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Merck KGaA escherichia coli strain bl21
Escherichia Coli Strain Bl21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/escherichia coli strain bl21/product/Merck KGaA
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
escherichia coli strain bl21 - by Bioz Stars, 2020-08
91/100 stars

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Purification:

Article Title: Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana
Article Snippet: .. Expression and purification of the WT and mutated His-tagged ACA8 N-terminus Vectors coding for WT and mutated His-tagged ACA8 N-terminus (6His-1 M-I116 ) were used to transform E. coli strain BL21(DE3)pLysE (Merck KGaA, Darmstadt, Germany, catalogue no. 69389-3) by standard procedures. ..

Produced:

Article Title: The mechanism of NDM-1-catalyzed carbapenem hydrolysis is distinct from that of penicillin or cephalosporin hydrolysis
Article Snippet: .. The recombinant protein with an N-terminal His6 -tag was produced in Escherichia coli strain BL21(DE3) (Merck, Germany) at 22 °C with an incubation for 16–20 h after induction with 0.5 mM isopropyl β-d -1-thiogalactopyranoside (IPTG). .. Harvested cells were resuspended in the lysis buffer containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM imidazole, 10 mM β-mercaptoethanol, and 1 mM ZnCl2 .

Incubation:

Article Title: The mechanism of NDM-1-catalyzed carbapenem hydrolysis is distinct from that of penicillin or cephalosporin hydrolysis
Article Snippet: .. The recombinant protein with an N-terminal His6 -tag was produced in Escherichia coli strain BL21(DE3) (Merck, Germany) at 22 °C with an incubation for 16–20 h after induction with 0.5 mM isopropyl β-d -1-thiogalactopyranoside (IPTG). .. Harvested cells were resuspended in the lysis buffer containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM imidazole, 10 mM β-mercaptoethanol, and 1 mM ZnCl2 .

other:

Article Title: Regulation of the rhaEWRBMA Operon Involved in l-Rhamnose Catabolism through Two Transcriptional Factors, RhaR and CcpA, in Bacillus subtilis
Article Snippet: E. coli strain BL21, bearing pCold-rhaA, pCold-rhaB, and pCold-EcrhaB, respectively, was cultivated as mentioned above, from which crude lysates were similarly prepared.

Expressing:

Article Title: Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Article Snippet: .. The expression vector pET24a-hBMP4 was then transformed into the E. coli strain BL21(DE3) (Merck Millipore, USA). ..

Article Title: Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana
Article Snippet: .. Expression and purification of the WT and mutated His-tagged ACA8 N-terminus Vectors coding for WT and mutated His-tagged ACA8 N-terminus (6His-1 M-I116 ) were used to transform E. coli strain BL21(DE3)pLysE (Merck KGaA, Darmstadt, Germany, catalogue no. 69389-3) by standard procedures. ..

Article Title: Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Article Snippet: .. The expression vector pET24a-hBMP4 was then transformed into the E. coli strain BL21(DE3) (Merck Millipore, USA). ..

Transformation Assay:

Article Title: Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Article Snippet: .. The expression vector pET24a-hBMP4 was then transformed into the E. coli strain BL21(DE3) (Merck Millipore, USA). ..

Article Title: Regulation of the rhaEWRBMA Operon Involved in l-Rhamnose Catabolism through Two Transcriptional Factors, RhaR and CcpA, in Bacillus subtilis
Article Snippet: .. E. coli strain BL21 (Merck Millipore), transformed with pCold-rhaR, was grown with shaking in a Luria-Bertani medium ( ) supplemented with ampicillin (50 μg/ml) at 37°C to an OD600 of 0.4. .. The culture was then refrigerated at 15°C and left to stand for 30 min. After IPTG had been added to a final concentration of 1 mM, shaking of the culture was resumed at 15°C and continued for another 24 h. The cells harvested from 6 liters of the culture were disrupted by sonication in buffer 1 (20 mM Tris-Cl buffer [pH 8.0] plus 10% [vol/vol] glycerol, 0.1 mM phenylmethylsulfonyl fluoride, and 1 mM dithiothreitol).

Article Title: Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Article Snippet: .. The expression vector pET24a-hBMP4 was then transformed into the E. coli strain BL21(DE3) (Merck Millipore, USA). ..

