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Nacalai ethylene glycol tetraacetic acid egta
Ethylene Glycol Tetraacetic Acid Egta, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethylene glycol tetraacetic acid egta - by Bioz Stars, 2020-07
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Lysis:

Article Title: Disease modifying effect of adiponectin in model of α-synucleinopathies
Article Snippet: .. In some experiments, cells were dissolved in lysis buffer: 1% Nonidet P-40, 50 mmol/L HEPES, 150 mmol/L NaCl, 10% glycerol, 1.5 mmol/L MgCl2 , 1 mmol/L ethylene glycol tetraacetic acid (EGTA), and 100 mmol/L sodium fluoride-containing protease inhibitor mixture (Nacalai Tesque, Tokyo, Japan). ..

Protein Extraction:

Article Title: Dexamethasone and BCAA Failed to Modulate Muscle Mass and mTOR Signaling in GH-Deficient Rats
Article Snippet: .. Protein extraction and western blotting Muscle homogenates were prepared in a 1:10 ratio of ice-cold homogenizing buffer (20 mM Tris pH 8.0, 1% Nonidet-P-40, 120 mM NaCl, 20 mM NaF, 1 mM ethylene diamine tetraacetic acid (EDTA), 1 mM ethylene glycol tetraacetic acid (EGTA), 30 mM β-glycerophosphate, 15 mM sodium dihydrogen pyrophosphate, 2 mM sodium orthovanadate and Protease Inhibitory Cocktail (Nacalai Tesque, Kyoto, Japan)) using a polytron homogenizer. ..

Protease Inhibitor:

Article Title: Disease modifying effect of adiponectin in model of α-synucleinopathies
Article Snippet: .. In some experiments, cells were dissolved in lysis buffer: 1% Nonidet P-40, 50 mmol/L HEPES, 150 mmol/L NaCl, 10% glycerol, 1.5 mmol/L MgCl2 , 1 mmol/L ethylene glycol tetraacetic acid (EGTA), and 100 mmol/L sodium fluoride-containing protease inhibitor mixture (Nacalai Tesque, Tokyo, Japan). ..

Western Blot:

Article Title: Dexamethasone and BCAA Failed to Modulate Muscle Mass and mTOR Signaling in GH-Deficient Rats
Article Snippet: .. Protein extraction and western blotting Muscle homogenates were prepared in a 1:10 ratio of ice-cold homogenizing buffer (20 mM Tris pH 8.0, 1% Nonidet-P-40, 120 mM NaCl, 20 mM NaF, 1 mM ethylene diamine tetraacetic acid (EDTA), 1 mM ethylene glycol tetraacetic acid (EGTA), 30 mM β-glycerophosphate, 15 mM sodium dihydrogen pyrophosphate, 2 mM sodium orthovanadate and Protease Inhibitory Cocktail (Nacalai Tesque, Kyoto, Japan)) using a polytron homogenizer. ..

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    Nacalai n tetra acetic acid egta
    Cathepsin G-induced E-cadherin/catenin complex formation and E-cadherin-mediated cell-cell adhesion of MCF-7 cells. MCF-7 cells were cultured in dishes coated with fibronectin for 24 hours. After washing, adherent cells were incubated in serum-free medium without or with 0.5 mU/mL cathepsin G. At each indicated culture time, the cells were lysed, and E-cadherin/catenin complex formation of MCF-7 cells was analyzed by immunoprecipitation and western blot analysis as described in Materials and Methods. (a) and (b) Immunocomplexes with anti-E-cadherin were analyzed by immunoblotting using an anti- <t>β</t> -catenin (a) or anti- α -catenin antibody (b). Whole-cell lysates (WCLs) were immunoblotted with an anti- β -catenin (a) or anti- α -catenin antibody (b). (c) BALB-MC.E12 mouse mammary tumor cells were analyzed as shown in (a). (d) Treatments inhibiting E-cadherin-mediated cell-cell adhesion disrupt cathepsin G-induced cell condensation. MCF-7 cells were cultured in 5% FBS-containing medium on fibronectin for 24 hours. After washing, cell condensation was induced by cathepsin G for 24 hours. Condensed cells were then subjected to serum-free medium supplemented with 400 μ M <t>EGTA</t> for 6 hours or HECD-1 (100 μ g/mL) for 24 hours and then analyzed by phase-contrast microscopy. Cathepsin G-induced cell condensation was analyzed at the original magnification: ×200.
    N Tetra Acetic Acid Egta, supplied by Nacalai, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n tetra acetic acid egta/product/Nacalai
    Average 88 stars, based on 1 article reviews
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    n tetra acetic acid egta - by Bioz Stars, 2020-07
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    93
    Nacalai egta
    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and <t>EGTA</t> or <t>EDTA</t> was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the
    Egta, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Nacalai
    Average 93 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2020-07
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    90
    Nacalai pem buffer
    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and <t>EGTA</t> or <t>EDTA</t> was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the
    Pem Buffer, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pem buffer/product/Nacalai
    Average 90 stars, based on 1 article reviews
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    pem buffer - by Bioz Stars, 2020-07
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    Cathepsin G-induced E-cadherin/catenin complex formation and E-cadherin-mediated cell-cell adhesion of MCF-7 cells. MCF-7 cells were cultured in dishes coated with fibronectin for 24 hours. After washing, adherent cells were incubated in serum-free medium without or with 0.5 mU/mL cathepsin G. At each indicated culture time, the cells were lysed, and E-cadherin/catenin complex formation of MCF-7 cells was analyzed by immunoprecipitation and western blot analysis as described in Materials and Methods. (a) and (b) Immunocomplexes with anti-E-cadherin were analyzed by immunoblotting using an anti- β -catenin (a) or anti- α -catenin antibody (b). Whole-cell lysates (WCLs) were immunoblotted with an anti- β -catenin (a) or anti- α -catenin antibody (b). (c) BALB-MC.E12 mouse mammary tumor cells were analyzed as shown in (a). (d) Treatments inhibiting E-cadherin-mediated cell-cell adhesion disrupt cathepsin G-induced cell condensation. MCF-7 cells were cultured in 5% FBS-containing medium on fibronectin for 24 hours. After washing, cell condensation was induced by cathepsin G for 24 hours. Condensed cells were then subjected to serum-free medium supplemented with 400 μ M EGTA for 6 hours or HECD-1 (100 μ g/mL) for 24 hours and then analyzed by phase-contrast microscopy. Cathepsin G-induced cell condensation was analyzed at the original magnification: ×200.

    Journal: Mediators of Inflammation

    Article Title: Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells

    doi: 10.1155/2009/850940

    Figure Lengend Snippet: Cathepsin G-induced E-cadherin/catenin complex formation and E-cadherin-mediated cell-cell adhesion of MCF-7 cells. MCF-7 cells were cultured in dishes coated with fibronectin for 24 hours. After washing, adherent cells were incubated in serum-free medium without or with 0.5 mU/mL cathepsin G. At each indicated culture time, the cells were lysed, and E-cadherin/catenin complex formation of MCF-7 cells was analyzed by immunoprecipitation and western blot analysis as described in Materials and Methods. (a) and (b) Immunocomplexes with anti-E-cadherin were analyzed by immunoblotting using an anti- β -catenin (a) or anti- α -catenin antibody (b). Whole-cell lysates (WCLs) were immunoblotted with an anti- β -catenin (a) or anti- α -catenin antibody (b). (c) BALB-MC.E12 mouse mammary tumor cells were analyzed as shown in (a). (d) Treatments inhibiting E-cadherin-mediated cell-cell adhesion disrupt cathepsin G-induced cell condensation. MCF-7 cells were cultured in 5% FBS-containing medium on fibronectin for 24 hours. After washing, cell condensation was induced by cathepsin G for 24 hours. Condensed cells were then subjected to serum-free medium supplemented with 400 μ M EGTA for 6 hours or HECD-1 (100 μ g/mL) for 24 hours and then analyzed by phase-contrast microscopy. Cathepsin G-induced cell condensation was analyzed at the original magnification: ×200.

    Article Snippet: These condensed cells were then incubated in serum-free medium supplemented with 400 μ M ethylene glycol-bis-(β -amino-ethyl ether) N , N , N ′, N ′-tetra-acetic acid (EGTA) (Nacalai Tesque, Kyoto, Japan) for 6 hours or 100 μ g/mL anti-E-cadherin antibody (HECD-1; Calbiochem) for 24 hours.

    Techniques: Cell Culture, Incubation, Immunoprecipitation, Western Blot, Microscopy

    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and EGTA or EDTA was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the

    Journal: Infection and Immunity

    Article Title: Listeriolysin O-Dependent Bacterial Entry into the Cytoplasm Is Required for Calpain Activation and Interleukin-1? Secretion in Macrophages Infected with Listeria monocytogenes ▿

    doi: 10.1128/IAI.01143-09

    Figure Lengend Snippet: Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and EGTA or EDTA was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the

    Article Snippet: EDTA and EGTA were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Infection