escherichia coli strains dh5α  (Thermo Fisher)


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    Thermo Fisher escherichia coli strains dh5α
    Escherichia Coli Strains Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains dh5α/product/Thermo Fisher
    Average 91 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains dh5α - by Bioz Stars, 2020-01
    91/100 stars

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    Clone Assay:

    Article Title: Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
    Article Snippet: .. The E. coli strain DH5α (GIBCO/BRL) was used as a cloning host and to assess the effects of the malE - sulA fusions. .. The malE - sulA fusions were constructed in the expression vector pBAD18 ( ).

    Article Title: Molecular Characterization of Inulosucrase from Leuconostoc citreum: a Fructosyltransferase within a Glucosyltransferase
    Article Snippet: .. A PCR product of 5,007 bp was amplified and cloned with the pCR4-TOPO vector in E. coli strain DH5α (Invitrogen, Carlsbad, Calif.). .. The correct construction of the plasmid was confirmed by sequence analysis of single DNA strands from the insert.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The PCR product was digested with EcoRI and HindIII resulting in a 350 bp internal fragment that was gel purified and cloned into the vector pVA981-2, which replicates episomally in E. coli but not in S. pyogenes . .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315.

    Article Title: Biochemical Characterization and Physiological Properties of Escherichia coli UDP-N-Acetylmuramate:l-Alanyl-?-d-Glutamyl-meso- Diaminopimelate Ligase ▿
    Article Snippet: E. coli strain DH5α (Life Technologies, Inc.) was used as the host for plasmids and for overproduction of the Mpl enzyme. .. Plasmid pAM1005 carrying the murC gene cloned into the expression vector p Trc 99A has been described previously ( ).

    Article Title: Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
    Article Snippet: .. E. coli strain DH5α (Invitrogen, http://www.invitrogen.com ) was used as a cloning host. .. Unfed I. scapularis nymphs were obtained from a colony of I. scapularis ticks maintained by Dr. Durland Fish and Dr. Fred S. Kantor at Yale University.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The PCR product was digested with EcoRI and HindIII resulting in a 350 bp internal fragment that was gel purified and cloned into the vector pVA981-2, which replicates episomally in E. coli but not in S. pyogenes . .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315.

    Amplification:

    Article Title: Molecular Characterization of Inulosucrase from Leuconostoc citreum: a Fructosyltransferase within a Glucosyltransferase
    Article Snippet: .. A PCR product of 5,007 bp was amplified and cloned with the pCR4-TOPO vector in E. coli strain DH5α (Invitrogen, Carlsbad, Calif.). .. The correct construction of the plasmid was confirmed by sequence analysis of single DNA strands from the insert.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The prtF.2 gene was amplified using the primers PrtF2_F1 (5′-GCTTGAATTCAAACGACAGTTCCGGCTGAT-3′) and PrtF2_R1 (5′-GCTTAAGCTTTCAGGTCATCCACTGCTAC-3′). .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The prtF.2 gene was amplified using the primers PrtF2_F1 (5′-GCTTGAATTCAAACGACAGTTCCGGCTGAT-3′) and PrtF2_R1 (5′-GCTTAAGCTTTCAGGTCATCCACTGCTAC-3′). .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315.

    Cytometry:

    Article Title: Substrate Profiling of Tobacco Etch Virus Protease Using a Novel Fluorescence-Assisted Whole-Cell Assay
    Article Snippet: .. E. coli strain DH5α (Gibco) was used for flow cytometry analysis and cell sorting. .. E. coli strain Rosetta (DE3)pLysS (Novagen) was used for production of ABP-PS-ZZ and TEVp.

    Construct:

    Article Title: Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
    Article Snippet: The E. coli strain DH5α (GIBCO/BRL) was used as a cloning host and to assess the effects of the malE - sulA fusions. .. The malE - sulA fusions were constructed in the expression vector pBAD18 ( ).

    Article Title: Overexpression of wbkF gene in Brucella abortus RB51WboA leads to increased O-polysaccharide expression and enhanced vaccine efficacy against B. abortus 2308, B. melitensis 16M, and B. suis 1330 in a murine brucellosis model
    Article Snippet: .. The plasmid constructs were prepared using Escherichia coli strain DH5α (Invitrogen, Carlsbad, CA). ..

    Expressing:

    Article Title: Structural properties of a haemophore facilitate targeted elimination of the pathogen Porphyromonas gingivalis
    Article Snippet: .. Escherichia coli strains DH5α (Invitrogen) and RosettaTM DE3 (Novagen) were used for plasmid construction and recombinant protein expression. ..

