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Decon Laboratories ethanol 200 proof
Ethanol 200 Proof, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethanol 200 proof/product/Decon Laboratories
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethanol 200 proof - by Bioz Stars, 2020-05
88/100 stars

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Related Articles

Magnetic Beads:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

Purification:

Article Title: The supragingival biofilm in early childhood caries: clinical and laboratory protocols and bioinformatics pipelines supporting oral metagenomics, metatranscriptomics and metabolomics studies of the oral microbiome.
Article Snippet: .. Total RNA Purification Kit, 96-Well Format (Norgen Biotek, Corp.; P/N 24304, L/N 591338) which includes: Robust Lysis (RL) buffer (P/N 90055, L/N 589694) Wash Solution A (P/N 90028, L/N 590111) 96-Well Filter Plate (P/N 24304, L/N 591338) 96-Well Collection Plate (P/N 24309) 96-Well Elution Plate (P/N 24310) 96-Well Bead Plate (Norgen Biotek, Corp.; P/N 65700, L/N 591926) kit, which includes: 96-Well Transfer Plate (P/N 65702) 96-Well Bead Plate (P/N 65703, L/N 591924) Beta-Mercaptoethanol (B-ME), CAS [60–24-2] (MP Biomedicals, LLC; P/N 194834, L/N QR13543) Ethanol 200 Proof, Anhydrous, CAS [64–17-5] (Decon Labs, Inc.; P/N 2716, L/N DSP-MD.43) Molecular Biology Grade Water, Sterile, CAS [7732–18-5] (Corning; P/N 46–000-CM, L/N 14917003) .. Illumina bcl2fastq [ ] FastQC [ ] bowtie2 [ ] vsearch [ ] HUMAnN2 [ ] Pathoscope2 [ ] LEfSe [ ] ALDEx2 R package [ ] MetaPhlAn2 [ ] ) ) ) ShotMAP [ ] illustrates basic small equipment (excluding CoolBoxes) and supplies needed for supragingival biofilm collection. illustrates an assembled CoolBox unit with CFT 24 CoolRack and a TruCool Cryogenic vial in place.

Article Title: Measurement of Mitochondrial DNA Release in Response to ER Stress
Article Snippet: .. For extraction (see ) 1.5 ml microcentrifuge tubes (Denville Scientific Inc., catalog number: C2170) Gloves 6 well-plates, tissue culture-treated (Corning, catalog number: 3506) Cell lifter (Biologix Group Limited, catalog number: 70-2180) Murine immortalized bone marrow derived macrophages ( ) Thapsigargin, 97%, ACROS Organics™ (Thermo Fisher Scientific, Fisher Scientific™, catalog number: AC328570010) ( ) 1% NP-40 (Igepal CA-630) (Sigma-Aldrich, catalog number: I8896) ( ) DPBS (Thermo Fisher Scientific, Gibco™, catalog number: 14040-133) ( ) DMEM (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (FBS) (Thermo Fisher Scientific, Gibco™, catalog number: 10437-028) Medium (see Recipes) NP-40 (Igepal CA-630) solution (see Recipes) Thapsigargin stock solution (see Recipes) For purification Gloves DNeasy blood & tissue kit, 50 samples (QIAGEN, catalog number: 69504) or 250 samples (QIAGEN, catalog number: 69506) Ethanol 200 proof (Decon Labs, catalog number: 2716) .. Centrifuge (Thermo Fisher Scientific, Fisher Scientific™, model: accuSpin™ Micro 17)

SYBR Green Assay:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

Polymerase Chain Reaction:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

other:

