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Biological Industries Inc ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Biological Industries Inc
Average 99 stars, based on 1 article reviews
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ethylenediaminetetraacetic acid - by Bioz Stars, 2020-03
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Luciferase:

Article Title: Cell-cell contact area affects Notch signaling and Notch-dependent patterning
Article Snippet: .. Cells were lysed with 100 μl/well Passive lysis buffer x1 (promega) for 10 min. 20μl of each sample was used for luciferase activity using filtrated luciferase buffer including: 26 mg of (MgCO3)4 Mg(OH)2 (Sigma), 20 mM Tricine (Sigma), 0.1 mM EDTA (Biological Industries), 2.67 mM pH=7.8 MgSO4 (Merck). .. For the luciferase reaction we used luciferase buffer supplemented with: 0.4 mM ATP (Sigma), 26.6 mM DTT (Sigma), Coenzyme A X0.8 (Sigma) AND 0.4 mM D-Luciferine and for Renilla activity using filtrated Renilla buffer including 80 mM di-Potassium hydrogen phosphate trihydrate (Merck) and 20 mM Potassium dihydrogen phosphate for analysis (Merck).

Synthesized:

Article Title: Study of the cytotoxic effects of 2,5-diaziridinyl-3,6-dimethyl-1,4-benzoquinone (MeDZQ) in mouse hepatoma cells
Article Snippet: It was synthesized according to a previously published procedure (Cameron and Gilles, 1968[ ]; Winski et al., 1998[ ]). .. Fetal bovine serum (FBS) was obtained from Life Technologies (USA), penicillin-streptomycin, trypsin and EDTA were purchased from Biological Industries (Israel).

Cytometry:

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.). .. EPC were characterized using flow cytometry (fluorescence-activated cell sorting, FACS) using fluorescein isothiocyanate-labeled antibodies specific for: CD14, CD34 (mouse anti-human, BD Biosciences, San jose, CA, USA) and CD31 (LifeSpan BioSciences, Seattle, Washington, USA), KDR (mouse anti-human, BD Biosciences).

Article Title: Etk/Bmx Regulates Proteinase-Activated-Receptor1 (PAR1) in Breast Cancer Invasion: Signaling Partners, Hierarchy and Physiological Significance
Article Snippet: Paragraph title: Flow Cytometry Analysis ... The cells were detached from the plates with 0.5 mM EDTA in 0.1 M sodium phosphate at pH 7.4 (Biological Industries), washed and re-suspended in PBS.

Modification:

Article Title: Study of the cytotoxic effects of 2,5-diaziridinyl-3,6-dimethyl-1,4-benzoquinone (MeDZQ) in mouse hepatoma cells
Article Snippet: Fetal bovine serum (FBS) was obtained from Life Technologies (USA), penicillin-streptomycin, trypsin and EDTA were purchased from Biological Industries (Israel). .. Dulbecco's modified Eagle medium (DMEM), acridine orange/ethidium bromide (AO/EB) mixture, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dicumarol (DIC), N,N' -diphenyl-p -phenylenediamine (DPPD), desferrioxamine (DESF), MAPK inhibitors PD098059, SP600125 and SB203580 were obtained from Sigma-Aldrich (USA).

Incubation:

Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
Article Snippet: 5.5 × 105 cells of human pRPE cells (Lonza) were plated in 100 mm culture dishes (Falcon) in RtEGM BulletKit Medium (Lonza) and incubated at 37°C in a humidified 5% CO2 atmosphere. .. The medium was replaced twice weekly, and cells were passaged with 0.25% trypsin/0.1% EDTA (Biological Industries, Israel) upon reaching 90% confluence.

Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
Article Snippet: Subsequently, 1 × 106 cells were plated in 100 mm culture dishes in ADSC medium and incubated at 37°C in a humidified 8% CO2 atmosphere [ ]. .. The medium was changed twice weekly, and cells were passaged with 0.25% trypsin/0.1% EDTA (Biological Industries, Israel) upon reaching 90% confluency.

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.). .. In this study, 5 × 105 cells in PBS were incubated 30 min with antibodies according to the manufacturers’ recommendations.

