ethylenediaminetetra­acetic acid  (Millipore)


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    Name:
    Ethylenediaminetetraacetic acid
    Description:
    Ethylenediaminetetraacetic acid EDTA is a hydrophilic metal chelating agent that transforms metal ions into inactive cyclic metal complexes Hence it has industrial application for resolving metal contamination
    Catalog Number:
    e9884
    Price:
    None
    Applications:
    Ethylenediaminetetraacetic acid has been used: . as a component of labeling buffer, which is used in the washing step during cell fractionation{155) . for investigating the effect of papain enzyme on wound debridement and other skin issues . for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis
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    Structured Review

    Millipore ethylenediaminetetra­acetic acid
    Ethylenediaminetetraacetic acid
    Ethylenediaminetetraacetic acid EDTA is a hydrophilic metal chelating agent that transforms metal ions into inactive cyclic metal complexes Hence it has industrial application for resolving metal contamination
    https://www.bioz.com/result/ethylenediaminetetra­acetic acid/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetra­acetic acid - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Monitoring bone changes due to calcium, magnesium, and phosphorus loss in rat femurs using Quantitative Ultrasound
    Article Snippet: .. The demineralization protocol consisted of immersing the rat femur samples in a 25-mL solution of Ethylenediaminetetraacetic Acid (EDTA) disodium salt (Sigma-Aldrich®, Missouri, USA) at pH = 8 and 0.376 M concentration for 24 h at 25.0 ± 1.5 °C, and a new solution and new reservoir were used each day . ..

    Protein Extraction:

    Article Title: SUMOylation of HNF4? regulates protein stability and hepatocyte function
    Article Snippet: .. Cellular protein extraction Cells grown in a six-well plate were lysed in 150 µl of SUMO lysis buffer [2% SDS, 50 mM Tris-HCl, pH 8, 1 mM ethylenediaminetetraacetic acid (EDTA) and 10 mM iodoacetamide (all from Sigma-Aldrich)] for 5 minutes at room temperature. ..

    Sulforhodamine B Assay:

    Article Title: Enhanced uptake and transport of (+)-catechin and (-)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells
    Article Snippet: .. Chemicals and reagents Catechin, EGCG, sorbitan monostearate (Span 60), CH, dihexadecyl phosphate, fluorescein isothiocyanate (FITC), sulforhodamine B (SRB), fluorescein sodium salt, ascorbic acid (AA), sodium azide, verapamil, 5-(3-(2-(7-chloroquinolin-2-yl)ethenyl)phenyl)-8-dimethylcarbamyl-4,6-dithiaoctanoic acid sodium salt hydrate (MK-571), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich (St Louis, MO, USA). ..

    Lysis:

    Article Title: SUMOylation of HNF4? regulates protein stability and hepatocyte function
    Article Snippet: .. Cellular protein extraction Cells grown in a six-well plate were lysed in 150 µl of SUMO lysis buffer [2% SDS, 50 mM Tris-HCl, pH 8, 1 mM ethylenediaminetetraacetic acid (EDTA) and 10 mM iodoacetamide (all from Sigma-Aldrich)] for 5 minutes at room temperature. ..

    Article Title: Internalization Mechanisms of the Epidermal Growth Factor Receptor after Activation with Different Ligands
    Article Snippet: .. Cells were rinsed in cold PBS and scraped off in RIPA lysis buffer (1% NP40, 20 mM MOPS, 0.1% SDS, 1% Na-deoxycholate, 150 mM NaCl, and 1 mM ethylenediaminetetraacetic acid) supplemented with Protease Inhibitor Cocktail Set II and Phosphatase Inhibitor Cocktail Set III (Calbiochem). .. Cell debris was removed by centrifugation.

    Protease Inhibitor:

    Article Title: Internalization Mechanisms of the Epidermal Growth Factor Receptor after Activation with Different Ligands
    Article Snippet: .. Cells were rinsed in cold PBS and scraped off in RIPA lysis buffer (1% NP40, 20 mM MOPS, 0.1% SDS, 1% Na-deoxycholate, 150 mM NaCl, and 1 mM ethylenediaminetetraacetic acid) supplemented with Protease Inhibitor Cocktail Set II and Phosphatase Inhibitor Cocktail Set III (Calbiochem). .. Cell debris was removed by centrifugation.

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  • 99
    Millipore ethylenediaminetetraacetic acid
    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with <t>ethylenediaminetetraacetic</t> acid shows absence of activity. (C)
    Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Millipore
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Journal: Tissue Engineering. Part A

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue

    doi: 10.1089/ten.tea.2018.0036

    Figure Lengend Snippet: Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Article Snippet: Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control.

    Techniques: Expressing, Activity Assay, RNA Sequencing Assay, Zymography, Recombinant, Inhibition