Journal: PLoS ONE

Article Title: Therapeutic Hypothermia Activates the Endothelin and Nitric Oxide Systems after Cardiac Arrest in a Pig Model of Cardiopulmonary Resuscitation

doi: 10.1371/journal.pone.0064792

Figure Lengend Snippet: Endothelin A receptor, ETAR (A), and endothelin B receptor, ETBR (B), mRNA expression analysis was performed by real-time qPCR. The relative fold changes to control animals with untreated cardiac arrest (C group) were normalized with β-actin and calculated for animals after 30, 60 and 180 min of untreated return of spontaneous circulation (ROSC30, ROSC60 and ROSC180 groups) or after 180 min of mild therapeutic hypothermia (MTH group). Error bars represent standard error of the mean (SEM). Significant results (p<0.001) are marked with (***).

Article Snippet: The membranes were blocked with SuperBlock® T20 PBS (Thermo Scientific) before incubation in phosphate-buffered saline–Tween-20 with anti-ETBR (1∶200, AER-001; Alomone Labs, Jerusalem, Israel), anti-ETAR (1∶200, AER-002; Alomone Labs), anti-β-actin (1∶200, Sigma), anti-nNOS (1∶3000; Euro-Diagnostica AB, Malmö, Sweden), anti-iNOS (1∶3000; Abcam, Cambridge, MA) and anti-eNOS (1∶200; Santa Cruz Biotechnology, Santa Cruz, CA), respectively.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Therapeutic Hypothermia Activates the Endothelin and Nitric Oxide Systems after Cardiac Arrest in a Pig Model of Cardiopulmonary Resuscitation

doi: 10.1371/journal.pone.0064792

Figure Lengend Snippet: Endothelin 1, ET-1 (A), endothelin converting enzyme 1, ECE-1 (B), endothelin A receptor, ETAR (C), and endothelin B receptor, ETBR (D), mRNA expression analysis was performed by real-time qPCR. The relative fold changes to control animals with untreated cardiac arrest (C group) were normalized with β-actin and calculated for animals after 180 min of untreated return of spontaneous circulation (ROSC180 group) or 180 min after S-PBN infusion (S-PBN group). Error bars represent standard error of the mean (SEM). Significant results (p<0.01) are marked with (**), (p<0.001) are marked with (***).

Article Snippet: The membranes were blocked with SuperBlock® T20 PBS (Thermo Scientific) before incubation in phosphate-buffered saline–Tween-20 with anti-ETBR (1∶200, AER-001; Alomone Labs, Jerusalem, Israel), anti-ETAR (1∶200, AER-002; Alomone Labs), anti-β-actin (1∶200, Sigma), anti-nNOS (1∶3000; Euro-Diagnostica AB, Malmö, Sweden), anti-iNOS (1∶3000; Abcam, Cambridge, MA) and anti-eNOS (1∶200; Santa Cruz Biotechnology, Santa Cruz, CA), respectively.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Therapeutic Hypothermia Activates the Endothelin and Nitric Oxide Systems after Cardiac Arrest in a Pig Model of Cardiopulmonary Resuscitation

doi: 10.1371/journal.pone.0064792

Figure Lengend Snippet: Cardiac left ventricle tissue homogenates from control animals with untreated cardiac arrest (C group), or after 180 min of untreated return of spontaneous circulation (ROSC180 group), after 180 min of mild therapeutic hypothermia (MTH group) or 180 min after S-PBN infusion (S-PBN group) were loaded on a 12% SDS-acrylamide gel. Bands for endothelin A receptor (ETAR), endothelin B receptor (ETBR) and β-actin were detected at 25 kDa, 50 kDa and 42 kDa, respectively (A). Protein band intensities were quantified using a ChemiDoc XRS and Quantity One software and normalized to the loading control (β-actin) (B). Error bars represent standard error of the mean (SEM). Significant results (p<0.05) are marked with (*), (p<0.001) are marked with (***).

Article Snippet: The membranes were blocked with SuperBlock® T20 PBS (Thermo Scientific) before incubation in phosphate-buffered saline–Tween-20 with anti-ETBR (1∶200, AER-001; Alomone Labs, Jerusalem, Israel), anti-ETAR (1∶200, AER-002; Alomone Labs), anti-β-actin (1∶200, Sigma), anti-nNOS (1∶3000; Euro-Diagnostica AB, Malmö, Sweden), anti-iNOS (1∶3000; Abcam, Cambridge, MA) and anti-eNOS (1∶200; Santa Cruz Biotechnology, Santa Cruz, CA), respectively.

Techniques: Acrylamide Gel Assay, Software