Article Title: High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
Article Snippet: NaOH (Sigma-Aldrich, catalog number: 415413–1L) Ethanol 200 Proof (Decon Labs, catalog number: UN1170) Acrylamide (Sigma-Aldrich, catalog number: A3553–100G) Bis-acrylamide (Sigma-Aldrich, catalog number: M7279–25G) Irgacure 2959 (BASF Corporation, catalog number: 55047962) Methanol (Sigma-Aldrich, catalog number: 179957–1L) Sodium acetate (Sigma-Aldrich, catalog number: S2889–250G) Ethylenediaminetetraacetic (EDTA) (Sigma-Aldrich, catalog number: ED-100G) Glycerol (Sigma-Aldrich, catalog number: M6145–25ML) Glacial acetic acid (Sigma-Aldrich, catalog number: 695092–500ML) Phosphate-buffered saline (PBS) (Fisher Scientific, catalog number: SH3001302) 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich, catalog number: C3023–1G) Dextran-rhodamine (Life Technologies, catalog number: D-1841) 100x penicillin-streptomycin solution (Fisher Scientific , catalog number: SV30010) Bovine serum albumin (Sigma-Aldrich, catalog number: A2153–100G) Sodium dodecyl sulfate (Fisher Scientific, catalog number: BP166–100) Triton X-100 (Sigma-Aldrich, catalog number: X100–1L) 2x ECMP Printing Buffer (see ) 2x Growth Factor (GF) Printing Buffer (see ) Polyacrylamide Prepolymer Solution (see )

Sequencing:

Article Title: Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencing
Article Snippet: .. Exonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, , Operon) SYBR green (Life Technologies cat. no. S-7563, dilute from 10,000X to 100X in DMSO) Reverse barcode primers (10 μM, , Integrated DNA Technologies) Magnetic beads (Agencourt, Beckman Coulter, cat. no. ) Ethanol 200 proof (Decon Laboratories, Inc., cat. no. 2716) Deionized water (MilliQ) Elution buffer (Qiagen, cat. no. 19086) Sequencing primers (100 μM, , Operon) HiSeq or MiSeq kit (Illumina, v2 PE 300 cycles) .. 1.7 mL Eppendorf tube incubators (VWR) Hybridization oven (Shel Lab) Microarray scanner (Agilent technologies) Agilent feature extraction software Microsatellite genotyping equipment Somatic cell hybrid equipment SPARC computer Microsoft Excel Microcentrifuge (Eppendorf) 1.7 mL PCR tube mini centrifuge (Fisher Scientific) 96-well cold blocks (Eppendorf, cat. no. Z606634) Lightcycler (Bio Rad) Optical qPCR tubes, 8-strip (Bio Rad, cat. no. TLS-0851) Optical qPCR caps, 8-strip (Bio Rad, cat. no. TCS-0803) 200 μL 8-strip PCR tube mini centrifuge (Fisher Scientific) Magnet tube rack (Life Technologies MagnaRack, cat. no. CS15000) eGel system (Life Technologies, cat. no. G6512ST) Qubit DNA quantification system (cat. no. ) Illumina MiSeq, HiSeq2000, or HiSeq 2500 sequencer Hardware (64-bit computer running Linux with at least 5 GB RAM—a high-memory, multicore computer is best) Software (see ) Custom analysis programs.

Lysis:

Article Title: The supragingival biofilm in early childhood caries: clinical and laboratory protocols and bioinformatics pipelines supporting oral metagenomics, metatranscriptomics and metabolomics studies of the oral microbiome.
Article Snippet: .. Total RNA Purification Kit, 96-Well Format (Norgen Biotek, Corp.; P/N 24304, L/N 591338) which includes: Robust Lysis (RL) buffer (P/N 90055, L/N 589694) Wash Solution A (P/N 90028, L/N 590111) 96-Well Filter Plate (P/N 24304, L/N 591338) 96-Well Collection Plate (P/N 24309) 96-Well Elution Plate (P/N 24310) 96-Well Bead Plate (Norgen Biotek, Corp.; P/N 65700, L/N 591926) kit, which includes: 96-Well Transfer Plate (P/N 65702) 96-Well Bead Plate (P/N 65703, L/N 591924) Beta-Mercaptoethanol (B-ME), CAS [60–24-2] (MP Biomedicals, LLC; P/N 194834, L/N QR13543) Ethanol 200 Proof, Anhydrous, CAS [64–17-5] (Decon Labs, Inc.; P/N 2716, L/N DSP-MD.43) Molecular Biology Grade Water, Sterile, CAS [7732–18-5] (Corning; P/N 46–000-CM, L/N 14917003) .. Illumina bcl2fastq [ ] FastQC [ ] bowtie2 [ ] vsearch [ ] HUMAnN2 [ ] Pathoscope2 [ ] LEfSe [ ] ALDEx2 R package [ ] MetaPhlAn2 [ ] ) ) ) ShotMAP [ ] illustrates basic small equipment (excluding CoolBoxes) and supplies needed for supragingival biofilm collection. illustrates an assembled CoolBox unit with CFT 24 CoolRack and a TruCool Cryogenic vial in place.