    Article Title: Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
    Article Snippet: The E. coli strain DH5α (GIBCO/BRL) was used as a cloning host and to assess the effects of the malE - sulA fusions. .. The malE - sulA fusions were constructed in the expression vector pBAD18 ( ).

    Article Title: Molecular Characterization of Inulosucrase from Leuconostoc citreum: a Fructosyltransferase within a Glucosyltransferase
    Article Snippet: Paragraph title: Construction of plasmids for expression in E. coli of the islA gene and the truncated versions. ... A PCR product of 5,007 bp was amplified and cloned with the pCR4-TOPO vector in E. coli strain DH5α (Invitrogen, Carlsbad, Calif.).

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315. .. The prtF.2 mutant strain was complemented with the shuttle plasmid pAH1, which was derived from the streptococcal expression plasmid pMNN23 .

    Article Title: Biochemical Characterization and Physiological Properties of Escherichia coli UDP-N-Acetylmuramate:l-Alanyl-?-d-Glutamyl-meso- Diaminopimelate Ligase ▿
    Article Snippet: E. coli strain DH5α (Life Technologies, Inc.) was used as the host for plasmids and for overproduction of the Mpl enzyme. .. Plasmid pAM1005 carrying the murC gene cloned into the expression vector p Trc 99A has been described previously ( ).

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315. .. The prtF.2 mutant strain was complemented with the shuttle plasmid pAH1, which was derived from the streptococcal expression plasmid pMNN23 .

    Article Title: Functional Characterization of SsaE, a Novel Chaperone Protein of the Type III Secretion System Encoded by Salmonella Pathogenicity Island 2 ▿
    Article Snippet: .. E. coli strains DH5α (Gibco BRL) and MC1061 ( ) were used for molecular cloning and expression of recombinant proteins. .. E. coli strain S17.1λ pir ( ) was used for propagation of π-dependent plasmids and for conjugation.

    Transformation Assay:

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Derivative Assay:

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315. .. The prtF.2 mutant strain was complemented with the shuttle plasmid pAH1, which was derived from the streptococcal expression plasmid pMNN23 .

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315. .. The prtF.2 mutant strain was complemented with the shuttle plasmid pAH1, which was derived from the streptococcal expression plasmid pMNN23 .

    Conjugation Assay:

    Article Title: Functional Characterization of SsaE, a Novel Chaperone Protein of the Type III Secretion System Encoded by Salmonella Pathogenicity Island 2 ▿
    Article Snippet: E. coli strains DH5α (Gibco BRL) and MC1061 ( ) were used for molecular cloning and expression of recombinant proteins. .. E. coli strain S17.1λ pir ( ) was used for propagation of π-dependent plasmids and for conjugation.

    Flow Cytometry:

    Article Title: Substrate Profiling of Tobacco Etch Virus Protease Using a Novel Fluorescence-Assisted Whole-Cell Assay
    Article Snippet: .. E. coli strain DH5α (Gibco) was used for flow cytometry analysis and cell sorting. .. E. coli strain Rosetta (DE3)pLysS (Novagen) was used for production of ABP-PS-ZZ and TEVp.

    Polymerase Chain Reaction:

    Article Title: Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
    Article Snippet: The E. coli strain DH5α (GIBCO/BRL) was used as a cloning host and to assess the effects of the malE - sulA fusions. .. First, the malE gene was obtained from pMalC (New England Biolabs) by PCR and inserted into the Nhe I and Hin dIII sites of pBAD18 to give pJC90.

    Article Title: Molecular Characterization of Inulosucrase from Leuconostoc citreum: a Fructosyltransferase within a Glucosyltransferase
    Article Snippet: .. A PCR product of 5,007 bp was amplified and cloned with the pCR4-TOPO vector in E. coli strain DH5α (Invitrogen, Carlsbad, Calif.). .. The correct construction of the plasmid was confirmed by sequence analysis of single DNA strands from the insert.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The PCR product was digested with EcoRI and HindIII resulting in a 350 bp internal fragment that was gel purified and cloned into the vector pVA981-2, which replicates episomally in E. coli but not in S. pyogenes . .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The PCR product was digested with EcoRI and HindIII resulting in a 350 bp internal fragment that was gel purified and cloned into the vector pVA981-2, which replicates episomally in E. coli but not in S. pyogenes . .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315.

    Recombinant:

    Article Title: Structural properties of a haemophore facilitate targeted elimination of the pathogen Porphyromonas gingivalis
    Article Snippet: .. Escherichia coli strains DH5α (Invitrogen) and RosettaTM DE3 (Novagen) were used for plasmid construction and recombinant protein expression. ..

