Structured Review

Dojindo Labs ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Dojindo Labs
Average 93 stars, based on 4 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
93/100 stars

Images

Related Articles

Centrifugation:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)]. .. Extracts of 293T cells were prepared with 1× RIPA buffer and were cleared of debris by centrifugation at 20,000 g for 15 min at 4°C.

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: Following centrifugation at 16500 g for 25 min, 75 μl of the sample supernatant was diluted with 25 μl of PBS and then reduced with 10 μl of 100 g/l tris(2-carboxyethyl)phosphine for 30 min at room temperature (22 °C). .. For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5).

Cytometry:

Article Title: Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment
Article Snippet: The platelet preparation was incubated for 30 min at 37 °C with PBS containing 0.1% bovine serum albumin (Roche Diagnostics, Basel, Switzerland); 2 mM EDTA (Dojindo, Kumamoto, Japan); 0.02 unit/ml apyrase (New England BioLabs, Hertfordshire, UK); and 0.5 μM prostacyclin after washing with the same buffer. .. The prepared platelet containing fraction was analysed by flow cytometry using Aria II (Becton Dickinson) after staining with anti-CD41 FITC (eBioscience, San Diego, CA, US) and anti-CD62P APC (eBioscience).

Incubation:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: The lysates were centrifuged at 20,000 g for 15 min at 4°C, and the resulting supernatants were incubated with Dynabeads Protein G (Life Technologies) conjugated with the antibodies to FLAG. .. For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)].

Article Title: Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment
Article Snippet: .. The platelet preparation was incubated for 30 min at 37 °C with PBS containing 0.1% bovine serum albumin (Roche Diagnostics, Basel, Switzerland); 2 mM EDTA (Dojindo, Kumamoto, Japan); 0.02 unit/ml apyrase (New England BioLabs, Hertfordshire, UK); and 0.5 μM prostacyclin after washing with the same buffer. .. The prepared platelet containing fraction was analysed by flow cytometry using Aria II (Becton Dickinson) after staining with anti-CD41 FITC (eBioscience, San Diego, CA, US) and anti-CD62P APC (eBioscience).

Mass Spectrometry:

Article Title: A methodological consideration for blood lead concentrations obtained from the earlobe in Japanese adults occupationally unexposed to lead
Article Snippet: BPb concentrations of the paired samples were determined by inductively coupled plasma mass spectrometry (Agilent 7900 ICP-MS; Agilent Technologies Japan, Tokyo, Japan) using thallium (Wako Pure Chemical Industries, Ltd., Osaka, Japan) as the internal standard. .. Whole blood was diluted 1:20 with an alkaline diluent containing 2% butanol (RoHS compliant; Wako Pure Chemical Industries Ltd., Osaka, Japan), 0.05% polyoxyethylene(10) octylphenyl ether (Practical grade; Wako Pure Chemical Industries, Ltd., Osaka, Japan), 0.05% EDTA (Dojindo Laboratories, Kumamoto, Japan), and 0.1% tetramethylammonium hydroxide (Super special grade; Wako Pure Chemical Industries, Ltd., Osaka, Japan).

Modification:

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: Determination of tissue cysteine levels was performed by modification of a previously described method [ ]. .. For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5).

Western Blot:

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: Frozen livers were homogenized in 4 vol. of the same lysis buffer as used for the Western-blot analysis except that N -ethylmaleimide was not included. .. For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5).

High Performance Liquid Chromatography:

Article Title: Predator Exposure/Psychosocial Stress Animal Model of Post-Traumatic Stress Disorder Modulates Neurotransmitters in the Rat Hippocampus and Prefrontal Cortex
Article Snippet: Paragraph title: HPLC – mobile phase ... EDTA (Dojindo Laboratories, USA, mol. wt.

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: Paragraph title: Analysis of tissue cysteine, MEA and hypotaurine content by HPLC ... For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5).