Article Title: Comparison of Positron Emission Tomography Using 2-[18F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18F]-fluorothymidine in Lung Cancer Imaging
Article Snippet: 2-[18 F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18 F]-fluorothymidine uptake in A549 cells Reagents and cell culture Reagents included high-glucose DMEM (PAA Laboratories GmbH, Hessen, Austria); 1640 culture medium (KeyGEN BioTECH Co., Ltd., Nanjing, China); 10% fetal bovine serum (FBS-O7-003, Bio International Limited, Auckland, New Zealand); 0.1% trypsin + ethylenediaminetetraacetic acid (Biological Industries, Austria); phosphate-buffered saline (PBS; PAA Laboratories GmbH); and 18 F-FDG and 18 F-FLT (provided by the PET Radiopharmaceutical Laboratory of the General Hospital of the People's Liberation Army). .. A549 cells were cultured under routine procedures in RPMI-1640 culture medium and the assay was performed as follows: (1) cells were seeded by 1 × 105 per well into six-well plate, incubated for 24 h, and removed for observation under an inverted telescope to determine cell shape; (2) 18 F-FDG 37.0 MBq was diluted into PBS and 500 µl diluent was added to small test tubes and placed into well-type γ-counting instrument (FJ-367, State-owned No. 267 Company, Beijing, China) to adjust the count to 80,000–100,000; (3) 500 µl adjusted tracers were added into the 6-well plate and placed in a 5% CO2 incubator for cell culture; the same amount of tracers were also added to an empty test tube for the blank control and transferred to the well-type γ-counting instrument for cell counting; (4) at 30, 60, 90, 120, 150, and 180 min, the 6-well plate was removed, the supernatant from each well was taken and washed by PBS three times, 0.5 ml trypsin was added and incubated for 3 min, 1 ml DMEM culture medium was added to neutralize the fluid and mixed, and then the cell suspension liquid was transferred to small test tubes and washed by PBS and transferred to the well-type γ-counting instrument for cell counting; the blank control tubes were also counted; (5) after γ-cell counting, 3 to 6 wells were removed for cell counting and the mean count was used.

Article Title: Etk/Bmx Regulates Proteinase-Activated-Receptor1 (PAR1) in Breast Cancer Invasion: Signaling Partners, Hierarchy and Physiological Significance
Article Snippet: The cells were detached from the plates with 0.5 mM EDTA in 0.1 M sodium phosphate at pH 7.4 (Biological Industries), washed and re-suspended in PBS. .. The cells were analyzed by FACS after incubation for 60 min at 4°C with 10 µg/ml anti- PAR1 -wede-PE antibodies.

Article Title: Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells
Article Snippet: .. When culture reached confluence, cells were treated with 0.05% trypsin (LiStar Fish) for 4 min at 37°C and 0.02% EDTA, replaced in amniodish, and incubated at 37°C for 24 h. For cytogenetic analysis, cultures were incubated for 40 min with Colcimide (100 ng/mL; Beit Haemek), washed with PBS, dissociated with Trypsin (LiStar Fish) for 4 min at 37°C, centrifuged at 100 g for 5 min, resuspended in 1 mL of growth medium into which 5 mL of warm KCl and sodium citrate hypotonic solution (Sigma Chemical Co) were added dropwise, incubated for 30 min at 37°C, followed by fixation at Room Temperature (RT) with 3:1 methanol/acetic acid for 5 min. GTG banding was performed by incubating the glass slides in a 0.05% trypsin solution at 37°C for 15 s, followed by rinsing the slides in phosphate-buffered saline buffer and staining in a 5% Giemsa stain for 8 min. ..

Activity Assay:

Article Title: Cell-cell contact area affects Notch signaling and Notch-dependent patterning
Article Snippet: .. Cells were lysed with 100 μl/well Passive lysis buffer x1 (promega) for 10 min. 20μl of each sample was used for luciferase activity using filtrated luciferase buffer including: 26 mg of (MgCO3)4 Mg(OH)2 (Sigma), 20 mM Tricine (Sigma), 0.1 mM EDTA (Biological Industries), 2.67 mM pH=7.8 MgSO4 (Merck). .. For the luciferase reaction we used luciferase buffer supplemented with: 0.4 mM ATP (Sigma), 26.6 mM DTT (Sigma), Coenzyme A X0.8 (Sigma) AND 0.4 mM D-Luciferine and for Renilla activity using filtrated Renilla buffer including 80 mM di-Potassium hydrogen phosphate trihydrate (Merck) and 20 mM Potassium dihydrogen phosphate for analysis (Merck).

