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Amresco ethylene glycol tetraacetic acid egta
Ethylene Glycol Tetraacetic Acid Egta, supplied by Amresco, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylene glycol tetraacetic acid egta/product/Amresco
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
ethylene glycol tetraacetic acid egta - by Bioz Stars, 2020-07
93/100 stars

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MTT Assay:

Article Title: The Involvement of PI3K-Mediated and L-VGCC-Gated Transient Ca2+ Influx in 17?-Estradiol-Mediated Protection of Retinal Cells from H2O2-Induced Apoptosis with Ca2+ Overload
Article Snippet: .. Trypsin, DMSO, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and ethylene glycol tetraacetic acid (EGTA), an extracellular Ca2+ chelator, were purchased from Amresco (Solon, Ohio, USA). .. LY294002, a PI3K inhibitor, was purchased from Cayman (Ann Arbor, MI, USA).

other:

Article Title: Mitochondrial flashes regulate ATP homeostasis in the heart
Article Snippet: Ethylene glycol tetraacetic acid (EGTA) was from Amresco.

Article Title: DL0805-2, a novel indazole derivative, relaxes angiotensin II-induced contractions of rat aortic rings by inhibiting Rho kinase and calcium fluxes
Article Snippet: Ethylene glycol tetraacetic acid (EGTA) was purchased from Amresco (Solon, OH, USA).

Article Title: Epigenetic modification and preliminary investigation of the mechanism of the immune evasion of HL-60 cells
Article Snippet: Tween-20, nitrocellulose membrane, Ponceau S solution and ethylene glycol tetraacetic acid (EGTA) were from Amresco (Solon, OH, USA).

Article Title: P2X7 Receptor Activation Induces Reactive Oxygen Species Formation and Cell Death in Murine EOC13 Microglia
Article Snippet: Phenyl-methyl-sulfonyl-fluoride (PMSF), n-dodecyl β -D-maltoside, and ethylene glycol tetraacetic acid (EGTA) were from Amresco (Solon, OH).

Article Title: ATP-binding cassette subfamily B member 1 (ABCB1) and subfamily C member 10 (ABCC10) are not primary resistance factors for cabazitaxel
Article Snippet: Potassium phosphate, ethylene glycol tetraacetic acid (EGTA), and adenosine triphosphate (ATP) were products of AMRESCO (Solon, OH, USA).

Article Title: AID and TET2 co‐operation modulates FANCA expression by active demethylation in diffuse large B cell lymphoma
Article Snippet: Cell pellet was lysed in RIPA buffer [50 mM Tris‐HCl (pH 8.0)], 0·15 M NaCl, 1% Triton X‐100, 0·5% NaDoc, 0·1% sodium dodecyl sulphide (SDS), 1 mM ethylenediamine tetraacetic acid (EDTA), 1 mM ethylene glycol tetraacetic acid (EGTA), 1 m M phenylmethane sulphonyl fluoride (PMSF) (Amresco, Solon, OH, USA) and 1 μg/ml Pepstatin A (Sigma) protease inhibitors.

Isolation:

Article Title: Insulator-based dielectrophoresis of mitochondria a
Article Snippet: .. For muscle isolation buffer, sucrose was purchased from MP Biomedicals (Solon, OH, USA), potassium chloride (KCl) and potassium phosphate (K2 HPO4 ) were from Mallinckrodt (Paris, KY, USA), Tris-HCl was from Teknova (Hollister, CA, USA), ethylene glycol tetraacetic acid (EGTA) was from Amresco (Solon, OH, USA), and bovine serum albumin (BSA) was from Roche (Indianapolis, IN, USA). .. For liver isolation buffer, sucrose and EGTA were purchased from Sigma-Aldrich (St. Louis, MO, USA), 3-(N-morpholino)propanesulfonic acid (MOPS) was from Acros (Geel, Belgium), and Tris was from Fisher (Fair Lawn, NJ, USA).

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    Amresco egta
    Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L <t>BAPTA-AM</t> + 0.5 mmol/L <t>EGTA,</t> n = 5) or introducing a
    Egta, supplied by Amresco, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Amresco
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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    Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L BAPTA-AM + 0.5 mmol/L EGTA, n = 5) or introducing a

    Journal: Neuroscience Bulletin

    Article Title: Biophotons Contribute to Retinal Dark Noise

    doi: 10.1007/s12264-016-0029-6

    Figure Lengend Snippet: Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L BAPTA-AM + 0.5 mmol/L EGTA, n = 5) or introducing a

    Article Snippet: A phosphodiesterase 6 (PDE6) inhibitor (Zaprinast, 100 nmol/L, Sigma, St. Louis, MO), BAPTA-AM (10 μmol/L, Molecular Probes, Eugene, OR), and EGTA (0.5 mmol/L, Amresco, Solon, OH) were initially dissolved in DMSO and then diluted to their final concentrations in ACSF or Ringer’s solution.

    Techniques: Activity Assay

    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

    Journal: Molecules and Cells

    Article Title: Doxorubicin Induces the Persistent Activation of Intracellular Transglutaminase 2 That Protects from Cell Death

    doi: 10.1007/s10059-012-2201-9

    Figure Lengend Snippet: Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

    Article Snippet: To identify the mediators of doxorubicin-induced TG2 activation, the cells were pre-treated for 1 h with 1 mM of N-acetylcysteine (NAC; Sigma), 20 μM BAPTA-AM (Molecular probes), 2 mM EGTA (Amresco), 30 μg/ml transforming growth factor β (TGFβ)-neutralizing antibody (R & D Systems), 10 μg/ml toll-like receptor 2 (TLR2)-neutralizing antibody (R & D Systems) or 0–20 mM caffeine (Sigma) before the doxorubicin treatment.

    Techniques: Activation Assay, Activity Assay

    The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM EGTA, n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM BAPTA-AM+0.5 mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P

    Journal: PLoS ONE

    Article Title: Spatiotemporal Imaging of Glutamate-Induced Biophotonic Activities and Transmission in Neural Circuits

    doi: 10.1371/journal.pone.0085643

    Figure Lengend Snippet: The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM EGTA, n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM BAPTA-AM+0.5 mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P

    Article Snippet: BAPTA-AM (10 µM, Molecular Probes, Eugene, OR, USA), EGTA (0.5 mM, Amresco, Solon, OH, USA) and okadaic acid potassium salt (OA, 200 nM, LC Lab, Woburn, MA, USA) were initially dissolved in DMSO and then diluted to their final concentration in the ACSF.

    Techniques: Mouse Assay