ethylene glycol bis tetraacetic acid egta  (Millipore)


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    Name:
    Ethylene glycol bis
    Description:

    Catalog Number:
    e3257
    Price:
    None
    Applications:
    Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) has been used as a cross-linking agent.
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    Structured Review

    Millipore ethylene glycol bis tetraacetic acid egta
    Ethylene glycol bis

    https://www.bioz.com/result/ethylene glycol bis tetraacetic acid egta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylene glycol bis tetraacetic acid egta - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    phorbol 12-myristate 13-acetate pma

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    Live Cell Imaging:

    Article Title: Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons
    Article Snippet: .. To investigate the effect of calcium buffering on cell migration in the DC electric filed, 1mM of EGTA (Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid, Sigma-Aldrich) was added to each trial just prior to EF application and the start of live-cell imaging. ..

    Protease Inhibitor:

    Article Title: Transcriptional Regulation by ATOH1 and its Target SPDEF in the Intestine
    Article Snippet: .. Chromatin was sheared to 300- to 1000-bp fragments in 1 mL ice-cold sonication buffer (10 mmol/L Tris-HCl pH 8.0, 1 mmol/L EDTA pH 8.0, 1 mmol/L ethylene glycol-bis[β-aminoethyl ether]-N,N,N′,N′ -tetraacetic acid pH 8.0, supplemented with a protease inhibitor cocktail; 539134; Calbiochem), using a 250D Sonifier Ultrasonic Processor Cell Disruptor (Branson, Danbury, CT) with a one-eighth inch microtip (50% power output, interval 1-second on/1-second off, for a total of 24 minutes). .. Sarkosyl was added to a final concentration of 0.5% and the sheared chromatin was incubated at room temperature for 10 minutes and then spun down to remove debris.

    Article Title: Age-Dependent Astroglial Vulnerability to Hypoxia and Glutamate: The Role for Erythropoietin
    Article Snippet: .. The supernatant was removed by aspiration and the pellet was lysed in 100µl of lysis buffer which contained 50mM Hepes, 150mM sodium chloride, 1.5mM magnesium chloride, 100mM sodium fluoride, 10mM tetra-sodium diphosphate decahydrate, 200µM sodium orthovanadate (Merck), 10% Glycerol, 1% Triton X-100,1mM ethylene glycol-bis (2-aminoethylether)-tetra-acetic acid (EGTA, Sigma, Taufkirchen, Germany) and complete Mini protease inhibitor cocktail (La Roche). .. The protein concentration in the samples was determined with the Bio-Rad DcProtein Assay.

    other:

    Article Title: HIRA Is Required for Heart Development and Directly Regulates Tnni2 and Tnnt3
    Article Snippet: HIRA qChIP 30 to 40 E12.5 WT hearts were pooled, washed in PBS and cross-linked for 45 min with 1.5 mM of EGS (Sigma, E3257), followed by 15 min of 1% formaldehyde (from a freshly made filtered stock at 18.5%) at 37°C.

    Article Title: Ca2+-Dependent Contraction by the Saponoside Escin in Rat Vena Cava. Implications in Venotonic Treatment of Varicose Veins
    Article Snippet: For Ca2+ -free Krebs, CaCl2 was omitted and 2 mM ethylene glycolbis[β-aminoethyl ether]-N,N,N',N'-tetraacetic acid (EGTA, Sigma) was added.

    Migration:

    Article Title: Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons
    Article Snippet: .. To investigate the effect of calcium buffering on cell migration in the DC electric filed, 1mM of EGTA (Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid, Sigma-Aldrich) was added to each trial just prior to EF application and the start of live-cell imaging. ..

    Sonication:

    Article Title: Transcriptional Regulation by ATOH1 and its Target SPDEF in the Intestine
    Article Snippet: .. Chromatin was sheared to 300- to 1000-bp fragments in 1 mL ice-cold sonication buffer (10 mmol/L Tris-HCl pH 8.0, 1 mmol/L EDTA pH 8.0, 1 mmol/L ethylene glycol-bis[β-aminoethyl ether]-N,N,N′,N′ -tetraacetic acid pH 8.0, supplemented with a protease inhibitor cocktail; 539134; Calbiochem), using a 250D Sonifier Ultrasonic Processor Cell Disruptor (Branson, Danbury, CT) with a one-eighth inch microtip (50% power output, interval 1-second on/1-second off, for a total of 24 minutes). .. Sarkosyl was added to a final concentration of 0.5% and the sheared chromatin was incubated at room temperature for 10 minutes and then spun down to remove debris.