Article Title: Time Frames for Neutralization during the Human Immunodeficiency Virus Type 1 Entry Phase, as Monitored in Synchronously Infected Cell Cultures ▿
Article Snippet: In kinetic measurements of HIV escape from protease-mediated inactivation, cells were treated with digestion buffer containing 2.5 mg/ml trypsin and 0.54 mM EDTA (Biological Industries). .. After 12 min incubation at 37°C, DMEM-10% FCS was added to halt protease activity.

Giemsa Stain:

Article Title: Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells
Article Snippet: .. When culture reached confluence, cells were treated with 0.05% trypsin (LiStar Fish) for 4 min at 37°C and 0.02% EDTA, replaced in amniodish, and incubated at 37°C for 24 h. For cytogenetic analysis, cultures were incubated for 40 min with Colcimide (100 ng/mL; Beit Haemek), washed with PBS, dissociated with Trypsin (LiStar Fish) for 4 min at 37°C, centrifuged at 100 g for 5 min, resuspended in 1 mL of growth medium into which 5 mL of warm KCl and sodium citrate hypotonic solution (Sigma Chemical Co) were added dropwise, incubated for 30 min at 37°C, followed by fixation at Room Temperature (RT) with 3:1 methanol/acetic acid for 5 min. GTG banding was performed by incubating the glass slides in a 0.05% trypsin solution at 37°C for 15 s, followed by rinsing the slides in phosphate-buffered saline buffer and staining in a 5% Giemsa stain for 8 min. ..

Transplantation Assay:

Article Title: Standardization of the Teratoma Assay for Analysis of Pluripotency of Human ES Cells and Biosafety of Their Differentiated Progeny
Article Snippet: .. Preparation of hESCs for Transplantation On the day of transplantation hESCs were harvested and dissociated into a single cell suspension using 0.05% EDTA (Biological Industries, Beit-Haemek, Israel) .To ensure a single cell suspension the cells were filtered through a sterile filter. .. The filtered cells were centrifuged and the pellet was re-suspended in PBS (Invitrogen).

Flow Cytometry:

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.). .. EPC were characterized using flow cytometry (fluorescence-activated cell sorting, FACS) using fluorescein isothiocyanate-labeled antibodies specific for: CD14, CD34 (mouse anti-human, BD Biosciences, San jose, CA, USA) and CD31 (LifeSpan BioSciences, Seattle, Washington, USA), KDR (mouse anti-human, BD Biosciences).

Article Title: Etk/Bmx Regulates Proteinase-Activated-Receptor1 (PAR1) in Breast Cancer Invasion: Signaling Partners, Hierarchy and Physiological Significance
Article Snippet: Paragraph title: Flow Cytometry Analysis ... The cells were detached from the plates with 0.5 mM EDTA in 0.1 M sodium phosphate at pH 7.4 (Biological Industries), washed and re-suspended in PBS.

Cell Culture:

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: The attached cells were continuously cultured with complete EGM-2 medium. .. Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.).

Article Title: Comparison of Positron Emission Tomography Using 2-[18F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18F]-fluorothymidine in Lung Cancer Imaging
Article Snippet: .. 2-[18 F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18 F]-fluorothymidine uptake in A549 cells Reagents and cell culture Reagents included high-glucose DMEM (PAA Laboratories GmbH, Hessen, Austria); 1640 culture medium (KeyGEN BioTECH Co., Ltd., Nanjing, China); 10% fetal bovine serum (FBS-O7-003, Bio International Limited, Auckland, New Zealand); 0.1% trypsin + ethylenediaminetetraacetic acid (Biological Industries, Austria); phosphate-buffered saline (PBS; PAA Laboratories GmbH); and 18 F-FDG and 18 F-FLT (provided by the PET Radiopharmaceutical Laboratory of the General Hospital of the People's Liberation Army). .. Cell culture and probe binding assay Lung cancer A549 cells were provided by the Institute of General Surgery of General Hospital of PLA.

