ethylene glycol tetraacetic acid (Millipore)
97
Structured Review
Millipore
ethylene glycol tetraacetic acid
Ethylene Glycol Tetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylene glycol tetraacetic acid/product/Millipore
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ethylene Glycol Tetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylene glycol tetraacetic acid/product/Millipore
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethylene glycol tetraacetic acid - by Bioz Stars,
2021-03
97/100 stars
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![Ca 2+ influx via TRPM2 was required for HG-induced NLRP3 inflammasome activation in U937 cells. ( A ) Fluorescence images showing fluo-4-loaded cells taken at indicated timepoints, and representative flow cytometric plot and graph showing relative median fluorescence intensity (MFI) of intracellular Ca 2+ concentration by Fluo-4 staining under low glucose (LG; 5.5 mM glucose;) or high glucose (HG; 30 mM glucose) (n = 5). ( B ) Relative changes in [Ca 2+ ] i , evoked by H 2 O 2 (1 mM) over the time course. The cells were treated in the presence of <t>2-aminoethoxydiphenyl</t> borate (2-APB; 100 μM), or with pre-treatment of 3-aminobenzamide (3-AB; 5 mM) or 3,4-dihydro-5-[4-(1-piperidinyl)butoxy]-1(2H)-isoquinolinone (DPQ; 100 μM), or GAPDH- (GA si) or TRPM2-siRNAs (TRPM2 si) under HG (n = 4). ( C ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), and GAPDH in the presence of <t>EGTA-AM</t> (0.5, 1, 5 mM) under HG (n = 4). ( D ) Immunofluorescence images showing the location of caspase-1 and ASC in fixed cells using confocal microscopy in the presence of GAPDH- or TRPM2-siRNAs, or EGTA-AM (5 mM) under HG. The percentage of co-localization of caspase-1 with ASC was calculated as the average volume of the overlapping areas (n = 4). Data were shown as mean ± S.E.M. ( A ) *P](https://storage.googleapis.com/bioz_article_images/PMC5059733/srep35016-f3.jpg)
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