Article Title: Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes
Article Snippet: .. The reagents were obtained from the following sources: calcium ionophore II ( N , N , N ′, N ′-tetracyclohexyl-3-oxapentanediamide) and bis(2-ethylhexyl) sebacate were from Fluka (Buchs, Switzerland); potassium tetrakis( p -chlorophenyl)borate (KTpClPB), sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate (NaHFPB), TPP chloride (TPPCl), EDTA, and morpholinepropanesulfonic acid (MOPS) were from Dojindo Laboratories (Kumamoto, Japan); polyvinyl chloride (PVC; degree of polymerization, 1,020) was from Nacalai Tesque (Kyoto, Japan); methoctramine tetrahydrochloride, 1-naphthylacetylspermine trihydrochloride, spermine tetrahydrochloride, spermidine trihydrochloride, benzylamine hydrochloride, 1,2-bis(2-aminophenoxy)ethane- N,N,N ′ N ′-tetraacetic acid (BAPTA), choline chloride, and melittin (from bee venom, approximately 85% by high-performance liquid chromatography) were from Sigma (St. Louis, Mo); N -benzylethylenediamine and 4- N -benzylspermidine were from Aldrich (Milwaukee, Wis.); PMBN was from Boehringer Mannheim (Mannheim, Germany); methylamine hydrochloride and ethylenediamine dihydrochloride were from Tokyo Kasei (Tokyo, Japan); and Tris was from Wako (Osaka, Japan). ..

Flow Cytometry:

Article Title: Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment
Article Snippet: The platelet preparation was incubated for 30 min at 37 °C with PBS containing 0.1% bovine serum albumin (Roche Diagnostics, Basel, Switzerland); 2 mM EDTA (Dojindo, Kumamoto, Japan); 0.02 unit/ml apyrase (New England BioLabs, Hertfordshire, UK); and 0.5 μM prostacyclin after washing with the same buffer. .. The prepared platelet containing fraction was analysed by flow cytometry using Aria II (Becton Dickinson) after staining with anti-CD41 FITC (eBioscience, San Diego, CA, US) and anti-CD62P APC (eBioscience).

Article Title: An ultrasensitive enzymatic method for measuring mevalonic acid in serum
Article Snippet: HEPES, MES, and EDTA were from Dojindo Laboratories (Kumamoto, Japan). .. Q Sepharose big beads, Phenyl Sepharose fast flow, Q Sepharose fast flow, DEAE-Sepharose fast flow, and Blue Sepharose fast flow were from GE Health Care (Tokyo, Japan).

Ligation:

Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions
Article Snippet: .. Special agents The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), EGTA (Dojindo), dipyridyl (Sigma), CAS (Chrome Azurol S, Sigma), E. coli S17-1 and pK18mobSacB (kindly provided by Dr. F. Taguchi, Okayama University), Instagene matrix (Bio-Rad, Hercules, CA), KOD-Plus (Toyobo, Osaka, Japan), Wizard SV Gel and PCR cleanup system (Promeg, Madison, WI), Ligation High (Toyobo), Protein Assay Rapid Kit (Wako), [14 C] isoleucine (Moravek Biochemicals, Inc, Brea, CA), [14 C]uracil (Moravek Biochemicals, Inc.), α-defensin-1 (Peptide institute, Inc, Osaka, Japan.), cathepsin G (Sigma), vancomycin (Wako), clarithromycin (Taisho-Toyama Pharmaceutical Co., Tokyo), rifampin (Daiichi Sankyo Co., Tokyo), and ethambutol (Sigma), gatifloxacin (Wako), FLUOS (Sigma), LB medium (Invitrogen, San Diego, CA), M9 medium (prepared by our laboratory), Heart infusion agar (Eiken Chemical Co., Tokyo, Japan), Middlebrook 7H9 medium (Becton Dickinson, Cockeysville, MD), and Middlebrook 7H11 medium (Becton Dickinson). .. Mice Twelve-week-old male BALB/c mice purchased from Japan Clea Co., Osaka, Japan, were used.