Article Title: Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells
Article Snippet: Metaphase chromosomes were prepared from hPDLSCs cultured with MSCGM-CD. .. When culture reached confluence, cells were treated with 0.05% trypsin (LiStar Fish) for 4 min at 37°C and 0.02% EDTA, replaced in amniodish, and incubated at 37°C for 24 h. For cytogenetic analysis, cultures were incubated for 40 min with Colcimide (100 ng/mL; Beit Haemek), washed with PBS, dissociated with Trypsin (LiStar Fish) for 4 min at 37°C, centrifuged at 100 g for 5 min, resuspended in 1 mL of growth medium into which 5 mL of warm KCl and sodium citrate hypotonic solution (Sigma Chemical Co) were added dropwise, incubated for 30 min at 37°C, followed by fixation at Room Temperature (RT) with 3:1 methanol/acetic acid for 5 min. GTG banding was performed by incubating the glass slides in a 0.05% trypsin solution at 37°C for 15 s, followed by rinsing the slides in phosphate-buffered saline buffer and staining in a 5% Giemsa stain for 8 min.

other:

Article Title: Aspergillus fumigatus enhances human NK cell activity by regulating M1 macrophage polarization
Article Snippet: Macrophage stimulation experiment Macrophages were collected following digestion by 0.2% EDTA at 37°C for 5 min (cat. no. 02-032-1ACS; Biological Industries).

Binding Assay:

Article Title: Comparison of Positron Emission Tomography Using 2-[18F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18F]-fluorothymidine in Lung Cancer Imaging
Article Snippet: 2-[18 F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18 F]-fluorothymidine uptake in A549 cells Reagents and cell culture Reagents included high-glucose DMEM (PAA Laboratories GmbH, Hessen, Austria); 1640 culture medium (KeyGEN BioTECH Co., Ltd., Nanjing, China); 10% fetal bovine serum (FBS-O7-003, Bio International Limited, Auckland, New Zealand); 0.1% trypsin + ethylenediaminetetraacetic acid (Biological Industries, Austria); phosphate-buffered saline (PBS; PAA Laboratories GmbH); and 18 F-FDG and 18 F-FLT (provided by the PET Radiopharmaceutical Laboratory of the General Hospital of the People's Liberation Army). .. Cell culture and probe binding assay Lung cancer A549 cells were provided by the Institute of General Surgery of General Hospital of PLA.

Reporter Gene Assay:

Article Title: Cell-cell contact area affects Notch signaling and Notch-dependent patterning
Article Snippet: Notch Activity assay The activity of N1G4-citrine was tested using luciferase reporter gene assay. .. Cells were lysed with 100 μl/well Passive lysis buffer x1 (promega) for 10 min. 20μl of each sample was used for luciferase activity using filtrated luciferase buffer including: 26 mg of (MgCO3)4 Mg(OH)2 (Sigma), 20 mM Tricine (Sigma), 0.1 mM EDTA (Biological Industries), 2.67 mM pH=7.8 MgSO4 (Merck).

MTT Assay:

Article Title: Study of the cytotoxic effects of 2,5-diaziridinyl-3,6-dimethyl-1,4-benzoquinone (MeDZQ) in mouse hepatoma cells
Article Snippet: Fetal bovine serum (FBS) was obtained from Life Technologies (USA), penicillin-streptomycin, trypsin and EDTA were purchased from Biological Industries (Israel). .. Dulbecco's modified Eagle medium (DMEM), acridine orange/ethidium bromide (AO/EB) mixture, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dicumarol (DIC), N,N' -diphenyl-p -phenylenediamine (DPPD), desferrioxamine (DESF), MAPK inhibitors PD098059, SP600125 and SB203580 were obtained from Sigma-Aldrich (USA).

Fluorescence:

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.). .. EPC were characterized using flow cytometry (fluorescence-activated cell sorting, FACS) using fluorescein isothiocyanate-labeled antibodies specific for: CD14, CD34 (mouse anti-human, BD Biosciences, San jose, CA, USA) and CD31 (LifeSpan BioSciences, Seattle, Washington, USA), KDR (mouse anti-human, BD Biosciences).

