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Decon Laboratories ethanol 200 proof
Ethanol 200 Proof, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethanol 200 proof/product/Decon Laboratories
Average 84 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethanol 200 proof - by Bioz Stars, 2019-10
84/100 stars

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High Performance Liquid Chromatography:

Article Title: Automated synthesis of [11C]L-glutamine on Synthra HCN plus synthesis module
Article Snippet: The standard compounds L-glutamine, D-glutamine, L-glutamic acid, HPLC grade methanol and acetonitrile, 18-crown-6, acetone, trifluoroacetic acid, and concentrated sulfuric acid were purchased from Sigma-Aldrich corporation, USA. .. Ethanol 200 proof was purchased from Decon laboratories Inc. PA, USA.

Negative Control:

Article Title: Combined fluorescent in situ hybridization for detection of microRNAs and immunofluorescent labeling for cell-type markers
Article Snippet: We used snRNA U6 as positive control and a scrambled probe with no complementarity to any known miRNAs as negative control. .. Ethanol 200 proof (Decon Labs Inc., King of Prussia, PA, USA, Catalog # 2701).

Positive Control:

Article Title: Combined fluorescent in situ hybridization for detection of microRNAs and immunofluorescent labeling for cell-type markers
Article Snippet: We used snRNA U6 as positive control and a scrambled probe with no complementarity to any known miRNAs as negative control. .. Ethanol 200 proof (Decon Labs Inc., King of Prussia, PA, USA, Catalog # 2701).

Magnetic Beads:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: Custom oligonucleotide microarrays (Agilent Technologies) Array CGH reagents Microsatellite genotyping reagents Somatic cell hybrid reagents Molecular inversion probes (MIPs, Integrated DNA Technologies) T4 DNA Ligase Buffer with 10 mM ATP (New England BioLabs cat. no. B0202S) T4 polynucleotide kinase (New England BioLabs, cat. no. M0201L) Ampligase buffer (Epicentre, cat. no A1905B) 10 mM dNTP mix (Roche NimbleGen, cat. no. 11581295001) Hemo Klentaq (New England BioLabs, cat. no. M0332L) Ampligase (Epicentre, cat. no. A0110K) Nuclease-free water (Ambion, cat. no. AM9906) Genomic DNA from individuals to be analyzed CAUTION: All human genetic studies must be approved by an institutional review board, and all participating subjects must provide informed consent. .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

Purification:

Article Title: ITO/poly(aniline)/sol-gel glass: An optically transparent, pH-responsive substrate for supported lipid bilayers
Article Snippet: The following chemicals were purchased from Aldrich and used without further purification: tetraethyl orthosilicate (TEOS; 99.999%); triethoxymethylsilane (MTES; 99%); polyaniline (PANI; 10,000 MW emeraldine base); anhydrous 1-methyl-2-pyrrolidinone (NMP; 99.5%). .. Ethanol 200 proof was purchased from Decon Laboratories.

SYBR Green Assay:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: Custom oligonucleotide microarrays (Agilent Technologies) Array CGH reagents Microsatellite genotyping reagents Somatic cell hybrid reagents Molecular inversion probes (MIPs, Integrated DNA Technologies) T4 DNA Ligase Buffer with 10 mM ATP (New England BioLabs cat. no. B0202S) T4 polynucleotide kinase (New England BioLabs, cat. no. M0201L) Ampligase buffer (Epicentre, cat. no A1905B) 10 mM dNTP mix (Roche NimbleGen, cat. no. 11581295001) Hemo Klentaq (New England BioLabs, cat. no. M0332L) Ampligase (Epicentre, cat. no. A0110K) Nuclease-free water (Ambion, cat. no. AM9906) Genomic DNA from individuals to be analyzed CAUTION: All human genetic studies must be approved by an institutional review board, and all participating subjects must provide informed consent. .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

Polymerase Chain Reaction:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: Custom oligonucleotide microarrays (Agilent Technologies) Array CGH reagents Microsatellite genotyping reagents Somatic cell hybrid reagents Molecular inversion probes (MIPs, Integrated DNA Technologies) T4 DNA Ligase Buffer with 10 mM ATP (New England BioLabs cat. no. B0202S) T4 polynucleotide kinase (New England BioLabs, cat. no. M0201L) Ampligase buffer (Epicentre, cat. no A1905B) 10 mM dNTP mix (Roche NimbleGen, cat. no. 11581295001) Hemo Klentaq (New England BioLabs, cat. no. M0332L) Ampligase (Epicentre, cat. no. A0110K) Nuclease-free water (Ambion, cat. no. AM9906) Genomic DNA from individuals to be analyzed CAUTION: All human genetic studies must be approved by an institutional review board, and all participating subjects must provide informed consent. .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

Formalin-fixed Paraffin-Embedded:

Article Title: Combined fluorescent in situ hybridization for detection of microRNAs and immunofluorescent labeling for cell-type markers
Article Snippet: Materials required for deparaffinization, rehydration, and conditioning of FFPE sections: Xylene (Fisher Scientific, Catalog # X5P-1GAL). .. Ethanol 200 proof (Decon Labs Inc., King of Prussia, PA, USA, Catalog # 2701).

Sequencing:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: Custom oligonucleotide microarrays (Agilent Technologies) Array CGH reagents Microsatellite genotyping reagents Somatic cell hybrid reagents Molecular inversion probes (MIPs, Integrated DNA Technologies) T4 DNA Ligase Buffer with 10 mM ATP (New England BioLabs cat. no. B0202S) T4 polynucleotide kinase (New England BioLabs, cat. no. M0201L) Ampligase buffer (Epicentre, cat. no A1905B) 10 mM dNTP mix (Roche NimbleGen, cat. no. 11581295001) Hemo Klentaq (New England BioLabs, cat. no. M0332L) Ampligase (Epicentre, cat. no. A0110K) Nuclease-free water (Ambion, cat. no. AM9906) Genomic DNA from individuals to be analyzed CAUTION: All human genetic studies must be approved by an institutional review board, and all participating subjects must provide informed consent. .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

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    Decon Laboratories 200 proof ethanol
    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: <t>200</t> μm.
    200 Proof Ethanol, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/200 proof ethanol/product/Decon Laboratories
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    200 proof ethanol - by Bioz Stars, 2019-10
    93/100 stars
      Buy from Supplier

    77
    Decon Laboratories bv 200 proof ethanol
    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: <t>200</t> μm.
    Bv 200 Proof Ethanol, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bv 200 proof ethanol/product/Decon Laboratories
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bv 200 proof ethanol - by Bioz Stars, 2019-10
    77/100 stars
      Buy from Supplier

    Image Search Results


    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: 200 μm.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury

    doi: 10.3389/fnmol.2018.00243

    Figure Lengend Snippet: High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: 200 μm.

    Article Snippet: Following end-point ABR measurement, mouse temporal bones were obtained and immersed immediately in a 1:3 (v:v) solution of acetic acid (Sigma Aldrich) and 200 proof ethanol (Decon Laboratories).

    Techniques: Imaging, Mass Spectrometry, Generated