ethylendiaminetetraacetic acid  (Millipore)


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    Structured Review

    Millipore ethylendiaminetetraacetic acid
    Ethylendiaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylendiaminetetraacetic acid/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylendiaminetetraacetic acid - by Bioz Stars, 2020-04
    92/100 stars

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    Centrifugation:

    Article Title: Folylpoly-Glutamate Synthetase Expression is associated with Tumor Response and Outcome from Pemetrexed-based Chemotherapy in Malignant Pleural Mesothelioma
    Article Snippet: Cells were grown until 80% to 95% confluency, then washed and trypsinized with 2 ml of 0.25% trypsin-0.03% ethylendiaminetetraacetic acid (EDTA, Sigma-Aldrich, St. Louis, MO, USA). .. Following centrifugation at 150 × g for 5 minutes cell pellets were washed twice with Phosphate-Buffered Saline (PBS, Mediatech Inc., Manassas, VA, USA) and centrifuged at 150 g for 5 minutes each.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Folylpoly-Glutamate Synthetase Expression is associated with Tumor Response and Outcome from Pemetrexed-based Chemotherapy in Malignant Pleural Mesothelioma
    Article Snippet: Cells were grown until 80% to 95% confluency, then washed and trypsinized with 2 ml of 0.25% trypsin-0.03% ethylendiaminetetraacetic acid (EDTA, Sigma-Aldrich, St. Louis, MO, USA). .. These formalin-fixed paraffin-embedded (FFPE) cell blocks were sectioned at 4 μm thickness and mounted on Plus GOLDR slides.

    Cell Culture:

    Article Title: Folylpoly-Glutamate Synthetase Expression is associated with Tumor Response and Outcome from Pemetrexed-based Chemotherapy in Malignant Pleural Mesothelioma
    Article Snippet: NCI-H28, NCI-H2052, NCI-H2452, MeT-5A, and MSTO-211 were cultured in Roswell Park Memorial Institute (RPMI) medium (Sigma-Aldrich, St. Louis, MO, USA) containing 10% fetal bovine serum and 100 U/ml of penicillin and 100 μg/ml streptomycin (Sigma-Aldrich) at 37°C in a 5% CO2 -humidified atmosphere. .. Cells were grown until 80% to 95% confluency, then washed and trypsinized with 2 ml of 0.25% trypsin-0.03% ethylendiaminetetraacetic acid (EDTA, Sigma-Aldrich, St. Louis, MO, USA).

    Incubation:

    Article Title: Toll-Like Receptor Ligands LPS and Poly (I:C) Exacerbate Airway Hyperresponsiveness in a Model of Airway Allergy in Mice, Independently of Inflammation
    Article Snippet: BAL fluid (BALF) was collected by inserting and excising 1 ml PBS containing 0.6 M ethylendiaminetetraacetic acid (EDTA, Sigma-Aldrich) into the lungs three times. .. To lyse red blood cells, the pellet was incubated with lysis buffer [150 mM NH4 Cl, 10 mM KHCO3 , 0.1 mM EDTA, pH 7.2] for two minutes and subsequently washed in PBS.

    other:

    Article Title: Detection of prion seeding activity in the olfactory mucosa of patients with Fatal Familial Insomnia
    Article Snippet: Substrate preparation for PMCA analysis Bank voles carrying the M109 PrP genotype were sacrificed with carbon dioxide and immediately perfused using phosphate-buffered saline (PBS) plus 5 mM ethylendiaminetetraacetic acid (EDTA, Sigma).

    BIA-KA:

    Article Title: Quercetin Protects Obesity-Induced Hypothalamic Inflammation by Reducing Microglia-Mediated Inflammatory Responses via HO-1 Induction
    Article Snippet: Western Blot Analysis The cells were lysed in lysis buffer (150 mM NaCl, 50 mM Tris-HCl (pH 7.4), 50 mM NaF, 10 mM Na4 P2 O7 , 1 mM Ethylendiaminetetraacetic acid, 1% IGEPAL) supplemented with protease inhibitors cocktail (Sigma). .. Protein concentrations of the lysates were determined by BCA protein assay reagents (Pierce).

    Western Blot:

    Article Title: Quercetin Protects Obesity-Induced Hypothalamic Inflammation by Reducing Microglia-Mediated Inflammatory Responses via HO-1 Induction
    Article Snippet: .. Western Blot Analysis The cells were lysed in lysis buffer (150 mM NaCl, 50 mM Tris-HCl (pH 7.4), 50 mM NaF, 10 mM Na4 P2 O7 , 1 mM Ethylendiaminetetraacetic acid, 1% IGEPAL) supplemented with protease inhibitors cocktail (Sigma). .. Protein concentrations of the lysates were determined by BCA protein assay reagents (Pierce).

    Lysis:

    Article Title: Quercetin Protects Obesity-Induced Hypothalamic Inflammation by Reducing Microglia-Mediated Inflammatory Responses via HO-1 Induction
    Article Snippet: .. Western Blot Analysis The cells were lysed in lysis buffer (150 mM NaCl, 50 mM Tris-HCl (pH 7.4), 50 mM NaF, 10 mM Na4 P2 O7 , 1 mM Ethylendiaminetetraacetic acid, 1% IGEPAL) supplemented with protease inhibitors cocktail (Sigma). .. Protein concentrations of the lysates were determined by BCA protein assay reagents (Pierce).

    Article Title: Toll-Like Receptor Ligands LPS and Poly (I:C) Exacerbate Airway Hyperresponsiveness in a Model of Airway Allergy in Mice, Independently of Inflammation
    Article Snippet: BAL fluid (BALF) was collected by inserting and excising 1 ml PBS containing 0.6 M ethylendiaminetetraacetic acid (EDTA, Sigma-Aldrich) into the lungs three times. .. To lyse red blood cells, the pellet was incubated with lysis buffer [150 mM NH4 Cl, 10 mM KHCO3 , 0.1 mM EDTA, pH 7.2] for two minutes and subsequently washed in PBS.

    Staining:

    Article Title: Toll-Like Receptor Ligands LPS and Poly (I:C) Exacerbate Airway Hyperresponsiveness in a Model of Airway Allergy in Mice, Independently of Inflammation
    Article Snippet: BAL fluid (BALF) was collected by inserting and excising 1 ml PBS containing 0.6 M ethylendiaminetetraacetic acid (EDTA, Sigma-Aldrich) into the lungs three times. .. Differential cell counts were performed on May-Grünwald/Giemsa stained cytospins.

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    Millipore ethylenediaminetetraacetic acid
    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with <t>ethylenediaminetetraacetic</t> acid shows absence of activity. (C)
    Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
    99/100 stars
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    Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Journal: Tissue Engineering. Part A

    Article Title: Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue

    doi: 10.1089/ten.tea.2018.0036

    Figure Lengend Snippet: Matrix metalloproteinase (MMP) gene expression and protein activity. (A) RNA-seq showed MMP2 was not differentially regulated but MMP14 was significantly upregulated. (B) On zymography, bands corresponding to pro MMP2 and active MMP2 were seen. The Std lane corresponds to recombinant MMP2. Inhibition with ethylenediaminetetraacetic acid shows absence of activity. (C)

    Article Snippet: Ethylenediaminetetraacetic acid (15 mM) was used for positive control of MMP inhibition and recombinant MMP2 (MilliporeSigma, Burlington, MA) was used as a positive control.

    Techniques: Expressing, Activity Assay, RNA Sequencing Assay, Zymography, Recombinant, Inhibition