Protease Inhibitor:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: .. For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)]. .. The homogenate was then mixed with an equal volume of 2× radioimmunoprecipitation assay (RIPA) buffer.

other:

Article Title: Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions
Article Snippet: Ethylenediaminetetraacetic acid

Polymerase Chain Reaction:

Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions
Article Snippet: .. Special agents The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), EGTA (Dojindo), dipyridyl (Sigma), CAS (Chrome Azurol S, Sigma), E. coli S17-1 and pK18mobSacB (kindly provided by Dr. F. Taguchi, Okayama University), Instagene matrix (Bio-Rad, Hercules, CA), KOD-Plus (Toyobo, Osaka, Japan), Wizard SV Gel and PCR cleanup system (Promeg, Madison, WI), Ligation High (Toyobo), Protein Assay Rapid Kit (Wako), [14 C] isoleucine (Moravek Biochemicals, Inc, Brea, CA), [14 C]uracil (Moravek Biochemicals, Inc.), α-defensin-1 (Peptide institute, Inc, Osaka, Japan.), cathepsin G (Sigma), vancomycin (Wako), clarithromycin (Taisho-Toyama Pharmaceutical Co., Tokyo), rifampin (Daiichi Sankyo Co., Tokyo), and ethambutol (Sigma), gatifloxacin (Wako), FLUOS (Sigma), LB medium (Invitrogen, San Diego, CA), M9 medium (prepared by our laboratory), Heart infusion agar (Eiken Chemical Co., Tokyo, Japan), Middlebrook 7H9 medium (Becton Dickinson, Cockeysville, MD), and Middlebrook 7H11 medium (Becton Dickinson). .. Mice Twelve-week-old male BALB/c mice purchased from Japan Clea Co., Osaka, Japan, were used.

Injection:

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5). .. A total of 80 μl of sample was injected on to the column by an automated sample injector (WISP 717 Plus; Waters).

Nucleic Acid Electrophoresis:

Article Title: Single Molecular Observation of Self-Regulated Kinesin Motility †
Article Snippet: PIPES, EDTA, and EGTA were obtained from Dojindo Laboratories (Kumamoto, Japan). .. SDS−polyacrylamide gel electrophoresis (SDS−PAGE) was performed using a 15% separating gel.

Microelectrode Array:

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: Paragraph title: Analysis of tissue cysteine, MEA and hypotaurine content by HPLC ... For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5).

Microscopy:

Article Title: Transplantation of Olfactory Stem Cells with Biodegradable Hydrogel Accelerates Facial Nerve Regeneration After Crush Injury
Article Snippet: Following perfusion, the head was removed, decalcified in ethylenediaminetetraacetic acid (EDTA, Dojindo Molecular Technologies, Inc.), and embedded in paraffin. .. At 1 mm distal to the compression site, the structure of the facial nerve was evaluated via microscopy (Olympus Co., Ltd., Tokyo, Japan) with a CCD camera (Nikon, Co., Ltd., Tokyo, Japan).

Mouse Assay:

Article Title: Transplantation of Olfactory Stem Cells with Biodegradable Hydrogel Accelerates Facial Nerve Regeneration After Crush Injury
Article Snippet: In short, mice were transcardially perfused with 4% PFA in PBS under deep anesthesia with ketamine (250 mg/kg) and xylazine (25 mg/kg). .. Following perfusion, the head was removed, decalcified in ethylenediaminetetraacetic acid (EDTA, Dojindo Molecular Technologies, Inc.), and embedded in paraffin.

Lysis:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: For immunoprecipitation, 293T cells were washed with PBS and lysed for 10 min at 4°C in NP-40 lysis buffer [0.5% Nonidet P-40, 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10% glycerol] and a protease inhibitor cocktail [10 μg/ml aprotinin (Sigma), 10 μg/ml leupeptin (Peptide institute) and 1 mM PMSF (Wako)]. .. For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)].