Derivative Assay:

Article Title: Measurement of Mitochondrial DNA Release in Response to ER Stress
Article Snippet: .. For extraction (see ) 1.5 ml microcentrifuge tubes (Denville Scientific Inc., catalog number: C2170) Gloves 6 well-plates, tissue culture-treated (Corning, catalog number: 3506) Cell lifter (Biologix Group Limited, catalog number: 70-2180) Murine immortalized bone marrow derived macrophages ( ) Thapsigargin, 97%, ACROS Organics™ (Thermo Fisher Scientific, Fisher Scientific™, catalog number: AC328570010) ( ) 1% NP-40 (Igepal CA-630) (Sigma-Aldrich, catalog number: I8896) ( ) DPBS (Thermo Fisher Scientific, Gibco™, catalog number: 14040-133) ( ) DMEM (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (FBS) (Thermo Fisher Scientific, Gibco™, catalog number: 10437-028) Medium (see Recipes) NP-40 (Igepal CA-630) solution (see Recipes) Thapsigargin stock solution (see Recipes) For purification Gloves DNeasy blood & tissue kit, 50 samples (QIAGEN, catalog number: 69504) or 250 samples (QIAGEN, catalog number: 69506) Ethanol 200 proof (Decon Labs, catalog number: 2716) .. Centrifuge (Thermo Fisher Scientific, Fisher Scientific™, model: accuSpin™ Micro 17)

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    Decon Laboratories 200 proof ethanol
    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: <t>200</t> μm.
    200 Proof Ethanol, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/200 proof ethanol/product/Decon Laboratories
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    200 proof ethanol - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    Decon Laboratories ethanol
    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: <t>200</t> μm.
    Ethanol, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethanol/product/Decon Laboratories
    Average 92 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    ethanol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: 200 μm.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury

    doi: 10.3389/fnmol.2018.00243

    Figure Lengend Snippet: High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: 200 μm.

    Article Snippet: Following end-point ABR measurement, mouse temporal bones were obtained and immersed immediately in a 1:3 (v:v) solution of acetic acid (Sigma Aldrich) and 200 proof ethanol (Decon Laboratories).

    Techniques: Imaging, Mass Spectrometry, Generated

    High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: 200 μm.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury

    doi: 10.3389/fnmol.2018.00243

    Figure Lengend Snippet: High resolution imaging mass spectrometry in mouse cochlear sections. Two-dimensional ion density map overlay of mass-to-charge ratios 5458 (A) , 6197 (B) , and 11356 (C) in the middle turn of control, 106 or 112 dB noise exposed, and ouabain exposed cochlea. The protein signal at m/z 5458 (A) is present in the lateral wall (LW, arrowhead) of the 112 dB ear with even higher density observed in the LW (arrowhead) of the ouabain exposed cochlea, while showing lower signal presence in the control and 106 dB middle turns. Similarly, the signal at m/z 6197 (B) displays increasing density corresponding to the severity of the cochlear insult, with localization to auditory nerve (asterisk) and Reisner's membrane (arrow). Interestingly, the m/z 11356 (C) demonstrates localization of protein signal in only the ouabain exposed ear. Images were generated at 10–15 μm spatial resolution. Scale bar: 200 μm.

    Article Snippet: Following end-point ABR measurement, mouse temporal bones were obtained and immersed immediately in a 1:3 (v:v) solution of acetic acid (Sigma Aldrich) and 200 proof ethanol (Decon Laboratories).

    Techniques: Imaging, Mass Spectrometry, Generated