Recombinant:

Article Title: De novo GTP Biosynthesis Is Critical for Virulence of the Fungal Pathogen Cryptococcus neoformans
Article Snippet: .. Recombinant Cryptococcus IMPDH was heterologously expressed in E. coli strain BL21(DE3)pLysS (Merck, Darmstadt, Germany). .. MPA, mizoribine, 5-fluoro-2′-deoxyuridine, G418, NAD+ , Tris, Bis-Tris, glycine, NaCl, KCl, DTT, EDTA, DMSO, uric acid, inosine, hypoxanthine, xanthine, xanthosine, guanine, guanosine, adenine, adenosine, IMP, AMP, XMP and GMP were purchased from Sigma (St Louis, MO).

Article Title: The mechanism of NDM-1-catalyzed carbapenem hydrolysis is distinct from that of penicillin or cephalosporin hydrolysis
Article Snippet: .. The recombinant protein with an N-terminal His6 -tag was produced in Escherichia coli strain BL21(DE3) (Merck, Germany) at 22 °C with an incubation for 16–20 h after induction with 0.5 mM isopropyl β-d -1-thiogalactopyranoside (IPTG). .. Harvested cells were resuspended in the lysis buffer containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM imidazole, 10 mM β-mercaptoethanol, and 1 mM ZnCl2 .

Plasmid Preparation:

Article Title: Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Article Snippet: .. The expression vector pET24a-hBMP4 was then transformed into the E. coli strain BL21(DE3) (Merck Millipore, USA). ..

Article Title: Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Article Snippet: .. The expression vector pET24a-hBMP4 was then transformed into the E. coli strain BL21(DE3) (Merck Millipore, USA). ..

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  • 91
    Merck KGaA bl21 de3 plyss strain
    Small Scale expression analysis from pBDDP-SPR3 constructs. GTPases were expressed in the <t>Bl21</t> (DE3) <t>pLysS</t> strain of E. coli in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate. A 1 ml sample of cells was collected before induction and at the time of cell harvesting. This allowed the assessment of total cell protein by SDS-PAGE, and the visualisation of over-expressed target protein. A 40 ml sample of expression culture was applied to a small scale CoNTA IMAC purification to demonstrate the presence of soluble his-tagged protein. (As reported by others, CoNTA spin column purification has proved particularly useful in this respect and gives a consistently clear indication of levels and integrity of recombinant proteins [20] .) In all three cases there was significant soluble expression of the GTPase allowing progression to FPLC scale purification (H 8 -H 8 -KRas 1–169, 24.8 kDa; H 8 -H 8 -Rac1-2-177, 26.2 kDa; H 8 -H 8 -RalB, 25.5 kDa).
    Bl21 De3 Plyss Strain, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 plyss strain/product/Merck KGaA
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 plyss strain - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Merck KGaA escherichia coli strain bl21 de3
    Small Scale expression analysis from pBDDP-SPR3 constructs. GTPases were expressed in the <t>Bl21</t> (DE3) <t>pLysS</t> strain of E. coli in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate. A 1 ml sample of cells was collected before induction and at the time of cell harvesting. This allowed the assessment of total cell protein by SDS-PAGE, and the visualisation of over-expressed target protein. A 40 ml sample of expression culture was applied to a small scale CoNTA IMAC purification to demonstrate the presence of soluble his-tagged protein. (As reported by others, CoNTA spin column purification has proved particularly useful in this respect and gives a consistently clear indication of levels and integrity of recombinant proteins [20] .) In all three cases there was significant soluble expression of the GTPase allowing progression to FPLC scale purification (H 8 -H 8 -KRas 1–169, 24.8 kDa; H 8 -H 8 -Rac1-2-177, 26.2 kDa; H 8 -H 8 -RalB, 25.5 kDa).
    Escherichia Coli Strain Bl21 De3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain bl21 de3/product/Merck KGaA
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 de3 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Merck KGaA bl21
    Small Scale expression analysis from pBDDP-SPR3 constructs. GTPases were expressed in the <t>Bl21</t> (DE3) <t>pLysS</t> strain of E. coli in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate. A 1 ml sample of cells was collected before induction and at the time of cell harvesting. This allowed the assessment of total cell protein by SDS-PAGE, and the visualisation of over-expressed target protein. A 40 ml sample of expression culture was applied to a small scale CoNTA IMAC purification to demonstrate the presence of soluble his-tagged protein. (As reported by others, CoNTA spin column purification has proved particularly useful in this respect and gives a consistently clear indication of levels and integrity of recombinant proteins [20] .) In all three cases there was significant soluble expression of the GTPase allowing progression to FPLC scale purification (H 8 -H 8 -KRas 1–169, 24.8 kDa; H 8 -H 8 -Rac1-2-177, 26.2 kDa; H 8 -H 8 -RalB, 25.5 kDa).
    Bl21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21/product/Merck KGaA
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    bl21 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Merck KGaA e coli bl21
    Small Scale expression analysis from pBDDP-SPR3 constructs. GTPases were expressed in the <t>Bl21</t> (DE3) <t>pLysS</t> strain of E. coli in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate. A 1 ml sample of cells was collected before induction and at the time of cell harvesting. This allowed the assessment of total cell protein by SDS-PAGE, and the visualisation of over-expressed target protein. A 40 ml sample of expression culture was applied to a small scale CoNTA IMAC purification to demonstrate the presence of soluble his-tagged protein. (As reported by others, CoNTA spin column purification has proved particularly useful in this respect and gives a consistently clear indication of levels and integrity of recombinant proteins [20] .) In all three cases there was significant soluble expression of the GTPase allowing progression to FPLC scale purification (H 8 -H 8 -KRas 1–169, 24.8 kDa; H 8 -H 8 -Rac1-2-177, 26.2 kDa; H 8 -H 8 -RalB, 25.5 kDa).
    E Coli Bl21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21/product/Merck KGaA
    Average 93 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 - by Bioz Stars, 2020-08
    93/100 stars
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    Small Scale expression analysis from pBDDP-SPR3 constructs. GTPases were expressed in the Bl21 (DE3) pLysS strain of E. coli in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate. A 1 ml sample of cells was collected before induction and at the time of cell harvesting. This allowed the assessment of total cell protein by SDS-PAGE, and the visualisation of over-expressed target protein. A 40 ml sample of expression culture was applied to a small scale CoNTA IMAC purification to demonstrate the presence of soluble his-tagged protein. (As reported by others, CoNTA spin column purification has proved particularly useful in this respect and gives a consistently clear indication of levels and integrity of recombinant proteins [20] .) In all three cases there was significant soluble expression of the GTPase allowing progression to FPLC scale purification (H 8 -H 8 -KRas 1–169, 24.8 kDa; H 8 -H 8 -Rac1-2-177, 26.2 kDa; H 8 -H 8 -RalB, 25.5 kDa).