Article Title: Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26 S proteasome system in the living rat
Article Snippet: Frozen livers were homogenized in 4 vol. of the same lysis buffer as used for the Western-blot analysis except that N -ethylmaleimide was not included. .. For thiol derivatization, 50 μl of sample supernatant was added to an autosampler vial containing 10 μl of 1.55 mM NaOH, 125 μl of 125 mM borate buffer containing 4 mM EDTA (pH 9.5) and 50 μl of 1 g/l SBD-F (ammonium 4-fluoro-7-sulphobenzofurazan; Dojindo Laboratories, Kumamato, Japan) dissolved in 125 mM borate buffer with 4 mM EDTA (pH 9.5).

Purification:

Article Title: A methodological consideration for blood lead concentrations obtained from the earlobe in Japanese adults occupationally unexposed to lead
Article Snippet: Whole blood was diluted 1:20 with an alkaline diluent containing 2% butanol (RoHS compliant; Wako Pure Chemical Industries Ltd., Osaka, Japan), 0.05% polyoxyethylene(10) octylphenyl ether (Practical grade; Wako Pure Chemical Industries, Ltd., Osaka, Japan), 0.05% EDTA (Dojindo Laboratories, Kumamoto, Japan), and 0.1% tetramethylammonium hydroxide (Super special grade; Wako Pure Chemical Industries, Ltd., Osaka, Japan). .. Milli-Q water used in the experiment ( > 18.2 MΩ) was deionized and purified using a Milli-Q system (Merck KGaA, Darmstadt, Germany).

SDS Page:

Article Title: Single Molecular Observation of Self-Regulated Kinesin Motility †
Article Snippet: PIPES, EDTA, and EGTA were obtained from Dojindo Laboratories (Kumamoto, Japan). .. SDS−polyacrylamide gel electrophoresis (SDS−PAGE) was performed using a 15% separating gel.

Radio Immunoprecipitation:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)]. .. The homogenate was then mixed with an equal volume of 2× radioimmunoprecipitation assay (RIPA) buffer.

Homogenization:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: .. For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)]. .. The homogenate was then mixed with an equal volume of 2× radioimmunoprecipitation assay (RIPA) buffer.

Activation Assay:

Article Title: Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment
Article Snippet: Paragraph title: Platelet activation assay ... The platelet preparation was incubated for 30 min at 37 °C with PBS containing 0.1% bovine serum albumin (Roche Diagnostics, Basel, Switzerland); 2 mM EDTA (Dojindo, Kumamoto, Japan); 0.02 unit/ml apyrase (New England BioLabs, Hertfordshire, UK); and 0.5 μM prostacyclin after washing with the same buffer.

Immunoprecipitation:

Article Title: Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells
Article Snippet: Paragraph title: Immunoprecipitation and immunoblot analysis ... For immunoblot assay, testicular extracts were prepared by homogenization of tissue in a solution containing 50 mM Tris-HCl (pH 7.5), 250 mM sucrose, 1 mM EDTA, a protease inhibitor cocktail and phosphatase inhibitor cocktail [0.4 mM sodium orthovanadate (Wako), 0.4 mM EDTA (Dojindo), 10 mM NaF (Wako) and 10 mM sodium pyrophosphate (Sigma)].

Staining:

Article Title: Transplantation of Olfactory Stem Cells with Biodegradable Hydrogel Accelerates Facial Nerve Regeneration After Crush Injury
Article Snippet: Following perfusion, the head was removed, decalcified in ethylenediaminetetraacetic acid (EDTA, Dojindo Molecular Technologies, Inc.), and embedded in paraffin. .. The heads were then sectioned (3‐μm thick) and stained using the Klüver–Barrera method.