Isolation:

Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
Article Snippet: Paragraph title: 2.1.1. Isolation and Culture of Human Adipose Tissue-Derived Stem Cells (ASCs) ... The medium was changed twice weekly, and cells were passaged with 0.25% trypsin/0.1% EDTA (Biological Industries, Israel) upon reaching 90% confluency.

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: Paragraph title: Isolation, expansion and characterization of early and late EPCs ... Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.).

Transfection:

Article Title: Cell-cell contact area affects Notch signaling and Notch-dependent patterning
Article Snippet: 24 hours after transfection, the cells were trypsinized and transferred to dishes with or without plate-adsorbed Delta-like 1 fused to immunoglobin-G (Dll1-Fc, a kind gift from Irwin Bernstein). .. Cells were lysed with 100 μl/well Passive lysis buffer x1 (promega) for 10 min. 20μl of each sample was used for luciferase activity using filtrated luciferase buffer including: 26 mg of (MgCO3)4 Mg(OH)2 (Sigma), 20 mM Tricine (Sigma), 0.1 mM EDTA (Biological Industries), 2.67 mM pH=7.8 MgSO4 (Merck).

Cell Adhesion Assay:

Article Title: Bioaccessibility of Shore Magic® collagen, a low-molecular-weight collagen supplement, in different in vitro barrier models
Article Snippet: Paragraph title: 2.6. Adhesion assay ... The next day, a trypsin/EDTA solution B containing 0.25% trypsin and 0.05% EDTA in Puck's Saline A (Biological Industries) was used to detach the cells from the plate.

Staining:

Article Title: Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells
Article Snippet: .. When culture reached confluence, cells were treated with 0.05% trypsin (LiStar Fish) for 4 min at 37°C and 0.02% EDTA, replaced in amniodish, and incubated at 37°C for 24 h. For cytogenetic analysis, cultures were incubated for 40 min with Colcimide (100 ng/mL; Beit Haemek), washed with PBS, dissociated with Trypsin (LiStar Fish) for 4 min at 37°C, centrifuged at 100 g for 5 min, resuspended in 1 mL of growth medium into which 5 mL of warm KCl and sodium citrate hypotonic solution (Sigma Chemical Co) were added dropwise, incubated for 30 min at 37°C, followed by fixation at Room Temperature (RT) with 3:1 methanol/acetic acid for 5 min. GTG banding was performed by incubating the glass slides in a 0.05% trypsin solution at 37°C for 15 s, followed by rinsing the slides in phosphate-buffered saline buffer and staining in a 5% Giemsa stain for 8 min. ..

Positron Emission Tomography:

Article Title: Comparison of Positron Emission Tomography Using 2-[18F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18F]-fluorothymidine in Lung Cancer Imaging
Article Snippet: .. 2-[18 F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18 F]-fluorothymidine uptake in A549 cells Reagents and cell culture Reagents included high-glucose DMEM (PAA Laboratories GmbH, Hessen, Austria); 1640 culture medium (KeyGEN BioTECH Co., Ltd., Nanjing, China); 10% fetal bovine serum (FBS-O7-003, Bio International Limited, Auckland, New Zealand); 0.1% trypsin + ethylenediaminetetraacetic acid (Biological Industries, Austria); phosphate-buffered saline (PBS; PAA Laboratories GmbH); and 18 F-FDG and 18 F-FLT (provided by the PET Radiopharmaceutical Laboratory of the General Hospital of the People's Liberation Army). .. Cell culture and probe binding assay Lung cancer A549 cells were provided by the Institute of General Surgery of General Hospital of PLA.

Wound Healing Assay:

Article Title: Bioaccessibility of Shore Magic® collagen, a low-molecular-weight collagen supplement, in different in vitro barrier models
Article Snippet: As the wound healing assay, SMC 1, 3 and 5 mg/ml in serum-free medium was pre-incubated in a 24-well plate. .. The next day, a trypsin/EDTA solution B containing 0.25% trypsin and 0.05% EDTA in Puck's Saline A (Biological Industries) was used to detach the cells from the plate.