    Lysis:

    Article Title: Age-Dependent Astroglial Vulnerability to Hypoxia and Glutamate: The Role for Erythropoietin
    Article Snippet: .. The supernatant was removed by aspiration and the pellet was lysed in 100µl of lysis buffer which contained 50mM Hepes, 150mM sodium chloride, 1.5mM magnesium chloride, 100mM sodium fluoride, 10mM tetra-sodium diphosphate decahydrate, 200µM sodium orthovanadate (Merck), 10% Glycerol, 1% Triton X-100,1mM ethylene glycol-bis (2-aminoethylether)-tetra-acetic acid (EGTA, Sigma, Taufkirchen, Germany) and complete Mini protease inhibitor cocktail (La Roche). .. The protein concentration in the samples was determined with the Bio-Rad DcProtein Assay.

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  • 95
    Millipore egta
    2-APB and BAPTA-AM prevent viral-induced FAK phosphorylation. CaSki cells were serum starved overnight, and a synchronized infection with HSV-2(G) was conducted as described in Materials and methods. 100 μM 2-APB, 10 μM <t>nifedipine,</t> or control buffer (5% DMSO) was added to cells at the time of binding or penetration (temperature shift, 15 min, 37°C). Cells were treated with low pH citrate buffer, washed, and cell lysates were prepared 10 min after citrate treatment. Western blots were prepared, probed with anti-FAK pY 397 , and scanned by optical densitometry. Blots were then stripped and reprobed with mAb to total FAK. A representative blot is shown in A. Alternatively, cells were pretreated for 2 h with BAPTA-AM, <t>EGTA,</t> or 5% methanol in PBS (control), and then exposed to HSV-2(G). (B) Lysates were prepared 5 min after infection and analyzed by Western blotting for FAK phosphorylation. (C) Results obtained from three independent experiments are summarized graphically as odu of phosphorylated FAK:odu total FAK as a percentage of control (HSV-2(G) in the absence of any inhibitors). Results are the mean ± SD obtained from three independent experiments; asterisks indicate P
    Egta, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Millipore
    Average 95 stars, based on 1328 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

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    2-APB and BAPTA-AM prevent viral-induced FAK phosphorylation. CaSki cells were serum starved overnight, and a synchronized infection with HSV-2(G) was conducted as described in Materials and methods. 100 μM 2-APB, 10 μM nifedipine, or control buffer (5% DMSO) was added to cells at the time of binding or penetration (temperature shift, 15 min, 37°C). Cells were treated with low pH citrate buffer, washed, and cell lysates were prepared 10 min after citrate treatment. Western blots were prepared, probed with anti-FAK pY 397 , and scanned by optical densitometry. Blots were then stripped and reprobed with mAb to total FAK. A representative blot is shown in A. Alternatively, cells were pretreated for 2 h with BAPTA-AM, EGTA, or 5% methanol in PBS (control), and then exposed to HSV-2(G). (B) Lysates were prepared 5 min after infection and analyzed by Western blotting for FAK phosphorylation. (C) Results obtained from three independent experiments are summarized graphically as odu of phosphorylated FAK:odu total FAK as a percentage of control (HSV-2(G) in the absence of any inhibitors). Results are the mean ± SD obtained from three independent experiments; asterisks indicate P

    Journal: The Journal of Cell Biology

    Article Title: Herpes simplex virus triggers activation of calcium-signaling pathways

    doi: 10.1083/jcb.200301084

    Figure Lengend Snippet: 2-APB and BAPTA-AM prevent viral-induced FAK phosphorylation. CaSki cells were serum starved overnight, and a synchronized infection with HSV-2(G) was conducted as described in Materials and methods. 100 μM 2-APB, 10 μM nifedipine, or control buffer (5% DMSO) was added to cells at the time of binding or penetration (temperature shift, 15 min, 37°C). Cells were treated with low pH citrate buffer, washed, and cell lysates were prepared 10 min after citrate treatment. Western blots were prepared, probed with anti-FAK pY 397 , and scanned by optical densitometry. Blots were then stripped and reprobed with mAb to total FAK. A representative blot is shown in A. Alternatively, cells were pretreated for 2 h with BAPTA-AM, EGTA, or 5% methanol in PBS (control), and then exposed to HSV-2(G). (B) Lysates were prepared 5 min after infection and analyzed by Western blotting for FAK phosphorylation. (C) Results obtained from three independent experiments are summarized graphically as odu of phosphorylated FAK:odu total FAK as a percentage of control (HSV-2(G) in the absence of any inhibitors). Results are the mean ± SD obtained from three independent experiments; asterisks indicate P

    Article Snippet: Reagents Verapamil, nifedipine, 2-APB, Tg, EGTA, EGTA-AM, and ionomycin were purchased from Calbiochem and diluted in DMSO or PBS per manufacturer's instructions.