Article Title: Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment
Article Snippet: The platelet preparation was incubated for 30 min at 37 °C with PBS containing 0.1% bovine serum albumin (Roche Diagnostics, Basel, Switzerland); 2 mM EDTA (Dojindo, Kumamoto, Japan); 0.02 unit/ml apyrase (New England BioLabs, Hertfordshire, UK); and 0.5 μM prostacyclin after washing with the same buffer. .. The prepared platelet containing fraction was analysed by flow cytometry using Aria II (Becton Dickinson) after staining with anti-CD41 FITC (eBioscience, San Diego, CA, US) and anti-CD62P APC (eBioscience).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Dojindo Labs ethylenediaminetetraacetic acid edta
    Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl <t>Tris-EDTA</t> (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM <t>ethylenediaminetetraacetic</t> acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.
    Ethylenediaminetetraacetic Acid Edta, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetic acid edta/product/Dojindo Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid edta - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.

    Journal: ACS Omega

    Article Title: Probing Multiple Binding Modes of DNA Hybridization: A Comparison between Single-Molecule Observations and Ensemble Measurements

    doi: 10.1021/acsomega.8b00135

    Figure Lengend Snippet: Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.

    Article Snippet: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased from Dojindo (Kumamoto, Japan).

    Techniques: Binding Assay, Hybridization

    ATP's antimicrobial effect is attributable to its iron-chelating activity. (a) Blocking effects of MgCl 2 and FeCl 3 on ATP's anti- M. intracellulare antimicrobial activity. (b) Anti- M. intracellulare activities of ATP and various metal-chelating agents (pyrophosphate (PPi), EDTA, EGTA) and blocking effects of MgCl 2 . (c) Anti- S. aureus activities of ATP and various metal-chelating agents. (d) Ferric ion-chelating activities of ATP and EDTA measured by CAS assay. (e) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against M. intracellulare . (f) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against P. aeruginosa . (g) Siderophore production by ATP-resistant E. coli during the course of cultivation. (h) Siderophore production by two ATP-resistant K. pneumoniae strains during 48-h cultivation in the presence of ATP. (i) Siderophore production by ATP-susceptible S. aureus and ATP-resistant E. coli during 24-h cultivation in the presence of ATP (5 mM). (j) Siderophore production by ATP-susceptible and ATP-resistant strains of P. aeruginosa during 24-h cultivation.

    Journal: Scientific Reports

    Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions

    doi: 10.1038/srep08610

    Figure Lengend Snippet: ATP's antimicrobial effect is attributable to its iron-chelating activity. (a) Blocking effects of MgCl 2 and FeCl 3 on ATP's anti- M. intracellulare antimicrobial activity. (b) Anti- M. intracellulare activities of ATP and various metal-chelating agents (pyrophosphate (PPi), EDTA, EGTA) and blocking effects of MgCl 2 . (c) Anti- S. aureus activities of ATP and various metal-chelating agents. (d) Ferric ion-chelating activities of ATP and EDTA measured by CAS assay. (e) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against M. intracellulare . (f) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against P. aeruginosa . (g) Siderophore production by ATP-resistant E. coli during the course of cultivation. (h) Siderophore production by two ATP-resistant K. pneumoniae strains during 48-h cultivation in the presence of ATP. (i) Siderophore production by ATP-susceptible S. aureus and ATP-resistant E. coli during 24-h cultivation in the presence of ATP (5 mM). (j) Siderophore production by ATP-susceptible and ATP-resistant strains of P. aeruginosa during 24-h cultivation.

    Article Snippet: Special agents The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), EGTA (Dojindo), dipyridyl (Sigma), CAS (Chrome Azurol S, Sigma), E. coli S17-1 and pK18mobSacB (kindly provided by Dr. F. Taguchi, Okayama University), Instagene matrix (Bio-Rad, Hercules, CA), KOD-Plus (Toyobo, Osaka, Japan), Wizard SV Gel and PCR cleanup system (Promeg, Madison, WI), Ligation High (Toyobo), Protein Assay Rapid Kit (Wako), [14 C] isoleucine (Moravek Biochemicals, Inc, Brea, CA), [14 C]uracil (Moravek Biochemicals, Inc.), α-defensin-1 (Peptide institute, Inc, Osaka, Japan.), cathepsin G (Sigma), vancomycin (Wako), clarithromycin (Taisho-Toyama Pharmaceutical Co., Tokyo), rifampin (Daiichi Sankyo Co., Tokyo), and ethambutol (Sigma), gatifloxacin (Wako), FLUOS (Sigma), LB medium (Invitrogen, San Diego, CA), M9 medium (prepared by our laboratory), Heart infusion agar (Eiken Chemical Co., Tokyo, Japan), Middlebrook 7H9 medium (Becton Dickinson, Cockeysville, MD), and Middlebrook 7H11 medium (Becton Dickinson).