    Article Title: An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains
    Article Snippet: Bacterial strains Escherichia coli strain RR1∆M15 [ ] and E. coli strain DH5α (Invitrogen, Carlsbad, CA) were used for subcloning work. .. E. coli DH5α (Invitrogen) was used for OmpT-mediated release of recombinant protein into the medium.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Article Title: Functional Characterization of SsaE, a Novel Chaperone Protein of the Type III Secretion System Encoded by Salmonella Pathogenicity Island 2 ▿
    Article Snippet: .. E. coli strains DH5α (Gibco BRL) and MC1061 ( ) were used for molecular cloning and expression of recombinant proteins. .. E. coli strain S17.1λ pir ( ) was used for propagation of π-dependent plasmids and for conjugation.

    Molecular Cloning:

    Article Title: Functional Characterization of SsaE, a Novel Chaperone Protein of the Type III Secretion System Encoded by Salmonella Pathogenicity Island 2 ▿
    Article Snippet: .. E. coli strains DH5α (Gibco BRL) and MC1061 ( ) were used for molecular cloning and expression of recombinant proteins. .. E. coli strain S17.1λ pir ( ) was used for propagation of π-dependent plasmids and for conjugation.

    Mutagenesis:

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: When appropriate, the prtF.2 mutant, or complemented mutant strains, were grown with THY containing erythromycin (2.5 μg/ml) or kanamycin (500 μg/ml), respectively. .. Escherichia coli strain DH5α (Gibco; Gaithersburg, MD) was grown with Luria Bertani (LB) medium.

    Article Title: Structural properties of a haemophore facilitate targeted elimination of the pathogen Porphyromonas gingivalis
    Article Snippet: Bacterial strains and cultures and cell lines P. gingivalis wild-type W83 and mutant derivatives were grown in enriched Tryptic Soy Broth (eTSB) supplemented with 0.5 g L–1 of l -cysteine, 2 mg L–1 of menadione, and haemin or haemoglobin where indicated at 37 °C in an anaerobic chamber (Don Whitley Scientific Limited, UK) with an atmosphere of 80% N2 , 10% CO2 and 10% H2 . .. Escherichia coli strains DH5α (Invitrogen) and RosettaTM DE3 (Novagen) were used for plasmid construction and recombinant protein expression.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: Paragraph title: GAS mutagenesis ... Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315.

    Article Title: Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
    Article Snippet: This B. burgdorferi isolate, its ospB deletion mutant, and the OspB complemented mutant (ospB − /pFGN1) were cultivated in vitro at 33 °C in Barbour-Stoenner-Kelly complete media (BSK-H, Sigma, http://www.sigmaaldrich.com ). .. E. coli strain DH5α (Invitrogen, http://www.invitrogen.com ) was used as a cloning host.

    Article Title: Functional Characterization of SsaE, a Novel Chaperone Protein of the Type III Secretion System Encoded by Salmonella Pathogenicity Island 2 ▿
    Article Snippet: In this study, S. enterica serovar Typhimurium strain SL1344 was used as the wild-type strain, and strains harboring an ssaV mutation and an spiA ( C133S ) point mutation were used as general SPI-2 T3SS component mutants. .. E. coli strains DH5α (Gibco BRL) and MC1061 ( ) were used for molecular cloning and expression of recombinant proteins.

    Isolation:

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Article Title: Viral Evasion of a Bacterial Suicide System by RNA-Based Molecular Mimicry Enables Infectious Altruism
    Article Snippet: All experiments were performed with E. coli strain DH5α (Gibco/BRL) or Pectobacterium atrosepticum SCRI1043 and derivatives thereof. .. Bacteriophage ΦTE was isolated from sewage effluent collected from the outlet at Milton sewage treatment plant, near Cambridge, UK.

    Subcloning:

    Article Title: An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains
    Article Snippet: .. Bacterial strains Escherichia coli strain RR1∆M15 [ ] and E. coli strain DH5α (Invitrogen, Carlsbad, CA) were used for subcloning work. .. E. coli strain BL21 (DE3) (Merck, Darmstadt, Germany) was used for cell surface display.

    Purification:

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The PCR product was digested with EcoRI and HindIII resulting in a 350 bp internal fragment that was gel purified and cloned into the vector pVA981-2, which replicates episomally in E. coli but not in S. pyogenes . .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: The PCR product was digested with EcoRI and HindIII resulting in a 350 bp internal fragment that was gel purified and cloned into the vector pVA981-2, which replicates episomally in E. coli but not in S. pyogenes . .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315.

    Sequencing:

    Article Title: Molecular Characterization of Inulosucrase from Leuconostoc citreum: a Fructosyltransferase within a Glucosyltransferase
    Article Snippet: A PCR product of 5,007 bp was amplified and cloned with the pCR4-TOPO vector in E. coli strain DH5α (Invitrogen, Carlsbad, Calif.). .. The correct construction of the plasmid was confirmed by sequence analysis of single DNA strands from the insert.