    Techniques: Infection, Binding Assay, Western Blot

    The ER Ca 2+ release is required for HSV infection. The effects of pretreating cells for 1 h with 50 μM BAPTA-AM, 0.5 mM EGTA, or adding 100 μM 2-APB, 10 μM nifedipine, or 10 μM verapamil during viral penetration on HSV-1(KOS), HSV-2(G), or VSV infection of Vero cells (top) or CaSki cells (bottom) were examined. Results are presented as pfu formed in the presence of the indicated concentration of drug as a percentage of pfu formed in cells treated with control buffer (5% DMSO for pharmacological inhibitors or 5% methanol for BAPTA), and are means of at least three independent experiments conducted in duplicate. Cytotoxicity of pharmacological agents was determined in parallel after a 2-h exposure to drug and quantifying viable, proliferating cells at 24 h by MTS assay. Cell viability results are expressed as odu reading in the presence of the drug as a percentage of odu reading in the presence of control buffer, and are means of two independent experiments conducted in triplicate. Asterisks denote P

    Journal: The Journal of Cell Biology

    Article Title: Herpes simplex virus triggers activation of calcium-signaling pathways

    doi: 10.1083/jcb.200301084

    Figure Lengend Snippet: The ER Ca 2+ release is required for HSV infection. The effects of pretreating cells for 1 h with 50 μM BAPTA-AM, 0.5 mM EGTA, or adding 100 μM 2-APB, 10 μM nifedipine, or 10 μM verapamil during viral penetration on HSV-1(KOS), HSV-2(G), or VSV infection of Vero cells (top) or CaSki cells (bottom) were examined. Results are presented as pfu formed in the presence of the indicated concentration of drug as a percentage of pfu formed in cells treated with control buffer (5% DMSO for pharmacological inhibitors or 5% methanol for BAPTA), and are means of at least three independent experiments conducted in duplicate. Cytotoxicity of pharmacological agents was determined in parallel after a 2-h exposure to drug and quantifying viable, proliferating cells at 24 h by MTS assay. Cell viability results are expressed as odu reading in the presence of the drug as a percentage of odu reading in the presence of control buffer, and are means of two independent experiments conducted in triplicate. Asterisks denote P

    Article Snippet: Reagents Verapamil, nifedipine, 2-APB, Tg, EGTA, EGTA-AM, and ionomycin were purchased from Calbiochem and diluted in DMSO or PBS per manufacturer's instructions.

    Techniques: Infection, Concentration Assay, MTS Assay

    Cleavage of γ c by calpain. In vitro -translated wild-type human γ c ( A ) and γ c in which the PEST sequence was mutated ( B ) were treated with m-calpain and run on 4–20% SDS gels. Although mature γ c is approximately 64 kDa, in vitro -translated γ c migrates at approximately 42 kDa, at least in part due to the lack of glycosylation. We confirmed a report that in vitro ). Murine wild-type and PEST-mutated γ c yielded similar results to those shown for human γ c (data not shown). ( C ) Proteolysis of γ c by calpain in YT cells. YT cell lysates were incubated with 5 mM CaCl 2 (lanes 4, 5, and 7–10) or 10 mM EGTA + 2.5 mM EDTA (lanes 3 and 6) at 37°C for 0–60 min, and reactions were stopped by the addition of 10 mM EGTA/10 μg/ml leupeptin (Boehringer Mannheim)/240 μg/ml 4-[2-aminoethyl]-benzenesulfonyl fluoride hydrochloride/10 μg/ml aprotinin (ICN). In lanes 4, 7, and 10, 20 μM calpastatin or 50 μg/ml antipain also were added. Reactions were stopped with 10 mM EGTA and the protease inhibitor mix. Lysates were immunoprecipitated with chicken anti-human γ c (lanes 2–10) or control chicken IgY (lane 1), and immunoblotted using R878 antiserum to γ c . As R878 antiserum recognizes the C-terminal end of γ c , cleavage in the cytoplasmic domain would result in immunoreactive fragments too small to be retained on these gels. The cleavage was specific, as shown by the lack of general degradation of cellular proteins as detected by Coomassie staining (data not shown).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Functional cleavage of the common cytokine receptor ? chain (?c) by calpain

    doi:

    Figure Lengend Snippet: Cleavage of γ c by calpain. In vitro -translated wild-type human γ c ( A ) and γ c in which the PEST sequence was mutated ( B ) were treated with m-calpain and run on 4–20% SDS gels. Although mature γ c is approximately 64 kDa, in vitro -translated γ c migrates at approximately 42 kDa, at least in part due to the lack of glycosylation. We confirmed a report that in vitro ). Murine wild-type and PEST-mutated γ c yielded similar results to those shown for human γ c (data not shown). ( C ) Proteolysis of γ c by calpain in YT cells. YT cell lysates were incubated with 5 mM CaCl 2 (lanes 4, 5, and 7–10) or 10 mM EGTA + 2.5 mM EDTA (lanes 3 and 6) at 37°C for 0–60 min, and reactions were stopped by the addition of 10 mM EGTA/10 μg/ml leupeptin (Boehringer Mannheim)/240 μg/ml 4-[2-aminoethyl]-benzenesulfonyl fluoride hydrochloride/10 μg/ml aprotinin (ICN). In lanes 4, 7, and 10, 20 μM calpastatin or 50 μg/ml antipain also were added. Reactions were stopped with 10 mM EGTA and the protease inhibitor mix. Lysates were immunoprecipitated with chicken anti-human γ c (lanes 2–10) or control chicken IgY (lane 1), and immunoblotted using R878 antiserum to γ c . As R878 antiserum recognizes the C-terminal end of γ c , cleavage in the cytoplasmic domain would result in immunoreactive fragments too small to be retained on these gels. The cleavage was specific, as shown by the lack of general degradation of cellular proteins as detected by Coomassie staining (data not shown).

    Article Snippet: Lysates were centrifuged at 15,000 rpm for 20 min at 4°C and incubated with CaCl2 (final concentration 5 mM) or EGTA (final concentration 10 mM) at 37°C for 0 to 60 min. To determine the specificity of proteolysis by calpain, calpastatin (20 μM, Calbiochem), an endogenous protease inhibitor that acts specifically on calpain, or antipain [S-(1 carboxy-2-phenylethyl)- l -carbamyl= l -arginyl- l -valylargininal] (Calbiochem), another potent inhibitor of calpain, were incubated in the presence of 5 mM CaCl2 .

    Techniques: In Vitro, Sequencing, Incubation, Protease Inhibitor, Immunoprecipitation, Staining

    In vitro fusion of early endosomes is inhibited by BAPTA and EGTA-AM but not by EGTA. Fusion was performed under standard conditions (see MATERIALS AND METHODS for details). The numbers in parentheses give the numbers of independent experiments. Error bars represent SD.

    Journal: Molecular Biology of the Cell

    Article Title: Fusion of Endosomes Involved in Synaptic Vesicle Recycling

    doi:

    Figure Lengend Snippet: In vitro fusion of early endosomes is inhibited by BAPTA and EGTA-AM but not by EGTA. Fusion was performed under standard conditions (see MATERIALS AND METHODS for details). The numbers in parentheses give the numbers of independent experiments. Error bars represent SD.

    Article Snippet: Stock solutions for 1,2-bis(2-aminophenoxy)ethane- N,N,N′,N′ -tetra-acetic acid (BAPTA; Molecular Probes, Eugene, OR), EGTA, and EGTA-acetoxymethyl ester (AM) (Calbiochem, La Jolla, CA; 200 mM) were carefully adjusted to neutral pH.

    Techniques: In Vitro

    Calcium stores in the endoplasmic reticulum are the main source for spontaneous calcium activity in MM cells. A ) Application of 30 µM CPA caused almost complete inhibition of calcium transients after an initial rise in intracellular calcium. B – E ) Binary representation of spontaneous calcium transients from 8 randomly selected MM cells exposed to 30 µM CPA ( B ), calcium-free solution (250 µM EGTA) ( C ), 50 µM nifedipine ( D ), and 10 µM BTP-2 ( E ). F , G ) Chronic application of 5 µM CPA from DIV1 to DIV2. H ) Comparison of calcium activity in control and CPA-treated kidneys. Box plot represents the relative number of cells that displayed multiple (≥5) calcium spikes in 5 kidneys in both the control and CPA-treated groups. Statistical significance was calculated by 2-tailed, nonparametric U test ( P

    Journal: The FASEB Journal

    Article Title: Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney

    doi: 10.1096/fj.201802054R

    Figure Lengend Snippet: Calcium stores in the endoplasmic reticulum are the main source for spontaneous calcium activity in MM cells. A ) Application of 30 µM CPA caused almost complete inhibition of calcium transients after an initial rise in intracellular calcium. B – E ) Binary representation of spontaneous calcium transients from 8 randomly selected MM cells exposed to 30 µM CPA ( B ), calcium-free solution (250 µM EGTA) ( C ), 50 µM nifedipine ( D ), and 10 µM BTP-2 ( E ). F , G ) Chronic application of 5 µM CPA from DIV1 to DIV2. H ) Comparison of calcium activity in control and CPA-treated kidneys. Box plot represents the relative number of cells that displayed multiple (≥5) calcium spikes in 5 kidneys in both the control and CPA-treated groups. Statistical significance was calculated by 2-tailed, nonparametric U test ( P

    Article Snippet: Chemicals Cyclopiazonic acid (CPA), EGTA, and nifedipine were purchased from MilliporeSigma Sweden AB (Stockholm, Sweden).

    Techniques: Activity Assay, Inhibition