    Techniques: Activity Assay, Blocking Assay

    Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.

    Journal: ACS Omega

    Article Title: Probing Multiple Binding Modes of DNA Hybridization: A Comparison between Single-Molecule Observations and Ensemble Measurements

    doi: 10.1021/acsomega.8b00135

    Figure Lengend Snippet: Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA 12 –dT 12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.

    Article Snippet: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased from Dojindo (Kumamoto, Japan).

    Techniques: Binding Assay, Hybridization

    Effects of chelating agents on TPP + uptake. As control experiments, EDTA (a) or BAPTA (b) was added at a final concentration of 1 mM to untreated cells at the time indicated by the arrow. (c) After E. coli cells were preincubated with 0.5 mM MgCl 2 for 3 min, BAPTA and EDTA were, respectively, added at a final concentration of 1 mM at the times indicated by the first and second arrows. The cells were preincubated with 0.5 mM ethylenediamine (d) or 0.5 mM spermine (e) for 3 min, and EDTA (final concentration, 1 mM) was added at the time indicated by the arrow. EDTA and BAPTA were adjusted to pH 7.2 using Tris.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

    doi: 10.1128/AAC.46.4.1073-1079.2002

    Figure Lengend Snippet: Effects of chelating agents on TPP + uptake. As control experiments, EDTA (a) or BAPTA (b) was added at a final concentration of 1 mM to untreated cells at the time indicated by the arrow. (c) After E. coli cells were preincubated with 0.5 mM MgCl 2 for 3 min, BAPTA and EDTA were, respectively, added at a final concentration of 1 mM at the times indicated by the first and second arrows. The cells were preincubated with 0.5 mM ethylenediamine (d) or 0.5 mM spermine (e) for 3 min, and EDTA (final concentration, 1 mM) was added at the time indicated by the arrow. EDTA and BAPTA were adjusted to pH 7.2 using Tris.

    Article Snippet: The reagents were obtained from the following sources: calcium ionophore II ( N , N , N ′, N ′-tetracyclohexyl-3-oxapentanediamide) and bis(2-ethylhexyl) sebacate were from Fluka (Buchs, Switzerland); potassium tetrakis( p -chlorophenyl)borate (KTpClPB), sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate (NaHFPB), TPP chloride (TPPCl), EDTA, and morpholinepropanesulfonic acid (MOPS) were from Dojindo Laboratories (Kumamoto, Japan); polyvinyl chloride (PVC; degree of polymerization, 1,020) was from Nacalai Tesque (Kyoto, Japan); methoctramine tetrahydrochloride, 1-naphthylacetylspermine trihydrochloride, spermine tetrahydrochloride, spermidine trihydrochloride, benzylamine hydrochloride, 1,2-bis(2-aminophenoxy)ethane- N,N,N ′ N ′-tetraacetic acid (BAPTA), choline chloride, and melittin (from bee venom, approximately 85% by high-performance liquid chromatography) were from Sigma (St. Louis, Mo); N -benzylethylenediamine and 4- N -benzylspermidine were from Aldrich (Milwaukee, Wis.); PMBN was from Boehringer Mannheim (Mannheim, Germany); methylamine hydrochloride and ethylenediamine dihydrochloride were from Tokyo Kasei (Tokyo, Japan); and Tris was from Wako (Osaka, Japan).

    Techniques: Concentration Assay