Proximity Ligation Assay:

Article Title: Comparison of Positron Emission Tomography Using 2-[18F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18F]-fluorothymidine in Lung Cancer Imaging
Article Snippet: 2-[18 F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18 F]-fluorothymidine uptake in A549 cells Reagents and cell culture Reagents included high-glucose DMEM (PAA Laboratories GmbH, Hessen, Austria); 1640 culture medium (KeyGEN BioTECH Co., Ltd., Nanjing, China); 10% fetal bovine serum (FBS-O7-003, Bio International Limited, Auckland, New Zealand); 0.1% trypsin + ethylenediaminetetraacetic acid (Biological Industries, Austria); phosphate-buffered saline (PBS; PAA Laboratories GmbH); and 18 F-FDG and 18 F-FLT (provided by the PET Radiopharmaceutical Laboratory of the General Hospital of the People's Liberation Army). .. Cell culture and probe binding assay Lung cancer A549 cells were provided by the Institute of General Surgery of General Hospital of PLA.

Cell Counting:

Article Title: Comparison of Positron Emission Tomography Using 2-[18F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18F]-fluorothymidine in Lung Cancer Imaging
Article Snippet: 2-[18 F]-fluoro-2-deoxy-D-glucose and 3-deoxy-3-[18 F]-fluorothymidine uptake in A549 cells Reagents and cell culture Reagents included high-glucose DMEM (PAA Laboratories GmbH, Hessen, Austria); 1640 culture medium (KeyGEN BioTECH Co., Ltd., Nanjing, China); 10% fetal bovine serum (FBS-O7-003, Bio International Limited, Auckland, New Zealand); 0.1% trypsin + ethylenediaminetetraacetic acid (Biological Industries, Austria); phosphate-buffered saline (PBS; PAA Laboratories GmbH); and 18 F-FDG and 18 F-FLT (provided by the PET Radiopharmaceutical Laboratory of the General Hospital of the People's Liberation Army). .. A549 cells were cultured under routine procedures in RPMI-1640 culture medium and the assay was performed as follows: (1) cells were seeded by 1 × 105 per well into six-well plate, incubated for 24 h, and removed for observation under an inverted telescope to determine cell shape; (2) 18 F-FDG 37.0 MBq was diluted into PBS and 500 µl diluent was added to small test tubes and placed into well-type γ-counting instrument (FJ-367, State-owned No. 267 Company, Beijing, China) to adjust the count to 80,000–100,000; (3) 500 µl adjusted tracers were added into the 6-well plate and placed in a 5% CO2 incubator for cell culture; the same amount of tracers were also added to an empty test tube for the blank control and transferred to the well-type γ-counting instrument for cell counting; (4) at 30, 60, 90, 120, 150, and 180 min, the 6-well plate was removed, the supernatant from each well was taken and washed by PBS three times, 0.5 ml trypsin was added and incubated for 3 min, 1 ml DMEM culture medium was added to neutralize the fluid and mixed, and then the cell suspension liquid was transferred to small test tubes and washed by PBS and transferred to the well-type γ-counting instrument for cell counting; the blank control tubes were also counted; (5) after γ-cell counting, 3 to 6 wells were removed for cell counting and the mean count was used.

Lysis:

Article Title: Cell-cell contact area affects Notch signaling and Notch-dependent patterning
Article Snippet: .. Cells were lysed with 100 μl/well Passive lysis buffer x1 (promega) for 10 min. 20μl of each sample was used for luciferase activity using filtrated luciferase buffer including: 26 mg of (MgCO3)4 Mg(OH)2 (Sigma), 20 mM Tricine (Sigma), 0.1 mM EDTA (Biological Industries), 2.67 mM pH=7.8 MgSO4 (Merck). .. For the luciferase reaction we used luciferase buffer supplemented with: 0.4 mM ATP (Sigma), 26.6 mM DTT (Sigma), Coenzyme A X0.8 (Sigma) AND 0.4 mM D-Luciferine and for Renilla activity using filtrated Renilla buffer including 80 mM di-Potassium hydrogen phosphate trihydrate (Merck) and 20 mM Potassium dihydrogen phosphate for analysis (Merck).

