centrifugal filters (Millipore).

    Techniques: Mass Spectrometry, Solubility, Incubation, Concentration Assay, Centrifugation

    Aqueous solubility of AMB-PEG 1 and 2. Increasing amounts of AMB-PEG formulations were added to PBS-EDTA, and post-centrifugation, the concentration of soluble AMB-PEG in the supernatants quantified based on their absorbance at 365 nm. Error bars denote the standard deviation between 3 independent formulations. Dotted line represents the theoretical condition where AMB formulation is completely soluble (i.e. concentration of total AMB = concentration of soluble AMB).

    Journal: PLoS ONE

    Article Title: Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy

    doi: 10.1371/journal.pone.0152112

    Figure Lengend Snippet: Aqueous solubility of AMB-PEG 1 and 2. Increasing amounts of AMB-PEG formulations were added to PBS-EDTA, and post-centrifugation, the concentration of soluble AMB-PEG in the supernatants quantified based on their absorbance at 365 nm. Error bars denote the standard deviation between 3 independent formulations. Dotted line represents the theoretical condition where AMB formulation is completely soluble (i.e. concentration of total AMB = concentration of soluble AMB).

    Article Snippet: AMB-PEG Particle Size Determination Using DLS 2 mM solutions of AMB-PEG were prepared in PBS-EDTA, and diafiltration carried out with PBS-EDTA using 10 kDa centrifugal filters (Millipore).

    Techniques: Solubility, Centrifugation, Concentration Assay, Standard Deviation

    Schematic diagram showing the relationship of calpain/NF-κB/inflammation/NVU damage after CCI in mice. Traumatic brain injury induces calcium overload, which, in turn, upregulates calpain. Calpain may downregulate IκB and activate NF-κB. NF-κB induces activation of TNF-α, iNOS, ICAM-1, and MMP-9. These inflammatory substances induce degradation of basal lamina and tight junction proteins, resulting in NVU disruption, leading to brain edema. MDL28170 could reverse those changes. NF-κB: Nuclear factor-κB; NVU: Neurovascular unit; CCI: Controlled cortical impact; IκB: Inhibitory-κB; TNF-α: Tumor necrosis factor-α; iNOS: Inducible nitric oxide synthase; ICAM-1: Intracellular adhesion molecule-1; MMP-9: Matrix metalloproteinase-9.

    Journal: Chinese Medical Journal

    Article Title: Protective Effects of Calpain Inhibition on Neurovascular Unit Injury through Downregulating Nuclear Factor-κB-related Inflammation during Traumatic Brain Injury in Mice

    doi: 10.4103/0366-6999.198001

    Figure Lengend Snippet: Schematic diagram showing the relationship of calpain/NF-κB/inflammation/NVU damage after CCI in mice. Traumatic brain injury induces calcium overload, which, in turn, upregulates calpain. Calpain may downregulate IκB and activate NF-κB. NF-κB induces activation of TNF-α, iNOS, ICAM-1, and MMP-9. These inflammatory substances induce degradation of basal lamina and tight junction proteins, resulting in NVU disruption, leading to brain edema. MDL28170 could reverse those changes. NF-κB: Nuclear factor-κB; NVU: Neurovascular unit; CCI: Controlled cortical impact; IκB: Inhibitory-κB; TNF-α: Tumor necrosis factor-α; iNOS: Inducible nitric oxide synthase; ICAM-1: Intracellular adhesion molecule-1; MMP-9: Matrix metalloproteinase-9.

    Article Snippet: [ ] In brief, cytosolic and mitochondrial proteins (30 μg) were incubated with calpain reaction buffer (20 mmol/L HEPES, 1 mmol/L EDTA, 50 mmol/L NaCl, and 0.1% (v/v) 2-mercaptoethanol, containing 10 μmol/L calpain I fluorescent substrate [Calbiochem Co., La Jolla, CA, USA], pH 7.6).

    Techniques: Mouse Assay, Activation Assay

    MDL28170 treatment suppresses the calpain activity in the cytosolic and mitochondrial fractions and upregulates the expression of calpastatin in the cytosolic fractions. (a and b) The bar graphs reflect the calpain activity in the cytosolic fractions and mitochondrial fractions at 6 h and 24 h. (c) Representative Western blots of calpastatin and β-actin from each group; (d) the results were quantified and are shown as the mean ± SD. * P

    Journal: Chinese Medical Journal

    Article Title: Protective Effects of Calpain Inhibition on Neurovascular Unit Injury through Downregulating Nuclear Factor-κB-related Inflammation during Traumatic Brain Injury in Mice

    doi: 10.4103/0366-6999.198001

    Figure Lengend Snippet: MDL28170 treatment suppresses the calpain activity in the cytosolic and mitochondrial fractions and upregulates the expression of calpastatin in the cytosolic fractions. (a and b) The bar graphs reflect the calpain activity in the cytosolic fractions and mitochondrial fractions at 6 h and 24 h. (c) Representative Western blots of calpastatin and β-actin from each group; (d) the results were quantified and are shown as the mean ± SD. * P

    Article Snippet: [ ] In brief, cytosolic and mitochondrial proteins (30 μg) were incubated with calpain reaction buffer (20 mmol/L HEPES, 1 mmol/L EDTA, 50 mmol/L NaCl, and 0.1% (v/v) 2-mercaptoethanol, containing 10 μmol/L calpain I fluorescent substrate [Calbiochem Co., La Jolla, CA, USA], pH 7.6).

    Techniques: Activity Assay, Expressing, Western Blot

    Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with EDTA, EGTA

    Journal: Immunology

    Article Title: Natural lysophospholipids reduce Mycobacterium tuberculosis-induced cytotoxicity and induce anti-mycobacterial activity by a phagolysosome maturation-dependent mechanism in A549 type II alveolar epithelial cells

    doi: 10.1111/j.1365-2567.2009.03145.x

    Figure Lengend Snippet: Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with EDTA, EGTA

    Article Snippet: In several experiments, 2 m m EDTA or 3 m m EGTA (Calbiochem) were added 15 min before the addition of LPA or S1P, whereas 20 μ m 1,2-bis(2-aminophenoxy)ethane-N,N,N1,N1-tetraacetic acid tetraicis (acetoxymethyl ester) (BAPTA-AM) (Sigma-Aldrich, Milan, Italy) was added 30 min before stimulation with LPA or S1P.

    Techniques: Activation Assay