    Journal: Protein Expression and Purification

    Article Title: A fully automated procedure for the parallel, multidimensional purification and nucleotide loading of the human GTPases KRas, Rac1 and RalB

    doi: 10.1016/j.pep.2017.01.010

    Figure Lengend Snippet: Small Scale expression analysis from pBDDP-SPR3 constructs. GTPases were expressed in the Bl21 (DE3) pLysS strain of E. coli in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate. A 1 ml sample of cells was collected before induction and at the time of cell harvesting. This allowed the assessment of total cell protein by SDS-PAGE, and the visualisation of over-expressed target protein. A 40 ml sample of expression culture was applied to a small scale CoNTA IMAC purification to demonstrate the presence of soluble his-tagged protein. (As reported by others, CoNTA spin column purification has proved particularly useful in this respect and gives a consistently clear indication of levels and integrity of recombinant proteins [20] .) In all three cases there was significant soluble expression of the GTPase allowing progression to FPLC scale purification (H 8 -H 8 -KRas 1–169, 24.8 kDa; H 8 -H 8 -Rac1-2-177, 26.2 kDa; H 8 -H 8 -RalB, 25.5 kDa).

    Article Snippet: 2.2 Expression of the CDC25 GEF domain of SOS1 and G-domains of KRas 4B, Rac1 and RalB in pBDDP-SPR3 Plasmids were transformed into the BL21 (DE3) pLysS strain of E. coli (Merck Millipore) and the transformed cells were cultured overnight at 37 °C in Luria Bertani broth supplemented with 30 μg/ml kanamycin sulfate (Melford Laboratories Ltd).

    Techniques: Expressing, Construct, Cell Harvesting, SDS Page, Purification, Recombinant, Fast Protein Liquid Chromatography