    Plasmid Preparation:

    Article Title: Structural properties of a haemophore facilitate targeted elimination of the pathogen Porphyromonas gingivalis
    Article Snippet: .. Escherichia coli strains DH5α (Invitrogen) and RosettaTM DE3 (Novagen) were used for plasmid construction and recombinant protein expression. ..

    Article Title: Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
    Article Snippet: The E. coli strain DH5α (GIBCO/BRL) was used as a cloning host and to assess the effects of the malE - sulA fusions. .. The malE - sulA fusions were constructed in the expression vector pBAD18 ( ).

    Article Title: Molecular Characterization of Inulosucrase from Leuconostoc citreum: a Fructosyltransferase within a Glucosyltransferase
    Article Snippet: .. A PCR product of 5,007 bp was amplified and cloned with the pCR4-TOPO vector in E. coli strain DH5α (Invitrogen, Carlsbad, Calif.). .. The correct construction of the plasmid was confirmed by sequence analysis of single DNA strands from the insert.

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2 ::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Article Title: Overexpression of wbkF gene in Brucella abortus RB51WboA leads to increased O-polysaccharide expression and enhanced vaccine efficacy against B. abortus 2308, B. melitensis 16M, and B. suis 1330 in a murine brucellosis model
    Article Snippet: .. The plasmid constructs were prepared using Escherichia coli strain DH5α (Invitrogen, Carlsbad, CA). ..

    Article Title: Biochemical Characterization and Physiological Properties of Escherichia coli UDP-N-Acetylmuramate:l-Alanyl-?-d-Glutamyl-meso- Diaminopimelate Ligase ▿
    Article Snippet: E. coli strain DH5α (Life Technologies, Inc.) was used as the host for plasmids and for overproduction of the Mpl enzyme. .. Plasmid pAM1005 carrying the murC gene cloned into the expression vector p Trc 99A has been described previously ( ).

    Article Title: The Streptococcus pyogenes fibronectin/tenascin-binding protein PrtF.2 contributes to virulence in an influenza superinfection
    Article Snippet: .. Following transformation of E. coli strain DH5α (Gibco-BRL, USA), the recombinant plasmid, designated pVA981-2::prtF.2 , was isolated and electroporated into strain MGAS315. .. Transformants were selected with THY agar plates containing erythromycin (2.5 μg/ml) and insertional inactivation of prtF.2 was verified with PCR.

    Functional Assay:

    Article Title: Identification and classification of bacterial Type III toxin-antitoxin systems encoded in chromosomal and plasmid genomes
    Article Snippet: .. Bacterial strains All functional assays were performed within E. coli strain DH5α (Gibco/BRL). ..

    In Vitro:

    Article Title: Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
    Article Snippet: Spirochetes from in vitro cultures were needle-inoculated (intradermally) at a dose of 105 spirochetes/mouse and were recovered from cultures of ear punch biopsies at 2 wk after inoculation. .. E. coli strain DH5α (Invitrogen, http://www.invitrogen.com ) was used as a cloning host.

    Concentration Assay:

    Article Title: Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
    Article Snippet: Where necessary, antibiotics kanamycin and streptomycin were added to a final concentration of 350 μg/ml and 50 μg/ml, respectively. .. E. coli strain DH5α (Invitrogen, http://www.invitrogen.com ) was used as a cloning host.

    FACS:

    Article Title: Substrate Profiling of Tobacco Etch Virus Protease Using a Novel Fluorescence-Assisted Whole-Cell Assay
    Article Snippet: .. E. coli strain DH5α (Gibco) was used for flow cytometry analysis and cell sorting. .. E. coli strain Rosetta (DE3)pLysS (Novagen) was used for production of ABP-PS-ZZ and TEVp.

    Fluorescence In Situ Hybridization:

    Article Title: Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
    Article Snippet: E. coli strain DH5α (Invitrogen, http://www.invitrogen.com ) was used as a cloning host. .. Unfed I. scapularis nymphs were obtained from a colony of I. scapularis ticks maintained by Dr. Durland Fish and Dr. Fred S. Kantor at Yale University.

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  • 90
    Thermo Fisher max efficiency dh5α competent cells
    Max Efficiency Dh5α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/max efficiency dh5α competent cells/product/Thermo Fisher
    Average 90 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    max efficiency dh5α competent cells - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher library efficiency dh5α competent cells
    Library Efficiency Dh5α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/library efficiency dh5α competent cells/product/Thermo Fisher
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    library efficiency dh5α competent cells - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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