FACS:

Article Title: Endothelial Progenitor Cells inhibit jaw osteonecrosis in a rat model: A major adverse effect of bisphosphonate therapy
Article Snippet: Cells were fed three times per week and were split when they reached ~80% confluent by brief trypsinization using 0.5% trypsine in 0.2% ethylenediaminetetraacetic acid (EDTA; BI, Beit-Haemek, Israel.). .. EPC were characterized using flow cytometry (fluorescence-activated cell sorting, FACS) using fluorescein isothiocyanate-labeled antibodies specific for: CD14, CD34 (mouse anti-human, BD Biosciences, San jose, CA, USA) and CD31 (LifeSpan BioSciences, Seattle, Washington, USA), KDR (mouse anti-human, BD Biosciences).

Article Title: Etk/Bmx Regulates Proteinase-Activated-Receptor1 (PAR1) in Breast Cancer Invasion: Signaling Partners, Hierarchy and Physiological Significance
Article Snippet: The cells were detached from the plates with 0.5 mM EDTA in 0.1 M sodium phosphate at pH 7.4 (Biological Industries), washed and re-suspended in PBS. .. The cells were analyzed by FACS after incubation for 60 min at 4°C with 10 µg/ml anti- PAR1 -wede-PE antibodies.

Fluorescence In Situ Hybridization:

Article Title: Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells
Article Snippet: .. When culture reached confluence, cells were treated with 0.05% trypsin (LiStar Fish) for 4 min at 37°C and 0.02% EDTA, replaced in amniodish, and incubated at 37°C for 24 h. For cytogenetic analysis, cultures were incubated for 40 min with Colcimide (100 ng/mL; Beit Haemek), washed with PBS, dissociated with Trypsin (LiStar Fish) for 4 min at 37°C, centrifuged at 100 g for 5 min, resuspended in 1 mL of growth medium into which 5 mL of warm KCl and sodium citrate hypotonic solution (Sigma Chemical Co) were added dropwise, incubated for 30 min at 37°C, followed by fixation at Room Temperature (RT) with 3:1 methanol/acetic acid for 5 min. GTG banding was performed by incubating the glass slides in a 0.05% trypsin solution at 37°C for 15 s, followed by rinsing the slides in phosphate-buffered saline buffer and staining in a 5% Giemsa stain for 8 min. ..

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    Biological Industries Inc trypsinization
    Dependence of R min on surface coverage f (estimated from v min ) during cell deposition ( solid circles ) and detachment after <t>trypsinization</t> ( open circles ). Although the rates of these two processes are very different, the R min ( f ) dependences are almost
    Trypsinization, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biological Industries Inc trypsin edta
    Dependence of R min on surface coverage f (estimated from v min ) during cell deposition ( solid circles ) and detachment after <t>trypsinization</t> ( open circles ). Although the rates of these two processes are very different, the R min ( f ) dependences are almost
    Trypsin Edta, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta/product/Biological Industries Inc
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    Biological Industries Inc edta
    Dependence of R min on surface coverage f (estimated from v min ) during cell deposition ( solid circles ) and detachment after <t>trypsinization</t> ( open circles ). Although the rates of these two processes are very different, the R min ( f ) dependences are almost
    Edta, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 3 article reviews
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    Dependence of R min on surface coverage f (estimated from v min ) during cell deposition ( solid circles ) and detachment after trypsinization ( open circles ). Although the rates of these two processes are very different, the R min ( f ) dependences are almost

    Journal: Biophysical Journal

    Article Title: Real-Time Monitoring of Epithelial Cell-Cell and Cell-Substrate Interactions by Infrared Surface Plasmon Spectroscopy

    doi: 10.1016/j.bpj.2010.10.017

    Figure Lengend Snippet: Dependence of R min on surface coverage f (estimated from v min ) during cell deposition ( solid circles ) and detachment after trypsinization ( open circles ). Although the rates of these two processes are very different, the R min ( f ) dependences are almost

    Article Snippet: Cells were prepared for the SPR experiments as follows: MDCK cells of a confluent monolayer were detached from the dish by trypsinization (0.25% trypsin/EDTA in Puck's saline A; Biological Industries, Israel).

    Techniques: