ethylene glycol tetraacetic acid egta (Millipore)
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Ethylene Glycol Tetraacetic Acid Egta, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylene glycol tetraacetic acid egta/product/Millipore
Average 97 stars, based on 74 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Inflammatory Effects of Menthol vs. Non-menthol Cigarette Smoke Extract on Human Lung Epithelial Cells: A Double-Hit on TRPM8 by Reactive Oxygen Species and Menthol"
Article Title: Inflammatory Effects of Menthol vs. Non-menthol Cigarette Smoke Extract on Human Lung Epithelial Cells: A Double-Hit on TRPM8 by Reactive Oxygen Species and Menthol
Journal: Frontiers in Physiology
doi: 10.3389/fphys.2017.00263

Figure Legend Snippet: Roles of ROS and TRPM8 in the increased intracellular Ca 2+ level induced by M-CSE and Non-M-CSE in HBECs . Intracellular Ca 2+ levels were measured by Fluo-8 fluorescent probe assay. (A) Cells were exposed to medium alone (control), 1% Non-M-CSE, or 1% M-CSE for 1, 2, 5, 10, and 30 min. (B) Representative images of fluorescence-positive cells at 5 min after exposure. (C) Cells were pretreated with N-acetyl-cysteine (NAC), AMTB, or ethylene glycol tetraacetic acid (EGTA) for 1 h and then exposed to medium alone, 1% Non-M-CSE, or 1% M-CSE for 5 min. (D) Cells were transfected with control plasmid or TRPM8 Double Nickase plasmid (KO) and then exposed to medium alone, 1% Non-M-CSE, or 1% M-CSE for 5 min. Data from each group are means ± SEM from four independent experiments. * p
Techniques Used: Fluorescence, Transfection, Plasmid Preparation
2) Product Images from "TRPM8 Channel Activation Induced by Monoterpenoid Rotundifolone Underlies Mesenteric Artery Relaxation"
Article Title: TRPM8 Channel Activation Induced by Monoterpenoid Rotundifolone Underlies Mesenteric Artery Relaxation
Journal: PLoS ONE
doi: 10.1371/journal.pone.0143171
![... -free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased ... Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p](https://storage.googleapis.com/bioz_article_images/PMC4657920/pone.0143171.g008.jpg)
Figure Legend Snippet: Rotundifolone-induced Ca 2+ transient in myocytes depend of extracellular Ca 2+ . (A) Representative recording of the Ca 2+ signals induced by rotundifolone (10 −3 and 3x10 -3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca 2+ signal induced after perfusion with different concentrations of rotundifolone (3x10 -4 , n = 9; 10 −3 , n = 5; 3x10 -3 M, n = 11). (C) Representative recording of Ca 2+ oscillations induced by rotundifolone (3x10 -3 M) in myocytes placed in Ca 2+ -free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased amplitude of the Ca 2+ signal induced by rotundifolone in Ca 2+ -free medium (n = 13). The Ca 2+ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p
Techniques Used: Fluorescence
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